International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Molecular Characterization of A Novel Bacillus thuringiensis Strain from China

  • Qi Xu Feng (College of Plant Science and Technology, Huazhong Agricultural University) ;
  • Li Ming Shun (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Choi Jae Young (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Kim Yang-Su (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Wang Yong (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Kang Joong Nam (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Choi Heekyu (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Je Yeon Ho (School of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University) ;
  • Song Ji Zhen (Zhengzhou Tobacco Research Institute, China National Tobacco Corporation) ;
  • Li Jian Hong (College of Plant Science and Technology, Huazhong Agricultural University)
  • Published : 2005.09.01
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Abstract

A strain of Bacillus thuringiensis that showed signifi­cantly high toxicity to Plutella xylostella was isolated from a dust sample collected from Chinese tobacco warehouse and characterized. The isolate named B. thuringiensis LY-99 was determined to belong to subsp. alesti (H3a3c) by an H antisera agglutination test and produced bipyramidal inclusions. Plasmid and crystal protein patterns of the LY-99 were different from those of the reference strain, subsp. alesti. PCR analysis with specific primers revealed that this isolate contained abundant cry genes including crylAa, crylAc, crylB, crylD, crylE, crylF and cry2 genes, which was absolutely different from cry gene profile of the subsp. alesti. In addition, insecticidal activity of the LY-99 against P. xylostella larvae was about 44 times higher than that of the subsp. alesti.

Keywords

References

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