Journal of Ecology and Environment

The Ecological Society of Korea (한국생태학회)
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Enhanced Resistance of Transgenic Sweetpotato (Ipomoea batatas Lam.) Plants to Multiple Environmental Stresses Treated with Combination of Water Stress, High Light and High Temperature Stresses

  • Song, Sun-Wha (Department of Life Science, Cheongju University) ;
  • Kwak, Sang-Soo (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lim, Soon (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kwon, Suk-Yoon (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Haeng-Soon (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Yong-Mok (Department of Life Science, Cheongju University)
  • Published : 2006.10.31
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Abstract

Ecophysiological parameters of non-transgenic sweetpotato (NT) and transgenic sweetpotato (SSA) plants were compared to evaluate their resistance to multiple environmental stresses. Stomatal conductance and transpiration rate in NT plants decreased markedly from Day 6 after water was withheld, whereas those values in SSA plants showed relatively higher level during this period. Osmotic potential in SSA plants was reduced more negatively as leaf water potential decreased from Day 8 after dehydration treatment, while such reduction was not shown in NT plants under water stressed condition. SSA plants showed less membrane damage than in NT plants. As water stress and high light stress, were synchronously applied to NT and SSA plants maximal photochemical efficiency of PS II ($F_v/F_m$) in NT plants markedly decreased, while that in SSA plants was maintained relatively higher level. This trend of changes in $F_v/F_m$ between SSA plants and NT plants was more conspicuous as simultaneously treated with water stress, high light and high temperature stress. These results indicate that SSA plants are more resistive than NT plants to multiple environmental stresses and the enhanced resistive characteristics in SSA plants are based on osmotic adjustment under water stress condition and tolerance of membrane.

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References

  1. Allen RD. 1995. Dissection of oxidative stress tolerance using transgenic plants. Plant Physiol 107: 1049-1054 https://doi.org/10.1104/pp.107.4.1049
  2. Asada K. 1999. The water-water cycle in chloroplasts: scavenging of active oxygens and dissipitation of excess photons. Ann Rev Plant Physiol Plant Mol Biol 50: 601-639 https://doi.org/10.1146/annurev.arplant.50.1.601
  3. Avery M, Gordon J. 1983. New frontiers in agroforestry: consequences of pattern in tree and forage systems. In: Foothills for Food and Forests (Hannaway, DB, ed.). Timber Press, Beaverton, Oregon, USA, pp 261-270
  4. Caimi PG, McCole LM, Klein TM, Kerr PS. 1996. Fructan Accumulation and ucrose Metabolism in Transgenic Maize Endosperm Expressing a Bacillus amyloliquefaciens SacB Gene. Plant Physiol 110: 355-363 https://doi.org/10.1104/pp.110.2.355
  5. Clifford SC, Arndt SK, Corlett JE, Joshi S, Sankhla N, Popp M and Jones HG. 1998. The role of solute accumulation, osmotic adjustment and changes in cell wall elasticity in drought tolerance in Ziziphus mauritiana (Lamk.). J Exp Bot 49: 967-977 https://doi.org/10.1093/jexbot/49.323.967
  6. Dionisio-Sese ML, Tobita S. 1998. Antioxidant responses of rice seedlings to salinity stress. Plant Sci 135: 1-9 https://doi.org/10.1016/S0168-9452(98)00025-9
  7. Donahue JL, Okpodu CM, Cramer CL, Grabau EA, Alscher RG. 1997. Responses of Antioxidants to Paraquat in Pea Leaves (Relationships to Resistance). Plant Physiol 113: 249-257 https://doi.org/10.1104/pp.113.1.249
  8. Evans RD, Black RA, Loescher WH, Fellows RJ. 1992. Osmotic relations of the drought tolerant shrub Artemisia tridentata. Plant Cell Environ 15: 49-59 https://doi.org/10.1111/j.1365-3040.1992.tb01457.x
  9. Fan S, Blake TJ, Blumwald E. 1994. The relative contribution of elastic and osmotic adjustments to turgor maintenance in conditions. woody species. Physiol Plant 90, 408-13 https://doi.org/10.1111/j.1399-3054.1994.tb00406.x
  10. Farquhar GD, Shulze ED, Kupper K. 1980. Responses to humidity by stomata of Nicotiana glauca L. and Colylus avellana L. are consistent with the optimization of carbon dioxide uptake with respect to water loss. Aust J Plant Physiol 7: 315-327 https://doi.org/10.1071/PP9800315
  11. Hsiao TC. 1973. Plant responses to water stress. Ann Rev Plant Physiol 24: 519-570 https://doi.org/10.1146/annurev.pp.24.060173.002511
  12. Jones, MM. 1979. Physiological responses of sorghum and sunflower to leaf water deficit (PhD Thesis). Australian National University, Canberra, Australia
  13. Kawaoka A, Matsunaga E, Endo S, Kondo S, Yoshida K, Shinmyo A, Ebinuma H. 2003. Ectopic expression of a horseradish peroxidase enhances growth rate and increases oxidative stress resistance in hybrid sspen. Plant Physiol 132: 1177-1185 https://doi.org/10.1104/pp.102.019794
  14. Kwon SY, Jeong YJ, Lee HS, Kim JS. Cho KY, Allen RD and Kwak SS. 2002. Enhanced tolerances of transgenic tobacco plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplasts against methyl viologen-mediated oxidative stress. Plant Cell Environ 25: 873-882 https://doi.org/10.1046/j.1365-3040.2002.00870.x
  15. Lewis CE, Tanner GW, Terry WS. 1985. Double vs. single-row pine plantations forwood and forage production. Southern J Appl Forestry 9: 55-61
  16. Lim S, Yang KS, Kwon SY, Paek KY, Kwak SS, Lee HS. 2004. Agrobacterium- mediated genetic transformation and plant regeneration of sweetpotato (Ipomoea batatas). Kor J Plant Biotechnol 31: 267- 271 (in Korean with English abstract) https://doi.org/10.5010/JPB.2004.31.4.267
  17. Lu C, Zhang J. 1999. Effects of water stress on photosystem II photochemistry and its thermostability in wheat plants. J Exp Bot 50: 1199-1206 https://doi.org/10.1093/jexbot/50.336.1199
  18. Martinez JP, Silva H, Ledent JF, Pinto M. 2006. Effect of drought stress on the osmotic adjustment, cell wall elasticity and cell volume of six cultivars of common beans (Phaeolus vulgaris L.). Europ J Agro Doi :10.1016/j.eja. 2006. 08. 003
  19. McKersie BD, Bowl ey SR, Jones KS. 1999. Winter survival of transgenic alfalfa overexpressing superoxide dismutase. Plant Physiol 119: 839-848 https://doi.org/10.1104/pp.119.3.839
  20. Nunes MA, Catarino F, Pinto E. 1989. Strategies for acclimation to seasonal drought in Ceratonia siliqua leaves. Physiol Plant 77: 150-156 https://doi.org/10.1111/j.1399-3054.1989.tb05991.x
  21. Oh SJ, Song SI, Kim YS, Jang HJ, Kim SY, Kim M, Kim YK, Nahm BH, Kim JK. 2005. Arabidopsis CBF3/DREB1A and ABF3 in transgenic rice increased tolerance to abiotic stress without stunting growth. Plant Physiol 138: 341-351 https://doi.org/10.1104/pp.104.059147
  22. Olofsson J, Hulme PH, Oksanen L, Suominen O. 2005. Effects of mammalian herbivores on revegetation of disturbed areas in the foresttundra ecotone in northern Fennoscandia. Landscape Ecol 20: 351- 359 https://doi.org/10.1007/s10980-005-3166-2
  23. Osmond B, Ananyev G, Berry J, Langdon C, Kolber Z, Lin G, Monson G, Nichol C, Rascher U, Schurr Um Smith S, Yakir D. 2004. Changing the way we think about global change research: scaling up in experimental ecosystem science. Global Change Biology 10: 393-407 https://doi.org/10.1111/j.1529-8817.2003.00747.x
  24. Premachandra GS, Saneoka H, Fujita K, Ogata S. 1992. Leaf water relations, osmotic adjustment, cell Membrane stability, epicuticular wax load and growth as affected by increasing water deficits in sorghum. J Exp Bot 43: 1569-1576 https://doi.org/10.1093/jxb/43.12.1569
  25. Radin JW. 1983. Physiological consequences of cellular water deficits: osmotic adjustment. In: Limitations to efficient water use in crop production (Taylor JM, Rains DW, Sinclair TR. eds). American Society for Agronomy (Publ.). pp. 267-76
  26. Samis K, Bowley S, McKersie B. 2002. Pyramiding Mn-superoxide dismutase transgenes to improve persistence and biomass production in alfalfa. J Exp Bot 53: 1343-1350 https://doi.org/10.1093/jexbot/53.372.1343
  27. Schreiber U, Bilger W, Neubauer C. 1994. Chlorophyll fluorescence as a noninstrutive indicator for rapid assessment of in vivo photosynthesis. In: Ecophysiology of Photosynthesis (Shulze ED, Caldwell MM. eds). Springer, Berlin, pp 49-70
  28. Sen Gupta A, Heinon J, Holaday A, Burke J, Allen R. 1993. Increased resistance to oxidative stress in transgenic plants that overexpress chloroplastic Cu/Zn superoxide dismutase. Proc Natl Acad Sci USA 90: 1629-1633
  29. Suzuki S, Murai N, Burnell JN, Masao A. 2000. Changes in Photosynthetic Carbon Flow in Transgenic Rice Plants That Express $C_4$- Type Phosphoenolpyruvate Carboxykinase from Urochloa panicoides. Plant Physiol 124: 163-172 https://doi.org/10.1104/pp.124.1.163
  30. Van Camp W, Capiau K, Van Montagu M, Inze D, Slooten L. 1996. Enhancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts. Plant Physiol 112: 1703-1714 https://doi.org/10.1104/pp.112.4.1703
  31. Torsethaugen G, Pitcher LH, Zilinskas BA, Pell EJ. 1997. Overproduction of Ascorbate Peroxidase in the Tobacco Chloroplast Does Not Provide Protection against Ozone. Plant Physiol 114: 529-537 https://doi.org/10.1104/pp.114.2.529