• Journal of Environmental Health Sciences
• /
• v.45 no.5
• /
• pp.511-519
• /
• 2019

Objectives: The standard method for the enumeration of environmental Legionella is culturing, which has several disadvantages, including long incubation and poor sensitivity. The purpose of this study is to demonstrate the usefulness of real-time PCR and to improve the standard method. Methods: In 200 environmental water samples, a real-time PCR and culture were conducted to detect and quantify Legionella. Using with the results of the survey, we compared the real-time PCR with the culture. Results: Each real-time PCR assay had 100% specificity and excellent sensitivity (5 GU/reaction). In the culture, 36 samples were positive and 164 samples were negative. Based on the results of the culture, real-time PCR showed a high negative predictive value of 99%, 35 samples were true positive, 105 samples were true negative, 59 samples were false positive and one sample was a false negative. Quantitative analysis of the two methods indicated a weak linear correlation ($r^2=0.29$, $r^2=0.61$, respectively). Conclusions: Although it is difficult to directly apply quantitative analysis results of real-time PCR in the enumeration of environmental Legionella, it can be used as a complementary means of culturing to rapidly screen negative samples and to improve the accuracy of diagnosis.

• Asian Pacific Journal of Cancer Prevention
• /
• v.17 no.12
• /
• pp.5265-5272
• /
• 2016

Objective: The present study was conducted to evaluate invasive and noninvasive diagnostic methods for detection of Helicobacter pylori (H. pylori) in patients admitted with dyspeptic complaints and to compare sensitivities and specificities. Method: Sets of four gastric biopsy specimens were obtained from a total of 126 patients included in the study. The presence of H. pylori was determined by invasive tests including culture, rapid urease test, polymerase chain reaction (PCR) and histopathology. Among noninvasive tests, urea breath test, serological tests and enzyme-linked immunosorbent assay (ELISA) were performed. Results: H. pylori was isolated in 79 (62.7%) gastric biopsy cultures, whereas positivity was concluded for 105 (83.3%) patients by rapid urease test, for 106 (84.1%) by PCR, for 110 (87.3%) by histopathology, for 119 (94.4%) by urea breath test, and for 107 (84.9%) by ELISA. In the present study, the culture findings and histopathological examination findings were accepted as gold standard. According to the gold standard, urea breath test had the highest sensitivity (96.5%) and the lowest specificity (30%), whereas culture and histopathology had the highest specificities (100%). Conclusion: The use of PCR invasively with gastric biopsy samples yielded parallel results with the gold standard. PCR can be recommended for routine use in the diagnosis of H. pylori.

• The Research Journal of the Costume Culture
• /
• v.14 no.3 s.62
• /
• pp.483-496
• /
• 2006

The purpose of this study was to develop a pragmatic fashion internship program as a following study of 'An analysis of the consciousness of the professors and college students for the fashion industry internship'. The methods of the quantitative analysis and some case studies were used for this study. The results of this study were as follows : Internship program has 6 steps ; ready, introduction, selection, operation, reputation, management. It will help professors and college students to execute the program systematically. The contents of the fashion internship program model for professors were as follows: 8 recruiting methods to find internship companies, the contents of the internship agreement, 6 types of internship, the contents of the recommendation letter, advanced education contents for a fashion internship, e-mentoring program model, a curriculum of the cultural study and the major course, evaluation standard elements of the intern students, the methods of evaluation etc.

• The Journal of the Convergence on Culture Technology
• /
• v.7 no.4
• /
• pp.503-510
• /
• 2021

Purpose: This study was attempted to identify knowledge, performance of standard precautions, and nursing professionalism and to investigate the influence on nursing professionalism in nursing students experienced clinical practice. Methods: The subjects are 205 nursing students in six university nursing departments in two regions. The data were collected from September 7th to September 17th, 2021 using the online survey. Results: The total score for knowledge of standard precautions was 24.69. The average for performance of standard precautions was 3.70 on a scale of 4 and nursing professionalism was an average of 4.12 on a scale of 5. Factors affecting nursing professionalism included gender(β=-.21, p=.002), knowledge of standard precautions(β=.13, p=.044), and performance of standard precautions(β=.27, p=<.001). The explanatory power of the model was 13.1%. Conclusions: Results of this study suggest that it is necessary to develop and apply an clinical educational program that can promote knowledge and performance of standard precautions to enhance the positive nursing professionalism of nursing students.

• Archives of Pharmacal Research
• /
• v.21 no.3
• /
• pp.253-259
• /
• 1998

In this study, we developed immunoassay methods for the more convenient and effective detection of rat tracheal mucin and the results were compared with those of [$3^H$]glucosamine based gel-filtratioh method. A monoclonal anti-rat tracheal mucin antibody, mAbRT03, which specifically recognizes rat tracheal mucins, was used throughout in this study. To induce mucin secretion, varying concentrations of ATP (0-2 mM) were applied to the primary rat tracheal surface epithelial (RTSE) cell culture which had been metabolically radiolabeled with [$3^H$]glucosamine and the secretion of mucin was analyzed both by the immunoassay and the gel-filtration chromatography methods. For the immunoassay, the following two procedures were employed. 1) Simple ELISA; the culture spent media were directly coated onto the assay plate and the immunoreactivity with mAbRT03 was assessed from the standard curve generated with the purified rat mucin. 2) Inhibition ELISA; A known amount of the purified rat mucin was coated onto the assay plate and then ATP-stimulated culture spent media were added to inhibit the immunorelitivity with mAbRT03. The contents of mucin in the sample were calculated from the standard inhibition curve which was generated with the purified rat mucin. The assay results obtained from the immunoassays were identical with those from the gel-filtration methods. The present result indicates that ELISA can be substituted for the laborious, time-consuming gel-filtration assay in studying the regulation of airway mucin release from cultured airway epithelial cells.

• Korean Institute of Interior Design Journal
• /
• no.28
• /
• pp.11-16
• /
• 2001

All vague and constructions of contemporary cultural environment have been reorganized and the relationship between people and culture can't follow development speed of rapid material civilization development and being informationalized. Because of the circumstances, physical environment is changing into other cultural environment, existence of human being is at a crisis. Physical value precedes mental value and rapid changing causes culture lag. Modernism design of 20th century suggested standard, simplicity and mass production methods, but nowaday, as human being complexity is increasing, information-media are needed to satisfy that. Namely usual design thoughts have to be changed because our life is surrounded by many kinds of powerful media sings and various mixed cultural circumstances. People predict that the characteristics of new ages are 'Immateriality', 'Sensoriality', 'multimedia'. Amongst them, medial spatial characteristics will be eventually remarkable in commercial space which is coincided with economic logic and multilateral culture. This study is a focus on commercial space which emphasis contemporary medial characteristics and observes medial meaning and various technique through some experimental works in Korea. Also this study's purpose is to examine design directions which correspond with contemporary Korea culture.

• Clinical and Experimental Pediatrics
• /
• v.58 no.5
• /
• pp.183-189
• /
• 2015

Purpose: Catheter urine (CATH-U) and suprapubic aspiration (SPA) are reliable urine collection methods for confirming urinary tract infections (UTI) in infants. However, noninvasive and easily accessible collecting bag urine (CBU) is widely used, despite its high contamination rate. This study investigated the validity of CBU cultures for diagnosing UTIs, using CATH-U culture results as the gold standard. Methods: We retrospectively analyzed 210 infants, 2- to 24-month-old, who presented to a tertiary care hospital's pediatrics department between September 2008 and August 2013. We reviewed the results of CBU and CATH-U cultures from the same infants. Results: CBU results, relative to CATH-U culture results (${\geq}10^4$ colony-forming units [CFU]/mL) were widely variable, ranging from no growth to ${\geq}10^5CFU/mL$. A CBU cutoff value of ${\geq}10^5CFU/mL$ resulted in false-positive and false-negative rates of 18% and 24%, respectively. The probability of a UTI increased when the CBU bacterial count was ${\geq}10^5/mL$ for all infants, both uncircumcised male infants and female infants (likelihood ratios [LRs], 4.16, 4.11, and 4.11, respectively). UTIs could not be excluded for female infants with a CBU bacterial density of $10^4-10^5$ (LR, 1.40). The LRs for predicting UTIs based on a positive dipstick test and a positive urinalysis were 4.19 and 3.11, respectively. Conclusion: The validity of obtaining urine sample from a sterile bag remains questionable. Inconclusive culture results from CBU should be confirmed with a more reliable method.

• Journal of Genetic Medicine
• /
• v.11 no.2
• /
• pp.43-48
• /
• 2014

Chorionic villus sampling has gained importance as a tool for early cytogenetic diagnosis with a shift toward first trimester screening. First trimester screening using nuchal translucency and biomarkers is effective for screening. Chorionic villus sampling generally is performed at 10-12 weeks by either the transcervical or transabdominal approach. There are two methods of analysis; the direct method and the culture method. While the direct method may prevent maternal cell contamination, the culture method may be more representative of the true fetal karyotype. There is a concern for mosaicism which occurs in approximately 1% of cases, and mosaic results require genetic counseling and follow-up amniocentesis or fetal blood sampling. In terms of complications, procedure-related pregnancy loss rates may be the same as those for amniocentesis when undertaken in experienced centers. When the procedure is performed after 9 weeks gestation, the risk of limb reduction is not greater than the risk in the general population. At present, chorionic villus sampling is the gold standard method for early fetal karyotyping; however, we anticipate that improvements in noninvasive prenatal testing methods, such as cell free fetal DNA testing, will reduce the need for invasive procedures in the near future.

• The Korean Journal of Health Service Management
• /
• v.7 no.4
• /
• pp.81-92
• /
• 2013

This research was conducted to analyze the perception of the dental hygienists who work in ordinary dental clinics regading patient safety culture and to analyze the related elements. As for the research subjects and methods, 233 dental hygienists who work at the dental clinics located in Daegu, Gyeongbuk and Jeonnam were targeted from February 6, 2012 to March 5, 2012 to administer survey by utilizing patient safety culture recognition tool. Collected data was subjected to the SPSS 18.0 program to calculate average and standard deviation, and to carry out t-test, ANOVA and multiple regression analysis. Dental hygienists' level of perception towards patient safety culture was mid level, and it was demonstrated that there is a correlation with age, work experience at department, title and type of department that affect level of perception towards patient safety culture. In conclusion, Since this research targeted only a limited number of dental hygienists, it is necessary to be cautious about over-interpreting the results. Above anything, it is necessary to carry out repetitive research by targeting medical professionals of diverse jobs.

• Proceedings of the Korea Contents Association Conference
• /
• 2007.11a
• /
• pp.724-726
• /
• 2007

Many enterprises make use of 'mecenat action' for positive image ensuring to company and harmony within organization members. Also, at the same time the research has continued estimating of efficiency about that area. but that estimations get accomplished inclusive and abstractive, and then estimation of significant effect isn't sufficient. In this study we consider that many enterprises practice culture and art education program, and related that analyze the estimation of effect in 'mecenat action'. Stand on this analysis we attempts to extract factor, having representation character with making standard for estimation of education effect. That is a implication that relation education programs are prepared many enterprises, In future study, we will need to make standard and select methods about effect estimation, add measurable index that reliability can increase.