• Title/Summary/Keyword: stress development

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Development of Industrial Transgenic Plants Using Antioxidant Enzyme Genes (항산화효소 유전자를 이용한 산업용 형질전환식물체 개발)

  • LEE Haeng-Soon;KIM Kee-Yeun;KWON Suk-Yoon;KWAK Sang-Soo
    • Proceedings of the Korean Society of Plant Biotechnology Conference
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    • 2002.04a
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    • pp.49-58
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    • 2002
  • Oxidative stress derived from reactive oxygen species (ROS) is one of the major damaging factors in plants exposed to environmental stress. In order to develop the platform technology to solve the global food and environmental problems in the 21s1 century, we focus on the understanding of the antioxidative mechanism in plant cells, the development of oxidative stress-inducible antioxidant genes, and the development of transgenic plants with enhanced tolerance to stress. In this report, we describe our recent results on industrial transgenic plants by the gene manipulation of antioxidant enzymes. Transgenic tobacco plants expressing both superoxide dismutase (SOD) and ascorbate peroxidase (APX) in chloroplasts were developed and were evaluated their protection effects against stresses, suggesting that simultaneous overexpression of both SOD and APX in chloroplasts has synergistic effects to overcome the oxidative stress under unfavorable environments. Transgenic tobacco plants expressing a human dehydroascorbate reductase gene in chloroplasts were showed the protection against the oxidative stress in plants. Transgenic cucumber plants expressing high level of SOD in fruits were successfully generated to use the functional cosmetic purpose as a plant bioreactor. In addition, we developed a strong oxidative stress-inducible peroxidase promoter, SWPA2 from sweetpotato (Ipomoea batatas). We anticipate that SWPA2 promoter will be biotechnologically useful for the development of transgenic plants with enhanced tolerance to environmental stress and particularly transgenic cell lines engineered to produce key pharmaceutical proteins.

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Case Study on Reliability Prediction of Barrier Type Pulse Separation Device using Stress-Strength Analysis (부하-강도 분석을 이용한 격막형 펄스분리장치의 내열강도에 대한 신뢰성 예측 사례연구)

  • Lee, Dong-Won;Jeong, Se-Yong;Lee, Bang-Eop;Jung, Gyoo-Dong;Park, Boo-Hee;An, Dong-Geun;Jang, Joong-Soon
    • Journal of Applied Reliability
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    • v.15 no.2
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    • pp.124-130
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    • 2015
  • A stress-strength analysis is used to assess the reliability of a multi-pulse rocket motor system. Main stress is found to be thermal during explosion and the distribution is obtained by simulation. The strength distribution is derived from the results of actual specimen tests. The failure rate of barrier type pulse separation device is estimated.

The Sequential Mediation Effects of Warm Parenting Style and Daily Activity between Cumulative Parenting Stress and Child Development (어머니의 누적적인 양육스트레스와 아동의 인지 및 언어발달의 관계에서 온정적 양육방식과 일상적 상호작용의 매개효과)

  • Lee, Hyungmin
    • Korean Journal of Childcare and Education
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    • v.13 no.2
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    • pp.149-164
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    • 2017
  • Objective: This study aimed to examine the mediation effects of parenting style and daily activity on the relationships between cumulative parental stress and cognitive and language development of children. Methods: The participants of this study were 1,154 mothers, their five-year-old children, and teachers enrolled in the population-based study of the Panel Study of Korea Children (PSKC) conducted by the Korea Institute of Childcare and Education from 2008 to 2013. In order to analyze the data, descriptive analysis, Pearson correlation analysis, and structural equation modeling were used. Results: The results revealed that warm parenting style as well as daily activity had dual mediating effects between cumulative parental stress and child development (cognitive and language development). However, warm parenting style did not have any direct effects on child development in this model. Conclusion/Implications: Given that actual mother-child daily activity had an important role in affecting child development, support programs for parenting should include activities that include specific materials and activities which parents can do with their children in everyday life.

Isolation and Identification of Short Term Drought-Induced Genes in Zea mays L. Leaves

  • Rahman, Md. Atikur;Lee, Sang-Hoon;Choi, Gi Jun;Ji, Hee Jung;Kim, Won Ho;Lee, Ki-Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.3
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    • pp.237-241
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    • 2017
  • Drought is one of the detrimental factors that impair plant growth and productivity. In this study, we applied annealing control primer (ACP)-based reverse transcriptase PCR (polymerase chain reaction) technique to identify differentially expressed genes (DEGs) in maize leaves in response to drought stress. Two-week-old maize seedlings were exposed to drought (DT) by suspending water supply. DEGs were screened after 3 days of DT-treated samples using the ACP-based technique. Several DEGs encoding 16.9 protein, antimicrobial protein, hypothetical protein NCLIV_068840, thioredoxin M-type were identified in maize leaves under drought stress. These genes have putative functions in plant defense response, growth and development. These identified genes would be useful for predictive markers of plant defense, and growth responses under drought stress in plants.

Thermal Stress Analysis of Functuonally Graded Ceramic/Metal Composites(II) (경사기능성 세라믹/금속 복합재료의 열응력해석)

  • Lim, Jae-Kyoo;Song, Jun-Hee
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.21 no.10
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    • pp.1571-1579
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    • 1997
  • The development of a new material which should be continuously use under severe environment of very high temperature has been urgently requested. For the development of such super-heat resistant materials, the main problem is not only to make the superior thermal barrier properties but also to actively release thermal stress. So, a new concept of functionally graded material(FGM) has been proposed to overcome this problem. A composition and microstructure of FGM are varied continuously from place to place in ways designed to provide it with the maximum function of mitigating the induced thermal stress. So, FGM can be applied in the aerospace, the electronic and the medical field, etc.. In this study, thermal stress analysis of sintering PSZ/NiCrAlY graded material was conducted theoretically using a finite-element program. The temperature condition was sintering temperature assuming a cooling-down process up to room temperature. Fracture damage mechanism was anlayzed by the parameters of residual stress. It could be known that FGM provided with the function of mitigating the induced thermal stress.

Comparison of Gene Expression Changes in Three Wheat Varieties with Different Susceptibilities to Heat Stress Using RNA-Seq Analysis

  • Myoung Hui Lee;Kyeong-Min Kim;Wan-Gyu Sang;Chon-Sik Kang;Changhyun Choi
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.197-197
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    • 2022
  • Wheat is highly susceptible to heat stress, which significantly reduces grain yield. In this study, we used RNA-seq technology to analyze the transcript expression at three different time-points after heat treatment in three cultivars differing in their susceptibility to heat stress: Jopum, Keumkang, and Olgeuru. A total of 11,751, 8850, and 14,711; 10,959,7946, and 14,205; and 22,895,13,060, and 19,408 differentially-expressed genes (log2 fold-change > 1 and FDR (padj) < 0.05) were identified in Jopum, Keumkang, and Olgeuru in the control vs. 6-h, in the control vs. 12-h, and in the 6-h vs. 12-h heat treatment, respectively. Functional enrichment analysis showed that the biological processes for DEGs, such as the cellular response to heat and oxidative stress-and including the removal of superoxide radicals and the positive regulation of superoxide dismutase activity-were significantly enriched among the three comparisons in all three cultivars. Furthermore, we investigated the differential expression patterns of reactive oxygen species (ROS)-scavenging enzymes, heat shock proteins, and heat-stress transcription factors using qRT-PCR to confirm the differences in gene expression among the three varieties under heat stress. This study contributes to a better understanding of the wheat heat-stress response at the early growth stage and the varietal differences in heat tolerancea.

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A Study of Preschooler's Stress Coping Strategies Depending on Task and Temperament (과제 및 기질에 따른 유아의 스트레스 대처 전략)

  • Kim Jimin;Yoo An Jin
    • Journal of the Korean Home Economics Association
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    • v.43 no.1 s.203
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    • pp.177-189
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    • 2005
  • This study investigated 3- and 5-year-old preschoolers' stress coping strategies depending on tasks, and whether preschoolers' stress coping strategies in each task depended on their temperament types. Subjects were 96 3- and 5-year-old preschoolers selected from three day-care centers in Seoul, Kounggi. A Parent Temperament Questionnaire for preschooler was used, and the coping strategies which each preschooler showed in task with perceived controllable, task with perceived uncontrollable were recorded by a video camera. Preschoolers' stress coping strategies recorded by a video camera were transcribed and analyzed according to the categories the researcher generated. Data were analyzed by paired t-tests and t-tests. As a results, there was a significant differences in preschoolers' stress coping strategies depending on the tasks and a significant temperament type difference in the preschoolers' stress coping strategies in each task.

Methodology for Centrifugal Stress Estimation Model Development of Large Steam Turbine Blades (스팀 터빈 블레이드 원심응력 추정을 위한 전산해석 연구)

  • Lee, Byounghak;Park, Jongho
    • The KSFM Journal of Fluid Machinery
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    • v.16 no.6
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    • pp.26-31
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    • 2013
  • Last blades of low-pressure turbine in nuclear power plant are the highly damaged part and always suffered from different types of loadings leading to various stress components, stresses due to centrifugal force and steam flow loading. Especially, centrifugal stress generated by turbine rotation is one of the main problems and more significant than other stresses as they have the greatest effect on total stress. Therefore, this study was performed to obtain the important information for estimation model development of the blade centrifugal stress level and distribution.

Toddler's Gender and Temperament by Maternal Stress as Predictors of Mothers' Parenting Behavior (걸음마기 아동의 성, 기질 및 어머니의 스트레스가 양육행동에 미치는 영향)

  • Park, Seong Yeon;Rubin, Kenneth H.
    • Korean Journal of Child Studies
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    • v.29 no.2
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    • pp.109-124
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    • 2008
  • The objective of this study was to identify how child's gender, temperament, mother's stress and their interactions predict parenting behavior. The subjects were 97 2-year-olds(47boys; 50girls) and their mothers. Data were gathered by questionnaires. Results of a series of hierarchical regression analyses showed combinations of variables that predicted parenting behaviors : (1) mothers reported higher over-protectiveness toward girls and when they were under higher stress. (2) An interaction effect showed that mothers with sons who had higher 'difficult' temperaments reported greater over-protective parenting under greater stress. (3) There were no significant differences in over-protective parenting under low stress, regardless of the level of difficult temperament. (4) Mother's stress was a strong predictor of supportive parenting behaviors.

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The Relationship Among Nurses' Parenting Stress and Turnover Intention (간호사의 양육스트레스와 이직의도의 관계)

  • Kang, Su-Jung;Kim, Souk-Young
    • Journal of Korean Academy of Nursing Administration
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    • v.17 no.4
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    • pp.508-515
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    • 2011
  • Purpose: The purposes of this study were to understand correlation of parenting stress and turnover intention of nurses, and to provide basic data for effective nursing management. Methods: Subjects included 287 nurses who had children and worked in hospitals. Data were collected through organized questionnaires from September 1st to 30th 2010. Collected data was analyzed with descriptive statistics, t-test, ANOVA, correlation analysis using PASW statistics 18 program. Results: The average score for parenting stress of the participants was 3.12 out of 5. Nurture stress was statistically different depending on age, job position, shift work, night duty and stages of family development. The average score for turnover intention of the participants was 3.34 out of 5. Turnover intention was statistically different depending on age, number of children and stages of family development. As a result of correlation analysis of parenting stress and turnover intention, turnover intention showed positive correlation with daily stress, stress caused from taking parent role and average parenting stress. Conclusion: This study indicates that parenting stress is related with turnover intention, nursing managers should make efforts to decrease parenting stress in order to reduce turnover intention of nurses.