International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지

Studies on the Hereditary Properties of SSR Marker in Silkworm (Bombyx mori L.)

  • Li Muwang (Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Graduate School of the Chinese Academy of Sciences) ;
  • Li Minghui (Graduate School of the Chinese Academy of Sciences) ;
  • Miao Xuexia (Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences) ;
  • Lu Cheng (Southwest Agriculture University) ;
  • Huang Yongping (Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences)
  • Published : 2005.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Two BC1 group, c108 $\times$ (p50 $\times$ c108) and p50 $\times$ (p50 $\times$ c108), one group of F$_{2}$ progeny, (p50 $\times$ c108) F$_{2}$ ,and 3 SSR markers, F10539, FlO626 and FlO618 were used to test the hereditary properties of SSR markers in silkworm. FI0539, FlO626 were proved to be linkage, and FlO618 was proved to be independent to those two markers. According to Mendel's law, the recombinant value between F10539, FlO626 was calculated in all of these groups, and they were 8.55$\%$ (c108BC1), 8.02$\%$ (p50BC1) and 7.81 $\%$ (F$_{2}$) respectively. There was dominant difference among the crossing-over value using paired-samples tests by SPSS 10.0 software. This research proved that SSR markers were co-dominant in B. mori too, and F 2 progeny could be used to construct SSR linkage map although B. mori lacked of crossing over in females.

Keywords

References

  1. Abe, H., T. Sugasaki and M. Kanehara (2000) Identification and genetic mapping of RAPD markers linked to the den sonucleosis refractoriness gene, nsd-2, in the silkworm, Bombyx mori. Genes Genet. Syst. 75, 93-96 https://doi.org/10.1266/ggs.75.93
  2. Botstein, D., R. L. White, M. Skolnick and R. W. Davis (1980) Construction of genetic linkage map in man using restriction length polymorphisms. Am. J Hum. Genet. 32,3 14-331
  3. Chatterjee, S. N. and T. P. Mohandas (2003) Identification of ISSR markers associated with productivity traits in silkworm, Bombyx mori L. Genome 46, 438-447 https://doi.org/10.1139/g03-024
  4. Edwards, A., A. Civitello, H. A. Mmond and C. T. Caskey (1991) DNA typing and genetic mapping with trim eric and tetrameric tandem repeats. Am. J Hum. Genet. 49,746-756
  5. Goldsmith, M. R., T. Shimada and H. Abe (2005) The genetics and genomics of the silkworm, Bombyx mori. Annu. Rev. Entomol. 50, 71-100 https://doi.org/10.1146/annurev.ento.50.071803.130456
  6. Goldstein, D. and A. G. Clark (1995) Microsatellite variation in North American populations of Drosophila melanogaster. Nucleic Acids Res. 7, 3882-3886
  7. He, N. J., C. Lu, B. Li, Z. Y. Zhou and Z. H. Xiang (2001) The construction of linkage map of Bombyx mori by combination of SADF and RAPD. Acta Entomologia Sinica 44, 476-482
  8. Huang, W G., G. Cipriani, M. Morgante and R. Testolin (1998) Microsatellite DNA in Actinidia chinensis: isolation, characterisation, and homology in related species. Theor. Appl. Genet. 97, 1269-1278 https://doi.org/10.1007/s001220051019
  9. Li, M. W, Q. Yao, C. X. Hou, C. Lu and K. P. Chen (2001) Studies on RAPD markers linked the densonucleosis reeractoriness gene, nsd-Z in silkworm, Bombyx mori L. Sericologia 41, 409-415
  10. Li, M. W, L. Shen, A. Y. Xu, X. X. Miao, C. X. Hou, P. J. Sun, Y. H. Zhang and Y. P. Huang (2005) Genetic diversity among the silkworm (Bombyx mori L., Lep., Bombycidae) germplasm revealed by microsatellites. Genome In press
  11. Lu, C., B. Li, A. Zhao and Z. Xiang (2004) QTL mapping of economically important traits in Silkworm (Bombyx mori). Science in China Series C-Life Sciences 47, 477-484 https://doi.org/10.1360/03yc0260
  12. Morgante, M. and A. M. Olivieri (1993) PCR-amplified microsatellites as markers in plant genetics. Plant J 3, 175-182 https://doi.org/10.1111/j.1365-313X.1993.tb00020.x
  13. Prasad, M. D., M. Muthulakshmi, M. Madhu, S. Archak, K. Mita and J. Nagaraju (2005) Survey and analysis of microsatellites in the silkworm, Bombyx mori: Frequency, distribution, mutations, marker potential and their conservation in heterologous species. Genetics 169, 197-214 https://doi.org/10.1534/genetics.104.031005
  14. Promboon, A., T. Shimada, H. Fujiwara and M. Kobayashi (1995) Linkage map of RAPDs in the silkworm, Bombyx mori. Genet. Res. Camb. 66, 1-7 https://doi.org/10.1017/S0016672300034339
  15. Reddy, K. D., E. G. Abraham and J. Nagaraju (1999) Microsatellites in the silkworm, Bombyx mori: abundance, polymorphism, and strain characterization, Genome 42,1057-1065 https://doi.org/10.1139/gen-42-6-1057
  16. Shi, J., D. G. Heckel and M. R. Goldsmith (1995) A genetic linkage map for the domesticated silkworm, Bombyx mori, based on restriction fragment length polymorphisms, Genet. Res. 66, 109-126 https://doi.org/10.1017/S0016672300034467
  17. Tan, Y. D., C. L. Wan, Y. F. Zhu, C. Lu, Z. H. Xiang and H. W Deng (2001) An amplified fragment length polymorphism map of the silkworm. Genetics 157, 1277-1284
  18. Tautz, D. (1989) Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucleic Acids Res. 17,6463-6471 https://doi.org/10.1093/nar/17.16.6463
  19. Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. Van de Lee, M. Homes, A. Frijters, J. Pot, J. Peleman, M. Kuiper and M. Zabeau (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23,4407-4414 https://doi.org/10.1093/nar/23.21.4407
  20. Williams, J. G. K., M. K. Hanafey, J. A. Rafalsk and S. V. Tingey (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531-6535 https://doi.org/10.1093/nar/18.22.6531
  21. Xiang, D. Q., H. H. Cao, Y. G. Cao, J. P. Yang, L. J. Huang, S. C. Wang and J. R. Dai (2001) Construction of a genetic map and location of quantitative trait loci for yield component traits in maize by SSR markers. Acta Genetica Sinica 28, 778-784
  22. Yao, Q., M. W. Li, Y. Wang, W. B. Wang, J. Lu, Y. Dong and K. P. Chen (2003) Screening of molecular markers for NPY resistance in Bombyx mori L. (Lep.: Bombycidae). J. Appl. Ent. 127,134-136 https://doi.org/10.1046/j.1439-0418.2003.00741.x
  23. Yasukochi, Y. (1998) A dense genetic linkage map of the silkworm, Bombyx mori, covering all chromosomes based on 1018 molecular markers. Genetics 150, 1513-1525
  24. Yu, P., Z. C. Li, H. L. Zhang, D. Y. Li, M. X. Wang, J. L. Sun and X. K. Wang. (2004) Genetic diversity of common wild rice (Oryza rufipogon Grift) by using SSR markers and Phenotypic traits in Guangxi province. Acta Genetica Sinica 31, 934-940
  25. Zhao, A. C., C. Lu, B Li, X. Y. Pu, Z. Y. Zhou and Z. H. Xiang (2004) Construction of AFLP molecular markers linkage map and localization of green cocoon gene in silkworm (Bambyx morii. Acta Genetica Sinica 31, 787-794