Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Evaluation of Invasive and Noninvasive Methods for the Diagnosis of Helicobacter Pylori Infection

  • Cosgun, Yasemin (Public Health Institute of Turkey Microbiology Reference Laboratories Department, Ankara Training and Research Hospital) ;
  • Yildirim, Abdullah (Gaziantep University, Faculty of Medicine) ;
  • Yucel, Mihriban (Department of Medical Microbiology, Ankara Training and Research Hospital) ;
  • Karakoc, Ayse Esra (Department of Medical Microbiology, Ankara Training and Research Hospital) ;
  • Koca, Gokhan (Department of Nuclear Medicine, Ankara Training and Research Hospital) ;
  • Gonultas, Alpaslan (Department of Medical Pathology, Ankara Training and Research Hospital) ;
  • Gursoy, Gul (Departement of Internal Medicine, Ankara Education and Research Hospital) ;
  • Ustun, Huseyin (Department of Medical Pathology, Ankara Training and Research Hospital) ;
  • Korkmaz, Meliha (Department of Nuclear Medicine, Ankara Training and Research Hospital)
  • Published : 2016.12.01
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Abstract

Objective: The present study was conducted to evaluate invasive and noninvasive diagnostic methods for detection of Helicobacter pylori (H. pylori) in patients admitted with dyspeptic complaints and to compare sensitivities and specificities. Method: Sets of four gastric biopsy specimens were obtained from a total of 126 patients included in the study. The presence of H. pylori was determined by invasive tests including culture, rapid urease test, polymerase chain reaction (PCR) and histopathology. Among noninvasive tests, urea breath test, serological tests and enzyme-linked immunosorbent assay (ELISA) were performed. Results: H. pylori was isolated in 79 (62.7%) gastric biopsy cultures, whereas positivity was concluded for 105 (83.3%) patients by rapid urease test, for 106 (84.1%) by PCR, for 110 (87.3%) by histopathology, for 119 (94.4%) by urea breath test, and for 107 (84.9%) by ELISA. In the present study, the culture findings and histopathological examination findings were accepted as gold standard. According to the gold standard, urea breath test had the highest sensitivity (96.5%) and the lowest specificity (30%), whereas culture and histopathology had the highest specificities (100%). Conclusion: The use of PCR invasively with gastric biopsy samples yielded parallel results with the gold standard. PCR can be recommended for routine use in the diagnosis of H. pylori.

Keywords

References

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