• Journal of Korean Academy of Nursing
• /
• v.39 no.3
• /
• pp.357-365
• /
• 2009

Purpose: This study was done to examine the effects of aromatherapy on stress and stress responses in adolescents. Methods: A two-group cross-over design was used for this study. The experimental treatment was aroma essential oil inhalation and the placebo treatment was carrier oil inhalation using a necklace. The sample included 36 female high school students. Fisher's exact test, t-test, and paired t-test using SPSS/WIN program were used to analyze the data. Results: Stress levels were significantly lower when the students received the aroma treatment compared to when they received the placebo treatment. The stress responses except salivary IgA levels were significantly lower when the students received the aroma treatment. Conclusion: Aroma inhalation could be a very effective stress management method for high school students. Therefore, it is recommended that this program be used in clinical practice as an effective nursing intervention for high school students.

• Molecules and Cells
• /
• v.39 no.3
• /
• pp.179-185
• /
• 2016

Posttranscriptional regulation of RNA metabolism, including RNA processing, intron splicing, editing, RNA export, and decay, is increasingly regarded as an essential step for fine-tuning the regulation of gene expression in eukaryotes. RNA-binding proteins (RBPs) are central regulatory factors controlling posttranscriptional RNA metabolism during plant growth, development, and stress responses. Although functional roles of diverse RBPs in living organisms have been determined during the last decades, our understanding of the functional roles of RBPs in plants is lagging far behind our understanding of those in other organisms, including animals, bacteria, and viruses. However, recent functional analysis of multiple RBP family members involved in plant RNA metabolism and elucidation of the mechanistic roles of RBPs shed light on the cellular roles of diverse RBPs in growth, development, and stress responses of plants. In this review, we will discuss recent studies demonstrating the emerging roles of multiple RBP family members that play essential roles in RNA metabolism during plant growth, development, and stress responses.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.235-235
• /
• 2017

Chromium (Cr) toxicity is hazardous to the seed germination, growth, and development of plants. ${\gamma}$-Aminobutyric acid (GABA) is a non-protein amino acid and is involved in stress tolerance in plants. To investigate the effects of GABA in alleviating Cr toxicity, we treated eight-d-old mustard (Brassica juncea L.) seedlings with Cr (0.15 mM and 0.3 mM $K_2CrO_4$, 5 days) alone and in combination with GABA ($125{\mu}M$) in a semi-hydroponic medium. The roots and shoots of the seedlings accumulated Cr in a dose-dependent manner, which led to an increase in oxidative damage [lipid peroxidation; hydrogen peroxide ($H_2O_2$) content; superoxide ($O{_2}^{{\cdot}-}$) generation; lipoxygenase (LOX) activity], MG content, and disrupted antioxidant defense and glyoxalase systems. Chromium stress also reduced growth, leaf relative water content (RWC), and chlorophyll (chl) content but increased phytochelatin (PC) and proline (Pro) content. Furthermore, supplementing the Cr-treated seedlings with GABA reduced Cr uptake and upregulated the non-enzymatic antioxidants (ascorbate, AsA; glutathione, GSH) and the activities of the enzymatic antioxidants including ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), glutathione reductase (GR), glutathione peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT), glyoxalase I (Gly I), and glyoxalase II (Gly II), and finally reduced oxidative damage. Adding GABA also increased leaf RWC and chl content, decreased Pro and PC content, and restored plant growth. These findings shed light on the effect of GABA in improving the physiological mechanisms of mustard seedlings in response to Cr stress.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.11
• /
• pp.1787-1795
• /
• 2015

The transition from primary to secondary metabolism in antibiotic-producing Streptomyces correlates with expression of genes involved in stress responses. Consequently, regulatory pathways that regulate specific stress responses are potential targets to manipulate to increase antibiotic titers. In this study, genes encoding key proteins involved in regulation of the osmotic stress response in Streptomyces avermitilis, the industrial producer of avermectins, are investigated as targets. Disruption of either osaB_Sa, encoding a response regulator protein, or osaC_Sa, encoding a multidomain regulator of the alternative sigma factor SigB, led to increased production of both oligomycin, by up to 200%, and avermectin, by up to 37%. The mutations also conditionally affected morphological development; under osmotic stress, the mutants were unable to erect an aerial mycelium. In addition, we demonstrate the delivery of DNA into a streptomycete using biolistics. The data reveal that information on stress regulatory responses can be integrated in rational strain improvement to improve yields of bioactive secondary metabolites.

• International conference on construction engineering and project management
• /
• 2020.12a
• /
• pp.237-242
• /
• 2020

Construction noise is among the most critical stressors that adversely affect the quality of life of the people residing near construction sites. Many countries strictly regulate construction noise based on sound pressure levels, as well as timeslots and type of construction equipment. However, individuals react differently to noise, and their tolerance to noise levels varies, which should be considered when regulating construction noise. Although studies have attempted to analyze individuals' stress responses to construction noise, the lack of quantitative methods to measure stress has limited our understanding of individuals' stress responses to noise. Therefore, the authors proposed a quantitative stress measurement framework with a wearable electroencephalogram (EEG) sensor to decipher human brain wave patterns caused by diverse construction stressors (e.g., worksite hazards). This present study extends this framework to investigate the feasibility of using the wearable EEG sensor to measure individuals' emotional stress responses to construction noise in a laboratory setting. EEG data were collected from three subjects exposed to different construction noises (e.g., tonal vs. impulsive noises, different sound pressure levels) recorded at real construction sites. Simultaneously, the subjects' perceived stress levels against these noises were measured. The results indicate that the wearable EEG sensor can help understand diverse individuals' stress responses to nearby construction noises. This research provides a more quantitative means for measuring the impact of the noise generated at a construction site on neighboring communities, which can help frame more reasonable construction noise regulations that consider various types of residents in urban areas.

• Journal of the Korea Safety Management & Science
• /
• v.10 no.4
• /
• pp.57-63
• /
• 2008

The purpose of this paper is to review the moderating effect of social support on the relationship between job stress responses(physiological response, psychological response, behavioral response) and occupational accidents/illness. Based on the responses from 187 employees in small & medium manufacturing industry, hierarchical regressional analysis showed that physiological response has positive relationship with accidents and illness, and psychological response has positive relationship with accident. Social support has partly moderating effect on the relationship between job stress responses and occupational accidents/illness.

• Proceedings of the Safety Management and Science Conference
• /
• 2008.11a
• /
• pp.317-327
• /
• 2008

The purpose of this paper is to review the relationship between job stress responses(physiological response, psychological response, behavioral response) and occupational accidents/illness, and the moderating effect of social support. Based on the responses from 187 employees in small & medium manufacturing industry, hierarchical regressional analysis showed that physiological response has positive relationship with accidents and illness, and psychological response has positive relationship with accident. Social support has partly moderating effect on the relationship between job stress responses and occupational accidents/illness.

• Journal of Korean Biological Nursing Science
• /
• v.4 no.2
• /
• pp.79-92
• /
• 2002

This study was aimed to analyze the variables measuring stress and immune responses, to identify the relationship between stress and immune responses, and to find out the effect of nursing interventions associated with stress and immune responses by reviewing thirty-four published articles since 1970 in Korea. The articles were selected in the field of nursing, stress management, and masters or doctoral dissertations and limited to human subject. Among these, the thirty-one articles were published since 1996 and mainly distributed in nursing (44.1%) and medicine(44.1%). The prevailing research design was nonequivalent control pre-post experimental design(41.1%). The research subjects were 55.9% for patients and 44.1% for healthy general persons including 20.6% of university students. To evaluate stress, both physiologic and psychosocial measures were adapted together in 35.3% of the articles. The most frequent two variables measuring stress and immune response were cortisol level(15.9%) and number or activity of natural killer cell(25.9%). The relation between stress and immune responses was positive in 4 articles, negative in 9 cases, and none in 12 cases. Decreased stress and enhanced immune function have been found when massage, abdominal breathing, exercise, relaxation, and touch were provided as nursing interventions. The articles to investigate the relationship between stress and immune function were limited and the tested variables were diverse. Also there was no consistent evidence to correlate the stress and immune function at present. Further studies are needed to construct a valid research design and to investigate the relationship between stress and immune responses. Nursing interventions to decrease stress should be developed to result in the increased immune function and the effect of these interventions would be verified.

• Journal of the Korean Society of School Health
• /
• v.28 no.2
• /
• pp.56-66
• /
• 2015

The purpose of this study was to investigate factors associated with coping responses in Korean public high school students. Methods: This study employed a descriptive survey research design. The sample included 263 high school students who responded to a self-report questionnaire. Variables included socio-demographic characteristics, health-related characteristics, academic stress, somatization symptoms, social support, and coping responses. Data were analyzed using descriptive statistics, t-tests, ANOVA, Pearson's correlations, and multiple regressions. Results: Participants, regarding their school life, reported moderate levels of academic stress ($M{\pm}SD=2.3{\pm}0.52$) and somatization symptoms ($M{\pm}SD=2.3{\pm}0.71$), and a relatively high level of social support ($M{\pm}SD=4.2{\pm}0.67$). All the variables were associated with the use of multiple coping responses. Active-cognitive coping ($M{\pm}SD=2.9{\pm}0.68$) was most frequently used, followed by active-behavioral coping ($M{\pm}SD=2.5{\pm}0.56$). and avoidant coping ($M{\pm}SD=2.3{\pm}0.75$). Significant relationships were found among the measured variables: positive relation between academic stress and somatization symptoms, but, negative between academic stress and both somatization symptoms and social support. Students who had higher stress and more somatization symptoms were more likely to use avoidant coping than the others. In multiple regression analysis, while factors associated with each coping response differed, gender appeared to be a significant factor in all methods. Variables included in the final model explained 27% of the variance in avoidant coping (F=11.40, p<.001). Conclusion: Based on the study results, schools should provide tailored educational programs to help high school students reduce multisource stress and somatization symptoms at school and cope with them in more active and effective ways.

• Animal cells and systems
• /
• v.6 no.1
• /
• pp.1-12
• /
• 2002

A role of Src family protein Tyrosine kinases (SFK) as mediators of receptor-ligand initiated responses is well established. Well documented, but less well understood is the role of SFK in cellular reaction to stresses. Evidence from the wide variety of experimental systems indicates that SFK mediate responses to all major classes of stress, including oxidation, DNA damage, mechanical impacts, and protein denaturing. SFK may be activated by stresses directly or via regulatory circuits whose identity is not yet fully understood. Depending on the cell type and the nature of activating stimulus, SFK may activate known downstream signaling cascades leading to cell survival, proliferation, cytoskeletal rearrangement, and apoptosis; the identity of these cascades is discussed. As in the case of receptor-initiated signaling, roles of individual SFK in various stress response may be redundant or non-redundant. Although signals generated by different stresses are generally transduced via distinct SFK pathways, these pathways may overlap or exhibit crosstalk. In some cell types stress-induced activation of SFK promotes survival and inhibits apoptosis, whereas the opposite may be true for other cell types. Stress responses constitute a new and rapidly developing area of SFK-mediated signaling.