Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Evaluation on diagnostic efficiency of the standard tube agglutination test for bovine brucellosis

소 브루셀라병 표준시험관응집반응법 진단효율 평가

  • Sung, So-Ra (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Kim, Ji-Yeon (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Her, Moon (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Lee, Kichan (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Gu, Jeong-Hui (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Kang, Sung-Il (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Lee, Hyang-Keun (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Kim, Suk-Mi (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency) ;
  • Jung, Suk-Chan (OIE Reference Laboratory for Brucellosis, Animal, Plant and Fisheries Quarantine and Inspection Agency)
  • 성소라 (농림수산검역검사본부 동식물위생연구부 세균질병과 브루셀라병 OIE 표준실험실) ;
  • 김지연 (농림수산검역검사본부 동식물위생연구부 세균질병과 브루셀라병 OIE 표준실험실) ;
  • 허문 (농림수산검역검사본부 동식물위생연구부 세균질병과 브루셀라병 OIE 표준실험실) ;
  • 이기찬 (농림수산검역검사본부 동식물위생연구부 세균질병과 브루셀라병 OIE 표준실험실) ;
  • 구정희 (농림수산검역검사본부 동식물위생연구부 세균질병과 브루셀라병 OIE 표준실험실) ;
  • 강성일 (농림수산검역검사본부 동식물위생연구부 세균질병과 브루셀라병 OIE 표준실험실) ;
  • 이향근 (농림수산검역검사본부 동식물위생연구부 세균질병과 브루셀라병 OIE 표준실험실) ;
  • 김숙미 (농림수산검역검사본부 동식물위생연구부 세균질병과 브루셀라병 OIE 표준실험실) ;
  • 정석찬 (농림수산검역검사본부 동식물위생연구부 세균질병과 브루셀라병 OIE 표준실험실)
  • Received : 2012.10.31
  • Accepted : 2012.11.30
  • Published : 2012.12.30
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Abstract

A confirmatory serological test, the standard tube agglutination test (STAT) is evaluated for the diagnostic efficiency in brucellosis Korea. A total of 345 bovine samples were collected from regional veterinary branch under national brucellosis monitoring program from January 2010 to June 2012 in Korea. These samples were diagnosed as suspected serum and brucellosis positive by the Rose Bengal test (RBT) and the STAT, respectively. The STAT was compared and evaluated with three serological test such as the indirect-enzyme linked immunosorbent assay (I-ELISA), competitive-enzyme linked immunosorbent assay (C-ELISA) and fluorescence polarisation assay (FPA) prescribed for international trade by OIE. Among the 345 bovine serum samples, 302 (87.5%) were diagnosed as positive in the STAT, while 215 (62.3%), 223 (64.6%) and 194 (56.2%) serum samples were diagnosed as positive for brucellosis in the I-ELISA, C-ELISA and FPA, respectively. The STAT showed quite high positive results as compared with three prescribed tests of OIE. FPA, I-ELISA and C-ELISA have shown 60.6%, 64.9% and 67.2% correlation, respectively as compared to the STAT. However correlations of three prescribed tests ranged high 84.1~97.7%. Especially, correlation between I-ELISA and C-ELISA is quite high, 97.7%. These results suggest that the STAT has shown many false-positive reactions. Therefore, additional serological test, such as ELISAs and FPA, would be necessary to adopt as a confirmatory test in the national surveillance program of bovine brucellosis in Korea.

Keywords

References

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