• Title/Summary/Keyword: stomatal characteristics

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Growth and Physiological Response of Three Evergreen Shrubs to De-icing Salt(CaCl2) at Different Concentrations in Winter - Focusing on Euonymus japonica, Rhodoendron indicum, and Buxus koreana - (겨울철 염화칼슘(CaCl2) 처리에 따른 가로변 3가지 상록 관목류의 생육 및 생리반응 - 사철나무, 영산홍, 회양목을 중심으로 -)

  • Ju, Jin-Hee;Park, Ji-Yeon;Xu, Hui;Lee, Eun-Yeob;Hyun, Kyoung-Hak;Jung, Jong-Suk;Choi, Eun-Young;Yoon, Yong-Han
    • Journal of the Korean Institute of Landscape Architecture
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    • v.44 no.2
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    • pp.122-129
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    • 2016
  • It is important to know the sensitivity of shrubs to de-icing salt in order to set guidelines for ecological tolerance of evergreen shrubs along roads. Therefore, the aim of this study was to investigate the influence of de-icing salt, calcium chloride($CaCl_2$), on the growth and physiological characteristics of three evergreen shrubs, Euonymus japonica, Rhododendron indicum, and Buxus koreana. Plants were exposed to calcium chloride at different concentrations(weight percentage, 0% as control, 1.0%, 3.0%, and 5.0%) through amended soil maintained from the start of the experiment in October of 2014 until termination in March of 2015. The survival rate, plant height, leaf length, leaf width, leaf shape index, number of leaves, fresh weight, dry weight, dry matter, root/top ratio, chlorophyll contents, fluorescence, photosynthesis, stomatal conduct, and transpiration rate were recorded. Elevated calcium chloride concentrations decreased plant height, leaf length, leaf width, leaf shape index, fresh weight, dry weight, dry matter, and R/T ratio of the three shrubs. Root growth responded more sensitively than the top growth to salinity. However Euonymus japonica was more tolerant to salt stress than Rhododendron indicum and Buxus koreana. Their growths were totally inhibited by $CaCl_2$ above 3.0% and 1.0% concentrations, respectively. Chlorophyll content, fluorescence, photosynthesis, stomatal conduct, and transpiration rate of both Rhododendron indicum and Buxus koreana were reduced sharply, while Euonymus japonica exhibited mild reductions compared to plants grown in control when increasing calcium chloride was used. Especially, the transpiration rates of Rhododendron indicum, and the photosynthesis and stomatal conduct of Buxus koreana were suppressed as the concentrations of calcium chloride increased. Therefore, Euonymus japonica should be considered as an ecologically tolerant species with proven tolerance to de-icing salt.

Growth Characteristics and Photosynthesis of Soybean Seedling to NaCl stress in Sand Culture (콩의 유묘기에 있어서 NaCl Stress에 의한 생육특성과 광합성 반응)

  • Cho, Jin-Woong;Kim, Choong-Soo
    • Korean Journal of Environmental Agriculture
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    • v.18 no.4
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    • pp.361-365
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    • 1999
  • This atudy was conducted to determine the growth characteristics and photosynthesis of soybean (Glycine max L. cv. Keumjongkongl) 30 day old seedling to 100mM NaCl concentration containing 1/2 Hoagland`s nutrient solution in sand culture. The nodule formation of root is not found perfectly with NaCl stress. The leaf dry matter weight (g/plant) of stressed plant is more reduction in 77% to control than any other characters. The water content (%) is tend to increase but water potential (MPa) is tend to decrease at NaCl stress. The chlorophyll content (SPAD) is tend to increase at growing leaf age of control but decrease at NaCl stress. The photosynthesis, stomatal conductance and transpiration are tend to decrease sharply at NaCl stress.

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Environmental controls on growing-season sap flow density of Quercus serrata Thunb in a temperate deciduous forest of Korea

  • Laiju, Nahida;Otieno, Dennis;Jung, Eun-Young;Lee, Bo-Ra;Tenhunen, John;Lim, Jong-Hwan;Sung, Joo-Han;Kang, Sin-Kyu
    • Journal of Ecology and Environment
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    • v.35 no.3
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    • pp.213-225
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    • 2012
  • Sap flux density (SFD) measurements were used, in combination with morphological characteristics of trees and forest structure, to calculate whole-tree transpiration, stand transpiration (St) and mean canopy stomatal conductance (Gs). Analysis based on the relationships between the morphological characteristics of trees and whole tree water use, and on the responses of SFD and Gs to short wave radiation (RR), vapor pressure deficit (VPD) and soil water content (SWC) during drought and non-drought periods were conducted. The results showed a strong positive correlation between whole tree transpiration and both tree diameter at breast height (DBH) ($r^2$ = 0.95, P < 0.05) and sapwood area (SA) ($r^2$ = 0.98, P < 0.05). Relationships between SFD and DBH ($r^2$ = 0.25), as well as SA ($r^2$ = 0.17) were weak. Daily SFD of Quercus serrata Thunb was closely related to VPD and RR. Although operating at different time scales, RR and VPD were important interacting environmental controls of tree water use. SFD increased with increasing VPD (<1 kPa) and RR. SWC had a considerable effect on stand transpiration during the drought period. The relationships between SFD, VPD and RR were distorted when SWC dropped below 35%.

Anatomical Characteristics of Hyperhydric Shoots Occuring in In Vitro Culture of Peace Poplar (Peace포플러의 기내 배양시 발생하는 과수화 식물체의 조직적 특성)

  • Kang, Hyo-Jin;Moon, Heung-Kyu;Park, So-Young;Kim, Pan-Gi
    • Journal of Plant Biotechnology
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    • v.31 no.2
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    • pp.145-149
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    • 2004
  • We investigated the anatomical aspects of vitrification in peace poplar. Comparisons were made with regard to characteristics occurring between hyperhydric and normal shoots in shoot proliferation cultures on MS medium containing 0.2 mg/L BA. Compared with normal plants, hyperhydric plants had thick, curled, and dark green leaves. Hyperhydric stems were thicker and shorter than those of normal stems. When examined under the microscopes, the mesophyll palisade cells of hyperhydric leaves were vacuolated, whereas those of normal leaves contained normal and enriched vacuole with cytoplasm. Generally, the hyperhydric leaves showed poorly developed palisade parenchyma, and revealed irregular and bigger sized intercellular structures in both palisade and spongy parenchyma as well as epidermis cells compare to those of normal leaves. In addition, the hyperhydric leaves had lower stomatal density and bigger sized cell. Vascular tissues of hyperhydic stems were less differentiated because of poorly lignified xylem tissue. The greatly expanded cortical cells and pith appeared to be the main cause of thick stems as compared with normal stems.

Evaluation of Growth Characteristics and Groundwater Levels for the Growth and Development of Sorghum (Sorghum bicolor L.) and Adzuki bean(Vigna anaularis L.)

  • Ryu, Hee-La;Adhikari, Arjun;Kang, Sang-Mo;Kim, Yoon-Ha;Lee, In-Jung
    • Journal of agriculture & life science
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    • v.52 no.6
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    • pp.13-25
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    • 2018
  • Appropriate water level is the primary factor for the optimal yield of crop plants. The required water level varies according to the variety of the crops. In the present study, we investigated the optimum requirement of groundwater level(GWL) to grow sorghum and adzuki bean under paddy field soil. Here, we cultivated sorghum and adzuki bean using lysimeter filled with paddy soil under GWL 0 cm(NT) and GWL(20, 40 cm) where GWL 20 cm is maintained as a waterlogging condition. The plant growth promoting attributes were measured on the first day after treatment(0 DAT), 10 DAT and 20 DAT. The results showed that the growth parameter such as shoot length, leaf length, leaf width, and stem thickness of both sorghum and adzuki bean were constantly increased and were found higher at GWL 40 cm(except stem thickness and leaf width in sorghum at 20 DAT). The physiological parameters such as chlorophyll content and stomatal conductance were also found higher at GWL 40 cm in all DAT. In addition, the elements like P and K contents in adzuki bean, and Ca content in sorghum were constantly increased and was found higher in GWL 40 cm at all DAT. These results suggest that the GWL of 40 cm is appropriate for production of sorghum and adzuki bean especially in case of paddy soil.

Studies on Cytological Characteristics of Elymus humidus as Genetic Resources Compared to Common Wheat (Triticum aestivum L.)

  • Ji-Yoon Han;Seong-Wook Kang;Sejin Oh;Yumi Lee;Myoung-Jae Shin;Sukyeung Lee;Seong-Woo Cho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.3
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    • pp.216-223
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    • 2023
  • This study aimed to identify and compare the characteristics of Elymus humidus on common wheat (Triticum aestivum L. cv. Chinse Spring, CS). The seed length, width, height, and weight of E. humidus were smaller than those of the CS. In particular, the germination rate of E. humidus was substantially lower than that of CS. In the anatomical dissection of the leaf, E. humidus showed a considerably different xylem diameter of the main vascular bundle in the main vein; however, there was no difference in the phloem of the main vascular bundle compared with the xylem and phloem of the main vascular bundle in the main vein of CS, although E. humidus showed a leaf structure similar to that of CS. In addition, E. humidus had a thinner epidermis than that of CS. Regarding stomatal traits, E. humidus showed a graminoid stomata type similar to that of CS. On the adaxial and abaxial sides, the density, length, and width of the stomata in E. humidus were smaller than those in CS, whereas the distance between stomata in E. humidus was greater than that in CS. The chromosomes of E. humidus were classified as long and short based on their respective lengths. Long chromosomes were classified based on the ratio of the long arm to the short arm e.g., 1:1 or 2:1. Short chromosomes showed the same trend and some short chromosomes were microsatellites. To evaluate genetic diversity, 38 barley EST markers with polymorphisms between E. humidus and CS were selected from 236 barley EST markers.

Misconceptions and Truths of Morphological Characteristics in Plant Stomata (식물에서 기공 형태에 대한 오해와 진실)

  • Kim, Dae Jae;Lee, Joon Sang
    • Journal of Life Science
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    • v.27 no.2
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    • pp.241-246
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    • 2017
  • The walls of guard cells have many different specialized features. Guard cells are present in leaves of bryophytes, ferns and almost all of the vascular plants. Guard cells show considerable morphological diversities. It is understood that the stomata show two types in terms of morphological characterizations of guard cells. The first type is only found in a few monocots including Poaceae and Cyperaceae. In rice and corn, guard cells have the morphological characteristics of dumbbell shape. The morphological characteristics of dumbbell shape always have subsidiary cells. The other type is found in every dicots and many monocots and they are kidney-shaped guard cells. The plants of kidney-shaped guard cells rarely have subsidiary cells except Commelina communis L. Therefore, it could be concluded that two types of the morphological characteristics of guard cells cannot divide according to monocots or dicots. Every plants in which stomatal characteristic features were all different, most of them belong to kidney-shaped guard cells. However in case of Sedum sarmentosum, guard cells were shown to be long and narrow lips type. In Tradescantia virginiana, the shape of guard cells could be called perfectly to half-moon type. Therefore, it could be concluded that kidney-shaped types are all different in some way, but dumbbell-shaped types are almost constant.

The effect on photosynthesis and osmotic regulation in Beta vulgaris L. var. Flavescens DC. by salt stress

  • Choi, Deok-Gyun;Hwang, Jeong-Sook;Choi, Sung-Chul;Lim, Sung-Hwan;Kim, Jong-Guk;Choo, Yeon-Sik
    • Journal of Ecology and Environment
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    • v.39 no.1
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    • pp.81-90
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    • 2016
  • This study was to investigate the effect of salt stress on physiological characteristics such as plant growth, photosynthesis, solutes related to osmoregulation of Beta vulgaris. A significant increase of dry weight was observed in 50 mM and 100 mM NaCl. The contents of Chl a, b and carotenoid were lower in NaCl treatments than the control. On 14 day after NaCl treatment, photosynthetic rate (PN), the transpiration rate (E) and stomatal conductance of CO2 (gs) were reduced by NaCl treatment. On 28 day after NaCl treatment, the significant reduction in gs and E was shown in NaCl 200 mM. However, PN and water use efficiency (WUE) in all NaCl treatments showed higher value than that of control. Total ion contents (TIC) and osmolality were higher than the control. On 14 day after treatment, the contents of proline (Pro) increased significantly in 200 mM and 300 mM NaCl concentration compared with control, whereas on 28 day in all treatments it was lower than that of the control. The contents of glycine betaine (GB) increased with the increase of NaCl concentration. The contents of Na+, Cl-, GB, osmolality and TIC increased with the increase of NaCl concentrations. These results suggested that under severe NaCl stress conditions, NaCl treatment did not induce photochemical inhibition on fluorescence in the leaves of B. vulgaris, but the reduction of chlorophyll contents was related in a decrease in leaf production. Furthermore, increased GB as well as Na+ and Cl- contents resulted in a increase of osmolality, which can help to overcome NaCl stress.

Antioxidant Enzyme Activities of Alnus firma to Air Pollution in Yochon Industrial Complex (여천산업단지(麗川産業團地) 사방오리나무의 공해(公害) 방어(防禦) 기작(機作)에 관여(關與)하는 효소(酵素)들의 활성비교(活性比較))

  • Woo, Su-Young;Lee, Don Koo
    • Journal of Korean Society of Forest Science
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    • v.88 no.2
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    • pp.221-228
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    • 1999
  • This study measured the seasonal changes in physiological characteristics and antioxidants of Alnus firma to compare several enzyme activities(Rubisco, Superoxide dismutase(SOD) and Glutathione Reductase(GR)) between resistant and sensitive Alnus firma trees. Resistant and sensitive Alnus firma individuals near Yochon industrial complex were selected to conduct this study in 1997. Photosynthetic capacity, stomatal conductance, transpiration, Rubisco, SOD and GR activities of resistant trees which had no visible damages to air pollution were higher than those of sensitive trees in same area. All physiological results supported that biochemical process to be one of the important key features to understand resistance to air pollution. Increases of photosynthetic capacity and antioxidant enzyme activity in resistant trees in response to air pollution were the results of biological compensation to stress.

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Enhanced Resistance of Transgenic Sweetpotato (Ipomoea batatas Lam.) Plants to Multiple Environmental Stresses Treated with Combination of Water Stress, High Light and High Temperature Stresses

  • Song, Sun-Wha;Kwak, Sang-Soo;Lim, Soon;Kwon, Suk-Yoon;Lee, Haeng-Soon;Park, Yong-Mok
    • Journal of Ecology and Environment
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    • v.29 no.5
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    • pp.479-484
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    • 2006
  • Ecophysiological parameters of non-transgenic sweetpotato (NT) and transgenic sweetpotato (SSA) plants were compared to evaluate their resistance to multiple environmental stresses. Stomatal conductance and transpiration rate in NT plants decreased markedly from Day 6 after water was withheld, whereas those values in SSA plants showed relatively higher level during this period. Osmotic potential in SSA plants was reduced more negatively as leaf water potential decreased from Day 8 after dehydration treatment, while such reduction was not shown in NT plants under water stressed condition. SSA plants showed less membrane damage than in NT plants. As water stress and high light stress, were synchronously applied to NT and SSA plants maximal photochemical efficiency of PS II ($F_v/F_m$) in NT plants markedly decreased, while that in SSA plants was maintained relatively higher level. This trend of changes in $F_v/F_m$ between SSA plants and NT plants was more conspicuous as simultaneously treated with water stress, high light and high temperature stress. These results indicate that SSA plants are more resistive than NT plants to multiple environmental stresses and the enhanced resistive characteristics in SSA plants are based on osmotic adjustment under water stress condition and tolerance of membrane.