The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Effect of Use of SPS Agar and SGS Broth and Storage of Specimen on the Isolation of Vibrio vulnificus

Vibrio vulnificus 분리율에 대한 SPS Agar와 SGP Broth의 사용 및 검체 저장의 영향

  • Chong, Yun-Sop (Department of Clinical Pathology, Yonsei University College of Medicine) ;
  • Lee, Samuel Y. (Department of Clinical Pathology, Yonsei University College of Medicine) ;
  • Kim, Shin-Moo (Wonkwang Junior College)
  • 정윤섭 (연세대학교 의과대학 임상병리학교실) ;
  • 이삼열 (연세대학교 의과대학 임상병리학교실) ;
  • 김신무 (원광보건전문대학)
  • Published : 1987.06.30
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Abstract

Vibrio vulnificus septicemia is nor-rare diease in Korea. Carriage rate of the orgaism by shellfish is not well known. In this study performance of SPS agar and SGP broth and effect of storage of specimen in the isolaton was determined using the shellfish specimens collected from the coast and market of Koonsan city. Isolation rate was similar with TCBS and with SPS, but the rate became much highher after enrichment in SGP broth. 80% of oyster speimes were positve when inoculated immediately, but the rate dropped rapidly after storage of specimens at freezing temperature for sometime. All of the isolates fermented lactose in 2 days. A few isolates were not identfiable with API 20E system, because of acid prduction from melibiose. Serover 04 was the frequent isolates.

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