Egg Development and Early Life History of the Korean Endemic Sand Spine Loach, Cobitis nalbanti (Pisces: Cobitidae)

한국고유종 점줄종개 Cobitis nalbanti의 난발생 및 초기생활사

  • Received : 2019.02.25
  • Accepted : 2019.03.25
  • Published : 2019.03.31

Abstract

Egg development and early life history of the Korean endemic sand spine loach, Cobitis nalbanti, were investigated in the present study. Adult fish were sampled using spoon nets at the Yeongsan River in Seongam-ri, Bukha-myeon, Jangseong-gun, Jeollanam-do, Korea, June 2011. Eggs were obtained after injecting Ovarprim into females. Eggs were then artificially fertilized using the dry method in the laboratory. Mature eggs were transparent and slightly adhesive with light yellowish coloring, measured $0.99{\pm}0.03mm$ ($mean{\pm}SD$) in diameter. And number of spawned eggs were $1,527{\pm}410$ per individual. Hatching (50%) of the embryo occurred 52 hours after fertilization a water temperature of $25^{\circ}C$, and the average newly hatched larvae size was about $4.2{\pm}0.22mm$ in total length. At fifth day after hatching, the larval total length reached $6.0{\pm}0.34mm$ on average and their yolk sac had been completely absorbed. At fifteen day after hatching, larva entered the juvenile stage and reached $10.8{\pm}0.45mm$ in total length. At 100th day after hatching, the formation of Gambetta's zone of four dotted line was complete and juveniles were similar in general appearance to adults, and they averaged $41.1{\pm}2.95mm$ in total length.

한국고유종 점줄종개 Cobitis nalbanti의 난발생 및 초기생활사를 조사하였다. 성어는 2011년 6월 영산강 상류인 전남 장성군 북하면 성암리에서 족대를 이용하여 채집하였다. 채집된 성숙한 친어는 복강에 Ovaprim을 주사하고, 12시간 경과 후 복부압박법으로 채란하고 건식법으로 인공수정시켰다. 산란된 성숙란은 약한 점착성을 띤 밝은 황색의 분리침성난으로 난경은 $0.99{\pm}0.03mm$이고 산란수는 $1,527{\pm}410$개였다. 수온 $25^{\circ}C$에서 수정 후 52시간 후에 50%가 부화하였으며, 크기는 전장 $4.2{\pm}0.22mm$였다. 부화 5일 후에 전장 $6.0{\pm}0.34mm$로 난황이 모두 흡수되어 후기자어로 이행하였으며, 15일 후에는 전장 $10.8{\pm}0.45mm$로 지느러미 기조가 모두 정수로 되어 치어기로 이행하였다. 부화 후 100일 후에는 전장 $41.1{\pm}2.95mm$로 4개의 점줄로 이루어진 Gambetta's zone이 뚜렷하고 외형은 성어와 비교적 유사하였다.

Keywords

References

  1. Balon, E.K. 1985. Early life histories of fishes: New developmental, ecological and evolutionary perspectives. dr. W. Junk Publisher, Dordrecht, 280pp.
  2. Bogutskaya, N.G., A.M. Naseka, S.V. Shedko, E.D. Vasil'eva and I.A. Chereshnev. 2008. The fishes of the Amur River: updated check-list and zoogeography. Ichthyol. Explor. Freshwaters, 19: 301-366.
  3. Charles, B.K., W.B. William, R.K. Seth, U. Bonnie and F.S. Thomas. 1995. Stages of embryonic development of the Zebrafish. Dev. Dyn., 203: 253-310. https://doi.org/10.1002/aja.1002030302
  4. Chyung, M.K. 1977. The Fishes of Korea. Illji Publishing, Seoul, 727pp.(in Korean)
  5. Kawanabe, H., N. Mizuno and K. Hosoya. 1989. Freshwater Fishes of Japan. YAMA-KEI Publishers Co., Ltd., Tokyo, 720pp.
  6. Kim, I.S. 1980. Systematic studies on the fishes of the fishes of the family Cobitidae (Order Cypriniformes) in Korea. 1. Three unrecorded species and subspecies of the genus Cobitis from Korea. Korean J. Zool. 23: 239-250.
  7. Kim, I.S. 1997. Illustrated Encyclopedia of Fauna & Flora of Korea, Vol. 37, Freshwater Fishes. Ministry of Education, Yeongi, 629pp.(in Korean)
  8. Kim, I.S. 2009. A review of the spined loaches, family Cobitidae (Cypriniformes) in Korea. Korean J. Ichthyol., 21 (supplement): 7-28.
  9. Kim, I.S. and E.H. Lee. 1995. Studies on early embryonic development of Niwaella multifasciata (Pisces: Cobitidae). Korean J. Limnol., 28: 455-462.(in Korean)
  10. Kim, I.S. and G.Y. Lee. 1988. Taxanomic study of the cobitid fish, Cobitis nalbanti Rendal and C. striata Ikeda (Cobitidae) from Korea. Korean J. Syst. Zool., 4: 91-102.(in Korean)
  11. Kim, I.S. and J.H. Lee. 1986. A chromosomal study on the genus Cobitis (Pisces: Cobitidae) in the southern part of Korea. Bull. Korean Fish. Soc., 19: 257-564.(in Korean)
  12. Kim, I.S. and J.Y. Park. 1992. Sex rations and hermaphroditism of Cobitis lutheri(Pisces, Cobitidae) from Korea. Korean J. Ichthyol., 4: 72-76.
  13. Kim, I.S. and J.Y. Park. 2007. Freshwater Fishes of Korea. Kyohak Publishing, Seoul, 465pp.(in Korean)
  14. Kim, I.S. and M.T. Jeong. 1988. Seasonal variation of the color pattern in the Cobitid fish Cobitis taenia lutheri from Korea. Korean J. Ecol., 77-82.(in Korean)
  15. Kim, I.S., M.H. Ko and J.Y. Park. 2006. Population ecology of Korean sand loach Cobitis tetralineata (Pisces; Cobitidae) in the Seomjin River, Korea. J. Ecol. Field Biol., 29: 277-286. (in Korean) https://doi.org/10.5141/JEFB.2006.29.3.277
  16. Kim, I.S. and W.O. Lee. 1984. Effects of stream modification in the Seomjin River on the fish communities of the Dongjin River in Korea. Bull. Korean Fish. Soc., 17: 549-556.(in Korean)
  17. Kim, I.S., Y. Choi, C.L. Lee, Y.J. Lee, B.J. Kim and J.H. Kim. 2005. Illustrated Book of Korean Fishes. Kyohak Publishing Co. Ltd., Seoul, 615pp.(in Korean)
  18. Kim, J.H. and S.Y. Yang. 1993. Systematic studies of the genus Cobitis (Pisces: Cobitidae) in Korea IV. Introgressive hybridization between two spined loach subspecies of the genus Cobitis. Korean J. Zool., 36: 535-544.
  19. Kitagawa, T., S.R. Jeon, E. Kitagawa, M. Yoshioka, M. Kashiwagi and T. Okazaki, 2005. Genetic relationships among the Japanese and Korean striated spined loach complex (Cobitidae: Cobitis) and their phylogenetic positions. Ichthyol. Res., 52: 111-122. https://doi.org/10.1007/s10228-004-0261-3
  20. Ko, M.H. 2009. Reproductive mechanisms of the unisexual diploid-triploid hybrid complex between the spined loach Cobitis hankugensis and Iksookimia longicorpa (Teleostei, Cobitidae) in Korea. Doctoral Thesis, Chonbuk National University, Jeonju, 160pp.(in Korean)
  21. Ko, M.H. and I.C. Bang. 2014. Spawning character and early life history of the endangered Korean dwarf loach, Kichulchoia brevifasciata (Teleostei: Cobitidae). Korean J. Ichthyol., 26: 89-98.(in Korean)
  22. Ko, M.H. and J.Y. Park. 2011. Growth and spawning ecology of Cobitis lutheri (Teleostei: Cobitidae) in the Mangyeong river, Korea. Korean J. Ichthyol., 23: 158-162.(in Korean)
  23. Ko, M.H. and J.Y. Park. 2012. Eggs development and early life history of spine loach, Cobitis hankugensis (Pisces: Cobitidae), endemic to Korea. Korean J. Ichthyol., 24: 94-100. (in Korean)
  24. Ko, M.H., J.Y. Park and I.S. Kim. 2009a. Development of eggs and early life history of Iksookimia longicorpa (Pisces: Cobitidae) from Nakdong River of Korea. Korean J. Ichthyol., 21: 15-22.(in Korean)
  25. Ko, M.H., J.Y. Park and S.H. Kim. 2009b. Habitat enviornment and feeding habitat of Iksookimia koreensis and Cobitis lutheri (Pisces: Cobitidae) in the Mangyeong River, Korea. Korean J. Ichthyol., 21: 253-261.(in Korean)
  26. Ko, M.H., S.Y. Park and I.C. Bang. 2012. Eggs development and early life history of spine loach, Iksookimia koreensis(Pisces: Cobitidae). Korean J. Limnol., 45: 93-101.(in Korean)
  27. Ko, M.H., S.Y. Park and I.C. Bang. 2013. Eggs development and early life history of endangered spine loach, Iksookimia pumila (Pisces: Cobitidae). Korean J. Ichthyol., 25: 65-73. (in Korean)
  28. Ko, M.H. and Y.J. Won. 2015. Egg development and early life history of the Korean spine loach, Cobitis tetralineata (Pisces: Cobitidae). Korean J. Ichthyol., 27: 95-103.(in Korean)
  29. Ko, M.H., Y.S. Jeon and Y.J. Won. 2017. Early life history of the eastern Korean tetraploid spined loach, Iksookimia yongdokensis (Pisces: Cobitidae). Folia Zool., 66: 153-162. https://doi.org/10.25225/fozo.v66.i3.a2.2017
  30. Kottelat, M. 2012. Conspectus Cobitidum: An inventory of the loaches of the World (Teleostei: Cypriniformes: Cobitoidei). Raffles Bull. Zool., Suppl. 26: 1-199.
  31. Kwan, Y.S., D. Kim, M.H. Ko, W.O. Lee and Y.J. Won. 2018. Multi-locus phylogenetic analyses support the monophyly and the Miocene diversification of Iksookimia (Teleostei: Cypriniformes: Cobitidae). Syst. Biol., 16: 81-88.
  32. Kwan, Y.S., M.H. Ko and Y.J. Won. 2014. Genomic replacement of native Cobitis nalbanti with introduced C. tetralineata through a hybrid swarm following the artificial connection of river systems. Ecol. Evol., doi: 10.1002/ece3.1027.
  33. Lee, W.O., K.H. Kim, J.M. Baek, Y.J. Kang, H.Z. Jeon and C.H. Kim. 2011. Embryonic development and early life history of the northern loach, Cobitis pacifica (Pisces: Cobitidae. Korean J. Limnol., 44: 1-8.(in Korean)
  34. Linnaeus, C. 1758. Systema Naturae per Regna Tria Naturae, Tomus I. Edito Decima, Reformata. Laurentius Salvius, Holmiae (Stockholm). 824pp.
  35. ME (Ministry of Environment). 2006. Technology for Utilization and Control of Ecosystem. Kunsan University, Kunsan, 537pp.
  36. ME(Ministry of Environment). 2009. Development of Genetic Diversity Analysis, Culture and Ecosystem Restoration Techniques for Endangered Fish, Iksookimia choii. Soonchunhyang University, Asan. 537pp.(in Korean)
  37. ME (Ministry of Environment). 2011. Culture and Restoration Research of Endangered Freshwater Fish (four species include Liobagrus obesus). Soonchunhyang University, Asan, 359pp. (in Korean)
  38. MLTM (Ministry of Land & Transport and Maritime Affairs). 2010. Culture and Restoration of Endangered Species in the Major Four River Drainages. Soonchunhyang University, Asan, 489pp.(in Korean)
  39. MLTM (Ministry of Land & Transport and Maritime Affairs). 2011 Culture and Restoration of Endangered Species in the Major Four River Drainages(II). Soonchunhyang University, Asan, 364pp.(in Korean)
  40. Mori, T. 1952. Check List of the Fishes in Korea. Mem. Hyogo Univ. Agr. 1 (3) Bio. ser. No. 1. 228pp.
  41. Nalbant, T.T. 1993. Some problems in the systematics of the genus Cobitis and its relative (Pisces: Ostariophysi, Cobitidae). Rev. Roum. Biol.(Biol. Anim.), 38: 101-110.
  42. Nelson, J.S., T.C. Grande and M.V.H. Wilson. 2016. Fishs of the World (Fifth edition). John Wiley & Sons, Hoboken, New Jersey, p. 190.
  43. NIBR (National Institute of Biological Resources). 2013. Breeding Manual of Endangered Freshwater Fish. Incheon, 239pp. (in Korean)
  44. Okada, Y. and R. Seishi. 1937. Morphological and ecological study on the larvae and juveniles of freshwater fishes in Japan (viii) Cobitis biwae Jordan and Snyder. Bull. Fish. Res., 32: 549-554.(in Japanese)
  45. Park, J.Y. and I.S. Kim. 1997. Egg membrane in five cobitid species of Cobitis (Pisces: Cobitidae). Korean J. Ichthyol., 9: 121-129.
  46. Perdices, A., V.P. Vasil'ev & E.D. Vasil'eva, 2012. Molecular phylogeny and intraspecific structure of loaches(genera Cobitis and Misgurnus from the Far East region of Russia and some conclusions on their systematics. Ichthyol. Res., 59: 113-123. https://doi.org/10.1007/s10228-011-0259-6
  47. Rendahl, H. 1935. Ein paar neie unteraten von Cobitis taenia. Mem. Soc. Fauna et Flora Fennica, 10: 329-336.
  48. Saitoh, K. and K. Hosoya. 1988. Cobitis biwae Jordan et Synder. Page 159 in M. Okiyama, ed. An Atlas of the Early Stage Fishes in Japan. Tokai Univ. Press, Tokyo, xii+1154pp. (in Japanese)
  49. Sakai, H., T. Yokoyama, Y. Sakaiet, S. Matsumoto and Z. Kubota. 1989. Notes on the life history of Cobitis takatsuensis, a spinous loach. Bull. Biogeogr. Soc. Jpn., 44: 39-47. (in Japanese)
  50. Shimizu, T., H. Sakai and N. Mizuno. 1998. Embryonic and larval development of a Japanese spinous loach, Cobitis takatsuensis. Ichthyol. Res., 45: 377-384. https://doi.org/10.1007/BF02725190
  51. Slechtova, V., J. Bohlen and H.H. Tan. 2008. Families of cobitoidea (teleostei; cypriniformes) as revealed from nuclear genetic data and the position of the mysterious genera Barbucca, Psilorhynchus, Serpenticobitis and Vaillantella. Mol. Phylogenet. Evol., 44: 1358-1365. https://doi.org/10.1016/j.ympev.2007.02.019
  52. Song, H.Y., H. Yang, E.M. Cho, H.C. Shin and I.C. Bang. 2009. Morphological development of egg and larvae of Koreocobitis naktongensis (Cobitidae). Korean J. Ichthyol., 21: 247-252. (in Korean)
  53. Song, H.Y., W.J. Kim, W.O. Lee and I.C. Bang. 2008. Morphological development of egg and larvae of Iksookimia choii (Cobitidae). Korean J. Limnol., 41: 104-110.(in Korean)
  54. Suzuki, R. 1966. Artficial spawning and early development of the loach, Cobitis delicata Niwa. Bull. Freshwater Fish. Res. Lab., 15: 175-188.(in Japanese)
  55. Tanaka, S. 1973. Significationce of Egg and Larval Surveys in the Studies of Population Dynamics of Fish. In: Blaxter, J.H.S. (ed.), The Early Life History of Fish. Springer-Verlag, pp. 151-157.
  56. Vasil'eva, E.D., D. Kim, V.P. Vasil'ev, D. Kim, V. Vasil'ev, M.H. Ko and Y.J. Won. 2016: Cobitis nalbanti, a new species of spined loach from South Korea, and redescription of Cobitis lutheri (Teleostei: Cobitidae). Zootaxa, 4208: 577-591. https://doi.org/10.11646/zootaxa.4208.6.5