• 대한미생물학회지
• /
• 제11권1호
• /
• pp.27-32
• /
• 1976

Sodium amylosulfate(SAS) has been reported to be an effective substance to inactivate the anti-bacterial activity of blood in blood culture media. The advantage of the use of SAS over sodium polyanethol sulfonate(SPS) is that it does not inhibit the growth of some bacteria! species which are known to be inhibited by SPS. As to S. typhi, SPS is reported to enhance the growth, however the effect of SAS on this organism is not known as yet. Using 43 strains of S. typhi, isolated from clinical materials, the authors tried to determine the effect of SAS on this organism. The methods used for this study were : the SPS and SAS paper disk I sensitivity test, tests on the growth in trypticase soy broth(TSB) with SPS and with SAS, and experimental blood culture in SPS and SAS incorporated TSB. The following results were obtined. 1). S. typhi strains with the turbidity of No. 0.5 tube of MacFarland nepherometer were inoculated onto Mueller-Hinton plate and 1mg disk of SPS and SAS were applied. After 24-hour incubation, none of the 43 strains showed inhibition zone by SPS disk, but all of them showed zones by SAS disk with a mean zone diameter of 9.5mm(Table 1). 2) Inocula consisting of one to 54 viable counts of 37 strains were inoculated into three different media; TSB with 0.05% SPS, TSB with 0.05% SAS and TSB alone. After 24-hour incubation the mean of the optical densities of each medium were 0.483, 0.482 and 0.459 respectively, showing that SAS does not inhibit the growth of S. typhi. Moreover it was shown that there was no correlation between the amount of inocula and growth(Table 2 and Fig. 1). 3). Each set of media in 5 ml amounts consisting of one tube of TSB with 0.05% SPS, one tube of TSB with 0.05% SAS and two tubes of TSB were inoculated with 8, 64. 640 and 6400 viable counts of bacteria. Then 0.5 ml of fresh normal blood was added to all tubes except for one tube of TSB. Macroscopic observation after 24 hour incubation showed a heavy growth in all tubes except for the tube of TSB plus blood, which showed only a light growth in the tube of the heaviest inoculum. This result clearly demonstrates that the growth of S. typhi is inhibited by some antibacterial activities of fresh blood, which are counter acted by SPS and SAS(Table 3). Between SPS and SAS, there was no significant difference found(Table 4 and Fig. 2). With all these results it can be postulated that the addition of SAS into a rountine blood culture media may raise the positivity of S. typhi isolation and shorten the incubation period.

• 한국식품영양학회지
• /
• 제28권1호
• /
• pp.47-59
• /
• 2015

In this study, we investigated the dietary habits, snacks, and self-purchasing snacks (SPS) intake behaviors of 519 elementary school students (boys=239, girls=280). Obesity was significantly higher (p<0.05) in boys (24.8%) than in girls (14.7%) and the proportion of underweight subjects was higher compared to normal or other weight groups for both the boys and the girls. There were 7.5% of the subjects in the group that always skipped breakfast, and the main reason of skipping breakfast was insufficient time (51.9%). The snack intake frequency was once or twice per week for 23.1% of the subjects and three or four times per week for 25.1%. The SPS intake frequency was the subjects zero for 35.6% of the highest level of the responders, while 6.8% of the respondents took more than once SPS per day. 59.6% of the respondents consumed SPS due to hunger while 15.0% consumed SPS out of boredom. The SPS was purchased from supermarkets in 34.5% of the cases, from convenience stores in 24.1% and from snack corners in 20.0% of cases or from a store near school in 14.5% of the cases. Analysis of SPS behaviors according to obesity index showed that parent's opinion of 'permission to buy SPS as needed' had a significant effect in 64.5% over weight subjects compared to only 53.7% in underweight groups. The subjects who used more than 3/4 of their pocket money to buy SPS was higher in the overweight groups (16.4%) than in the underweight groups (7.0%) and normal weight groups (9.8%). The favorite snacks and SPS were milk and yogurt for 45.7% of the subjects, fruits for 42.7%, ice cream for 26.4%, fruit juices for 23.8%, sweet stuff for 16.4%, frozen dessert for 8.9%, and chocolate or candy for 8.1% in descending order. The intake frequency of milk, yogurt, and fruit juices was higher in the underweight groups, but the intake frequency of sweet stuff, frozen dessert, and chocolate or candy was higher in over weight groups. The intake of frozen dessert was more than four times higher in the overweight groups than in the underweight groups. In conclusion, dietary habits, snacks, and SPS intake behaviors were similar between the boys and the girls and obesity groups, but most students appeared to have a high preference for intake snacks and SPS. Therefore, education for appropriate snacks intake habits will be beneficial for improving their dietary habits and health.

• 통상정보연구
• /
• 제13권3호
• /
• pp.403-431
• /
• 2011

환경 관련 문제에 대한 관심이 고조됨에 따라, 국민의 건강 및 생명 보호를 위한 각국의 SPS조치는 국가 간 자유무역이라는 WTO의 주요원칙에 역행하는 규제적인 무역관행을 낳고 있다. 그러나 분쟁 발생 시 패널이 회원국의 조치를 심사하는데 필요한 심사기준과 관련된 규정은 SPS협정에 존재하지 않는다. 뿐만 아니라 과학적 실험, 데이터 등과 밀접한 관련이 있는 협정의 특성으로 인하여, 본 협정에 적합한 심사기준에 대해서는 상반된 의견들이 대립하고 있으며 실제 분쟁사례에서도 일관된 기준이 적용되고 있지 못하다. 국가차원에서 결론 내려진 사실적, 법적 판단을 패널이 심사할 때 적용하는 심사기준은, 당국의 결정을 패널이 존중하는 정도에 따라 '적극적 개입(de novo review) 기준'과 '존중(deference) 기준'이라는 두 가지의 대립적인 기준으로 나누어질 수 있다, 이 두 가지 대립적인 기준을 중심으로, 본 논문에서는 WTO협정에서 규정하고 있는 심사기준을 검토하고 SPS 협정에 적합하게 적용할 수 있는 심사기준을 모색하고자 한다. 그리고 최근 US-Hormones Suspension 사건 판결에서 적용한 심사기준을 분석하여 SPS협정에 적용 가능한 심사기준이 어떠한 방향으로 정립되고 있는지 살펴보고자 한다.

• 한국식생활문화학회지
• /
• 제20권5호
• /
• pp.594-602
• /
• 2005

The purpose of this study was to investigate eating behaviors related to snack and self-purchasing snack (SPS) among elementary school children. Self-administered questionnairs were completed by 352, 5th and 6th grade elementary school students living in 3 different regions which included apartment region in Bundangn, Sungnam (apartment group, n=116), residences in Seodaemun-Gu and Mapo-Gu, Seoul (kang-buk group, n=103) and residence in industrial region in Sungnam (industry group, n=133). The results were as follows: A significantly higher proportion (64.7%) of the apartment group had breakfast every morning than those of kang-buk (48.6%) or industry (52.1%) group (p<0.01). As for the frequency rate of snack and self-purchasing snack (SPS), 53.9% of the subjects answered taking snack more than once per day, 22.8%, once for few days and 23.3%, almost not. However, 15.5% of the subject had SPS once or more per day, 30.7%, 1-2 times per week and 22.4%, almost not. Those of apartment group showed significantly lower SPS frequencies (p<0.01), since higher proportions answered having SPS 1-2times per week (40.9%) and almost not (31.3%) compared to other groups. The reasons for having SPS turned out to be 'hunger' 54.7%, 'being habitual', 15.9%, 'bing bored', 15.7% and 'with peers' 13.7%. When subjects selected SPS foods, they considered taste (31.5%), price (23.0%), mood at the time (14.1%), sanitorial aspect (10.2%) and quantity (10.1%) rather than nutritional aspect (7.2%). Subjects' pocket money was estimated as 3736 won per week and SPS expense per time as 706 won. But subjects who spent more than 2000 won for SPS expense were significantly higher (33.0%) in apartment group than those of other groups (p<0.01). The favorite snack items that subjects having at home were fruit, ice cream, milk and yoghurt, cookies, ramen and bread in order. And favorite SPS items turned out to be ice cream, cookies, duckbokki, frozen bars, gum, chocholate and candy in order. The frequency rate of SPS were evaluated to be significantly related by several variables: those living in apartment area (p<0.01), those taking breakfast regularly (p<0.01), those of normal weight status by Rohrer index (p<0.05) and those receiving less pocket money (p<0.01) showed lower SPS frequency rate.

• 대한미생물학회지
• /
• 제9권1호
• /
• pp.13-18
• /
• 1974

Blood is one of the most important clinical specimens for the isolation of bacteria. A rapid isolation and a high isolation rate of bacteria are very important in blood culture because bacteremic patients are mostly in grave condition. Various blood culture media which support growth of most fastidious bacteria are available commercially. However, growth of bacteria are frequently delayed because of antibacterial activity of blood. Sodium polyanethol sulfonate(Liquoid) has been reported to inactivate the antibacterial substance and disrupt phagocytic cells. The beneficial effect of SPS is well recognized in the isolation of gram-positive bacteria. However, the effect does not seem to be prominent for gram-negative bacilli isolation mainly due to the rapidity of their growth. It has been experienced with Sal. typhi that the growth is much slower than that of other gram-negative bacilli. For the rapid growth of the organism, use of bile broth has been recommended. Although Sal. typhi is the most frequently isolated organism at present, about one half of total isolates are other organisms and, in case bile broth is used, other media which support growth of these organisms should be used together. Fluid thioglycollate medium(FTM) which is always used in blood culture to isolate anaerobes is inferior to brain heart infusion(BHI) for the isolation of aerobes. This study was done to determine the effect of SPS on the isolation of Sal. typhi from blood. During the Sep. 1973 to Sep. 1974 study period, 2460 blood cultures were made from the Severance hospital patients: BHI and FTM sets 1431 specimens, BHI with SPS(0.05%) and FTM sets 396 specimens, BHI and FTM with SPS sets 359 specimens, BHI and BHI with SPS sets 274 specimens. Mean incubation time required for the macroscopic detection of growth of Sal. typhi were 3.5 days on BHI and 2.7 days on BHI with SPS. The 0.8 day difference was statistically significant. On FTM the mean incubation time was 3.8 days while it was 2.9 days on FTM with SPS. The 0.9 day difference was statistically significant. The result on BHI with and without SPS sets showed faster growth on BRI with SPS in 7 specimens and slower growth in one specimen and the remaining 12 showed growth at the same time. These specimens had mean incubation time of 3.2 days on BHI and 2.3 days on BHI with SPS. The 0.9 day difference was statistically significant. This study indicates beneficial effect of SPS for the rapid isolation of Sal. typhi from clinical blood specimens.

• 대한감각통합치료학회지
• /
• 제14권2호
• /
• pp.11-21
• /
• 2016

목적 : 본 연구의 목적은 국내에서 개발된 아동감각처리척도(Sensory Processing Scale for Children: SPS-C)의 구성타당도와 내적일치도를 검증하는 것이다. 연구방법 : 2014년 11월부터 2016년 6월까지 감각처리 문제가 있는 만 3~5세 아동 117명을 대상으로 SPS-C 평가를 시행한 자료를 바탕으로 요인 분석과 내적 일치도 분석을 시행하였다. 결과 : 요인분석 결과, 청각, 시각, 촉각 영역은 각각 높은 요인 부하량으로 묶였으며, 전정감각과 고유수용성감각은 두 영역의 요인 부하량이 중복되는 경향으로 나타났다. 내적 일치도 검증 결과 전체 척도의 내적 일치도는 .728이었으며, 각 영역별 내적 일치도는 .323~.636 수준의 신뢰도를 보였다. 결론 : 본 연구 결과를 바탕으로 SPS-C의 타당도와 신뢰도가 검증되었으며, 추후 연구에서는 다양한 타당도와 신뢰도 검증 및 표준화 연구가 이루어져야 할 것이다.

• 미생물학회지
• /
• 제41권1호
• /
• pp.8-12
• /
• 2005

S. chungbukensis DJ77의 genome project수행 결과 다당류 생 합성 에 관련된 유전자들의 염기서열을 찾아내었다. 본 논문에서는 이러한 유전자들 중 sphigan형 다당류 생합성에 관여하는 glucosyl-isoprenyl phosphate-transferase를 암호화 하는 유전자의 완전한 서열을 결정하였고, spsB로 명명하였다. 이 유전자는 ATG를 개시코돈으로 사용하며, TGA를 종결코돈으로 사용하고 있다. 또한 총 1392 bp의 open reading frame을 포함하며, 463개의 아미노산으로 구성되어있다. SpsB를 구성하는 아미노산 서열은 동일한 속의 sphingan 형성 균주인 Sphingomonas spp S88의 SpsB와 $50\%$, Sphingomonas paucimobilis ATCC 31461의 GelB와 $48\%$의 유사성을 나타내었다.

• 동의생리병리학회지
• /
• 제26권1호
• /
• pp.35-39
• /
• 2012

Sweet potato soju(SPS) has been made by vacuum distillation because sweet potato contains much fibrous materials which give high density to sweet potato mash. Generally, the SPS made by vacuum distillation has soft flavors and tastes. If the viscosity of sweet potato mash could be decreased by degradation enzyme, the process and production of SPS making by the method of vacuum distillation may be simplified and easier to distil the fermented sweet potato. Because the fibrous materials of sweet potato contains pectin with methoxyl group, methanol can be produced by fibrous degradation enzyme. For appling the fiber degradation enzymes to sweet potato mash for making SPS, the enzyme should be needed to degrade fibrous material without producing methanol. Special two fibrolytic enzymes are selected from 26 kind of commercial enzymes for the simplified and easier production of sweet potato soju by vacuum distillation, The selected enzyme A and X can degrade the fibrous material pectin of sweet potato without producing methanol. Although the different companies have produced the enzymes, same cellulase has been prepared from Trichoderma. reesei. The viscosity of sweet potato mash treated by the enzymes is decreased by 3 times with comparison to the viscosity of sweet potato mash of control group. The methanol concentration in the vacuum distilled SPS treated with the enzymes is 0.16%. The concentration is similar to that of commercially distilled SPS(0.15%). The result may suggest that the selected cellulases, A and X, can be used to make SPS by vacuum distillation.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2003년도 하계학술대회 논문집 A
• /
• pp.224-226
• /
• 2003

Design of a GA-based SPS controller for Power system stabilization has been investigated in this paper. The design problem of SPS controller is formulated as an optimization problem using GA. The dynamic characteristic responses are considered to verify performance of the proposed SPS under various disturbance for various operation conditions. The simulation results show that the proposed SPS controller provides most of the damping and improves greatly the voltage profile of the system under two different disturbances.

• 대한미생물학회지
• /
• 제22권2호
• /
• pp.103-108
• /
• 1987

Vibrio vulnificus septicemia is nor-rare diease in Korea. Carriage rate of the orgaism by shellfish is not well known. In this study performance of SPS agar and SGP broth and effect of storage of specimen in the isolaton was determined using the shellfish specimens collected from the coast and market of Koonsan city. Isolation rate was similar with TCBS and with SPS, but the rate became much highher after enrichment in SGP broth. 80% of oyster speimes were positve when inoculated immediately, but the rate dropped rapidly after storage of specimens at freezing temperature for sometime. All of the isolates fermented lactose in 2 days. A few isolates were not identfiable with API 20E system, because of acid prduction from melibiose. Serover 04 was the frequent isolates.