• Journal of Korean Society of Forest Science
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• v.96 no.3
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• pp.369-375
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• 2007

We examined two Populus species (Populus euramericana and Populus alba ${\times}$ Populus glandulosa) trees grown under livestock waste leachate treatment in the field. We investigated their physiological properties (photosynthetic rate, chlorophyll contents, transpiration rate and intercellular $CO_2$ concentration) and antioxidant enzyme activities. Ascorbate peroxidase and glutathione reductase were analyzed. According to our measurements, P. euramericana at control site showed a lower total chlorophyll contents, photosynthetic rate, intercellular $CO_2$ concentration, transpiration and stomatal conductance than those of trees at treatment site. P. alba ${\times}$ P. glandulosa showed low stomatal conductance and low photosynthetic rate.

• Korean Journal of Medicinal Crop Science
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• v.22 no.1
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• pp.38-45
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• 2014

This study was performed to investigate the physiological responses of Oplopanax elatus by water condition. Drought stress was induced by withholding water for 26 days. The results show that $P_{N\;max}$, SPAD, gs, E and Ci were significantly decreased with decreasing of soil moisture contents. However, AQY and WUE were decreased slightly only at 26 day. This implies that photosynthetic rate is reduced due to an inability to regulate water and $CO_2$ exchange through the stomatal. According to JIP analysis, ${\Phi}_{PO}$, ${\Psi}_O$, ${\Phi}_{EO}$ and $PI_{ABS}$ were dramatically decreased at 21 day and 26 day, which reflects the relative reduction state of the photosystem II. On the other hand, the relative activities per reaction center such as ABS/RC, TRo/RC were significantly increased at 26 day. Particularly, Dio/RC and DIo/CS increased substantially under drought stress, indicating that excessive energy was consumed by heat dissipation. These results of chlorophyll a fluorescence show that the sensitivity changes photosystem II activity. Thus, according to the results, O. elatus was exhibited a strong reduction of photosynthetic activity to approximately 10% soil moisture contents, and JIP parameters could be useful indicator to monitor the physiological states of O. elatus under drought stress.

• Korean Journal of Medicinal Crop Science
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• v.26 no.5
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• pp.362-369
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• 2018

Background: For stable induction of tetraploidy in Fallopia multiflora Haraldson, colchicine was treated to establish the condition of induction and investigated the morphological and cytogenetic traits of the tetraploid plants obtained compared to those of diploid ones. Methods and Results: For the induction of tetraploidy, F. multiflora plants were soaked in aqueous solutions of colchicine at various concentration (0.1, 0.5, and 1.0%). After this, 2% dimethyl sulfoxide (DMSO) was added at room temperature on a shaker set at 150 rpm for periods of 12, 24, and 48 h. The induction rate of tetraploids appeared to be the highest in plants treated with 0.5% colchicine for 24 h. As the colchicine concentration and soaking time increased above these levels, the growing tip of the roots did not develop and they began to rot. When compared to diploid plants, tetraploids differed greatly in various characteristics, including the sizes and shapes of the leaves, fruits, flowers and roots. The induced tetraploid F. multiflora had larger guard cells, and chloroplasts, increased number of chloroplast in the guard cells and decreased stomatal densities. Conclusions: When colchicine induced plants for tetraploid, it can be distinguished from diploids, in various characteristics such as morphological changes as stomatal size, number of chloroplasts per guard cell, number of chromosomes and flow cytometry. Therefore, it proved that these methods are suitable, quick and easy methods for the identification of the ploidy level of F. multiflora.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.2
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• pp.201-204
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• 1988

To find out the relationship between the eco-physiological characteristics and wind damage of rice leaves by typhoon 'Thelma' occurred at young panicle formation stage of rice plant, the study was carried out. The rate of dead leaves by wind damage in Indica X Japonica varieties were higher than that in Jap. varieties. Ind. X Jap. varieties which had erect and broader leaves had the higher number of silicified cell, moisture loss and stomatal aperture in leaves. And the wind damage were positvely correlated with the characteristics such as the number of silicified cell (r=0.7546$\^$*/), rate of moisture loss (r=0.8343$\^$**/) and stomatal aperture (r=0.8460$\^$**/) of rice leaf blade etc.

• Korean Journal of Environmental Agriculture
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• v.28 no.2
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• pp.106-112
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• 2009

The increased $CO_2$ and temperature (700 ${\mu}$mol.$mol^{-1}$ $CO_2$ and $30^{\circ}C$) was compared with ambient growth conditions (400 ${\mu}$mol.$mol^{-1}$ $CO_2$ and $25^{\circ}C$) in hot pepper. Gas exchange measurements, including net photosynthesis ($P_{net}$) and stomatal conductance ($g_s$), were taken according to treatment in fields of peppers grown in Suwon and Asan during 2008. The increased treatment $P_{net}$ by 35-45% throughout the season and was statistically significant in t-tests (p < 0.001); however, it did not significantly affect $g_s$. In addition, the gas exchange parameters in sun and shade leaves were measured. The difference between the sun and shade leaves was much greater than that between the elevated and ambient treatments, especially at harvest. Four commercial cultivars of hot pepper, Chunhasangsa, Ryukang, Manitta, and Olympic, were also compared by ANOVA. Chunhasangsa had the highest $P_{net}$, which decreased by 30% from the vegetative to the harvest stage. Based on a factorial design, the effect of the increased $CO_2$ and temperature was assessed based on the temperature, $CO_2$, and their interaction effects. Orthogonal contrasts showed that the effects of temperature on $P_{net}$ and $g_s$ were significant, whereas $CO_2$ and their interactions were not.

• Journal of Plant Biology
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• v.38 no.1
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• pp.79-86
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• 1995

Visible injury symptoms such as necrosis, chlorosis and premature senscence in the leaves of Ginkgo bloba and Pinus thunbergii treated with acid rain of pH 3.2 or below were observed. The epicuticular wax erosions were observed by SEM after exposure to acid rain of pH 2.4 and 3.2 in G. biloba and pH 4.0 below in P. thunbergii. The adaxial epidennal cells and sponge parenchyma cells were compressed, and those were distorted in the leaves of G. biloba treated with simulated acid rain of pH 3.2 or below. However, vascular tissue was intact. With increase of acidity, mesophyll cells were smaller than those of control while intercellular space in mesophyU was increased. In P. thunbergii, sponge parenchyma cells and vascular tissue except epidennis were distorted after exposure to acid rain of pH 2.4. The size change of stomata in foliar injury was not observed, but the stomatal index and size of stomatal aperture in leaves treated with acid rain increased. The stomata of injured leaf were opened in both species examined.amined.

• The Korean Journal of Ecology
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• v.26 no.4
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• pp.165-172
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• 2003

The objectives of this study were to examine the physiological responses to ozone and to measure ozone uptake rates of Betula species exposed to relatively high concentration of pollutants. At the end of the growing season, photosynthesis, pigments contents, antioxidants (SOD and GR) and ozone uptake rates were measured or estimated at the leaves of five Betula species (Betula costata, B. davurica, B. platyphylla var. japonica, B. schmidtii and B. ermanii) exposed to 100ppb ozone concentration. On the termination of the experiment, growth effects were determined by measuring leaf area and dry weights of leaf, stem and root. Ozone treatment showed the significant reduction the leaf area and dry weight of four Betula species, except for B. ermanii. Shoot / root (SR) ratio of five species represented two different types. SR ratio of B. costata and B. davurica were lower than control, in contrast, SR ratio of B. platyphylla var. japonica, B. schimidtii and B. emani, were higher than that of control. The photosynthetic responses of five species were different in responses to ozone exposure. Four species, except for B. emanii, maintained or increased the stomatal conductance, but B. emanii decreased both stomatal conductance and photosynthesis. SOD activities of five species decreased by the ozone exposure, especially B. ermanii showed the largest reduction, GR activities of B. platyphylla var. japonica and B. schmidtii increased, B. costata and B. emanii decreased. Instantaneous ozone uptake rate was the highest at the leaves of B. ermanii and B. costata, ozone uptake per seedling was the highest at the leaf of B. schmidtii and B. emanii. It was concluded that B. costata, B. davurica and B. platyphylla var. japonica, appeared the growth reduction and visible ozone injury, were sensitive species to ozone, and B. schmidtii with the increased antioxidant activity and B. ermanii without the growth reduction were relatively resistant species to high ozone concentration at the early growing stage.

• The Korean Journal of Ecology
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• v.3 no.1_2
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• pp.31-39
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• 1980

Light energy utilization was investigated in the fallen stems of Opuntia bigelovii. Threshold time for the decreasing steady state of acid accumulation in the palisade tissue of Opuntia stems was 4 hours under 1,000 $\mu Em^{-2}sec^{-1}$ of PAR at $75^{\circ}C$, while stomatal closing throughout the stem stage was illustrated by 256.0-310.4 sec $\textrm{cm}^{-1}$ of stem diffusive resistance and 0.20g $day^{-1}$ of the water loss rate as cuticular resistance. The acid loss rate in the stems per 4 hours was related to tissue water contents and a few acid loss rate could be recognized at the water content rage of 56.4%~46.8%. Endogenous oscillation of tissue acidity due to the diurnal rhythmic phenomena depended on the tissue water content was found in the Opuntia stems with stomatal closing during the normal day/night cycle. The survival rate of 1 segment to survive 2 years old cactus was 22.7% in desert environments. Such a compensation photosynthesis which utilizes light energy and maintains the reassimilation of endogenous gases was interpreted as conceptual model.

• Korean Journal of Soil Science and Fertilizer
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• v.51 no.2
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• pp.79-89
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• 2018

Subsurface drip irrigation (SDI) system is considered one of the most effective methods for water application. A 2-year field study was conducted to investigate the effect of SDI systems with various dripline spacing (0.7 or 1.4 m) and position (under furrow or ridge) on soybean (Glycine max L.) production at a sandy-loam soil in Miryang, South Korea. For 2016-2017, average grain yield in SDI irrigated plots, $3.16Mg\;ha^{-1}$, was statistically greater than rainfed irrigated plot ($2.63Mg\;ha^{-1}$). Soybean grain yield averaged $3.25Mg\;ha^{-1}$ for the 0.7 m dripline spacing and $3.07Mg\;ha^{-1}$ for the 1.4 m spacing for the two-year period compared to a rainfed irrigated average of $2.63Mg\;ha^{-1}$ for the same period. Soybean treated with SDI system had significantly greater values of normalized difference vegetation index and stomatal conductance, indicating that soybean plants in SDI plots had greater photosynthetic and stomatal activity due to the higher water availability in soil. Irrigation water use efficiency (IWUE) was greatest in the plot of 0.7 m spacing installed under ridge position than any other plot across growing season. Average soil water content in plots with 0.7 m dripline spacing was $0.21m^3\;m^{-3}$ at 5 cm depth layer, which was 45% greater compared to the plots with 1.4 m spacing, even though the gross irrigation amounts were greater in 1.4 m spacing plots. It is concluded that wide dripline spacing (1.4 m) is probably the more economical installation design for SDI system compared to 0.7 m spacing in this study soil because the initial cost for dripline may be reduced with wide spacing design, even though the IWUE is greater in the plot of 0.7 m dripline spacing.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.2
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• pp.146-152
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• 1997

Development of shoot and root, leaf water potential and photosynthetic rate affected by water stress in early growing stage of tobacco were surveyed to interpret stress response in terms of plant physiological and agricultural aspects. The growth of shoot and root was highly suppressed by water stress and the difference in dry weight by rewatering was smaller in root than in shoot. The total root length was highly decreased by water stress and the lengths of root for water stress and non-stress were 74m and 84m, respectively, after rewatering. The root growth treated by water stress was increased between 2nd and 3rd day after treatment indicating that temporary water stress at early growing stage might have increased of root zone activity for early growth stage. The leaf water potentials were decreased to -7.63MPa, -9.47MPa, -11.89MPa, -13MPa at the 2nd, 3rd, 4th and 5th day by water stress. The relative water contents were 75%, 62% and 57% at the 3rd, 4th and 5th day after treatment. Photosynthesis was reduced largely by water stress. The photosynthetic rate after treatment at 2nd day and 3rd day was dropped to 18.15$\mu$mol. $CO_2$/$m^2$ㆍsec$^{-1}$ and 9.35$\mu$mol. $CO_2$/$m^2$ㆍsec$^{-1}$. It was never recovered to the normal, even after rewatering. Stomatal conductance had been reduced since 2nd day after treatment and increased after rewatering.