• YAKHAK HOEJI
• /
• v.4 no.1
• /
• pp.53-55
• /
• 1959

In order to confirm on production of vitamin $B_{12}$ during the kimchi fermentation period, the variation of its content is studied in this paper As a sample of kimchi for this fermentation study due to the seasonal condition, nabakkimchi which is aseasonal one in early spring, is prepared by author. The content of vitamin $B_{12}$ is estimated by the microbiological assay method using lactobacillus leichmannii A Tee 7830. Details for assay are indicated in the original part. And the pseudo-vitamin $B_{12}$ substances as thymidine which is active to the growth of lactobacillus leichmannii, are eliminated by the alkali treatment method of sample solution, According to the results shown in table 2 and 3 and figures 1 to 4, the following conclusions are summerized; 1. Vitamin $B_{12}$ produced during the fermentation period of kimchi. And the content of this vitamin during its optimum fermentation period, is much higher content compared with the theoretically calculated amount from its materials, This has been confirmed at the repeated experiment. The trend of variation during its period is indicated at the figures. 2. The sterilized kimchi inhibited the fermentation by sterilization at the preparation, did not increased its content showing only its theoretically calculated amount.

• Journal of Sericultural and Entomological Science
• /
• v.44 no.2
• /
• pp.74-81
• /
• 2002

The optimal conditions for mycelial growth of P. linteus ASI 26011 were 25-30$^{\circ}C$ and pH 6.0, respectively. The mycelial growth of P. linteus was excellent on MCM medium. In case of carbon sources, the mycelial growth of P. linteus was best on the culture media that were contained with sucrose, mannose and glucose. Potassium nitrate and sodium nitrate were good for the mycelial growth of P. linteus as a nitrogen source. For comparison of the mycelial colonization of P. linteus on logs, several techniques of inoculation were tested; the sterilized short log inoculation, drilling inoculation and log-end sandwich inoculation. The mycelial colonization of P. linteus on logs was good in the treatment of sterilized short log inoculation, but poor in the traditional methods such as drilling inoculation and log-end sandwich. The initial mycelial growth and the full mycelial colonization of P. linteus were the best on 20 cm logs under the condition of 42% of moisture content in log. Also the initial mycelial growth of P. linteus was accelerated over 12 hours of sterilization. Burying method of logs after 5-6 months of incubation was the best for formation of basidiocarp of P. linteus. The formation of fruiting body of P. linteus was quite good in the cultivation house at the 31-35$^{\circ}C$ and over 96% of relative humidity.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.47 no.6
• /
• pp.453-458
• /
• 2002

High temperature sterilization method (30min. at $40^{\circ}C$longrightarrow30 min. at $70^{\circ}C$longrightarrow30 min. cooling at $20^{\circ}C$) was evaluated better than that of chloride, ion water, $O_3$ water, and salt water for the controling of micro-organisms at the early stage of sprout culture. Due to the improved germination rate and effective control of micro-organisms at early stage, the method resulted in much lower in the number of micro-organisms, higher in sprout yield, and 2 days longer in quality keeping at market than sprouts prepared by conventional method. There are two methods related with water temperature; constant temperature method (18.5$\pm$ $0.5^{\circ}C$) which keeps the same water temperature during the culture and 3-stage temperature method which changes the water temperature depending on the days after culture. Three-stage temperature method set the temperature at 21$\pm$ $1^{\circ}C$) in the second and third days after the initial acceleration of germination (about 4 hours), at 18.5$\pm$0.5$^{\circ}C$ in the fourth and fifth days, and at 17.0$\pm$ $0.5^{\circ}C$) in the sixth and seventh days. Three-stage temperature method could enhance the resistance power to diseases at the early growth stage, control the growth to standard goods (8-9cm in length and 2.15-2.30mm in width), and keep good body color at the middle or final stage. This method also increased the sprout yield by 6% compared with the conventional method, constant temperature method.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2012.08a
• /
• pp.270-270
• /
• 2012

Low temperature atmospheric pressure plasmas (APPs) have been known to be effective for living cell inactivation in the water [1]. Many earlier research found that pH level of the solution was changed from neutral to acidic after plasma treatment. The importance of the effect of acidity of the solution for cell treatments has already been reported by many experiments. In addition, several studies have demonstrated that the addition of a small amount of oxygen to pure helium results in higher sterilization efficiency of APPs [2]. However, it is not clear yet which species are key factors for the cell treatment. To find key factors, we used GMoo simulation. We elucidate the processes through which pH level in the solution is changed from neutral to acidic after plasma exposure and key components with pH and air variation with using GMoo simulation. First, pH level in a liquid solution is changed by He+ and He(21S) radicals. Second, O3 density decreases as pH level in the solution decreases and air concentration decreases. It can be a method of removing O3 that cause chest pain and damage lung tissue when the density is very high. H2O2, HO2 and NO radicals are found to be key factors for cell inactivation in the solution with pH and air variation.

• Korean Journal of Plant Resources
• /
• v.32 no.6
• /
• pp.730-734
• /
• 2019

Cryopreservation is one of the ideal and suitable methods for long-term storage of plant germplasm. The plant contaminated with diseases and pathogens are decreased the multiplication rate, survival rate and high quality of plants after cryopreservation. The aim of this work was to improve a micropropagation method for lily in Korea, which is indigenous plant. In the last process of rinsing scales after surface-sterilization, we tried to control the diseases and pathogens lived within the tissue by rinsing in 0.03% sodium hypochlorite (NaClO) instead of sterile distilled water. Bulb scales of Lilium were cultured in vitro on MS medium supplemented with Plant Preservative Mixture (PPM). The results showed that L. tsingtauense accessions were observed ranged from 53.9 to 100% with a mean value of 76.8% and L. hansonii accessions were checked from 84.5 to 85.5% with a mean of 85% survival rate. The newly small bulb formed from bulb-scales was transferred to MS medium. We checked the presence of microorganisms and survival rate after 3 weeks in culture after examination of bacterial incidences. The results indicated that the non-contamination rate were shown ranged from 75.0 to 94.1% with mean value of 83.2% in L. tsingtauense species, and that L. hansonii were observed 85.1 to 91.7% with mean value of 88.4%. This study will provide a valuable basis for establishment of effective axenic cultures for in vitro micropropagation of Korean native lily species.

• Korean Journal of Adult Nursing
• /
• v.14 no.1
• /
• pp.26-33
• /
• 2002

Purpose: This is a clinical experimental study aimed to provide basic data to ensure quality care. The purpose of this study is to increase nurses hand washing behavior and decrease respiratory isolation rate of MRSA. Method: This study was conducted in Kangnam St. Marys hospital. The subjects included 5 nurses working at the NS ICU and 165 specimen for nasal swabs and 46 specimen for sputum cultures from 106 patients admitted to the NS ICU. The data were collected from June to September 2000. Results: 1.The percentage of the nurses hand washing was 15.6percent but was improved to 46.5 percent after the educational intervention. In particular, hand washing behavior was increased in situations such as after suctioning, before parenteral nutrition, and after providing hygiene care. 2. Results of nasal swab showed that MRSA isolation rate was reduced from 42.8 percent before the education to 18.6percent after the education. The sputum culture results also showed that the MRSA rate was reduced from 40.7percent before the education to 34.6percent after the education. Conclusion: The findings showed the nurses' strict hand washing behavior and use of disposable gloves and paper towers as well as use of hand sterilization spray by visitors or families can decrease the MRSA isolation rate in the NS ICU patients. Since the NS ICU patients may have respiratory complications due to long-term hospitalization, the nurses regular hand washing is important enough to be emphasized. Future research should be focused on the impact of nurses' hand washing behavior on the incidence of pneumonia, an iatrogenic infection.

• Journal of environmental and Sanitary engineering
• /
• v.16 no.4
• /
• pp.9-20
• /
• 2001

The objective of this study was to improve sanitary conditions at the beauty shops by informing the owners and employees to be aware of the public health. The data were obtained by the Physical investigation (August 1 to 30, 2001) of the sanitary Problems at 112 beauty shops in Daegu Metrpolitan area and by the surveys from the employees and customers. By analysis these data with SAS/PC+(ver. 6.12) and $x^2-test$, we have obtained the following results. 1. The numbers of the beauty shop workers (67.86% of the shops) were two including the owner, and only 18.75% of the workers had the regular public health training session. Even at the training sessions, no public health specialist was available as an instructor. 2. Sanitary conditions of the floor (92.76%), washer (88.93%) and rest room (74.11%) at the beauty shops were satisfactory. However, only 3.57% of the beauty shop used the vacuum cleaner that would be far more effective than the bloom to remove hairs from the floor. 3. About 91.07% (102 shops) did not or poorly sterilize equipments, and 91.76% (103 shops) did not store the sterilized or non-sterilized equipments separately. The shops that had the ultraviolet sterilizer that is required by the public health regulation were 53.57% (60 shops), and only 2.67% (3shops) actually used it. 4. About 82.14% (72 shops) used Oxyclean and detergent for washing the fabrics such as towels and gowns. About 11.61% (13 shops) used only detergent and 3.57% (4 shops) used the boiling sterilization method. No shop used the customer gown once per customer, and only 2.68% (3 shops) washed the used gown daily.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.2
• /
• pp.209-216
• /
• 2014

The efficiency of gamma irradiation (0, 1, 5, 10, 15, 20, and 30 kGy) as a sterilization method of corn samples (30 g) artificially contaminated with Fusarium moniliforme stored at normal condition ($25^{\circ}C$ with approximate relative humidity (RH) of 55%) and optimal condition ($25^{\circ}C$ with a controlled RH of 97%) was studied. The results showed that the fungal growth and the amount of fumonisin were decreased as the dose of gamma irradiation increased. Gamma irradiation at 1-5 kGy treatment significantly inhibited the growth of F. moniliforme by 1-2 log reduction on corn samples (P < 0.05). Sublethal effect of gamma irradiation was observed at 10-20 kGy doses after storage, and a complete inactivation required 30 kGy. Fungal growth and fumonisin production increased with higher humidity and longer storage time in all corn samples. This study also demonstrated that there was no strict correlation between fungal growth and fumonisin production. Storage at normal condition significantly resulted in lower growth and fumonisin production of F. moniliforme as compared with those stored at optimal condition (P < 0.05). Gamma irradiation with the dose of ${\geq}5$ kGy followed by storage at normal condition successfully prolonged the shelf life of irradiated corns, intended for human and animal consumptions, up to 7 weeks.

• Korean Journal of Food Science and Technology
• /
• v.10 no.2
• /
• pp.224-230
• /
• 1978

Heat resistant putrefactive microorganisms causing spoilage of canned and processed foods were surveyed in the compost on mushroom growing bed, casing soil, raw mushrooms and canned products before sterilization at canneries located at 8 places including Buyo in Chung-Cheung-Do and monitored the total count and spore formers from the sample taken. The 9 strains of most severe heat resistant among the selected 140 spore formers were selected and determined D and Z value by TDT method. The most strong heat resistant strain was No. F-10, facultative thermophile, which was isolated from raw mushroom in Buyo area and it's Z value was $21.1^{\circ}F$ (M/15 phosphate buffer solution) and $D^{250}$ was 6.6 min.

• Korean Journal of Medicinal Crop Science
• /
• v.3 no.3
• /
• pp.259-264
• /
• 1995

This study was carried out to find cultivation condition of peony in Euiseong district from 1994 to 1995. 1. Age distribution in cultivation of peony was from 30's to 50's and cultivated years was mainly from 5 years to 10 years, but there was more than 15 years 2. Cultivating area per house hold were 1300 pyeong average, there were moderate $400{\sim]1000$ pyeong and also those are occupied 22.6% more than 2000 pyeong. 3. The mothods of transplanting in Euiseong peony which Euiseong district was mostly divided peony but Yeongchen district used seedling stock. No. of sprout was mostly $3{\sim}4$ and planting time was mainly from Mid to Late of October. 4. The percentage of sterilization of soil and seedling stock was 77. 4%, in mulching by vinyl in tran­splanting 64. 5% in black vinyl and 25. 8% in white one. 5. Number of plant in peony planting was $3000{\sim}4000$ plant per l0a even more there was more than 8000 plant. 6. Chemical spraying time in peony field was 2 time in $1{\sim}2$ years cultivating, and $3{\sim}4$ time in $3{\sim}4$ years one. 7. Condition of fertilizer application in peony field was 40% in non-application, but $56.7{\sim}76.7%$ in 2 year to 3 years, and the time of fertilizer was mainly 3 time. 8. Drying time peony after havesting was from 12 to 24 hours by briquet stove and 24 hours by ma­chine of hot wind, also dry method of peony was mixed briquet stove and hot wind machine.