• The Korean Journal of Mycology
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• 제31권1호
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• pp.1-7
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• 2003

One hundred fifty one specimens of Beauveria spp. from 19 different locations were collected from September 1 to August 31, 2002. Most of the isolates were identified as Beauveria. bassiana. Cordyceps staphylinidaecola collected from Mt. Obong in Chunchon City covered the host with mycelia which were produced 1 to 4 stromata along with asexual spores. The size of bright yellow ununiform stromata were about 45 mm and the head about $17mm{\times}4mm$. Perithecia completely immersed were $530{\sim}550{\times}290{\sim}300{\mu}m$ in size and mainly scattered on the head. Ascospore produced in asci in the size of $400{\sim}450{\times}4{\sim}5{\mu}m$ developed thread-like secondary spores, which were directly separated into secondary conidial spores. Conidia produced at apical portion of synnemata were $2.6{\sim}3.4{\times}1.2{\sim}1.9{\mu}m$ in size. High density of mycelium was observed at $25^{\circ}C$ ranged from pH 6.5 to 8.5 after 11 days of inoculation. It took 15 to 18 days after inoculation to fully grow on the medium mixed brown rice with pupa. Mycelium developed stromata on the medium 30 days after completion of mycelial growth, where perithecia were produced in 40 days.

• The Korean Journal of Mycology
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• 제27권5호
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• pp.341-344
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• 1999

The genus Hypocrea, a member of the Hypocreales, has yellow to pale yellow-ochre, perithecial stromata and angular to cuboid ascospores. The isolate of Hypocrea microrufa collected on decayed tropical wood at Malaysia is compared with H. rufa and H. microsplendens. This isolation of H. microrufa will be the first record from tropical region and the first description with scanning electron microscopy. The ornamentation of ascospores used one of main taxonomic keys for identification.

• Applied Microscopy
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• 제34권4호
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• pp.271-276
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• 2004

The genus of Biscogniauxia, well known wood degrading fungi, is a member of the Xylariales, which has woody to carbonaceous, brown to dark brown stromata. Daldinia concentrica and Biscogniauxia sp. isolated from heavily decayed hardwood, are precisely described under light microscopic and scanning electron microscopic level. Daldinia concentrica will be the first ultrastructural description in Korea. The unidentified species collected, having small size of ascospores $11.4\;15.6{\times}9.6\;12.0{\mu}m$ with full germ slit, are taxono-mically compared with similar species found in Korea.

• Mycobiology
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• 제34권1호
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• pp.1-6
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• 2006

A Cordyceps species was found with a Beauveria anamorph state on larval insect cadavers on Obong mountains in Gangwon Provinces, Republic of Korea. Cultures from discharged ascospores formed an anamorph identifiable as Beauveria bassiana. This teleomorph-anamorph connection was also confirmed by the in vitro production of fertile ascomata from conidial cultures with morphology like that of field-collected specimen. This is the first report of in vitro production of a teleomorph for any Beauveria species. The Cordyceps species has been conspecified as Cordyceps bassiana, a species described from China with B. bassiana anamorph.

• The Korean Journal of Mycology
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• 제14권1호
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• pp.85-88
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• 1986

More than 40 samples of the genus Cordyceps were collected and investigated. Among them, six species were confirmed as unrecorded ones in Korea. Unrecorded species were Cordyceps alboperitheciata, Cordyceps discoideocapitata, Cordyceps intermedia, Cordyceps nigrella, Cordyceps roseostromata and Cordyceps scarabaeicola.

• International Journal of Industrial Entomology and Biomaterials
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• 제21권1호
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• pp.117-125
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• 2010

The tropical tasar silkworms, Antheraea mylitta (D) produce famous silk 'Kosa' in central part of India. Due to outdoor rearing it became susceptible to viral infection including cytoplasmic polyhedrosis virus (CPV). The common mode of entry of cytoplasmic polyhedrosis virus is per os and cause gresserie disease to the larvae. Histopathological studies elucidated the insect CPV virus produces infective polyhedral inclusion bodies (PIBs) in the midgut cell cytoplasm of virus infected fifth instar larvae. The PIBs multiply enormously in the cytoplasm without invading the nucleus. Ultrastructural studies confirmed the pathological effects of CPV on in midgut cell cytoplasm. The multiplication of polyhedral inclusion bodies took place into the vacuoles and form virogenic stromata in the cytoplasm of cells. However, the encapsulations of polyhedral inclusion bodies into the polyhedrin protein occurred and polyhedra were released into the lumen. At the late stage of infection, cells showed the regressed cytoplasmic organelles with large vacuoles and elongated mitochondria. Hence, the horizontal transmission of CPV causing the midgut cells disintegration in the tasar silkworm, Antheraea mylitta (D) confirmed during infection.

• Mycobiology
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• 제47권3호
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• pp.280-291
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• 2019

To explore species diversity of Hypocreaceae, collections from Guangdong, Hubei, and Tibet of China were examined and two new species and a new Chinese record were discovered. Morphological characteristics and DNA sequence analyses of the ITS, LSU, $EF-1{\alpha}$, and RPB2 regions support their placements in Hypocreaceae and the establishments of the new species. Hypomyces hubeiensis sp. nov. is characterized by occurrence on fruitbody of Agaricus sp., concentric rings formed on MEA medium, verticillium-like conidiophores, subulate phialides, rod-shaped to narrowly ellipsoidal conidia, and absence of chlamydospores. Trichoderma subiculoides sp. nov. is distinguished by effuse to confluent rudimentary stromata lacking of a well-developed flank and not changing color in KOH, subcylindrical asci containing eight ascospores that disarticulate into 16 dimorphic part-ascospores, verticillium-like conidiophores, subcylindrical phialides, and subellipsoidal to rod-shaped conidia. Morphological distinctions between the new species and their close relatives are discussed. Hypomyces orthosporus is found for the first time from China.

• The Korean Journal of Mycology
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• 제32권2호
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• pp.79-88
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• 2004

Cordyceps pruinosa grows upon dead pupae of Lepidoptera and produces one or $3{\sim}4$ club-shaped stromata per host. The stromata have distinct club-shaped head and long stalk. The length of stromata varies from $1{\sim}3\;cm$. Apical head consists of densely crowded semi-immersed perithecia, which are $360{\sim}400\;{\times}\;180{\sim}200\;{\mu}m$ in size. Asci are $150\;{\mu}m$ in length and $2.8{\sim}3\;{\mu}m$ in diameter. Ascospores, which are $124{\sim}141\;{\mu}m$ in length, have thin thread-like structures in the middle with part-spores attached on both sides. Each ascospore does not separate into part-spores after dispersal, but each part-spore germinates and together develops a colony. The imperfect form produces phialides of $15{\sim}24\;{\times}\;2{\sim}3\;{\mu}m$ size, with spherical or spindle shaped conidia of $4{\sim}6\;{\times}\;1.8{\sim}2.4\;{\mu}m$ size, The anamorph was identified as Mariannaea elegans Samson. YMA and SDAY agar media with pH 7 was produced abundant mycelial growth with high density. Best mycelial growth was observed when dextrin was used as a carbon source. Lactose, saccharose and sucrose also produced high mycelial growth. Peptone, yeast extract and tryptone produced abundant mycelial growth, when used as nitrogen sources. Highest mycelial growth and density was observed when C/N ratio was 1 : 1 at the concentration of 12.5 g/l each. $KH_2PO_4$ was the best mineral source for mycelial growth. Highest mycelial dry wt. was produced in YM and SDAY broths. Optimum inoculum for 100 ml of liquid broth was 6 mycelial discs. Similarly, optimum liquid culture period was 7 days.

• Journal of Sericultural and Entomological Science
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• 제47권1호
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• pp.18-30
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• 2005

Morphological characteristics of Cordyceps species and its allies collected in Korea were clarified. Through the survey conducted from June 1999 to October 2002 in 19 mountains in Korea, 667 samples of entomogenous fungi were collected. Cordyceps and its allies of 17 species of 5 genera were identified as Cordyceps gracilioides, C. japonica, C. longissima, C. martialis, C. militaris, C. myrmecophila, C. nutans, C. pruinosa, C. sphecocephala, C. tricentri, Hirsutella nutans, Paecilomyces cicadae, P. farinosus, P. tenuipes, Paecilomyces sp., Shimizuomyces paradoxa, Tilachlidiopsis nigra. The fungi with insect hosts have been collected mainly in the place of shade or mosses near brooks and streams that had high humidity. Overall the frequenct of fungal infection in natural ecosystem was relatively low as few as 10 collections per each species. However, many species were found in terms of the few number of colleciton sites with seasonal limitations. Occurrence of the fungi in Jeju island remote from inland of the Korean peninsula were diverse in their species due to the varied weather of vertical distribution following the altitude. Three most common species were C. nutans, P. tenuipes and C. militaris, mainly found early in August when the relative humidity and temperature were high, of which C. nutans occupied the highest frequency consisting of 65% in total collections. Neither variation in ascomata arrangement in stromata nor developement of secondary spores was recognizable, while the number, shape and colour of stromata varied with insect hosts and weather conditions.

• The Korean Journal of Mycology
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• 제21권2호
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• pp.94-105
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• 1993

The genus Cordyceps known as an insect parasite forms a sclerotium in insect bodies and then produces perithecia on the single or multiple stromata produced from sclerotium. Collected Cordyceps were identified into 5 species: Cordyceps militaris, C. nutans, Cordyceps sphecocephala, Isaria japonica, and Torrubiella sp. The fruit bodies of Cordyceps in petri-dish cover were fixed by tape and put the lid on water agar plates to isolate these collected Cordyceps. The germinated spores were transferred from water agar to Potato dextrose agar(PDA) after six hours. Mycelial growth of C. nutans and C. militaris was the most successful on Hamada media and was also good on Complete media and PDA. Mannose as a carbon source was good for two species and Glutamic acid as a nitrogen source was satisfactory to C. militaris and Asparagine gave a good result to C. nutans. C. militaris and C. nutans showed similar mycelial growth rate on the media that contained thiamine-HCI, biotine or nicotinic acid as a vitamine. When conidia of C. nutans were inoculated to insects, mortality was high in Artogeia napi L, Hemiptera, Plutella xylostella and 50% in Orthoptera, 12% in Acantholyda posticalise M, but not Agelastica coerulea B. in Aphididae, C. nutans was collected from only Hemiptera in nature, but killing effect on other insects was proved. Mycelial growth and fruit-body formation were good on the media that consist of rice powder 5g, wheat flour 5g, water 100ml, but formed fruit-body was not complete stromata but a mass of conidia according to results of observing microscope.