• 한국어류학회지
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• 제31권1호
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• pp.23-29
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• 2019

이번 연구에서 바리과 어종의 새로운 교잡종을 생산하기 위한 연구의 일환으로 능성어 (Hyporthodus septemfasciatus) ♀${\times}$대왕바리 (Epinephelus lanceolatus) ♂ (CGGG)의 발생 양상, 부화율, 기형 발생률 그리고 부화 소요시간을 조사하고, 교잡종의 모계어종의 순종 (능성어 ♀${\times}$♂, CG)과 비교하였다. CGGG와 CG 수정란은 자외선 살균 해수가 채워진 5-L 용량의 비이커 9개에 3,000~5,000개/비이커의 밀도로 수용되었으며, 발생 기간 중 수온은 $23.5{\sim}24.8^{\circ}C$ 그리고 염분은 32.1~32.8 ppt 였다. CGGG의 수정률과 부화율은 각각 $69.4{\pm}1.5%$와 $59.0{\pm}5.1%$로서 CG에 비해 유의하게 낮았다 (p<0.05). CGGG는 비균등 난할, 배체 체형 기형 그리고 부화자어의 척추 기형이 높게 출현한 것을 제외하고, CG와 동일한 난발생 양상을 보였다. CGGG의 부화까지 소요 시간은 CG에 비해 약 2시간 느렸다(p<0.05). 이상의 결과에서 CGGG는 수정률, 부화율 그리고 기형률 등 발생능력이 CG에 비해 낮았지만 부화자어의 대량생산이 가능할 것으로 판단된다.

• 한국전자통신학회논문지
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• 제16권5호
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• pp.981-990
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• 2021

노후화된 버스 정류장에서 코로나19 방역이 이행되지 않는 경우가 많다. 이러한 문제점들을 개선하기 위해 본 연구를 진행했다. 쉘터 내부와 외부를 UV 광촉매 코팅하여 멸균을 시켜 위생적이고 청결한 쉘터를 유지할 수 있으며, CO₂ 센서와 팬을 이용하여 쉘터 내의 이산화탄소를 감소시켜 쾌적한 공기를 유지함으로써 승객들은 원활하게 호흡 할 수 있게 된다. 또한 체온 센서를 사용하여 체온을 측정해 코로나19 감염 의심 환자를 1차로 격리시켜 쉘터 내 코로나19 바이러스 확산을 20%이상 방지할 수 있다고 예상된다. 체온 측정 거리가 10cm 이내 일 때 체온 측정의 정확성은 98.9%로 확인할 수 있었다. 쉘터 내부의 무선 통신을 이용한 승차 벨을 사용하여 버스 기사님과 접촉하지 않으면서 안전하고 명확한 승차의사를 전달 할 수 있다.

• 한국물환경학회지
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• 제27권3호
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• pp.334-341
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• 2011

The free residual chlorine of tap water samples, collected from 266 faucets on the water pipe network in Daegu City, was between 0.1 and 0.79 mg/L. On microorganic tests, general bacteria and the coliform goup were not detected and thus the tap water was turned out to be fit to drink. In particular, samples of which free residual chlorine was 0.1 mg/L and over were cultured in R2A agar media at $25^{\circ}C$ for 7 days, and as a result heterotrophic bacteria were detected in 65.9% of samples; (1). The closer tap water got to the faucet from the stilling basin, the lower residual chlorine concentration became but the more the bacterial count became. And, more bacteria were detected in the R2A agar medium than in the PCA medium. (2). In the case of separated strains, most colonies were reddish or yellowish. 16S rRNA sequence was identified as Methylobacterium sp. and Williamsia sp., and yellow strain was identified as Sphingomonas sp., Sphingobium sp., Novosphingobium sp., Blastomonas sp., Rhodococcus sp. and Microbacterium sp. White strain was identified as Staphylococcus sp. (3). Sterilized tap water in polyethylene bottles was inoculated with separated strain and was left as it was for 2 months. As a result, bio-film was observed in tap water inoculated with Methylobacterium sp. and Sphingomonas sp. It was found that heterotrophic bacteria increased when free residual chlorine was removed from tap water in the water pipe network. Thus, there is a need to determine a base value for heterotrophic bacteria in order to check the cleanliness of tap water in the water pipe network.

• 상하수도학회지
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• 제35권3호
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• pp.227-235
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• 2021

This study examined the effect of ultraviolet (UV) application on bacterial disinfection in a commercialized humidifier using ultrasonic wave (UW). To accurately examine disinfection kinetics in tap-water condition, tap-water was sterilized using a filter, and then inoculated with pure cultures of E. coli and P. putida with known viable counts. The disinfection kinetic characteristics were experimentally compared when UV alone, UW alone, and UW+UV together were applied in disinfecting the added bacteria in the commercialized humidifier. When UV alone was applied, bacterial disinfection kinetics followed a first-order decay reaction, and showed an approximately 10-time weaker disinfection compared to the typical UV disinfection in water treatment or wastewater treatment. When UW alone was applied, bacterial disinfection kinetics followed a second-order decay reaction with a low disinfection rate constant of 0.0002 min^-1(CFU/mL)^-1. When UV and UW were applied together, however and interestingly, the disinfection rate constant (0.0211 min^-1(CFU/mL)^-1) was approximately 100 times increased than that for the UW alone case. These results revealed that the co-use of UV and UW can provide synergistic effect on bacterial disinfection in a tap-water condition in household humidifiers.

• 산업융합연구
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• 제19권6호
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• pp.67-72
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• 2021

건물에 설치된 엘리베이터는 승강기 호출 버튼과 목적층으로의 선택을 위한 입력 버튼으로 구성되어 있다. 엘리베이터 버튼은 승강기를 이용하는 사람이 직접 눌러야 입력이 된다. 이러한 승객의 입력은 버튼의 오염으로 인해 전염병에 감염될 수 있다. 이러한 문제점을 해결하기 위한 수단으로 비접촉식 버튼이 요구되고, 이는 정전용량 방식을 적용하여 물체의 근접을 감지한다. 적외선 열감지 센서를 부착하여 인체의 체온을 측정하는 기능을 구현하고 UV-LED를 부착하여 버튼의 살균 기능을 제공한다. 물체가 없을 때의 출력파형을 측정하고 물체가 근접할 때의 출력 파형을 측정하여 버튼이 선택되고 적외선온도측정 센서를 통해 체온이 측정되며, 사용자가 없을 때 UV-LED를 점등하여 살균하였다. 비접촉식 엘리베이터 버튼은 전염병을 옮기는 바이러스의 감염을 방지할 수 있는 효과가 있고, 체온을 감지하여 CIVID 19의 양성 확진자를 선별할 수 있어 감염병 예방에도 효과가 있을 것으로 기대된다.

• Journal of Plant Biotechnology
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• 제49권3호
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• pp.213-221
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• 2022

Wild garlic (Allium victorialis var. platyphyllum, AVVP) is a nontimber forest product used as an edible and medicinal vegetable. AVVP is usually propagated form offspring bulbs but it takes a long time to harvest. Using tissue culture technology could overcome this problem. This study investigated the optimal conditions for shoot multiplication, root growth, and plant growth by scooping AVVP bulbs. AVVP bulbs harvested from Ulleung Island, Korea, the main producer of AVVP, were surface-sterilized and used for in vitro propagation. Shoot multiplication was performed by the scooping method. More than five multiple shoots were induced from scooped tissue in Quoirin and Lepoivre (QL) medium containing plant growth regulators (PGRs); the maximum number of multiple shoots were induced from scooped tissue in QL medium containing 0.45 μM thidiazuron (TDZ) after 16 weeks of culture. Roots were induced directly at the base of the shoots in all treatments. In vitro rooting depended on the type of PGRs, and the best root-inducing treatment was QL medium containing 9.84 μM indole-3-butyric acid (IBA). Plants with in vitro roots were transferred to pots containing artificial soil and successfully acclimatized for 4 weeks. The acclimatized plants showed a survival rate of 80% after 20 weeks and gradually promoted growth depending on the acclimatization period. The results of this study will be of great help to AVVP dissemination through sustainable mass propagation.

• Journal of Forest and Environmental Science
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• 제38권3호
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• pp.141-151
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• 2022

Soil seed banks as natural storage of plant seeds play an important role in the maintenance and regeneration of watershed. Natural regeneration potential of the soil seed bank of Land use types (LUTs) in Ogun River watershed (ORW) was investigated. ORW was stratified using proportionate sampling technique into Guinea Savannah (GS), Rainforest (RF) and Swamp Forest (SF) Ecological Zones (EZs). Three LUTs: Natural Forest (NF), Disturbed Forest (DF) and Farmland (FL) were purposively selected in GS: GSNF, GSDF, GSFL; RF: RFNF, RFDF, RFFL and SF: SFNF, SFDF, SFFL, respectively. Systematic line transects was used in the laying of the sample plots. Sample plots of 25 m×25 m were established in alternate positions. Ten 1 m×1 m quadrats were randomly laid for soil core sampling from previously randomly selected ten plots. The core samples (10) were pooled per plot in each LUT and placed in individual trays. Ten trays with sterilized soil were used as control. The trays were watered regularly and checked for seedlings emergence fortnightly for 18 months. The experimental design used was 3×3 factorial experiments. ANOVA, Diversity index (H') and Similarity index (SI) were used to analyze the data. There was significant difference in seedling emergence among ecological zones and land use types (p<0.05). A total of 4,400 seedlings emerged from the soil samples. All species were distributed among 32 families. FL in the RF had the highest number of germinated seeds (705±37.33 seedlings) followed by DF in the RF (701±49.6 seedlings). The lowest emergence was in NF of the SF (199±28.41 seedlings). DF in the RF had highest number of species (34) distributed among 22 families. Emergence from soil seed bank of NF in ORW was generally with more of tree species than herbs that were predominant in FL and DF.

• 치위생과학회지
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• 제22권3호
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• pp.191-198
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• 2022

Background: The purpose of this study was to evaluate the composition of the crystal phases of various calcium silicate-based materials (CSMs): ProRoot white MTA^Ⓡ (mineral trioxide aggregate) (WMTA), Ortho MTA^Ⓡ (OM), Endocem MTA^Ⓡ (EM), Retro MTA^Ⓡ (RM), Endocem Zr^Ⓡ (EN-Z), Biodentine^TM (BD), EZ-seal^TM (EZ), and OrthoMTA III (OM3). Methods: In a sample holder, 5 g of the powder sample was placed and the top surface of the material was packed flat using a sterilized glass slide. The prepared slides were mounted on an X-ray diffraction (XRD) instrument (D8 Advance; Bruker AXS GmbH, Germany). The X-ray beam 2θ angle range was set at 10~90° and scanned at 1.2° per minute. The Cu X-ray source set to operate at 40 kV and 40 mA in the continuous mode. The peaks in the diffraction pattern of each sample were analyzed using the software Diffrac (version 2.1). Then, the peaks were compared and matched with those of standard materials in the corresponding Powder Diffraction File (PDF-2, JCPDS International Center for Diffraction Data). A powder samples of the materials were analyzed using XRD and the peaks in diffraction pattern were compared to the Powder Diffraction File data. Results: Eight CSMs showed a similar diffraction pattern because their main component was calcium silicate. Eight CSMs showed similar diffraction peaks because calcium silicate was their main component. Two components were observed to have been added as radiopacifiers: bismuth oxide was detected in WMTA, OM, and EM while zirconium oxide was detected in RM, EN-Z, BD, EZ, and OM3. Unusual patterns were detected for the new material, OM3, which had strong peaks at low angles. Conclusion: It was caused by the presence of Brushite, which is believed to have resulted in crystal growth in a particular direction for a specific purpose.

• 대한의생명과학회지
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• 제28권3호
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• pp.186-191
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• 2022

The use of earphones has recently been widely used around the world. In currently, students wear earphones a lot in a daily life. The types of earphones are open-earphones, Canalphones, and headphones. Many students don't periodically to sterilization their earphones. Therefore, it can be an incubator that can induced ear infections. The objective of this study was to detect the pathogenic bacteria from the earphones used by the students. A total of 3 type earphones swabs were collected by sterile cotton swabs. The swabs were inoculated onto BHI agar and incubated aerobically 48 hour at 37℃. 16s rRNA PCR, electrophoresis and sequencing were performed to confirm the identification of all the bacterial isolates. As a result, 24 pathogens were identified in sequencing. Three types of earphones were sterilized in three ways: ultraviolet (UV), 70% ethyl alcohol, and antibacterial wet tissue. If you use earphones for a long time without disinfecting them for a long time, it causes various diseases such as external ear infections. The findings of this study the users periodically to sterilization their respective earphones.

• 한국작물학회지
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• 제31권1호
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• pp.56-61
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• 1986

옥수수품종 (Dekalb XL 72B)과 대두품종 ( Willi -ams)의 발아와 묘신장을 두 온도, 세 토양수분 포텐셜 및 네 polyethylene glycol(PEG) 8,000 수준에서 측정하였다. 각 품종의 20처리종자를 0.2% Thiram에 처리하여 가비중 1.20으로 압축된 살균사양토에 2.0cm 깊이로 파종 분할구배치법 사반복으로 실시하였다. 1. PEG로 한 삼투처리는 고온에서나 저토양수분포텐셜 조건에서 효과가 거의 없었다. 2. 대두는 옥수수보다 높은 묘수분함량을 보였고 두 작물 모두 토양수분 포텐셜과 온도가 증가할수록 수분흡수가 증가되었다. 3. 옥수수의 묘장이 35$^{\circ}C$에서는 대두보다 길었으나 15$^{\circ}C$에서는 짧았다. 4. 옥수수의 건물중은 35$^{\circ}C$에서 감소되었고 대두의 건물중은 토양수분 포텐셜이 증가할수록 감소되었다.