• 한국동물위생학회지
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• 제34권2호
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• pp.159-166
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• 2011

This study was carried out to develope enzyme-linked immunosorbent assay (ELISA) for detection of canine brucellosis in dogs experimentally inoculated with Brucella abortus 1119-3 and B. canis RM666. Groups A, B and C of dogs (each group consisting of three dogs) were orally inoculated with approximately $5{\times}10^9$ colony-forming units of B. abortus and B. canis, and with sterile pyrogen-free PBS, respectively. The animals were monitored at regular intervals upto the 12th week post inoculation (PI) by standard tube agglutination test (STAT), plate agglutination test (PAT), Rose Bengal test (RBT), 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and ELISA. The induced antibody titers in group A dogs were detected from the first week PI to the eighth week PI in STAT, PAT and RBT using the inactivated whole cells of B. abortus 1119-3 as antigens, while no sera in groups B and C dogs reacted with the antigens. In 2ME-RSAT using whole cells of B. canis M-strain as antigens, the induced antibody titers in group B dogs were observed at the second week PI and persisted for the 12th week PI, while sera of groups A and C dogs did not react with the whole cells. In ELISA using cytoplasmic fractions antigen of B. abortus 1119-3, the mean optical density of antibodies in groups A and B was detected from the first and second weeks PI, respectively, and persisted for 12th week PI, while sera of group C did not cross-react with the fractions antigen. However, in ELISA using the hot saline extracts of B. canis M- as an antigen, the induced antibody titers in only group B dogs were detected from second week PI and persisted for until the end of this study. These results indicate that the ELISA using B. abortus 1119-3 cytoplasmic fractions as antigens can be a good candidate for detection of brucellosis by B. abortus as well as B. canis in dogs.

• 한국동물위생학회지
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• 제33권1호
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• pp.29-35
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• 2010

This study was performed to test the hypothesis that Brucella abortus strain RB51 (SRB51) might protect Korean indigenous mongrel dog against challenge with either virulent B. abortus biotype 1 or B. canis. A total of 12 Korean mongrel dogs were divided into four groups (Group A, B, C and D). Dogs belonging to Group A and C were inoculated subcutaneously with $1{\times}10^9$ CFU of SRB51 in 1ml of sterile phosphate buffered saline (PBS). Dogs of Group B and D were inoculated subcutaneously with 1ml of sterile PBS as control. At 12 weeks post vaccination, dogs of Group A and B were challenged by oral inoculation of virulent strain of B. canis ($5.0{\times}10^9$ CFU) and dogs of Group C and D were challenged by oral inoculation of virulent strain of B. abortus biotype 1 ($4.4{\times}10^{10}$ CFU). The serum antibodies titers in all dogs were monitored at regular interval for eight weeks after challenge (AC) by standard tube agglutination test, plate agglutination test, rose bengal test, 2-mercaptoethanol rapid slide agglutination test and 2-mercaptoethanol tube agglutination test. No antibody titers in Group A and C was detected. Also, the challenge strains were not found from blood of all dogs of Group A and C from 1 week AC till the end of the experiment by culture and modified AMOS-PCR, whereas B. canis and B. abortus challenge strains were detected from blood of Group B and D, respectively. In addition, neither of two challenge bacteria was recovered from liver, spleen, kidneys, lymph nodes and reproductive tracts of Group A and C dogs after postmortem. However, B. canis and B. abortus challenge strains were isolated from these tissues of Group B and D, respectively. These data suggest that SRB51 could be a promising vaccine candidate for immunizing dogs to control canine brucellosis caused by B. canis or B. abortus.

• 한국동물위생학회지
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• 제30권3호
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• pp.375-384
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• 2007

This study was conducted to investigate the characteristics of brucellosis in Korean native cattle in a farm where bovine brucellosis was confirmed 3 times from September 2006 to March 2007. Of 74 bulls serum samples examined, 21 (28.4%) were positive by Rose-Bengal test (RBT) and Standard tube agglutination test (STAT). In the isolation test from seropositive bulls, B abortus was isolated and identified from 2 specimens (testis, intestinal lymph node) among 6 kinds of specimens including blood, urine, feces and soil. Isolation rate of intestinal lymph node and testis was 25% (3/12 cases) and 16.7% (2/12), respectively. B abortus was also isolated from calves below 12 months old, i.e., 1 isolate (25.0%) was confirmed from testis, 4 (40.0%) from supra-mammary lymph nodes and 1 (25.0%) from intestinal lymph node. All isolates had Brucella specific 16s r-RNA with 905-bp band detected by PCR assay. For the more effective control of bovine brucellosis in korea, this paper would like to suggest that all of bulls and calves should be included in the screening tests.

• 한국건강관리협회지
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• 제2권2호
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• pp.187-197
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• 2004

Owing to continuous economic growth, an increased standard of living, and improvements to health care in Korea, the life expectancy of Koreans rose from 69.0 years in 1985 to 71.3years in 1990. It continued to rise throughout the 1990s, and, by 2002, had reached 76.5years for the general population and 80.0 years for Korean females. Similarly, the percentage of the Korean population that is 65 years of age or older increased from 3.1 in 1970 to 7.1in 2000, and is expected to reach approximately 14,0 in 2019. Thus, according to this estimate, Korea will have evolved from an "aging society" to an "aged society" in only 19years. In the case of other countries, this same transformation has generally taken 2 to 5times longer. One of the major issues related to Korea's rapidly aging population relates to the health problems of the elderly. According to the 2002 National Health Survey Report, 87,6 percent of the elderly were reported to Have at least one chronic disease. In other words, almost 9out of every 10 elderly persons in Korea were suffering from a chronic illness, This, clearly, places a significant economic burden on Korean society in the form of increased health care Costs. This paper examines the increasing prevalence of chronic diseases within Korea's rapidlv aging population and offers some possible short- and long-term solutions to cope with the increased burden being placed on Korea's health care system. These include the basic services at local health centers for the elderly and the national life-long health promotion programs that aim to correct unhealthy behavior, such as smoking, the excessive use of alcohol, and poor eating and exercise habits.

• 복식
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• 제60권10호
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• pp.100-117
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• 2010

This thesis has three main purposes: first, will show kinds, patterns, types, expression techniques of modern korean women's trimmings by every ten years. Second, it is to trace the effect of each decade on women's Hanbok trimming designs. Third, it will point out factors. that caused the change of modern Korean women's Hanbok trimming design patterns. Henceforth, there comes the result of the research and analysis. Trimming design patterns of modern women's Hanbok can be divided into three categories temporally: The first period of from 1910s to the first half of 1950s saw that design patterns did not changed much from traditional ones. It is because there were many catastrophes such as the invasion of Japanese Empire and Korean War. The compound design had expressed people's wish to earn many sons and babies at the end of Joseon Dynasty. But it turned to wishing luck for individuals affected by individualism and liberalism imported from the west. Realistic designs are the most frequently used and the least lines made up patterns. The second period of from the latter half of 1950s to 1970s saw that women wore their Jeogories and Chimas as suits. Screen printing technique enabled for various design patterns to come out. And the development of textile industry introduced various expression techniques such as making lace, meshing, flocking, burn-out etc cetera. The third period of 1980s saw that Hanbok became a kind of luxurious clothe as people's living standard rose. Hanbok again came into the spotlight, Hanbok designers came forward and their individualities are respected. The characteristic expression technique of grey-dying and geometric design patterns are more frequently used for the simple and modern beauty.

• Journal of Biosystems Engineering
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• 제25권4호
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• pp.279-286
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• 2000

Most of the domestic cold storage rooms are inefficient for agricultural products because of temperature gradients inside the storage rooms. Temperature gradients are developed mainly by improper airflow pattern inside the storage room, which is a main cause of the spoilage of the agricultural products. There proper airflow pattern is essential to minimize these temperature gradients and the spoilage. The performance and characteristics of a cold storage room were determined as a function of airflow pattern and temperature distribution in forced circulation cold storage room. A commercial CFD(computational fluid dynamics) code was used to simulate 3-D airflow in the cold storage room. Solving the flow equations for the storage room, a standard k-$\varepsilon$ turbulent model was implemented to calculate steady state turbulent velocity distribution. The CFD prediction results were compared with temperature measurements inside the cold storage room. In case of pallet storage, Temperature gradients inside pallet storage was reduced because the contact area of cold air expanded through an alley of airflow in storage. But is case of bulk storage, the last temperature of storage considerably rose more than the initial temperature of storage. The reason was that bulk storage didn't include any alley of airflow in storage.

• 한국동물위생학회지
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• 제35권4호
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• pp.269-273
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• 2012

A confirmatory serological test, the standard tube agglutination test (STAT) is evaluated for the diagnostic efficiency in brucellosis Korea. A total of 345 bovine samples were collected from regional veterinary branch under national brucellosis monitoring program from January 2010 to June 2012 in Korea. These samples were diagnosed as suspected serum and brucellosis positive by the Rose Bengal test (RBT) and the STAT, respectively. The STAT was compared and evaluated with three serological test such as the indirect-enzyme linked immunosorbent assay (I-ELISA), competitive-enzyme linked immunosorbent assay (C-ELISA) and fluorescence polarisation assay (FPA) prescribed for international trade by OIE. Among the 345 bovine serum samples, 302 (87.5%) were diagnosed as positive in the STAT, while 215 (62.3%), 223 (64.6%) and 194 (56.2%) serum samples were diagnosed as positive for brucellosis in the I-ELISA, C-ELISA and FPA, respectively. The STAT showed quite high positive results as compared with three prescribed tests of OIE. FPA, I-ELISA and C-ELISA have shown 60.6%, 64.9% and 67.2% correlation, respectively as compared to the STAT. However correlations of three prescribed tests ranged high 84.1~97.7%. Especially, correlation between I-ELISA and C-ELISA is quite high, 97.7%. These results suggest that the STAT has shown many false-positive reactions. Therefore, additional serological test, such as ELISAs and FPA, would be necessary to adopt as a confirmatory test in the national surveillance program of bovine brucellosis in Korea.

• 한국방사선학회논문지
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• 제15권2호
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• pp.211-217
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• 2021

본 연구의 목적은 K 대학병원에 근무하는 방사선사들의 COVID-19 에 대한 일반적 특성, 감염 관련 특성, 지식 및 행위와 상관성이 있는 요인, 감염 예방 행위에 영향을 미치는 인자를 파악 하는 데 있다. 기본 연구는 서술적 조사연구로 2020년 3월 25일부터 6월 30일까지 K 대학병원에 근무하는 방사선사 50명을 대상으로 자료를 수집하였다. 대상자의 설문 조사로 일반적 및 감염 관련 특성은 빈도, 백분율을 산출하였고, Pearson's 상관계수 분석하였으며, 감염 예방 행위에 영향을 미치는 요인은 다중 회귀분석을 이용하였다. 통계 검정의 유의 수준은 0.05로 설정하여 P-값이 0.05 미만이면 유의하다고 해석하였다. 연구결과에서 나타난 COVID-19 감염 예방 행위 영향을 미치는 인자는 코로나 지식이 1점 오를 때 감염 예방 행위가 1.7점 올랐고, 전파경로 인식 수준이 1점 올랐을 때 감염 예방 행위가 11.3점 올랐으며, 불안정도 1점 올랐을 때 감염 예방 행위가 4.2 점 올랐다. 표준 회귀계수를 보면 예방하는데 지식, 전파경로 인식, 불안 중 가장 큰 영향을 주는 인자는 코로나의 전파경로 인식이었다. 감염 예방 행위의 영향요인으로는 코로나 지식, 전파 경로 인식과 불안이 감염 예방 가능성으로 나타나 코로나 유행 시 이 정보를 잘 이용하기를 기대한다.

• 한국항만경제학회지
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• 제34권4호
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• pp.1-16
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• 2018

대산항 수출은 석유제품, 석유화학중간원료, 기초유분, 합성수지 4개 품목이 92%를 차지하고 있다. 그런데 이와 같은 대산항의 주요 품목의 수출실적은 경쟁항만에 따라서 다르게 나타나고 있다. 본고는 이러한 현상을 규명할 수 있는 공간변이할당모형의 새로운 버전을 개발하여 대산항의 수출 변동요인을 밝힌다. 먼저 표준변이할당분석을 통해 석유제품과 기초유분에서는 지역-국가 전부문 변이효과와 지역산업구조효과가, 석유화학중간원료와 합성수지에서는 지역-국가 전부문 변이효과가 주요 수출변동요인임을 밝힌다. 석유제품을 포함한 4개 품목 모두 지역-국가 전부문 변이효과가 양의 부호를 가져 대산항의 수출이 다른 항만들에 비해 원활함을 보인다. 또한 석유제품과 기초유분의 지역산업구조효과가 양의 부호로 빠르게 수출이 증가하는 품목인데 비해, 석유화학중간원료와 합성수지는 음의 부호로 수출활동이 활발하지 못함을 보인다. 공간변이할당분석에서 대산항은 석유제품에서 경쟁력이 약한 울산항은 물론 경쟁력이 강한 온산항과 인천항에 대해서도 경쟁우위를 가지나, 경쟁력이 높은 여수항에 대해서는 열위상태에 있다. 기초유분에 있어서는 높은 경쟁력을 갖는 인천항과 온산항에 대해 그리고 경쟁력이 약해진 여수항에 대해 우위를 가지나, 경쟁력을 회복한 울산항에 대해서는 경쟁력 우위를 상실하고 있다. 석유화학중간원료에 있어서 수출경쟁력이 높은 울산항, 온산항, 여수항에 대해 경쟁력을 상실한 반면에 경쟁력이 약해진 인천항에 대해 경쟁력을 회복하고 있다. 그리고 합성수지에 있어서는 경쟁력을 가진 울산항, 부산항, 인천항에 대해 경쟁력을 잃고 있으며, 경쟁력이 약한 광양항에 대해서는 경쟁력을 유지하고 있음을 밝힌다.

• 한국게임학회 논문지
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• 제9권2호
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• pp.3-12
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• 2009

본 논문에서는 정성적, 정량적 측정을 통한 게임 감성 유희(遊戱)요소 도출을 목적으로, 공포게임의 '공포'를 '유희적 공포'라 보고 로제카이와의 놀이이론에 근거하여 분석한 후, 이를 바탕으로 공포게임 내 '유희적 공포요소'를 4가지로 분류했다. 연구자는 플레이어의 반응을 통해 이를 검증하기 위해 실증적 실험을 수행하였는데, 실험은 공포게임을 플레이 하는 동안 심박계를 통해서 각 실험자들의 심박을 측정하고 이를 촬영한 비디오 데이타를 분석하여 유희적 공포요소가 심리적, 생리적인 영향을 줄 것이라 예측된 지점을 공포지점(fear point)이라 명명하였으며, 이 지점에서 측정된 심박이 평상시 평균심박 및 실험 중 평균심박과 통계적으로 유의한 차가 있는지 알아보고 설문을 통한 플레이어의 주관적 데이타를 첨부하여 심박수와의 통계적인 상관관계를 알아보았다. 연구 결과, 공포지점에서 실험자들의 심박이 평상시 심박 및 실험 중 평균심박 보다 통계적으로 유의미하게 상승됨을 증명했으며, 주관적 데이터와도 상관관계가 높음을 보여주었다. 또한 게임 내용 분석결과 본 연구에서 주장하는 유희적 공포요소가 해당 지점에서 실험자들의 심리, 생리적 반응에 주요한 역할을 하였음을 알 수 있었다. 본 연구는 게임내 유희적 요소로서의 공포요소를 이론적인 방법과 실증적인 방법을 통해 검증하고 앞으로의 게임 제작 및 기획에 도움이 되는 기준을 제시할 수 있었다.