• Title/Summary/Keyword: stem and root

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Assessment on Antioxidant Potential and Enzyme Activity of Some Economic Resource Plants

  • Boo, Hee-Ock;Shin, Jeoung-Hwa;Shin, Ji-San;Choung, Eui-Su;Bang, Mi-Ae;Choi, Kyung-Min;Song, Won-Seob
    • Korean Journal of Plant Resources
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    • v.25 no.3
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    • pp.349-356
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    • 2012
  • The antioxidant potential and enzyme activities in Salicornia herbacea, Corylopsis coreana, Erythronium japonicum, Phragmites communis, Momordica Charantia, Nelumbo nucifera, Salvia plebeia, Portulaca oleracea, Ficus carica, Citrus junos and Cornus officinalis were determined. Their antioxidant activities were measured using DPPH radical scavenging and nitrite scavenging activity. Enzyme activities in investigated plants were evaluated as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX). The DPPH scavenging rate from 100 to 2500 $mgL^{-1}$ was the highest in the flower of Corylopsis coreana. However, it was not detected in most of the samples at concentration below 100 $mgL^{-1}$. The nitrite scavenging activity according to each kind of resource plants was significantly higher in the stem of Corylopsis coreana and leaf of Nelumbo nucifera. The root extract of Erythronium japonicum had the highest SOD enzyme activity of 94.0% while leaf of Salvia plebeia showed the lowest SOD enzyme activity of 30.4%. The activity of CAT and APX showed higher values in the stem of Corylopsis coreana, root of Erythronium japonicum and root of Phragmites communis in comparison with other plants. The activity of POD showed significantly high values in stem of Corylopsis coreana, Momordica Charantia and pericarp of Citrus junos extracts. The antioxidant enzyme activities differ significantly in different plants. In conclusion, we showed that Corylopsis coreana, Erythronium japonicum Cornus officinalis, and Momordica Charantia had the potent biological activities. Therefore, these plant resources showing antioxidant activity could be good materials for development of source of functional healthy food.

A study on the effect comparative of acanthopanax stem bark (ASB) and acanthopanax root bark (ARB) on the monosodium iodoacetate (MIA)-induced osteoarthritis rats (오가피(五加皮) 수피(樹皮)와 근피(根皮)의 MIA 유도 골관절염 흰쥐에 미치는 영향 비교)

  • Sim, Woo-Hyung;Seo, Bu-Il
    • The Korea Journal of Herbology
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    • v.33 no.5
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    • pp.53-66
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    • 2018
  • Objectives : This study was designed to compare the effects of acanthopanax stem bark (ASB) and acanthopanax root bark (ARB) on the monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods : The antioxidant activities were evaluated through radical scavenging assays using 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. Also, we examined total poly phenol and flavonoids contents. Osteoarthritis was caused by injection MIA($50{\mu}{\ell}$ with $80mg/m{\ell}$) into the knee joint cavity of rats. Rats were divided by 4 groups (normal group, control group, ASB treated group, ARB treated group, each n=6). The changes in the levels of reactive oxygen species (ROS), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum were analyzed after experiment. Also, the anti-oxidant, inflammatory protein levels were investigated western blot analysis. Knee joint tissue, histopathological observation hematoxylin & eosin staining and safranin-O staining were measured. Results : In the present study, ARB treated group showed superior inhibitory effects on the inflammatory parameters than the ASB treated group. ARB aqueous extract was effective in antioxidant measurements. The administration of ARB showed a significant reduction of changes in relative hind paw weight distribution. Morever, it decreased ROS, ALT and AST levels in serum, compared with those of the control rats. The ARB administration inhibited the biomarkers of inflammatory in tissues. Conclusions : ASB aqueous extract and ARB aqueous extract have a great effect on osteoarthritis, and ARB aqueous extract has excellent effect on osteoarthritis through antioxidant and anti-inflammation.

Effect of soil moisture on growth of P. ginseng (토양수분(土壤水分)이 인삼생육(人蔘生育)에 미치는 영향(影響))

  • Nam, Ki-Yeul;Park, Hoon;Lee, Il-Ho
    • Korean Journal of Soil Science and Fertilizer
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    • v.13 no.2
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    • pp.71-76
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    • 1980
  • Effect of soil moisture on growth of 2 year old Pan ax ginseng was investigated by pot culture using clay loam and sandy loam soils. In clay loam soil emergence rate of new shoot and stem growth were greatly decreased below 40% of field capacity. In sandy loam soil the growth of stem and leaf were greatly affected below 40% moisture. Root yield showed maximum at 60% moisture and significantly coorelated with stem diameter and root length. Rotten root rate and die back rate of top were least at 60% and gradually increased with either increase or decrease of soil moisture.

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Chemical Components in Different Parts of Korean Sword Bean(Canavalia gladiata) (한국산 작두콩의 부위별 화학성분)

  • 조영수;배영일;심기환
    • Food Science and Preservation
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    • v.6 no.4
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    • pp.475-480
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    • 1999
  • The chemical components in different parts such as seed, cotyledon, hull, pod, leaf, stem and root of Korean sword bean(Canavalia gladiata) were determined in this study. The contents of total sugar and protein were relatively higher in cotyledon than other parts. The highest mineral component was K, Ca in root and leaf, respectively. In case of organic acids, relatively higher content of oxalic acid(2,556 mg%), citric acid(573 mg%) was found in leaf and pod, respectively. Among free sugars, the contents of sucrose(3.80%), fructose(2.17%) were the highest contents in hull and stem, respectively. The highest component total amino acids in cotyledon and leaf, pod, stem, and root was glutamic acid(592 mg%), glutamic acid(429 mg%), proline(497 mg%), lysine(328 mg%) and arginine(714 mg%), respectively. Among fatty acids in hull and pod, palmitic acid(32.75%, 21.93%) was high in saturated fatty acid fraction, while linoleic acid(39.15%, 43.03%) was high in polyunsaturated fatty acid fraction.

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Pyrolysis Characteristics of Hemp By-products (Stem, Root and Bast) (헴프 부산물의 열분해 특성 연구)

  • Choi, Gyeong-Ho;Kim, Seung-Soo;Kim, Jinsoo;Joo, Dong-Sik;Lee, Janggook
    • Applied Chemistry for Engineering
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    • v.22 no.5
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    • pp.508-513
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    • 2011
  • Hemp is known as one of the most productive and useful plants, which grows quickly in a moderate climate with only moderate water and fertilizer. Traditionally in Korea, hemp bast is used to natural fibres, and remaining such as stem and root is treated as waste. Those of hemp by-products can be transformed to bio fuel such as bio-oil and activated carbon. To understand pyrolysis characteristics, thermogravimetric analysis were carried out in TGA, in which hemp by-products were mostly decomposed at the temperature range of $270{\sim}370^{\circ}C$. The corresponding kinetic parameters including activation energy and pre-exponential factor were determined by differential method over the degree of conversions. The values of activation energies for pyrolysis were increased as the conversion increased from 10 to 90%.

Nitrate enhances the secondary growth of storage roots in Panax ginseng

  • Kyoung Rok Geem ;Jaewook Kim ;Wonsil Bae ;Moo-Geun Jee ;Jin Yu ;Inbae Jang;Dong-Yun Lee ;Chang Pyo Hong ;Donghwan Shim;Hojin Ryu
    • Journal of Ginseng Research
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    • v.47 no.3
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    • pp.469-478
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    • 2023
  • Background: Nitrogen (N) is an essential macronutrient for plant growth and development. To support agricultural production and enhance crop yield, two major N sources, nitrate and ammonium, are applied as fertilizers to the soil. Although many studies have been conducted on N uptake and signal transduction, the molecular genetic mechanisms of N-mediated physiological roles, such as the secondary growth of storage roots, remain largely unknown. Methods: One-year-old P. ginseng seedlings treated with KNO3 were analyzed for the secondary growth of storage roots. The histological paraffin sections were subjected to bright and polarized light microscopic analysis. Genome-wide RNA-seq and network analysis were carried out to dissect the molecular mechanism of nitrate-mediated promotion of ginseng storage root thickening. Results: Here, we report the positive effects of nitrate on storage root secondary growth in Panax ginseng. Exogenous nitrate supply to ginseng seedlings significantly increased the root secondary growth. Histological analysis indicated that the enhancement of root secondary growth could be attributed to the increase in cambium stem cell activity and the subsequent differentiation of cambium-derived storage parenchymal cells. RNA-seq and gene set enrichment analysis (GSEA) revealed that the formation of a transcriptional network comprising auxin, brassinosteroid (BR)-, ethylene-, and jasmonic acid (JA)-related genes mainly contributed to the secondary growth of ginseng storage roots. In addition, increased proliferation of cambium stem cells by a N-rich source inhibited the accumulation of starch granules in storage parenchymal cells. Conclusion: Thus, through the integration of bioinformatic and histological tissue analyses, we demonstrate that nitrate assimilation and signaling pathways are integrated into key biological processes that promote the secondary growth of P. ginseng storage roots.

ASSAY OF POTENTIAL ANTIMUTAGENICITY OF ETHNIC MEDICINAL PLANT EXTRACTS OF PAPUA NEW GUINEA BY USING SOS CHROMOTEST (E. coLi PQ 37)

  • K. Sundarrao;Jeho Yum;Chang, Il-Moo
    • Toxicological Research
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    • v.7 no.2
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    • pp.151-155
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    • 1991
  • Thirty six crude drug samples have been prepared from different parts of twenty five plants belonging to different families, and antimutangenic activities were studied by using SOS chromotest (E. coli PQ 37). The following crude extracts of PNG medicinal plants which had a appreciable antimutagenic activity against mitomycin C were: Artocarpus communis (stem bark), Cycas circinalis (leaves), Merremia peltata (leaves), Intsia palembanica (leaves), Annona muricata (stem bark), and Artocarpus altilis (root bark).

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Study on the Elimination of Heavy Metal Pollutants in the Soil by Vascular Plant in the River Area (하천유역의 관속식물을 이용한 토양내의 중금속 오염원 제거에 관한 연구)

  • 강경홍;김인성
    • Journal of Korea Soil Environment Society
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    • v.1 no.2
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    • pp.23-34
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    • 1996
  • The biological magnification of Pb, Cu, Zn and Fe in vascular plant, Persicaria thunbergii, Trapa japonica and Nymphoides peltata natually grown at the river and riverside and relationship between the concentration of heavy metals in these vascular plants and in the soil were investigated in the Chonju river, Chonju city. The biological magnification values of these metals in P thunbergii were founded as follows ; Pb, Cu, Zn and Fe were 80.4~254.6$mu\textrm{g}$/g, 284.6~688.4$mu\textrm{g}$/g, 635.5~1979.4$mu\textrm{g}$/g and 1160.0~3590.9$mu\textrm{g}$/g respectively. In the case of T. japonica, Pb, Cu, Zn and Fe were 107.8~306.0$mu\textrm{g}$/g, 282.7~963.0p$mu\textrm{g}$/g, 1328.3~3546.$mu\textrm{g}$/g and 656.8~9944.0$mu\textrm{g}$/g and in N. peltata, 140.1~ 193.9$mu\textrm{g}$/g, 187.7~327.3$mu\textrm{g}$/g, 1126.6~1723.6$mu\textrm{g}$/g and 611.7~1914.6$mu\textrm{g}$/g respectively. The amount of the biological magnification of heavy metals in the leaf, stem and root of these plants was generally increased in the sequence of leaf

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Eleutheroside E Content in Eleutherococcus spp. (오갈피속(屬) 식물(植物)의 Eleutheroside E 함양(含量))

  • Kim, Young-Jin;Park, Moon-Soo;Park, Ho-Ki;Kim, Sun;Sung, Chung-Ki
    • Korean Journal of Medicinal Crop Science
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    • v.4 no.4
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    • pp.333-339
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    • 1996
  • The root and stem bark of Eleutherococcus spp. is used for the treatment of paralysis, rheumatism, arthritis, diabetes, stamina failure, high blood pressure, etc. This study was con­ducted to identify botanical origins and to select standard variety of Eleutherococcus spp. with analyzing eleutheroside E content(%) of root bark and that of Eleutherococcus senticosus at different collected area, time, part and grown area. The content of eleutheroside E in the root bark was determined by HPLC, and it was 0.1925% in Eleutherococcus senticosus, 0.1103% in E. chiisanensis, 0.0348% in E. koreanum and 0.0236% in E. sieboldianum. In seasonal change of eleutheroside E content, it was higher in autumn product than summer product in Eleutherococcus senticosus. It also was higher in root bark than stem bark in different part. To investigate eleutheroside E content of root bark among different collected local area in E. sen­ticosus, it was high order to Mt. Deokyu, Hokkaido and Mt. Seolak as a 0.1625%. 0.0493% and 0.027 %, respectively. The growth of Eleutherococcus senticosus was good at grown Unbong (altitude 10m), but eleu­theroside E content was high at grown Nogodan (altitude 1, 450m).

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