• Journal of Life Science
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• v.28 no.8
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• pp.931-937
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• 2018

Triptolide is a compound found in Tripterygium wilfordii and reported to have an anti-inflammatory and anti-oxidant activities. A previous study shows that the dietary supplementation with triptolide increases resistance to environmental stressors, including oxidative stress, heat shock, and ultraviolet irradiation, and extends lifespan in C. elegans. Here, we investigated the underlying mechanisms involved in the lifespan-extending effect of triptolide. The effect of triptolide on age-related diseases, such as diabetes mellitus and Alzheimer's disease, was also examined using animal disease models. The longevity phenotype conferred by triptolide was not observed in the eat-2 mutant, a well-known genetic model of dietary restriction, while there was an additional lifespan extension with triptolide in age-1 and clk-1 mutants. The long lifespan of age-1 mutant is resulted from a reduced insulin/IGF-1-like signaling and the clk-1 mutant lives longer than wild-type due to dysfunction of mitochondrial electron transport chain reaction. The effect of dietary restriction using bacterial dilution on lifespan also overlapped with that of triptolide. The toxicity of high glucose diet or transgenic human amyloid beta gene was significantly suppressed by the supplementation with triptolide. These findings suggest that triptolide can mimic the effect of dietary restriction on lifespan and onset of age-related diseases. We conclude that triptolide can be a strong candidate for the development of dietary restriction mimetics.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.10
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• pp.4660-4668
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• 2012

The purpose of this study was to investigate the relationship among stress, ego-resilience and school adjustment resilience of the high school girl students. A cross-sectional, descriptive study was conducted. The subjects were 250 students from 3 high schools located in I city. Data were collected on September, 2010 by using a structured questionnaire. The data were analyzed using PASW/WIN 18.0 program. The mean score of stress was 3.05, among the subitems, the score of the school related stress was the highest. The mean score of ego resilience was 2.86, and school adjustment resilience was 75.58. Stress showed significant differences according to satisfaction level with school and teachers. School adjustment resilience was significantly different in terms of satisfaction level with school and teachers, grade, alcohol drinking experience and having counter sex friends. And there were significant correlations among school adjustment resilience and school related, family related, friend related stress. These results suggest that the school life, teachers, and friends have important duties to improve school adjustment resilience. Therefore, it is needed to develop intervention program considerated school related factors to release stressors of the high school girl students.

• Journal of Oral Medicine and Pain
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• v.37 no.1
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• pp.9-17
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• 2012

Lichen planus is a chronic inflammatory mucocutaneous disease that affects multiple sites of the body. Often it involves the oral mucosa, but also involve other sites such as skin, genitals, scalp and nails. There is no clear cause of oral lichen planus (OLP), current data suggest that OLP is a T-cell mediated autoimmune disorder which may have an altered self-peptide triggering apoptosis of oral epithelial cells. Usually OLP appears in middle-aged women which tends to be chronic with periods of exacerbation and remission. There are many theories those causes the OLP such as psychological and environmental factors, genetic tendency, drugs and more. 60-70% of lichen planus is accompanied by oral lesions, and more than half of its cases are not able to defined by their skin. In this study, among all the possibility(possible) theories, we tried to evaluate the influence of emotional stress in exacerbating OLP. There were thirty patients with a clinical or histological diagnosis of OLP and other thirty subjects who did not show any signs of systemic disorders include OLP. They were evaluated by using modified Holmes and Rahe's Social Readjustment Rating Scale (SRRS). As a result, a significantly higher level of stress was found in the OLP patients than the control group. Therefore it could be concluded that psychological stressors play an important role in the exacerbating OLP.

• Ocean and Polar Research
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• v.36 no.2
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• pp.121-134
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• 2014

Sessile marine bivalves including mussels, oysters and clams are often used as a sentinel species in coastal environmental monitoring since changes in the environmental quality are often well preserved in their tissues and shells. In this study, we investigated overall health condition of the Mediterranean Mussel, Mytilus galloprovincialis on the south coast using histology. Reproductive condition as gonad index (GI), condition index (CI) as a ratio of the tissue weight to the shell weight, digestive gland atrophy (DGA), types of parasites, and pathologic conditions including erosion, necrosis, hemocyte infiltration, and neoplasia were examined from each histological preparation. GI decreased from March to July then increased from July to September and spawning mussel could be observed as early as in April and the activity continued until September. CI also followed the monthly changes in GI, indicating that decrease in CI was associated with the weight loss due to spawning. DGA increased from March to June, decreased in July and increased from July to September. High DGA values observed in June and September were coincided with spawning and high water temperature. Histology also showed high prevalence of erosion in the digestive gland in June (36.0%) and September (56.4%), suggesting that high water temperature and spawning acted as environmental stressors. No parasitic organism was identified during the survey, although some symbiotic copepods were observed. Histology was found to be useful and affordable technique in monitoring the overall health of mussel, providing useful pathologic information of the cells and tissues.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.20 no.1
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• pp.10-21
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• 2021

This study analyzed how external variables, such as the supply policy of shared bicycles and the spread of infectious diseases, affect the demand for shared bicycle use in the COVID-19 era. In addition, this paper presents a methodology for more accurate predictions. The Seasonal Auto-Regulatory Integrated Moving Average with Exogenous stressors methodology was applied to capture the effects of exogenous variables on existing time series models. The exogenous variables that affected the future demand for shared bicycles, such as COVID-19 and the supply of public bicycles, were statistically significant. As a result, from the supply volume and COVID-19 outbreak according to the scenario, it was estimated that approximately 46,000 shared bicycles would be supplied by 2022, and the COVID-19 cases would continue to be at the current level. In addition, approximately 32 million and 45 million units per year will be needed in 2021 and 2024, respectively.

• Journal of Animal Science and Technology
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• v.46 no.5
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• pp.879-886
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• 2004

Effects of culture mediwn supplements and osmotic stress treatment on embryogenic callus induction and somatic embryogenesis were investigated in order to optimize tissue culture conditions of alfalfa(Medicago sativa L.). SH mediwn containing 5mgIL 2,4-D and 0.2mgIL kinetin was optimal for embryogenic callus induction from cotyledon tissue of alfalfa. Somatic embryos were formed when the embryogenic callus was cultured on SH mediwn supplemented with ImgIL 2,4-D and 2mgIL BA. Supplementation of 5mM L-proline and IgIL casein hydrolysate into the regeneration mediwn further increased plant regeneration frequency. Osmotic stress treatment of callus appeared to improve the frequency of somatic embryo formation, but the frequency of somatic embryo formation differed by the osmotic stress treatment using different osmotic stressors. The highest plant regeneration frequency of 30.7% was observed when embryogenic callus was treated with 0.7M sucrose for 18h. Efficient regeneration system established in this study will be useful for molecular breeding of alfalfa through genetic transformation.

• Korean Journal of Social Welfare Studies
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• v.43 no.1
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• pp.389-411
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• 2012

A new era of research has focused on examining the growth of change in drinking problems among the elderly. Thus, the purpose of the present study was two fold: (1) to investigate trajectories of drinking problems(CAGE) among the Korean elderly(age$${\geq_-}65$$); and (2) to identify the predicting factors for the intercept and the slope of alcohol problems using multi-level growth curve model. Data come from three waves(1st wave(2006)~3rd wave(2008) of the Korea Welfare Panel(KWP) study. The results indicated that the levels of drinking problems decreased over time and that age, gender, marital status, religion, poverty, self-rated health, and social relationship satisfaction were associated with the baseline CAGE. Further analysis showed that social relationship satisfaction affected the declining slope of drinking problems over time. Specifically, among those who satisfied social relationship, there was a sharp decline of CAGE over time. Overall findings highlight the importance of developing and implementing effective alcohol prevention programs for the elderly in the community settings to mitigate the harmful effects of various psycho-social stressors. Especially, programs to maintain and form healthy social support network are suggested as critical interventions for prevention as well as recovery of alcohol problems in late life.

• Journal of the Korean Applied Science and Technology
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• v.41 no.2
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• pp.459-471
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• 2024

In modern society, where tension and stress are ubiquitous, individuals often experience psychological imbalances. These stressors not only affect mental well-being but also manifest physically, through the skin. Consequently, a new term psychodermatology combining psychiatry and dermatology, has emerged, garnerning attention and research focus. In this study, we aimed to develop materials improving chronic skin conditions caused by stress by utilizing a compound of Cucurbita moschata, Paeonia japonica, and Prunus cerasus known to alleviate skin disorders. We sought to develop and validate the efficacy of materials alleviating chronic skin conditions induced by stress in keratinocytes..Therefore, in this study we analyzed the effects of a complex extract using Cucurbita moschata, Paeonia japonica, and Prunus cerasus on HaCaT keratinocyte cells to understand how it influences them. The complex extract on HaCaT keratinocyte cells showed a concentration-dependent decrease in the expression levels of TNF-α, IL-1β, IL-6, MDC, and TARC at concentrations of 12.5, 25, 50 and 100 ㎍/mL. Particularly noteworthy was the efficacy observed in inhibiting IL-1β, with a reduction of over 40% at a concentration of 100 ㎍/mL. Additionally, the production levels of AQP-3, HA, and filaggrin exhibited a significant concentration-dependent increase. The protein expression of p-ERK, p-JNK, and p-p38, which were elevated by TNF-α/IFN-γ, was significantly decreased with the treatment of the complex extract. These findings suggest that the compound extract may be utilized as a material for treating and preventing skin conditions, potentially mitigating the adverse effects of the mutual relationship between skin disorders and stress.

• Journal of Family Resource Management and Policy Review
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• v.16 no.4
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• pp.171-194
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• 2012

This study focuses on immigrant women who are married to Korean men and who live in a multicultural family situation in Gyeonggi-do. These women experience acculturative stress and marital conflict and this study aims to determine how social support seeks to mediate the effects of those stressors. The women in this study participated in activities and received services from one of Gyeonggi-do Province's civic organizations, religious organizations, or social service organizations, such as the Social Welfare Agency and the Multicultural Family Support Center. In order to verify the study's hypothesis, the researchers used the following statistical analytical methods : t-test, two-way ANOVA and multi-regression analysis. Analysis of the study's results showed that the highest degree of marital conflict was found in the sub-zones and variables that were personal. Those variable were : the difference in mindset and values (personal domain), economic problems (communal living area), a child's upbringing and education issues (third party area), and the participant's sex life (in the marital relationship). The hypothesis was tested using the research model validation and the results are summarized as follows. First, in order to analyze the relationship between the marriage migration females' acculturative stress and the impact of that stress on marriage conflict, the hierarchical regression analysis was used. It identified that a direct correlation existed between acculturative stress and marriage conflict; where a higher degree of acculturative stress was present, a higher degree of marriage conflict was found. Second, the study found a statistical significance in the correlation and relationship (${\beta}$=.208, p<0.05) between the acculturative stress of the marriage migration female and material support. In other words, the material support can be seen as having a moderating effect on the acculturative stress, which is the independent variable, and the marital conflict, which is the dependent variable.

• Journal of Life Science
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• v.26 no.12
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• pp.1360-1366
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• 2016

Heat shock proteins (HSPs) are induced in response to various physiological or environmental stressors. However, the transcriptional activation of HSPs is regulated by a family of heat shock factors (HSFs). Fish models provide an ideal system for examining the biochemical and molecular mechanisms of adaptation to various temperatures and water environments. In this study, we examined the pattern differentials of heat shock factor 1 (HSF1) and expression of heat shock protein 70 (HSP70) in response to thermal stress in goldfish and mouse hepatocyte cultures by immune-blot analysis. Goldfish HSF1 (gfHSF1) changed from a monomer to a trimer at $33^{\circ}C$ and showed slightly at $37^{\circ}C$, whereas mouse HSF1 (mHSF1) did so at $42^{\circ}C$. This experiment showed similar results to a previous study, indicating that gfHSF1 and mHSF1 play different temperature in the stress response. We also examined the activation conditions of the purified recombinant proteins in human HSF1 (hmHSF1) and gfHSF1 using CD spectroscopy and immune-blot analysis. The purified recombinant HSF1s were treated from $25^{\circ}C$ to $42^{\circ}C$. Structural changes were observed in hmHSF1 and gfHSF1 according to the heat-treatment conditions. These results revealed that both mammal HSF1 (human and mouse HSF1) and fish HSF1 exhibited temperature-dependent changes; however, their optimal activation temperatures differed.