Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
권호 표지

Embryonic Development of Fertilized Eggs of Convict Grouper (Hyporthodus septemfasciatus) ♀×Giant Grouper (Epinephelus lanceolatus) ♂

능성어 (Hyporthodus septemfasciatus) ♀×대왕바리 (Epinephelus lanceolatus) ♂ 수정란의 난발생

  • Received : 2019.03.15
  • Accepted : 2019.03.25
  • Published : 2019.03.31
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Abstract

The embryonic development and hatchability of the artificially fertilized eggs of convict grouper (Hyporthodus septemfasciatus) ♀${\times}$giant grouper (Epinephelus lanceolatus) ♂ (CGGG) were compared to those of the maternal pure species (convict grouper ♀${\times}$♂, CG) to establish a novel grouper hybrid for aquaculture industry. The fertilized eggs were divided into nine 5-L beakers (3,000~5,000 eggs/beaker) filled with UV sterilized seawater and incubated at a temperature range of $23.5{\sim}24.8^{\circ}C$ (32.1~32.8 psu). The percentages of fertilization and hatching of CGGG were $69.4{\pm}1.5%$ and $59.0{\pm}5.1%$, respectively and were significantly lower than those of CG (p<0.05). The CGGG proceeded normal embryonic development similar to that of CG, but showed an irregular cleavage, immature embryonic body and spinal deformity in hatched larvae. The incubation time from fertilization to hatching of CGGG was 31 hrs, which was approximately 2 hrs slower than that of CG. Our study provided the possibility of mass production of grouper hybrid CGGG larvae.

이번 연구에서 바리과 어종의 새로운 교잡종을 생산하기 위한 연구의 일환으로 능성어 (Hyporthodus septemfasciatus) ♀${\times}$대왕바리 (Epinephelus lanceolatus) ♂ (CGGG)의 발생 양상, 부화율, 기형 발생률 그리고 부화 소요시간을 조사하고, 교잡종의 모계어종의 순종 (능성어 ♀${\times}$♂, CG)과 비교하였다. CGGG와 CG 수정란은 자외선 살균 해수가 채워진 5-L 용량의 비이커 9개에 3,000~5,000개/비이커의 밀도로 수용되었으며, 발생 기간 중 수온은 $23.5{\sim}24.8^{\circ}C$ 그리고 염분은 32.1~32.8 ppt 였다. CGGG의 수정률과 부화율은 각각 $69.4{\pm}1.5%$와 $59.0{\pm}5.1%$로서 CG에 비해 유의하게 낮았다 (p<0.05). CGGG는 비균등 난할, 배체 체형 기형 그리고 부화자어의 척추 기형이 높게 출현한 것을 제외하고, CG와 동일한 난발생 양상을 보였다. CGGG의 부화까지 소요 시간은 CG에 비해 약 2시간 느렸다(p<0.05). 이상의 결과에서 CGGG는 수정률, 부화율 그리고 기형률 등 발생능력이 CG에 비해 낮았지만 부화자어의 대량생산이 가능할 것으로 판단된다.

Keywords

References

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