• Journal of Microbiology and Biotechnology
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• v.22 no.4
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• pp.501-509
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• 2012

A 990 bp full-length gene (xynAHJ2) encoding a 329-residue polypeptide (XynAHJ2) with a calculated mass of 38.4 kDa was cloned from Bacillus sp. HJ2 harbored in a saline soil. XynAHJ2 showed no signal peptide, distinct amino acid stretches of glycoside hydrolase (GH) family 10 intracellular endoxylanases, and the highest amino acid sequence identity of 65.3% with the identified GH 10 intracellular mesophilic endoxylanase iM-KRICT PX1-Ps from Paenibacillus sp. HPL-001 (ACJ06666). The recombinant enzyme (rXynAHJ2) was expressed in Escherichia coli and displayed the typical characteristics of low-temperature-active enzyme (exhibiting optimum activity at $35^{\circ}C$, 62% at $20^{\circ}C$, and 38% at $10^{\circ}C$; thermolability at ${\geq}45^{\circ}C$). Compared with the reported GH 10 low-temperature-active endoxylanases, which are all extracellular, rXynAHJ2 showed low amino acid sequence identities (<45%), low homology (different phylogenetic cluster), and difference of structure (decreased amount of total accessible surface area and exposed nonpolar accessible surface area). Compared with the reported GH 10 intracellular endoxylanases, which are all mesophilic and thermophilic, rXynAHJ2 has decreased numbers of arginine residues and salt bridges, and showed resistance to $Ni^{2+}$, $Ca^{2+}$, or EDTA at 10 mM final concentration. The above mechanism of structural adaptation for low-temperature activity of intracellular endoxylanase rXynAHJ2 is different from that of GH 10 extracellular low-temperature-active endoxylanases. This is the first report of the molecular and biochemical characterizations of a novel intracellular low-temperature-active xylanase.

• Korean Journal of Culture and Social Issue
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• v.21 no.3
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• pp.299-315
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• 2015

This study examines correlations among factors that reportedly affect adjustment to military life: self esteem, locus of control,, social support and stress coping strategies. The study also attempts to validate the structural model to enhance understanding of the process of adjusting to the military life. The subjects of the study were 447 listed soldiers from private to sergeant currently stationed and serving in an army post in Gangwon-Do, Korea. The following tools were used for this study. Based on self-inventory questionnaires, evaluations were made regarding self esteem, locus of control, social support, stress management, adaptation to military life. Reliability of each criterion was evaluated based on reliability examination, correlations among parameters were analyzed, and the structural equation model was validated to verify the research model. The results of the study can be summarized as follows. First, internal control and self esteem have significant positive influence on social support and stress coping. Second, internal control and self esteem affect adjusting to military life through social support and active coping, two factors mediated in this study to act as parameters between self-esteem and internal control and military adaption. This study emphasizes intervention such as establishment of a social support system and training for taking necessary actions as limitations of studies dealing with internal traits and military adaptation. Since the subjects of this study were chosen from an army based in a single location for convenience' sake, it may be difficult to generalize the results to analyze the entire military.

• Journal of the Korea institute for structural maintenance and inspection
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• v.28 no.4
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• pp.55-61
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• 2024

Recently, the number of aging concrete structures is steadily increasing. This is because many of these structures are reaching their expected lifespan. Such structures require accurate inspections and persistent maintenance. Otherwise, their original functions and performance may degrade, potentially leading to safety accidents. Therefore, research on objective inspection technologies using deep learning and computer vision is actively being conducted. High-resolution images can accurately observe not only micro cracks but also spalling and exposed rebar, and deep learning enables automated detection. High detection performance in deep learning is only guaranteed with diverse and numerous training datasets. However, surface damage to concrete is not commonly captured in images, resulting in a lack of training data. To overcome this limitation, this study proposed a method for generating concrete surface damage images, including cracks, spalling, and exposed rebar, using stable diffusion. This method synthesizes new damage images by paired text and image data. For this purpose, a training dataset of 678 images was secured, and fine-tuning was performed through low-rank adaptation. The quality of the generated images was compared according to three base models of stable diffusion. As a result, a method to synthesize the most diverse and high-quality concrete damage images was developed. This research is expected to address the issue of data scarcity and contribute to improving the accuracy of deep learning-based damage detection algorithms in the future.

• Applied Microscopy
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• v.30 no.2
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• pp.233-240
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• 2000

Aspects of structural differentiation of the free -floating hydrophyte, Spirodela polyrhira, has been investigated. The hydrophyte exhibited highly reduced structures having only fronds, connective stalks, and roots as a mature plant. The fronds were major photosynthetic and vegetatively reproducing organ during the growth. Daughter fronds which developed early in the mother frond were also chlorenchymatous, but they remained within foliage sheaths of the mother frond before separation from connecting stalks. Although the stalks and roots originated from the same meristematic region of the frond, they exhibited the distinct polarity showing former lateral growth and latter axial growth. Air chambers were formed only in the fronds and roots. Cellular components were scattered throughout the diffuse cytoplasm in most of the actively growing stalk cells. Root cells protected by the root cap demonstrated relatively complex organization, showing dense cytoplasm with Golgi, rER, mitochondria, and chloroplasts in the cortical cells. Cells in the root cap were highly vacuolated within the peripheral cytoplasm. Such reduction and differentiation of the plant body in Spirodela polyrhiza most effectively contributes to the better adaptation of smaller plants to superficial aquatic environments, while also enabling the rapid growth.

• Korean Journal of Oriental Medicine
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• v.11 no.2
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• pp.23-33
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• 2005

Four Properties and five Flavors of Drugs is interpreted by adaptation of human body to the environmental theory(天人相應). The Structural model of the body is compared with sky, earth, sun and moon (天, 地, 日, 月). The natural changes of the four seasons give rise to that of Four Properties and five Flavors of Drugs. On equal terms it is happened in our body. On this study we can draw an analogy between sky, earth, sun & moon (天, 地, 日, 月) and the body. The six bu(六腑) is related to the earth, the five ju(五主) to the sky, the five jang(五臟) to the sun, the meridians system (經絡) to the moon. When spring, the air is warm, the water element of the earth is ascending, and the earth gives birth to the sour flavor. Like this, the water element is absorbed by six bu and then is ascended to the meridian system. When summer, the air is hot and the water element of the earth is floated, the earth make the bitter flavor. In the same way, the six bu absorbed the hot air from the five ju and the water element is quickly absorbed by six bu and then the water element is ascended to the meridian system. When rainy season (長夏), the earth creates the sweet flavor The sweet flavor give warmer energy to the five jang and the six bu. When autumn, the earth change the sweet flavor into pungent. The earth gives warmer energy to the sky, because of cool weather According to same process, the pungent flavor give warmer energy to the five jang and the six bu, and the meridian system gets back the water element from the five ju. When winter, the air is cold and the water element of the earth is hidden. The sky and the earth are not interchangeable. At that time, the earth produce the salty flavor and the water element is keeping in the meridian system.

• The Journal of the Korea Contents Association
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• v.20 no.5
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• pp.193-203
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• 2020

The purpose of this study is to test the effect of smart-phone addiction on school adjustment. In detail, this study focus on mediating effect of psychological distress. For this purpose, Data Collected on 375 students at 4 high schools in the capital area were used in the analysis. Correlation analysis was conducted to find out the relationship between smart-phone addiction, school adjustment, psychological distress. To find out if smart-phone addiction and school adjustment are mediated by the relationship between psychological distress, the model was tested using a structural equation. The results of the study were as follows: The smart-phone addiction is found to have an adverse effect on high school students' school adaptation and a positive effect on psychological distress. And the results of the verification of the structural model showed a significant mediating effect of psychological distress in the relationship between smart-phone addiction and school adjustment. Based on the results of this study, significance and implications were presented.

• Smart Structures and Systems
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• v.21 no.3
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• pp.359-371
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• 2018

Strong seismic events commonly cause large drift and deformation, and functionality failures in the superstructures. One way to prevent functionality failures is to design structures which are ductile and flexible through yielding when subjected to strong ground excitations. By developing forces that assist motion as "negative stiffness forces", yielding can be achieved. In this paper, we adopt the weakening and damping method to achieve a new approach to reduce all of the structural responses by further adjusting damping phase. A semi-active control system is adopted to perform the experiments. In this adaptation, negative stiffness forces through certain devices are used in weakening phase to reduce structural strength. Magneto-rheological (MR) dampers are then added to preserve stability of the structure. To adjust the voltage in MR dampers, an inverse model is employed in the control system to command MR dampers and generate the desired control forces, where a velocity control algorithm produces initial required control force. An extensive numerical study is conducted to evaluate proposed methodology by using the smart base-isolated benchmark building. Totally, nine control systems are examined to study proposed strategy. Based on the numerical results of seven earthquakes, the use of proposed strategy not only reduces base displacements, base accelerations and base shear but also leads to reduction of accelerations and inter story drifts of the superstructure. Numerical results shows that the usage of inverse model produces the desired regulated damping, thus improving the stability of the structure.

• The Korean Nurse
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• v.31 no.5
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• pp.72-87
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• 1992

This study was designed to develop and lest a structural model for paternal adaplation and family stress in the families with the handicapped children. A hypothetical model was constructed on the basis of family stress theory developed by McCubbin etc. The model included 6 theoretical concepts and 11 paths. For the purpose of the model testing, empirical data were collected from May to August, 1992. The subject of the study constituted 190 mothers whose children admitted in the five special schools and one privale institutes in Seoul and Choong Nam. In data analysis, SPSS PC+ and PC-LISREL 7.13 computer program were utilized for descriptive and covariance structure analysis. The results of lhe study were as follows. (1) Hypothetical model showed a good fit to the empirical data [Chi-square = 21.19 (df = 7, P = o. 003), Goodness of Fit Index=O.986, Adjusted Goodness of Fit Indes=0.946, Root Mean Square lesidual=0.048), Non Normed Fit Index = 0.80, Normed Fit Index=0.91]. (2) The results of Hypothesis testing indicated: 1) Social support(${\gamma}_11=.238$, T=2.352), Family Functioning(${\gamma}_12=.729$, T=5.957) had direct effects on situational definition. 2) life event stress(${\gamma}_23=.284$, T=5.220) had direct effects on the effect of the handicapped chid on the family. 3) Family functioning(${\gamma}_32=-.239$, T= -3.370) had direct effects on paternal adapation. From the above results, the significance of this study can be delineated as follows : The construction and testing of the comprehensive model seem to be the first trial in Korea. The model was supported by empirical dala. Thus it was suggested that model could be adequately applied to framily nusing care with the handicapped.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.3
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• pp.157-163
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• 1995

The objective of this study is to obtain the basic data for investigating the effects of organic reserves on winter survial or regrowth yield. Dry matter, nitrogen and non-structural carbohydrate content of plants grown under $5^{\circ}C$ or $20^{\circ}C$ of culture temperature during 25 days were investigated. The dry matter content of leaves and roots were significantly reduced under $5^{\circ}C$ compared with $20^{\circ}C$culture condition. Comparing with the dry matter per plant under $20^{\circ}C$, those in leaves and roots under $5^{\circ}C$ decreased to 25% and 10%, respectively, after 25 days of temperature treatment. Total nitrogen content in leaves under $20^{\circ}C$ and $5^{\circ}C$ increased to 68% and 39% compared to the initial lenel(day O), respectively, during 25 days after temperature treatment, Nitrogen content in roots highly increased under 5 C while there was a little change under $20^{\circ}C$ condition. The nitrogen contents in roots under $5^{\circ}C$ and $20^{\circ}C$ were 39.0 and 30.8mgJg DM, respectively, after 25 days of temperature treatment. Total contents of soluble carbohydrate in both leaves and roots under $5^{\circ}C$ were higher than those under $20^{\circ}C$ condition. After 25 days of temperature treatment under$5^{\circ}C$ , their contents in leaves and roots were 1.4 and 2.0 times higher than those of under $20^{\circ}C$ condition. Stach atent in roots under $20^{\circ}C$ was less changed, while thatof under $5^{\circ}C$ greatly increased from 64.8 to 178.7mglg DM duling 25 days. 'Ihese results clearly showed that an accumulation of both nitrogen and non-structural carbohydrate in the plants occured under low temperature condition.e condition.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.4
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• pp.259-264
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• 2001

To investigate the physiological responses to naturally occurring winter freezing stress in creeping bentgrass, changes in carbohydrates were monitored during winter period. Turf quality and leaf growth was nearly parallel with temperature fluctuation. The concentration of glucose, fructose and sucrose in both shoot and root gradually increased from November to January, and then sharply decreased until April. Sucrose was the largest pool of soluble sugars. Fructan also slightly accumulated in both shoot and roots from November to February. Fructan hydrolysis in both organs was found to be much active between February to April. Shoot contained largely higher carbohydrate content in all compounds examined than roots did. Fructan was found to be a main carbohydrate storage form, showing the highest concentration (176.7 and 126.7 mg g-' DW for shoot and root in February). The depolymerization of fructan from February coincided with the high declines in mono- and disaccharide. These results suggest that the accumulation of non-structural carbohydrate until January could be associated with freezing tolerance, and the active decrease from February with shoot regrowth.