• 한국환경농학회지
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• 제25권4호
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• pp.371-381
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• 2006

New proteomic techniques have been pioneered extensively in recent years, enabling the high-throughput and systematic analyses of cellular proteins in combination with bioinformatic tools. Furthermore, the development of such novel proteomic techniques facilitates the elucidation of the functions of proteins under stress or disease conditions, resulting in the discovery of biomarkers for responses to environmental stimuli. The ultimate objective of proteomics is targeted toward the entire proteome of life, subcellular localization biochemical activities, and the regulation thereof. Comprehensive analysis strategies of proteomics can be classified into three categories: (i) protein separation via 2-dimensional gel electrophoresis (2-DE) or liquid chromatography (LC), (ii) protein identification via either Edman sequencing or mass spectrometry (MS), and (iii) proteome quantitation. Currently, MS-based proteomics techniques have shifted from qualitative proteome analysis via 2-DE or 2D-LC coupled with off-line matrix assisted laser desorption ionization (MALDI) and on-line electrospray ionization (ESI) MS, respectively, toward quantitative proteome analysis. In vitro quantitative proteomic techniques include differential gel electrophoresis with fluorescence dyes. protein-labeling tagging with isotope-coded affinity tags, and peptide-labeling tagging with isobaric tags for relative and absolute quantitation. In addition, stable isotope-labeled amino acids can be in vivo labeled into live culture cells via metabolic incorporation. MS-based proteomics techniques extend to the detection of the phosphopeptide mapping of biologically crucial proteins, which ale associated with post-translational modification. These complementary proteomic techniques contribute to our current understanding of the manner in which life responds to differing environment.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제3권4호
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• pp.399-422
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• 2009

Although current wireless mesh network (WMN) applications experience a perceptually uninterrupted hand-off, their throughput after the hand-off event may be significantly degraded due to the low available bandwidth of the mobile client's new master. In this paper, we propose a novel mobility management scheme for 802.11-based WMNs that enables both seamless hand-off for transparent communications, and bandwidth awareness for stable application performance after the hand-off process. To facilitate this, we (i) present a new buffer moment in support of the fast Layer-2 hand-off mechanism to cut the packet loss incurred in the hand-off process to zero and (ii) design a dynamic admission control to grant joining accepts to mesh clients. We evaluate the benefits and drawbacks of the proposal for both UDP and TCP traffic, as well as the fairness of the proposal. Our results show that the new scheme can not only minimize hand-off latency, but also maintain the current application rates of roaming users by choosing an appropriate new master for joining.

• Journal of Information Technology Applications and Management
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• 제10권3호
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• pp.1-22
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• 2003

P2P systems have been studied by many researchers in universities and commercial firms in recent years. In this study. we design and implement a system that makes UP for shortcomings of currently available P2P systems. Gnutella and Napster. The study also includes an efficiency analysis scheme conducted through a series of experimental data. The data sharing system of the study demonstrated duality of roles(client, service) of peers. But, their roles were separated from the existing client-server systems. Also, the study implements mechanism that shows the redundancy of data to communicate efficiently among peers for transferring data. The results of performance measure of the system shows that the amount of information shared by peers increases as the amount of peers increases but with no significant increase in response time. This constant response time is far more stable and faster than current file sharing systems. such as Gnutella and Napster. Business applications such as knowledge management, enterprise information portal management and transfer of data are done by use of supercomputers. They need to extend their systems to equip with more capacity and throughput as the number of clients increases. Moreover, they will face with more complicated problems if integration with new systems exists. If this JDSS is introduced to these business applications. it would easily augment scalability of the system with high performance at less expense.

• Mass Spectrometry Letters
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• 제2권3호
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• pp.69-72
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• 2011

Defective synthesis of the steroid hormones by the adrenal cortex has profound effects on human development and homeostasis. Due to the time-consuming chromatography procedure combined with mass spectrometry, the matrix-assisted laser desorption ionization method coupled to the linear ion-trap tandem mass spectrometry (MALDI-LTQ-MS/MS) was developed for quantitative analysis of 10 adrenal steroids in human serum. Although MALDI-MS can be introduced for its applicability as a high-throughput screening method, it has a limitation on reproducibility within and between samples, which renders poor reproducibility for quantification. For quantitative MALDI-MS/MS analysis, the stable-isotope labeled internal standards were used and the conditions of crystallization were tested. The precision and accuracy were 3.1~35.5% and 83.8~138.5%, respectively, when a mixture of 10 mg/mL ${\alpha}$-cyano-4-hydroxycinnamic acid in 0.2% TFA of 70% acetonitrile was used as the MALDI matrix. The limit of quantification ranged from 5 to 340 ng/mL, and the linearity as a correlation coefficient was higher than 0.988 for all analytes in the calibration range. Clinical applications include quantitative analyses of patients with congenital adrenal hyperplasia. The devised MALDI-MS/MS technique could be successfully applied to diagnosis of clinical samples.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제11권4호
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• pp.1866-1888
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• 2017

Efficient energy consumption in WSN is one of the key design issues for improving network stability period. In this paper, we propose a new Heterogeneity-aware Energy-efficient Clustering (HEC) technique which considers two types of heterogeneity - network lifetime and of sensor nodes. Selection of cluster head nodes is done based on the three network lifetime phases: only advanced nodes are allowed to become cluster heads in the initial phase; in the second active phase all nodes are allowed to participate in cluster head selection process with equal probability, and in the last dying out phase, clustering is relaxed by allowing direct transmission. Simulation-based performance analysis of the proposed technique as compared to other relevant techniques shows that HEC achieves longer stable region, improved throughput, and better energy dissipation owing to judicious consumption of additional energy of advanced nodes. On an average, the improvement observed for stability period over LEACH, SEP, FAIR and HEC- with SEP protocols is around 65%, 30%, 15% and 17% respectively. Further, the scalability of proposed technique is tested by varying the field size and number of sensing nodes. The results obtained are found to be quite optimistic. The impact of energy heterogeneity has also been assessed and it is found to improve the stability period though only upto a certain extent.

• 한국기계가공학회지
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• 제19권8호
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• pp.110-116
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• 2020

Aircraft hydraulic oil reservoirs made with aluminum 7075 have an anodized coating to enable airtightness and corrosion resistance. To maintain a stable oil pressure, the internal surface roughness of the reservoir should be less than approximately 0.2 ㎛. To this end, precision polishing must be performed. However, ensuring the processing quality is challenging, as most polishing operations are performed manually, owing to which, the inner surface roughness is not uniform, and the product quality is irregular. Therefore, we developed a special superfinishing machine to realize the efficient inner polishing of an aircraft hydraulic oil reservoir, by using an abrasive film to improve the process throughput and uniformity. In the experiment involving the superfinishing of an anodized aluminum 7075 cylinder specimen by using the proposed machine, a higher surface roughness than that achieved in the repetitive manual polishing process could be realized.

• Biomolecules & Therapeutics
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• 제26권3호
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• pp.290-297
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• 2018

We aimed to understand the molecular changes in host cells that accompany infection by the seasonal influenza A H1N1 virus because the initial response rapidly changes owing to the fact that the virus has a robust initial propagation phase. Human epithelial alveolar A549 cells were infected and total RNA was extracted at 30 min, 1 h, 2 h, 4 h, 8 h, 24 h, and 48 h post infection (h.p.i.). The differentially expressed host genes were clustered into two distinct sets of genes as the infection progressed over time. The patterns of expression were significantly different at the early stages of infection. One of the responses showed roles similar to those associated with the enrichment gene sets to known 'gp120 pathway in HIV.' This gene set contains genes known to play roles in preventing the progress of apoptosis, which infected cells undergo as a response to viral infection. The other gene set showed enrichment of 'Drug Metabolism Enzymes (DMEs).' The identification of two distinct gene sets indicates that the virus regulates the cell's mechanisms to create a favorable environment for its stable replication and protection of gene metabolites within 8 h.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제14권5호
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• pp.1984-2002
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• 2020

With the appearance of wireless spectrum crisis in traditional cellular network, device-to-device (D2D) communication has been regarded as a promising solution to ease heavy traffic burden by enabling precise content delivery among mobile users. However, due to the channel sharing, the interference between D2D and cellular users can affect the transmission rate and narrow the throughput in the network. In this paper, we firstly present a weighted interference minimization cluster formation model involving both social attribute and physical closeness. The weighted-interference, which is evaluated under the susceptible-infected(SI) model, is utilized to gather user in social and physical proximity. Then, we address the cluster formation problem via spectrum clustering with iterative operation. Finally, we propose the stable matching theory algorithm in order to maximize rate oriented to accomplish the one-to-one resource allocation. Numerical results show that our proposed scheme acquires quite well clustering effect and increases the accumulative transmission rate compared with the other two advanced schemes.

• KSBB Journal
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• 제29권4호
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• pp.278-284
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• 2014

We present microfluidic method to rapidly analyze the effect of temperature on the change of morphologies of Antarctic bacteria (Pseudoalteromonas sp., Shewanella vesiculosa, Shewanella sp., and Cellulophaga sp.). The microfluidic device is able to generate stable temperature gradient from 7 to$40^{\circ}C$ and dramatically reduce the number of experiments, experimental cost and labor, and amount of sample. Based on this approach, we found that specific bacteria transforming morphology into filament or elongated body strongly depends on cultivation temperature. Interestingly, we found that the morphologies of Pseudoalteromonas sp., Shewanella vesiculosa, Shewanella sp., and Cellulophaga sp. are elongated at below $25^{\circ}C$, above $20^{\circ}C$, above $15^{\circ}C$ and above $35^{\circ}C$, respectively. We envision the microfluidic device is a useful approach to analyze biological events with a high throughput manner.

• 한국통신학회논문지
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• 제32권4A호
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• pp.330-335
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• 2007

본 논문에서는 멀티 홉 셀룰라 망에서 안정적인 멀티채널 랜덤 액세스 수율 보장을 위한 기지국 및 릴레이 노드 간의 랜덤 액세스 채널 할당 방안을 제안한다. 제안하는 방안에서는 채널 부하에 따라 기지국 및 릴레이 노드 간 랜덤 액세스 채널 할당 비율을 동적으로 조절할 수 있는 수학적 기준을 제시한다. 또한 랜덤 액세스 채널 할당 비율 조절만으로는 안정적 수율을 보장할 수 없는 높은 부하 상황에서는 랜덤 액세스 패킷 재전송 확률 조절 방식을 통해 안정적인 랜덤 액세스 수율을 보장하고자 한다. 시뮬레이션 결과는 제안하는 방안이 랜덤 액세스 패킷 생성률이 0.1 이상이 되는 고 부하 상황에서도 안정적인 채널 효율 및 패킷 전송 지연을 보장함을 보인다.