• Ocean Science Journal
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• 제42권4호
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• pp.223-230
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• 2007

To understand the origin and biogeochemistry of the organic matter in surface sediments of Lake Shihwa and Lake Hwaong, organic nitrogen, inorganic nitrogen, labile organic carbon, and residual organic carbon contents as well as stable isotope ratios for carbon and nitrogen were determined by KOBr-KOH treatment. Ratios of organic carbon to organic nitrogen $(C_{org}/N_{org})$ (mean = 24) were much higher than ratios of organic carbon to total nitrogen $(C_{org}/N_{tot})$ (mean= 12), indicating the presence of significant amounts of inorganic nitrogen in the surface sediments of both lakes. Stable isotope ratios for organic nitrogen were, on average, $5.2\%_{\circ}$ heavier than ratios of inorganic nitrogen in Lake Shihwa, but those same ratios were comparable in Lake Hwaong. This might be due to differences in the origin or the degree of degradation of sedimentary organic matter between the two lakes. In addition, stable isotope ratios for labile organic carbon were, on average, $1.4\%_{\circ}$ heavier than those for residual organic carbon, reflecting the preferential oxidation of $^{13}C$-enriched organic matter. The present study demonstrates that KOBr-KOH treatment of sedimentary organic matter can provide valuable information for understanding the origin and degradation state of organic matter in marine and brackish sediments. This also suggests that the ratio of $(C_{org}/N_{org})$ and stable isotope ratios for organic nitrogen can be used as indexes of the degree of degradation of organic matter.

• 생태와환경
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• 제38권spc호
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• pp.8-11
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• 2005

Carbon stable isotope ratios of producers varied in lake ecosystems. In tile present study, we tried to estimate the seasonal variations of carbon isotope ratios of phytoplankton and benthic diatoms in a strongly acidic lake ecosystem. Lake Katanuma is a volcanic, strongly acidic lake (average pH of 2.2), located in Miyagi, Japan. Only two algal species dominate in Lake Katanuma; Pinnularia acidojaponica as a benthic diatom, and Chlamydomonas acidophila as a green alga. Carbon isotope values of P. acidojaponica varied seasonally, while those of particulate organic matter, which were mainly composed of C. acidophila remained fairly stable. The differences suggested that $CO_2$ gas was more frequently limited for P. acidojaponica than C. acidophila, since high density patches of benthic diatoms were sometimes observed on the lake sediment. Generally, carbon concentration mechanisms (CCMs)of microalgae can fix bicarbonate in lakes, and affect the carbon isotope values of microalgae. While, in Lake Katanuma, CCMs of the microalgae may scarcely function because of high $CO_2$ gas concentration and low pH. This is the reason for low seasonal amplitude of carbon isotope values of phytoplankton relative to those in other lakes.

• 한국해양생명과학회지
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• 제7권2호
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• pp.61-73
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• 2022

안정동위원소 분석 기법(Stable isotope analysis, SIA)은 환경과학, 생태학, 지구생물화학, 법의학, 고고학 등 다양한 연구 분야에 활용되고 있다. 본 총설에서는 수산 및 양식 연구에 안정동위원소 비 분석 기법을 활용하기 위해 필요한 배경 지식을 소개하고자 한다. 특히, 자연값(natural abundance)을 이용하는 연구에 초점을 두었고 원소가 생물의 조직으로 통합되는 과정에서 발생하는 분별작용(동위원소 비의 변화)에 대한 원리와 안정동위원소 비가 유용한 도구로서 어떤 목적으로 생태, 환경학 분야에 이용되는지, 나아가 수산 및 양식 분야에 활용 가능한 예들을 제시하고자 한다. 본 논문을 통한 안정동위원소 분야의 이해로 향후 수산학 및 양식 연구에서 안정 동위원소 비의 다양한 활용이 기대된다.

• 생태와환경
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• 제46권2호
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• pp.175-184
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• 2013

본 연구는 영산강하구역 입자물질 유입원의 시 공간적인 변화를 추정해 보고자 몽탄대교에서 영산강 하구둑에 이르는 영산강하구역 세 정점에서 연 6회에 걸쳐 수 층 부유 물질의 탄소 및 질소 안정동위원소 비를 비교하였다. 모든 정점에서 8월에 강우를 통한 외부기원 유기물의 유입이 증가하였으며, 이 때 암모니아성 질소 및 규산염의 유입 역시 함께 증가하였다. 탄소 안정동위원소 비 측정 결과 11월과 8월에 정점 별 유기물의 기원이 크게 차이 났으며, 안정동위원소 비 값을 이용하여 주요 유입원 추정이 가능하였다. 11월에 모든 정점에서 질소 안정동위원소 비가 무거운 값을 보였고, 인산염의 농도가 가장 높게 나타났는데 이는 축산폐수 및 농경지를 통한 유입이 영향을 준 것으로 판단된다. 또한 다른 정점에 비해 영산강 하구둑에서 모든 조사 기간 동안 비교적 높은 질소 안정동위원소 비를 보였으며, 이를 통해 축산폐수 및 농경지를 통한 유기물 유입이 다른 정점에 비해 높을 것으로 추정된다. 본 연구 결과를 통해 안정동위원소 비 분석은 입자성유기물의 기원을 추정하는데 유용한 방법임을 확인할 수 있었다.

• Journal of Ginseng Research
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• 제42권4호
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• pp.485-495
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• 2018

Background: The geographical origin of Panax ginseng Meyer, a valuable medicinal plant, is important to both ginseng producers and consumers in the context of economic profit and human health benefits. We, therefore, aimed to discriminate between the cultivation regions of ginseng using the stable isotope ratios of C, N, O, and S, which are abundant bioelements in living organisms. Methods: Six Korean ginseng cultivars (3-yr-old roots) were collected from five different regions in Korea. The C, N, O, and S stable isotope ratios in ginseng roots were measured by isotope ratio mass spectrometry, and then these isotope ratio profiles were statistically analyzed using chemometrics. Results: The various isotope ratios found in P. ginseng roots were significantly influenced by region, cultivar, and the interactions between these two factors ($p{\leq}0.001$). The variation in ${\delta}^{15}N$ and ${\delta}^{13}C$ in ginseng roots was significant for discriminating between different ginseng cultivation regions, and ${\delta}^{18}O$ and ${\delta}^{34}S$ were also affected by both altitude and proximity to coastal areas. Chemometric model results tested in this study provided discrimination between the majority of different cultivation regions. Based on the external validation, this chemometric model also showed good model performance ($R^2=0.853$ and $Q^2=0.738$). Conclusion: Our case study elucidates the variation of C, N, O, and S stable isotope ratios in ginseng root depending on cultivation region. Hence, the analysis of stable isotope ratios is a suitable tool for discrimination between the regional origins of ginseng samples from Korea, with potential application to other countries.

• 한국지하수토양환경학회지:지하수토양환경
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• 제27권2호
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• pp.87-98
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• 2022

This study aims to prepare a technical protocol for identifying the source of nitrate in water using nitrogen (δ¹⁵N) and oxygen (δ¹⁸O) stable isotope ratios. The technical processes for nitrate sources identification are composed of site investigation, sample collection and analysis, isotope analysis, source identification using isotope characteristics, and source apportionment for multiple potential sources with the Bayesian isotope mixing model. Characteristics of various nitrate potential sources are reviewed, and their typical ranges of δ¹⁵N and δ¹⁸O are comparatively analyzed and summarized. This study also summarizes the current knowledge on the dual-isotope approach and how to correlate the field-relevant information such as land use and hydrochemical data to the nitrate source identification.

• 한국물환경학회지
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• 제35권6호
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• pp.510-519
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• 2019

The 'Stable Isotope Analysis in R' (SIAR), one of the Bayesian mixing models for stable isotopes, has been proven to be useful for source apportionment of nitrates in rivers. In this study, the contribution ratios of nitrate sources were quantified by using the SIAR based on nitrogen and oxygen stable isotope measurements in the Yeongsan River. From the measurements, it was found that the values of δ¹⁵N-NO₃ and δ¹⁸O-NO₃ ranged from -8.2 ‰ to +13.4 ‰ and from +2.2 ‰ to +9.8 ‰, respectively. We further analyzed the contribution ratios of the five nitrate sources by using the SIAR. From the modeling results, the main nitrate source was found to be soil N (29.3 %), followed by sewage (26.7 %), manure (19.6 %), chemical fertilizer (17.9 %) and precipitation (6.3 %). From the results, it was found that the anthropogenic sources, i.e., sewage, manure and chemical fertilizer contribute 64.2% of the total nitrate inflow from the watershed. Due to the significant correlation of δ¹⁵N-NO₃ and lnNO₃^- in this study, the fractionation factors reflecting the biogeochemical processes of stable isotope ratios could be directly obtained. This may make the contribution ratios obtained in this study more precise. The fractionation factors were identified as +3.64 ± 0.91 ‰ for δ¹⁵N-NO₃ (p<0.01) and -5.67 ± 1.73 ‰ for δ¹⁸O-NO₃(p<0.01), respectively, and were applied in using the SIAR. The study showed that the stable isotope method using the SIAR could be applied to quantitatively calculate the contribution ratios of nitrate sources in the Yeongsan River.

• Journal of Ginseng Research
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• 제41권2호
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• pp.195-200
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• 2017

Background: The natural ratios of carbon (C), nitrogen (N), and sulfur (S) stable isotopes can be varied in some specific living organisms owing to various isotopic fractionation processes in nature. Therefore, the analysis of C, N, and S stable isotope ratios in ginseng can provide a feasible method for determining ginseng authenticity depending on the cultivation land and type of fertilizer. Methods: C, N, and S stable isotope composition in 6-yr-old ginseng roots (Jagyeongjong variety) was measured by isotope ratio mass spectrometry. Results: The type of cultivation land and organic fertilizers affected the C, N, and S stable isotope ratio in ginseng (p < 0.05). The ${\delta}^{15}N_{AIR}$ and ${\delta}^{34}S_{VCDT}$ values in ginseng roots more significantly discriminated the cultivation land and type of organic fertilizers in ginseng cultivation than the ${\delta}^{13}C_{VPDB}$ value. The combination of ${\delta}^{13}C_{VPDB}$, ${\delta}^{15}N_{AIR}$, or ${\delta}^{34}S_{VCDT}$ in ginseng, except the combination ${\delta}^{13}C_{VPDB}-^{34}S_{VCDT}$, showed a better discrimination depending on soil type or fertilizer type. Conclusion: This case study provides preliminary results about the variation of C, N, and S isotope composition in ginseng according to the cultivation soil type and organic fertilizer type. Hence, our findings are potentially applicable to evaluate ginseng authenticity depending on cultivation conditions.

• 한국물환경학회지
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• 제36권2호
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• pp.116-124
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• 2020

This study aims to identify the characteristics of organic matter and pollutant sources using water quality and stable isotope ratios (δ¹⁵N, δ¹³C) of the two inflow tributaries (Gulpo (GP) and Anyang (AY) streams). Water samples were analyzed in June and September 2018, and the results showed that the concentrations of nutrients, such as TN and NO₃-N, were increased at GP4, which is located at the downstream of sewage treatment facilities(STFs). TOC and TN ratios showed a strong positive correlation (R² = 0.77, p<0.01) at all points except for GP4. The results of GP's stable isotope ratio analysis do not appear to be a constant cluster compared to AY because GPs with large amounts of pollutants from the industry (metal processing companies, etc.) have less tributary, shorter waterway and significantly different external sources. This could be attributed to different sources of external inflow despite its smaller number of tributaries and shorter waterways than AY. In the first half of the year, the δ¹⁵5N of GP4 was affected by discharge of STFs, while AY3 seemed to have an influence of tributary than the discharge of STFs. Consequently, using water quality, stable isotope ratio and C/N, the sources of contamination in two streams with different contaminants were identified and origin was estimated.

• 생태와환경
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• 제51권1호
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• pp.96-104
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• 2018

안정동위원소비는 전 세계적으로 수생태 환경 연구에서 매우 활발하게 적용되고 있는 분야로써, 국내에서도 2000년대 이후 담수 및 연안 생태계의 먹이망 구조 연구 등 다양한 연구에서 활용되고 있다. 최근에는 기존의 총 조직내의 안정동위원소 분석기법의 한계점을 인지하고 보완하기 위한 방안으로 아미노산의 질소 안정동위원소 분석 기법이 개발되었으며, 이를 활용한 다양한 연구 사례들이 보고됨에 따라 수생태 연구의 활용범위가 급격히 확대되고 있다. 아미노산의 질소 안정동위원소 분석기법의 기술적인 접근성의 한계에 의해 현재까지 국내에서 수생태계 연구를 위해 아미노산의 질소 안정동위원소비를 활용한 연구는 제한적인 실정이지만, 국내 다양한 하천 및 호수 그리고 연안의 환경 변화 특성을 고려할 때, 향후 수생태 환경연구에 있어 그 유용성과 활용 가치가 대단히 높을 것으로 전망된다.