• The Plant Pathology Journal
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• 제32권2호
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• pp.85-94
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• 2016

Studies were conducted to determine the role of 3-methylthioproprionic acid (MTPA) in the pathogenicity of potato stem canker, Rhizoctonia solani, and the concentrations required to inhibit growth of R. solani under laboratory and plant house-based conditions. The experiments were laid out in a completely randomized design with five treatments and five replications. The treatments were 0, 1, 2, 4, and 8 mM concentrations of MTPA. The purified toxin exhibited maximal activity at pH 2.5 and $30^{\circ}C$. MTPA at 1, 2, 4, and 8 mM levels reduced plant height, chlorophyll content, haulm fresh weight, number of stolons, canopy development, and tuber weight of potato plants, as compared to the control. MTPA significantly affected mycelial growth with 8 mM causing the highest infection. The potato seedlings treated with MTPA concentrations of 1.0-8.0 mM induced necrosis of up to 80% of root system area. Cankers were resulted from the injection of potato seedling stems with 8.0 mM MTPA. The results showed the disappearance of cell membrane, rough mitochondrial and cell walls, change of the shape of chloroplasts, and swollen endoplasmic reticulum. Seventy-six (76) hours after toxin treatment, cell contents were completely broken, cytoplasm dissolved, and more chromatin were seen in the nucleus. The results suggested that high levels of the toxin concentration caused cell membrane and cytoplasm fracture. The integrity of cellular structure was destroyed by the phytotoxin. The concentrations of the phytotoxin were significantly correlated with pathogenicity and caused damage to the cell membrane of potato stem base tissue.

• Molecules and Cells
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• 제37권12호
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• pp.907-911
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• 2014

The function of reactive oxygen species (ROS) as second messengers in cell differentiation has been demonstrated only for a limited number of cell types. Here, we used a well-established protocol for BMP2-induced neuronal differentiation of neural crest stem cells (NCSCs) to examine the function of BMP2-induced ROS during the process. We first show that BMP2 indeed induces ROS generation in NCSCs and that blocking ROS generation by pretreatment of cells with diphenyleneiodonium (DPI) as NADPH oxidase (Nox) inhibitor inhibits neuronal differentiation. Among the ROS-generating Nox isozymes, only Nox4 was expressed at a detectable level in NCSCs. Nox4 appears to be critical for survival of NCSCs at least in vitro as down-regulation by RNA interference led to apoptotic response from NCSCs. Interestingly, development of neural crest-derived peripheral neural structures in Nox4-/- mouse appears to be grossly normal, although Nox4-/- embryos were born at a sub-Mendelian ratio and showed delayed over-all development. Specifically, cranial and dorsal root ganglia, derived from NCSCs, were clearly present in Nox4-/- embryo at embryonic days (E) 9.5 and 10.5. These results suggest that Nox4-mediated ROS generation likely plays important role in fate determination and differentiation of NCSCs, but other Nox isozymes play redundant function during embryogenesis.

• 한국자원식물학회지
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• 제18권2호
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• pp.246-250
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• 2005

아시아 지역에서 수집한 야생 쓴메밀의 종자 및 식물체 내의 루틴함량을 조사하였다. 쓴메밀은 종자 및 식물 전체부위에서 숙근메밀이나 단메밀보다 높은 루틴 함량을 나타냈다. 특히, 꽃은 숙근메밀의 2.2배, 단메밀의 9.5배, 줄기는 각각 3.1배와 24.9배, 종자는 3.2와 65배나 높았다. 3종에 대한 식물체 부위별 루틴함량은 모두 꽃>잎>종자>줄기>뿌리의 순으로 높았다. 종피색에 따른 루틴함량은 암회색>검은색>갈색, 회갈색 순의 경향을 나타냈으며, 종자의 형태는 Slender>Notched>Round 순으로 루틴함량이 높았다. 재배종은 야생종에 비해 루틴함량이 잎은 1.3배, 줄기가 1.4배, 종자가 1.2배의 높은 경향을 보였다. 부탄지역 수집품종은 잎, 줄기, 종자의 루틴함량이 다른 지역보다 높은 경향을 보였고, 슬로베니아 및 파키스탄지역 수집품종은 잎, 줄기에서, 인도 및 네팔지역 수집품종은 종자에서 가장 낮은 루틴함량의 경향을 보였다.

• Journal of Ginseng Research
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• 제6권1호
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• pp.38-45
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• 1982

To determine the optimum light intensity for growth of ginseng plants, change of temperature, moisture content in son, occurrence alternaria blight, defoliation rate, chlorophyll contents, and growth of shoots and roots were investigated under different light intensity such as 5%, 10%, 20% and 30% light transmittance rare(L.T.R.). The results obtained were as follows. 1. Maximum temperature under the shading was increased as the increase of light intensity, whereas soil moisture content decreased 2. As the increase of light intensity, stem and Peduncle length, leaf area, and chlorophyll contents decreased significantly but length and width of the leaf was not significant, while stem diameter, special leaf weight and chlorophyll a/chl. b ratio increased 3. Stem color was shown dark purp!e as the increase of light intensity. 4. Photosynthesis during the day was highest at 9 A.M. and decreased as time passed in all plots. The means of photouynthesis during the day showed in the order of 20%, 10%, 30%, 5% L.T.R., and optimum light intensity for highest photosxthesis was 18.4% L.T.R. by theoritical equation. 5. It was showed a tendency that alternaria leaf blight of ginseng plants was increased as the increase of light intensity. 6. Defoliation rate of ginseng plants was increased as the increase of light intensity, especially all plants were defoliated by late June without shading. 7. Yield percentage of the rear line was increased as the increase of light intensity. Root weight per plant showed in the order of 20%, 10%, 30%, 5% L.T.R., and optimum light intensity for the best yield was 18.5% L.T.R. by theeritical equation.

• Journal of Plant Biotechnology
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• 제41권4호
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• pp.236-241
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• 2014

An efficient regeneration protocol for stem disc culture of Populus euramericana, which is important species for bioenergy resource in agroforestry, was established. The number of explants that were obtained and the number of explants that regenerated varied with the genotypes. However, in all the genotypes, stem disc culture produced more regenerated shoots than did in axillary bud culture. A comparison of the effects of cytokinin type and concentration on shoot regeneration in different explants (i.e., petiole, leaf, and root segments of P. euramericana) revealed that a concentration of $0.002mg\;l^{-1}$ thidiazuron (TDZ) used on petiole segments resulted in the greatest shoot regeneration (95.83%). The hormonal requirements for the greatest shoot regeneration in the three explant types varied. Different concentrations of $AgNO_3$ and $CoCl_2$ were added separately to the medium to stop the yellowing and subsequent necrosis of the regenerated shoots. Lower concentrations (3 and $5mg\;l^{-1}$) of these compounds improved shoot regeneration and elongation, compared with the control. The in vitro-regenerated shoots were transferred to rooting medium and subsequently acclimatized. The highly efficient regeneration system of P. euramericana reported here can be used for mass propagation of this recalcitrant for regeneration, economically important tree species.

• 한국산림과학회지
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• 제32권1호
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• pp.1-8
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• 1976

잣나무 2-1묘(苗)와 방크스소나무 1-1묘(苗)의 생장(生長)에 미치는 밀도효과(密度効果)를 보기 위하여 광릉묘포(光陵苗圃)에서 잣나무의 경우는 $m^2$당 36본에서 324본까지 식재(植栽)하였으며 방크스소나무는 25본에서 169본까지 식재(植栽) 하였다. 실험결과(實驗結果) 묘고(苗高), 근원경(根元經), 건중량(乾重量) 및 규격묘(規格苗) 생산(生産)에 뚜렷한 효과(効果)를 보았다. 잣나무 2-1묘(苗)는 밀식(密植)시킬수록 수고생장(樹高生長)은 증대되고, 방크스소나무는 식재밀도구간(植栽密度區間)에 차이(差異)가 없었다. 잣나무와 방크스소나무는 밀식시킬수록 근원경(根元經) 생장(生長)은 감소되는 경향이 있으나 후자(後者)가 급격히 감소하고 있다. 잣나무의 평균목(平均木) 건중량(乾重量)은 밀식(密植)시킬수록 감소율은 낮으나 방크스소나무는 높다. 밀식(密植)으로 인해 일반적으로 T/R율(率)의 값은 증대되나 식재밀도구간(植栽密度區間)에 큰 차이(差異)가 나타나지 않고 있다. 평균목(平均木)의 잎 뿌리와 줄기의 건중량은 밀식(密植)시킬수록 감소되고 있으나 방크스소나무가 잣나무 보다 감소율이 높다. 그리고 잣나무의 경우는 줄기의 건중량(乾重量)은 밀식구(密植區)와 소식구간(疎植區間)에 차(差)가 적다. 밀식(密植)으로 나타나는 잣나무의 세장성(細長性) 보다는 방크스소나무의 세장성(細長性)이 더욱 높게 나타나고 있으나 밀식(密植)시킬수록 전체적인 물질생산량(物質生産量)은 증대되리라 생각 된다. 이상의 생장특성(生長特性)으로보아 잣나무의 이식상(移植床)을 만들때와 조림(造林)은 밀식(密植)시키도록 하고 방크스소나무는 소식(疎植)함이 효과(効果)적일것으로 생각 된다. 잣나무 2-1묘(苗)의 규격(規格)은 간장(幹長) 18cm 근원경(根元經)은 4mm로 개정(改正)시키고 $m^2$당 이식본수(移植本數)는 묘포비옥도(苗圃肥沃度)에 따라 160~200본(本)으로 함이 적합하리라 생각되며, 방크스소나무 1-1묘(苗)의 규격(規格)은 간장(幹長) 25cm 근원경(根元經) 6mm가 적합하며 $m^2$당 이식본수(移植本數)는 묘포비옥도(苗圃肥沃度)에 따라 100-120본(本)이 적합할 것이다.

• 한국유기농업학회지
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• 제22권2호
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• pp.321-334
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• 2014

국내 식물자원 및 약용식물들의 식물 생장촉진 효과를 조사하기 위하여 64종 65점의 추출물 시료를 조제하여 벼(Oryza sativa) 유묘 생장에 미치는 영향을 평가하였다. 그 결과, 11종 12점의 쑥(Artemisia princeps) 경엽, 엉겅퀴(Cirsium japonicum var. maackii) 전초, 진달래(Rhododendron mucronulatum) 가지와 잎, 갈대(Phragmites communis) 줄기, 율무(Coix lacrymajobi var. mayuen) 전초, 싸리(Lespedeza bicolor) 가지와 잎, 산수국(Hydrangea serrata f. acuminata) 전초, 속단(Phlomis umbrosa) 전초, 감초(Glycyrrhiza uralensis) 근경, 감초(G. uralensis) 경엽, 당귀(Angelica gigas) 뿌리 및 천궁(Cnidium officinale) 근경 추출물은 벼 유묘 생장을 촉진시키는 것으로 나타났다. 본 결과에 따라 상기 식물 종의 부위들은 식물 생장촉진용 농가 자가제조 액비 개발을 위한 소재로써 활용가능성이 있는 것으로 판명되었다.

• 대한한의학회지
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• 제21권2호
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• pp.52-59
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• 2000

Objectives: The root and stem of Sinomenium acutum has been used for treatment of arthritis and neuralgia in oriental medicine. To find new substances of the anti-anaphylactic drugs, we studied Sinomenium acutum. Methods: To investigate the effect of this plant, the effect on anaphylactic reaction, plasma histamine level, and tumor necrosis $factor-{\alpha}-(TNF-{\alpha})$ production were measured after the aqueous extract of Sinomenium acutum stem (SSAE) was administrated to mice and rats. Results: The SSAE (0.1 to 1000 mg/kg) dose-dependently inhibited systemic anaphylactic reaction induced by compound 48/80 in mice. Especially, SSAE reduced compound 48/80-induced anaphylactic reaction with 50% at the dose of 1000 mg/kg. SSAE (100 to 1000 mg/kg) also significantly inhibited local anaphylactic reaction activated by anti-dinitrophenyl (DNP) IgE. When mice were pretreated with SSAE at a concentration ranging from 0.1 to 1000 mg/kg, the plasma histamine levels were reduced in a dose-dependent manner. SSAE (1 to 1000 g/ml) dose-dependently inhibited histamine release from the rat peritoneal mast cells (RPMCs) activated by compound 48/80 or anti-DNP IgE. The level of cAMP in RPMCs, when SSAE was added, increased compared with that of a normal control. In addition, SSAE (0.1 g/ml) had a significant inhibitory effect on anti-DNP IgE-induced $TNF-{\alpha}$ production. Conclusions: These results indicate that SSAE inhibits mast cell-mediated anaphylactic reactions and $TNF-{\alpha}$ production from mast cells.

• 한국산림과학회지
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• 제94권6호
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• pp.441-445
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• 2005

기후변화 협약과 관련된 교토 의정서를 계기로 산림의 탄소 축적량을 조사하기 위하여 전국 규모의 산림 현존량 추정에 대한 연구가 진행되고 있다. 전국 규모의 산림 현존량 추정은 일반적으로 임업통계상의 임목축적 자료를 이용하고 있으며, 산림 현존량은 줄기의 건중량 대 재적 비인 줄기밀도와 임목 전체 건중량 대 줄기 건중량 비인 현존량확장계수에 의하여 산출할 수 있다. 본 연구는 한국 소나무의 대표적 생태형인 금강형 소나무와 중부지방 소나무를 대상으로 임령에 따른 줄기밀도와 현존량확장계수를 조사분석함으로써 임목축적에 의한 현존량 추정에 대한 정보를 제공하는데 목적이 있다. 금강형 소나무와 중부지방 소나무는 모두 영급이 증가함에 따라 줄기밀도가 증가하였으며, 현존량확장계수는 감소하였다. 금강형 소나무 줄기밀도의 경우 20년생 이하 영급과 40-60년생 영급 간에는 유의적인 차이가 있었으며, 중부지방 소나무의 뿌리를 제외한 지상부 현존량확장계수의 경우 20년생 이하, 20-40년생, 40-60년생 영급간에 모두 유의적인 차이가 있었다. 동일 영급에서 금강형 소나무는 중부지방 소나무에 비하여 줄기밀도와 현존량확장계수가 모두 낮은 값을 보였다. 20년생 이하와 20-40년생 영급의 경우 줄기밀도와 현존량확장계수 모두 금강형 소나무와 중부지방 소나무간에 유의적인 차이가 있었다. 이상을 종합하면, 임업통계상의 임목축적을 이용하여 소나무림의 현존량을 추정할 경우 전환계수인 줄기밀도와 현존량확장계수는 금강형 소나무와 중부지방 소나무를 구분하고 적정 범위의 영급에 따라 달리 적용함으로써 정확도를 높일 수 있는 것으로 나타났다.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1974년도 학술대회지
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• pp.101-113
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• 1974

The radioactive compound sodium $acetate-U-C^{14}$ (C-14 acetate) was administered to two- and four-year-old July and September American ginseng (Panax quinquefolium L.) plants and cuttings. The C-14 acetate uptake was approximately $99\%.$ The autoradiochromatograms suggest that the saponins(panaquilins) isolated by preparative thin-layer chromatography contained impurities, especially those isolated from the leaf and stem extracts. The root and fruit methanol extracts yielded relatively pure saponins. The large amounts of panaquilin B and its proximity to panaquilin C on preparative thin-layer plates resulted in some admixing. The average concentration $(\%$ plant dry weight) of semipurified saponins were high in the leaves $(13.8\%),$ compared to fruits $(9.8\%),\;stems\;(7.9\%)\;and\;roots\;(6.3\%).$ The average percentage of C-14 acetate incorporation into panaquilins was $4.8\%.$ The average percentage of C-14 acetate incorporation into panaquilins B and C was higher $(1.40\%\;and\;1.13\%,$ respectively) than that into panaquilin C, (d), G-1 and G-2 $(0.75\%,\;0.65\%,\;0.13\%\;and\;0.53\%,$ respectively). Panaquilin synthesis may be depending upon the part collection period and age of the plant. The average percentage of C-14 acetate incorporation into panaquilin B is high in roots $(0.58\%)\;and\;stems\;(0.48\%);$ that into panaquilins C and (d) high in leaves $(0.40\%\;and\;0.45\%,$ respectively); and that into panaquilin E high in roots and leaves $(0.55\%\and\;0.50\%,$ respectively). Panaquilin G-2 was synthesized in all parts of plants. The panaquilins appear to be biosynthesized more actively in July than September (exception-panaquilin G-l). Panaquilins B, C and G-1 may be biosynthesized more actively in four-year-old plants and panaquilins (d) and E more actively in two-year-old plants. The results from expectance with cuttings suggest that the panaquilins are synthesized de novo in the above-ground parts of ginseng plants, and that panaquilin G-l may be synthesized de novo in the leaf. It is known from the tissue culture studies that panaquilins are produced by leaf, stem and root callus tissues and callus-root cultures of American and Korean ginseng plants. Panaquilins may actively be synthesized de novo in most any cell or organ of the ginseng plants. It was verified that C-14 acetate was incorporated into the panaxadiol portions of the panaquilins of two-year-old plants (sp. act., 0.56 $m{\mu}Ci/mg$) and four-year-old plants (sp. act., 0.54 $m{\mu}Ci/mg$).