• Journal of the Korean Society of Propulsion Engineers
• /
• v.5 no.1
• /
• pp.76-81
• /
• 2001

A structural test of the wind turbine rotor blade is to evaluate the uncertainty of design due to selection of material, design concepts, production processes and so on, and their possible impacts on the structural integrity. In the full-scale static strength test, the measuring parameters are strain and displacements vs. loads, weight and the center of gravity. In order to simulate the aerodynamics load, the three-point loading method is applied. There is slight difference between the measured results and the predicted results for the reference fiber volume fraction of 60% . However, the agreement between the measured results and the predicted results with the actual fiber volume fraction of 52.5% is good. Even though a slightly non-linearity from 80% loading to 100% loading exists, a linear static solution is sufficient for the design purpose due to te small amount of non-linearity. Comparison between measured and predicted strain results at the maximum thickness positions of the blade profile for 0.236R(5.56m), 0.493R(11.59m) and 0.574R(13.43m), under 20%, 40%, 60%, 80% and 100% loadings for the upper part of the blade. The predicted values are in good agreement with the measured values.

• Microbiology and Biotechnology Letters
• /
• v.46 no.4
• /
• pp.334-345
• /
• 2018

Spore-forming Bacillus species are commercially available probiotic formulations for application in humans. They have health benefits and help prevent disease in hosts by combating entero-pathogens and ameliorating antibiotic-associated diarrhea. However, the molecular and cellular mechanisms of these benefits remain unclear. Here, we report the draft genome of a potential probiotic strain of Bacillus clausii B106. We mapped and compared the probiotic profile of B106 with other reference genomes. The draft genome analysis of B106 revealed the presence of ADI pathway genes, indicating its ability to tolerate acidic pH and bile salts. Genes encoding fibronectin binding proteins, enolase, as well as a gene cluster involved in the biosynthesis of exopolysaccharides underscored the potential of B106 to adhere to the intestinal epithelium and colonize the human gut. Genes encoding bacteriocins were also detected, indicating the antimicrobial ability of this isolate. The presence of genes encoding vitamins, including Riboflavin, Folate, and Biotin, also indicated the health-promoting ability of B106. Resistance of B106 to multiple antibiotics was evident from the presence of genes encoding resistance to chloramphenicol, ${\beta}$-lactams, Vancomycin, Tetracycline, fluoroquinolones, and aminoglycosides. The findings indicate the significance of B. clausii B106 administration during antibiotic treatment and its potential value as a probiotic strain to replenish the health-promoting and disease-preventing gut flora following antibiotic treatment.

• Geomechanics and Engineering
• /
• v.35 no.1
• /
• pp.1-11
• /
• 2023

A growing trend of utilizing helical piles for soft soil strata to support infrastructure projects is currently observed in Saemangeum, South Korea. Recognized mainly due to its ease of installation and reusability proves to be far more superior compared to other foundation types in terms of sustainability. This study applies modified p-y springs to characterize the behavior of a laterally loaded helical pile with a shaft diameter of 89.1 mm affixed with 3 helices evenly spaced along its embedded length of 2.5 m. Geotechnical soil properties are correlated from CPT data near the test bed vicinity and strain gauges mounted on the shaft surface. A modification factor is applied on the p-y springs to adjust the simulated data and match it to the bending moment, soil resistance and deflection values from the strain gauge measurements. The predicted lateral behavior of the helical pile through the numerical analysis method shows fairly good agreement to the recorded field test results.

• Journal of Mushroom
• /
• v.14 no.2
• /
• pp.59-63
• /
• 2016

A new commercial strain of oyster mushroom (was developed by hyphal anastomosis, and was improved byhybridization between a monokaryotic strain derived from Pleurotus ostreatus ASI 0635 (Gonji 7ho) and a dikaryotic strain derived from P. ostreatus ASI 0666 (Mongdol). The optimum temperatures for mycelial growth and fruiting body development were $25{\sim}30^{\circ}C$ and $12{\sim}18^{\circ}C$, respectively. When PDA (potato dextrose agar medium) and MCM (mushroom complete medium) were compared, mycelial growth was faster in MCM. Similar results were observed with the control strain P. ostreatus ASI 2504 (Suhan 1ho). Analysis of the genetic characteristics of the new cultivar ('Gosol') showed a different DNA profile from that of the control ASI 2504 strain, when RAPD (raurpDNA) primers URP1, 2, 3, and 7 were used. Fruiting body production per bottle was approximately116 g based on a production performance test. In addition, yields from a farm field trial were stably achieved in an inadequate production enviro. The color of the pileus was blackish gray, and the stipe was long and thick. Therefore, we expect that this new strain will satisfy consumer demand for high quality mushrooms.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.30 no.2
• /
• pp.46-51
• /
• 2002

Cold expansion of fastener holes is a mechanical process widely used in the aerospace industry. This treatment leads to an improvement of fatigue behavior due to the developed compressive residual stresses on the hole surface. The residual stress profile depends on the parameters of cold expansion, which are expanding rate, inserting direction of mandrel, material properties dtc. Despite its importance to aerospace industiries, little attention has been devoted to the accurate modeling of the process. In this paper, three-dimensional finite element simulations have been conducted for the cold expansion in an aluminium plate in order to predict the magnitude and distribution of the residual stress. To prove the results of FE analysis, the residual strain was measured by strain gage in cold expansion test. Maximum compressive residual stress could be increase about 7 percentage using the 2-step cold expansion method.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.11 no.1
• /
• pp.57-61
• /
• 2005

A strain of Bacillus thuringiensis that showed signifi­cantly high toxicity to Plutella xylostella was isolated from a dust sample collected from Chinese tobacco warehouse and characterized. The isolate named B. thuringiensis LY-99 was determined to belong to subsp. alesti (H3a3c) by an H antisera agglutination test and produced bipyramidal inclusions. Plasmid and crystal protein patterns of the LY-99 were different from those of the reference strain, subsp. alesti. PCR analysis with specific primers revealed that this isolate contained abundant cry genes including crylAa, crylAc, crylB, crylD, crylE, crylF and cry2 genes, which was absolutely different from cry gene profile of the subsp. alesti. In addition, insecticidal activity of the LY-99 against P. xylostella larvae was about 44 times higher than that of the subsp. alesti.

• Journal of the Korean Institute of Gas
• /
• v.12 no.4
• /
• pp.34-40
• /
• 2008

Using the finite element method and Taguchi's design technique, the displacement in vertical direction, von Mises stress, and strain energy of the corrugation damper have been analyzed as functions of the extruded length and the thickness of the corrugation damper, and the upper and lower corner radii of the damper. The optimized profile design elements of a corrugation damper are very important for increasing a strain energy absorption capacity of a helmet structure, which is attacked by impulsive external forces. In this study, the optimized design data based on the Taguchi's method was computed as a corrugation damper length of L = 20 mm, a damper thickness of t = 2 mm, the upper corner radius of $R_1=4\;mm$, and the lower corner radius of $R_2=3\;mm$. The optimized design parameters of a corrugation damper indicated that the thickness and extruded length of a corrugation damper may affect to increase the strain energy, which absorbs the impact forces of the helmet.

• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.9
• /
• pp.1336-1342
• /
• 2005

The present research work was conducted to evaluate the beneficial effects as well as the safety aspects of lactobacilli as probiotic. Lactobacilli were isolated from poultry faecal samples, feed samples and from some known preparations procured from poultry feed manufacturers. L. acidophilus and L. sporogenes were tested for the antibacterial activity against four poultry pathogens viz. Escherichia coli, Salmonella spp., Proteus spp. and Pseudomonas aeruginosa. Cell free supernatant (CFS) of L. acidophilus exhibited significantly higher antibacterial activity against Salmonella spp. at original pH (4.50${\pm}$0.02). At the adjusted pH (6.50${\pm}$0.02) significantly higher antibacterial activity was recorded against indicator organism except for P. aeruginosa. Likewise, L. sporogenes exhibited similar antibacterial activity at original as well as adjusted pH except for E. coli. Antibacterial activity against E. coli was significantly higher at adjusted pH than at original pH of CFS. The competitive exclusion of E. coli by lactobacilli over the intestinal epithelial cells (IEC) was checked. L. acidophilus strain I, which was of poultry origin, exhibited maximum attachment over IEC as compared to other three strains of non-poultry origin viz. L. acidophilus strain II, L. sporogenes strain I and II. Overall, L. acidophilus exhibited higher competitive exclusion as compared to L. sporogenes. All the lactobacilli of poultry origin were most sensitive to penicillin G, amoxycillin, ampicillin and chloramphenicol, least sensitive to sulphamethizole, ciprofloxacin, neomycin, norfloxacin and pefloxacin and resistant to metronidazole and nalidixic acid. The isolates from probiotic preparations were most sensitive to ampicillin, amoxycillin and tetracycline, least sensitive to sulphamethizole, norfloxacin, neomycin and ceftriazone and resistant to nalidixic acid and metronidazole. Eight of the multiple drug resistant lactobacilli isolates were studied for the presence of plasmids. Plasmids could be extracted from six isolates of lactobacilli. These plasmids could be responsible for bacteriocin production or for antibiotic resistance of the strains. The lactobacilli need further studies regarding their safety for use in the probiotic preparations.

• KSBB Journal
• /
• v.31 no.4
• /
• pp.263-269
• /
• 2016

For decades, the microorganisms in arctic soils have been newly discovered according to the climate change and global warming. In this study, the chemical structure of a lipid A molecule from Pseudomonas sp. strain PAMC 28615 which was newly discovered from arctic soils was characterized by mass spectrometric approaches such as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and MALDI multi-stage tandem mass spectrometry (MS). First, lipopolysaccharide (LPS) from Pseudomonas sp. strain PAMC 28615 was extracted and subsequently hydrolyzed to obtain the lipid A. The parent ion peak at m/z 1632 was determined by MALDI-TOF MS, which also can validate our lipid A purification method. For detailed structural determination, we performed the multiple-stage tandem mass analysis ($MS^4$) of the parent ion, and subsequently the abundant fragment ions in each MS stage are tested. The fragment ions in each MS stage were produced from the loss of phosphate groups and fatty acyl groups, which could be used to confirm the composition or the position of the lipid A components. Consequently, the mass spectrometry-based lipid A profiling method could provide the detail chemical structure of lipid A from the Pseudomonas sp. strain PAMC 28615 as an arctic bacterium from the frozen arctic soil.

• Journal of Microbiology and Biotechnology
• /
• v.23 no.4
• /
• pp.459-466
• /
• 2013

The lactic acid bacterium Streptococcus thermophilus is widely used as a starter culture for the production of dairy products. Whole-genome sequencing is expected to utilize the genetic basis behind the metabolic functioning of lactic acid bacterium (LAB), for development of their use in biotechnological and probiotic applications. We sequenced the whole genome of Streptococcus thermophilus MTCC 5461, the strain isolated from a curd source, by 454 GS-FLX titanium and Ion Torrent PGM. We performed comparative genome analysis using the local BLAST and RDP for 16S rDNA comparison and by the RAST server for functional comparison against the published genome sequence of Streptococcus thermophilus CNRZ 1066. The whole genome size of S. thermophilus MTCC 5461 is of 1.73Mb size with a GC content of 39.3%. Streptococcal virulence-related genes are either inactivated or absent in the strain. The genome possesses coding sequences for features important for a probiotic organism such as adhesion, acid tolerance, bacteriocin production, and lactose utilization, which was found to be conserved among the strains MTCC 5461 and CNRZ 1066. Biochemical analysis revealed the utilization of 17 sugars by the bacterium, where the presence of genes encoding enzymes involved in metabolism for 16 of these 17 sugars were confirmed in the genome. This study supports the facts that the strain MTCC 5461 is nonpathogenic and harbors essential features that can be exploited for its probiotic potential.