• 한국주조공학회지
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• 제40권4호
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• pp.118-127
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• 2020

Microstructural evolution during a heat treatment and high-temperature tensile properties have been investigated in conventionally cast CM247LC. In as-cast specimens, MC carbides with high amounts of Ta, Ti, Hf, and W were found to exist in the interdendritic regions, and γ' was observed in the form of cubes and octocubes prior to decomposition into cubes. In the heat-treated condition, some portion of eutectic γ-γ' remained, and uniform cubic γ' was observed in both interdendritic regions and dendrite core. Three types of carbides with different stoichiometries and compositions were found at the grain boundaries. MC carbides with high Hf contents were observed in the vicinity of eutectic γ-γ'. The highest tensile strength value was found at 750℃, whereas the greatest ductility appeared at 649℃. The effect of the temperature on the tensile properties was closely related to the dislocation structure. With increase in the test temperature, the density of dislocations inside γ' decreased, whereas that in the γ matrix increased. Stacking faults generated in γ' at 750℃ had a strengthening effect, whereas thermally activated dislocation motion at a high temperature was considered to have the opposite effect.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.287.1-287.1
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• 2013

The Isolation of few-layered transition metal dichalcogenides has mainly been performed by mechanical and chemical exfoliation with very low yields. in particular, the two-dimensional layer of molybdenum disulfide (MoS2) has recently attracted much interest due to its direct-gap property and potential application in optoelectronics and energy harvesting. However, the synthetic approach to obtain high-quality and large-area MoS2 atomic thin layers is still rare. In this account, a controlled thermal reductionsulfurization method is used to synthesize large-MoOx thin films are first deposited on Si/SiO2 substrates, which are then sulfurized (under vacuum) at high temperatures. Samples with different thicknesses have been analyzed by Raman spectroscopy and TEM, and their photoluminescence properties have been evaluated. We demonstrated the presence of single-, bi-, and few-layered MoS2 on as-grown samples. It is well known that the electronic structure of these materials is very sensitive to the number of layer, ranging from indirect band gap semiconductor in the bulk phase to direct band gap semiconductor in monolayers. This synthetic approach is simple, scalable, and applicable to other transition metal dichalcogenides. Meanwhile, the obtained MoS2 films are transferable to arbitrary substrates, providing great opportunities to make layered composites by stacking various atomically thin layers.

• Molecules and Cells
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• 제43권9호
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• pp.784-792
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• 2020

Arginine kinase (AK), a bioenergy-related enzyme, is distributed widely in invertebrates. The role of highly conserved histidines in AKs is still unascertained. In this study, the highly conserved histidine 284 (H284) in AK of Daphnia magna (DmAK) was replaced with alanine to elucidate the role of H284. We examined the alteration of catalytic activity and structural changes of H284A in DmAK. The catalytic activity of H284A was reduced dramatically compared to that in wild type (WT). Thus the crystal structure of H284A displayed several structural changes, including the alteration of D324, a hydrogen-bonding network around H284, and the disruption of π-stacking between the imidazole group of the H284 residue and the adenine ring of ATP. These findings suggest that such alterations might affect a conformational change of the specific loop consisting of G310-V322 at the antiparallel β-sheet region. Thus, we speculated that the H284 residue might play an important role in the conformational change of the specific loop when ATP binds to the substrate-binding site of DmAK.

• 한국항공우주학회지
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• 제47권10호
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• pp.687-694
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• 2019

초경량 항공기 구조의 날개 보로 사용되는 원통형 복합재료 날개 보는 굽힘 모멘트와 전단하중을 동시에 받고 있는 구조물이다. 하지만 기존의 일반 원통형 보는 상하부의 굽힘 모멘트, 좌우부의 전단하중을 지지하는 구조적 특성을 고려하지 못하므로 비효율적일 수 있다. 따라서 섬유각 또는 복합재료를 적절히 배열하여 효율적으로 구조물을 만드는 것이 필요하다. 본 연구에서는 원통형 복합재료 보의 굽힘강도와 전단강도의 증가를 위해 보의 단면을 상하좌우로 분할하여 적층순서를 달리함으로써 효율적인 하중지지가 가능하게 하였다. 상용 프로그램 MSC/NASTRAN을 이용한 구조해석을 통해 원호 분할각과 섬유각에 따른 수직변위, 수직변형률, 전단변형률 계산하였다. 계산 결과에 따르면 새롭게 제안된 원통형 보의 분할각과 섬유 방향각을 선택하여 구조 강도를 증가시킬 수 있음을 제시하였다.

• 한국염색가공학회지
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• 제30권4호
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• pp.313-320
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• 2018

As carbon fiber reinforced composites(CFRP) are widely used in aerospace, automobile, marine, and sports goods applications, they have been studied extensively by various researchers. However, CFRP have been pointed out because of machining problems such as delamination and burr phenomenons. Especially, hole machining process, drilling, has non-smooth features on inlet and outlet surfaces of drilled hole. This kind of machining problem can be controlled to some extent by using high modulus pitch-CF, which has considerable effects on fracture behavior of composite compared with only PAN CF composite. Therefore, PAN and pitch hybridized CF composites were prepared having high strength and modulus. The results demonstrate that the hybrid CFRP specimens with pitch CF offer the good potential to enhance modulus as well as strength properties. Dynamic mechanical, flexural, and impact properties were measured and analyzed. Morphological surface of the composites were also observed by IFS-28, canon after hole machining.

• 한국정보기술학회논문지
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• 제16권11호
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• pp.85-95
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• 2018

스마트폰의 보급이 확산되고 대중화됨에 따라 대부분의 사람들은 사진을 촬영하기 위해 모바일 카메라를 애용하고 있다. 하지만 저조도 환경에서 사진을 촬영할 때 광량이 부족한 이유로 원치 않는 잡음이 발생할 수 있다. 이런 잡음을 제거하기 위해, 최근 심층 합성곱 신경망에 기반한 잡음 제거 기법이 제안되었다. 이 기법은 성능 측면에서 큰 진전을 보였을지라도 여전히 텍스처 및 에지 표현 능력이 부족하다. 따라서 본 논문에서는 영상의 구조를 향상시키기 위해 에지의 방향 정보를 나타내는 호그 영상을 활용하고자 한다. 그리고 잡음 영상과 호그 영상을 스택으로 쌓은 후, 입력 텐서를 형성하여 심층 합성곱 신경망을 학습시키는 기법을 제안하고자 한다. 실험 결과를 통해, 제안한 기법은 기존의 기법보다 정량적인 화질 평가에서 더 우수한 결과를 얻을 수 있었으며 시각적인 측면에서도 텍스처 및 에지의 향상을 달성할 수 있었다.

• 대한토목학회논문집
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• 제44권2호
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• pp.141-147
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• 2024

본 연구에서는 마이크로 CT 이미지를 활용하여 3D 프린팅 콘크리트의 공극분포 특성에 따른 인장파괴 강도를 확인하였다. 3D 프린팅 기법으로 출력된 콘크리트 구조물은 일반적인 시편과는 다르게 적층방향 및 필라멘트 접촉면의 존재에 따라 공극의 방향성을 갖는다. 이에 따라 3D 프린팅 콘크리트 시편의 공극분포를 확률론적 방법으로 분석하고, 유한요소기법을 통해 방향별 인장강도를 분석하였다. 3D 프린팅된 시편 내부의 공극이 방향성을 갖는 것을 확인하였고, 출력에 의한 미세구조 특성-강도의 영향성을 평가하였다. 본 연구는 마이크로 CT 이미지 기반의 공극 분포 특성을 분석하고 시뮬레이션을 활용한 기계적 물성 평가를 수행하여 보다 향상된 성능의 적층 구조물 설계 및 재료 개발에 활용하고자 한다.

• Journal of Microbiology and Biotechnology
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• 제34권8호
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• pp.1660-1670
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• 2024

The aim of this study was to modify phytase YiAPPA via protein surficial residue mutation to obtain phytase mutants with improved thermostability and activity, enhancing its application potential in the food industry. First, homology modeling of YiAPPA was performed. By adopting the strategy of protein surficial residue mutation, the lysine (Lys) and glycine (Gly) residues on the protein surface were selected for site-directed mutagenesis to construct single-site mutants. Thermostability screening was performed to obtain mutants (K189R and K216R) with significantly elevated thermostability. The combined mutant K189R/K216R was constructed via beneficial mutation site stacking and characterized. Compared with those of YiAPPA, the half-life of K189R/K216R at 80℃ was extended from 14.81 min to 23.35 min, half-inactivation temperature (T₅₀³⁰) was increased from 55.12℃ to 62.44℃, and T_m value was increased from 48.36℃ to 53.18℃. Meanwhile, the specific activity of K189R/K216R at 37℃ and pH 4.5 increased from 3960.81 to 4469.13 U/mg. Molecular structure modeling analysis and molecular dynamics simulation showed that new hydrogen bonds were introduced into K189R/K216R, improving the stability of certain structural units of the phytase and its thermostability. The enhanced activity was primarily attributed to reduced enzyme-substrate binding energy and shorter nucleophilic attack distance between the catalytic residue His28 and the phytate substrate. Additionally, the K189R/K216R mutant increased the hydrolysis efficiency of phytate in food ingredients by 1.73-2.36 times. This study established an effective method for the molecular modification of phytase thermostability and activity, providing the food industry with an efficient phytase for hydrolyzing phytate in food ingredients.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2011년도 춘계학술발표대회
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• pp.8.1-8.1
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• 2011

Nanocrystalline titanium dioxide ($TiO_2$) materials have been widely used as an electron collector in DSSC. This is required to have an extremely high porosity and surface area such that the dye can be sufficiently adsorbed and be electronically interconnected, resulting in the generation of a high photocurrent within cells. In particular, their geometrical structures and crystalline phase have been extensively investigated as important issues in improving its photovoltaic efficiency. In this study, we present a new strategy to fabricate a photoelectrode having a periodic structured $TiO_2$ film templated from 1D or 3D polystyrene (PS) microspheres array. Monodisperse PS spheres of various radiuses were used for colloidal array on FTO glasses and two types of photoelectrode structures with different $TiO_2$ materials were investigated respectively. One is the igloo-shaped electrode prepared by $TiO_2$ deposition by RF-sputtering onto 2D microsphere-templated substrates. At the interface between the film and substrate, there are voids formed by the decomposition of PS microspheres during the calcination step. These holes might be expected to play the predominant roles as scattering spherical voids to promote a light harvesting effect, a spacious structure for electrolytes with higher viscosity and effective paths for electron transfer. Additionally the nanocrystalline $TiO_2$ phase prepared by the RF-sputtering method was previously reported to improve the electron drift mobility within $TiO_2$ electrodes. This yields solar cells with a cell efficiency of 2.45% or more at AM 1.5 illumination, which is a very remarkable result, considering its $TiO_2$ electrode thickness (<2 ${\mu}m$). This study can be expanded to obtain higher cell efficiency by higher dye loading through the increase of surface area or multi-layered stacking. The other is the inverse opal photonic crystal electrode prepared by titania particles infusion within 3D colloidal arrays. To obtain the enlargement of ordered area and high quality of crystallinity, the synthesis of titania particles coated with a organic thin layer were applied instead of sol-gel process using the $TiO_2$ precursors. They were dispersed so well in most solvents without aggregates and infused successfully within colloidal array structures. This ordered mesoporous structure provides the large surface area leading to the enough adsorption of dye molecules and have an light harvesting effect due to the photonic band gap properties (back-and-forth reflection effects within structures). A major advantage of this colloidal array template method is that the pore size and its distribution within $TiO_2$ photoelectrodes are determined by those of latex beads, which can be controlled easily. These materials may have promising potentials for future applications of membrane, sensor and so on as well as solar cells.

• 한국진공학회지
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• 제19권3호
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• pp.217-223
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• 2010

유기금속화학기상증착법으로 적층 InAs/$In_{0.1}Ga_{0.9}As$ DWELL (dot-in-a-well) 구조를 성장하여 n-i-n 구조의 적외선 수광소자를 제작하였으며, PL (photoluminescence) 발광 특성 및 암전류 특성을 분석하였다. 동일한 조건으로 양자점을 적층하였을 때 크기 및 밀도의 변화에 의한 이중 PL peak을 관찰하였으며, TMIn의 유량을 조절함으로써 단일 peak을 갖는 균일한 크기의 양자점 적층 구조를 성장할 수 있었다. 적외선 수광소자 구조를 성장함에 있어서, 상부의 n-형 GaAs의 성장 온도가 600도 이상인 경우 PL 발광 세기가 급격히 감소하였고 이에 따른 암전류의 증가를 관찰하였다. 0.5 V 인가 전압에서 암전류의 온도 의존성에 대한 활성화 에너지의 크기는 성장온도가 580도인 경우 106 meV이고, 650도의 경우는 48 meV로 급격이 낮아졌다. 이는 고온의 성장 온도에 의한 InAs 양자점과 $In_{0.1}Ga_{0.9}As$ 양자우물구조 계면에서의 열적 상호 확산에 의하여 비발광 천이가 증가되었기 때문이다.