Two mosquito species were collected in still-water near farming area in Andong, Korea. Based on morphological characters, these two mosquitoes were identified as Aedes koreicus and Culex vagans, respectively. DNA barcode analyses supported the identification. An entomopathogenic bacterium, Bacillus thuringiensis subsp. israelensis (BtI), exhibited insecticidal activities against the two mosquito species and its virulence was more potent than that of B. thuringiensis subsp. kurstaki. It has been known that the bacterial metabolites of Xenorhabdus spp. suppress insect immunity and enhance pathogenicity of B. thuringiensis. This study tested the effect of the bacterial culture broth of Xenorhabdus spp. on enhancing BtI pathogenicity. Among three Xenorhabdus spp., culture broth of X. ehlersii (Xe) was relatively effective to enhance BtI pathogenicity against both mosquito species. Indeed, organic extracts of Xe culture broth suppressed the hemocyte-spreading behavior, suggesting the presence of immunosuppressant in the culture broth. These results suggest a formulation of BtPlus by mixing BtI spore and Xe culture broth to be applied to control the two mosquito species.
The occurrence of Rice stripe virus (RSV) has been confirmed in some parts of South Korea. In the current year, this was observed to be limited in the double cropping zones of the southern region. Earlier, RSV occurred largely in the west coast regions of the country, particularly in Gangwha, Gyeonggi-do in 2001; and Buan, Jeollabuk-do and Seocheon, Chungcheongnam-do in 2007. This study was carried out to determine the nationwide extent of RSV occurrence by investigating RSV infestations in different latitudes and altitudes. Result revealed a recent reported occurrence of RSV in the southern region (Milyang, latitude $35^{\circ}$), a reported occurrence in the northern region (Cheolwon, latitude $38^{\circ}$) in 2005, and in the middle northern region of the east coast (Goseon, latitude $38^{\circ}$) as newly confirmed from this investigation. Results also confirmed the occurrence of RSV in a wide range of altitude from the plains (Milyang, 17 m altitude) to the alpine regions (Jinbu, 576 m) including the middle mountains (Sangju, altitude 285 m). The RSV occurrence in Jinbu in the alpine region and at Goseong and most of the northern end regions were confirmed only this year. The results of the present study confirmed that RSV is a fast-spreading disease in rice plant cultivation regions of the entire country.
Entomopathogenic bacteria (Xenorhabdus nematophila, X. sp. and Photorhabdus temperata subsp. temperata) isolated from entomopathogenic nematodes express potent insecticidal activity in insect hemocoel. They are also known to suppress insect immune mediation by inhibiting phospholipase $A_2$, leading to host immunosuppression. This study analyzed effects of their cultured broths on inhibiting insect immunosuppression. For this, we removed all bacterial cells using $0.2\;{\mu}m$ pore sized membrane from the bacteria-cultured broth. All three sterilized cultured media, in dose-dependent manners, significantly inhibited hemocyte-spreading behavior of 5th instar larvae of Spodoptera exigua. However, they showed differential inhibitory activities among different bacterial species, in which X. nematophila showed the most potent inhibitory activity. This immunosuppressive effect was applied to increase the pathogenicity of Bacillus thuringiensis (Bt). All three bacterial cultured broths including bacterial cells significantly potentiated Bt pathogenicity against young S. exigua larvae when each of them was orally administered in a mixture of low dose of Bt. Finally, we tested the effect of oral administration of the cultured media containing the immunosuppressive compound(s) secreted by the bacteria. The membrane-sterilized cultured broths were mixed with the low dose of Bt and then orally administered to the young S. exigua. Only the cultured medium of X. nematophila showed increase of Bt pathogenicity. These results indicated that the; cultured media of the three bacteria possessed immunosuppressive factor(s), which may act to potentiate Bt toxicity to young S. exigua larvae.
Kim, Young-Ha;Kil, Ji-Hyon;Hwang, Sun-Min;Lee, Chang-Woo
Weed & Turfgrass Science
/
v.2
no.2
/
pp.138-151
/
2013
This study was carried out to investigate the distribution and habitat types of prickly lettuce, Lactuca scariola of Europe origin for supplying the basic data of management plan. It showed fairly nation-wide distribution although excessive growth was rather limited in the wild. Its habitat types were divided into four types like open fields, roadsides, seashores and riversides. Species diversity examined by species rank-dominance curve tended to increase over riverside > seashores > open fields > roadsides. As a result of analyzing life form, therophytes were more than 50%, means that the habitats of L. scariola were disturbed by human activities etc. Urbanization Index was analyzed 9.1% in roadsides, 7.4% in seashores, 5.8% in open fields and riversides. It has high spread potential with a large number of wind-flying seed per plant. It was evaluated that prickly lettuce was mainly spread along the newly constructed road, expressway and invaded the original ecosystem in the cultivated land as weeds. But it has played a role as a pioneer species in open fields. It is recommended to remove where it has considerable impact on the native plant species of conservation value.
Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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v.39
no.4
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pp.211-221
/
2021
The Rabbit grass(Trifolium Repens, call it 'Clover') is a representative harmful plant of lawn, and it starts growing earlier than lawn, forming a water pipe on top of the lawn and hindering the photosynthesis and growth of the lawn. As a result, in competition between lawn and clover, clover territory spreads, but lawn is damaged and dried up. Damage to the affected lawn area will accelerate during the rainy season as well as during the plant's rear stage, spreading the area where soil is exposed. Therefore, the restoration of damaged lawn is causing psychological stress and a lot of economic burden. The purpose of this study is to distinguish clover which is a representative harmful plant on lawn, to identify the distribution of damaged areas due to the spread of clover, and to review of changes in vegetation before and after the eradication of clover. For this purpose, a time series analysis of three vegetation indices calculated based on images of convergence Drone with RGB(Red Green Blue) and BG-NIR(Near Infra Red)sensors was reviewed to identify the separation between lawn and clover for selective eradication, and the distribution of damaged lawn for recovery plan. In particular, examined timeseries changes in the ecology of clover before and after the weed-whacking by manual and brush cutter. And also, the method of distinguishing lawn from clover was explored during the mid-year period of growth of the two plants. This study shows that the time series analysis of the MGRVI(Modified Green-Red Vegetation Index), NDVI(Normalized Difference Vegetation Index), and MSAVI(Modified Soil Adjusted Vegetation Index) indices of drone-based RGB and BG-NIR images according to the growth characteristics between lawn and clover can confirm the availability of change trends after lawn damage and clover eradication.
Ha, Sung-Won;Kim, Tae-Won;Choi, Joo-Hwan;Park, Young-Jin
Journal of the Korea Academia-Industrial cooperation Society
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v.20
no.4
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pp.580-589
/
2019
Indonesia has a very short supply of electricity. As a solution to this problem, plans for construction of thermal power plants are increasing. Thermal power plant require the cooling water system to cool the overheated engine and equipment that accompany power generation, and the circulation water pump chamber among the cooling water system are generally designed according to the ANSI (1998) standard. In this study, the design criterion $20^{\circ}$ for the spreading angle of the ANSI (1998) of the layout of the circulating water pump chamber can not be satisfied on the K-coal thermal power plant site condition in Indonesia. Therefore, 3-D numerical model experiment was carried out to obtain a hydraulically stable flow and stable structure. The Flow-3D model was used as numerical model. In order to examine the applicability of the Flow-3D model, the flow study results around the rectangular structure of Rodi (1997) and the numerical analysis results were compared around the rectangular structures. The longitudinal velocity distribution derived from numerical analysis show good agreement. In order to satisfy the design velocity in the circulating water pump chamber, a rectangular baffle favoring velocity reduction was applied. When the approach velocity into the circulating water pump chamber was occurred 1.5 m/s ~ 2.5 m/s, the angle of the separation flow on the baffle was occurred about $15^{\circ}{\sim}20^{\circ}$. By placing the baffle below the separation flow angle downstream, the design velocity of less than 0.5 m/s was satisfied at inlet bay.
KANG, Eun Su;KIM, Han Gyeol;NAM, Myoung Ja;CHOI, Mi Jung;SON, Dong Chan
Korean Journal of Plant Taxonomy
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v.52
no.3
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pp.184-195
/
2022
The invasive alien species Brassica tournefortii Gouan (Brassicaceae) is herein reported for the first time in Korea, from Gunsan-si, Gochang-gun, and Jeju-si. Brassica tournefortii can easily be distinguished from B. juncea and B. napus by its dense stiff hairs at the base of the stem and leaves, basally and distally branched stems, partially dehiscent fruits, and seeds that become mucilaginous in the presence of moisture. Although some taxonomists have classified this species as belonging to Coincya Rouy based on its fruit and seed characteristics, the existence of one vein on the fruit valves and our maximum likelihood analysis using internal transcribed spacer sequences placed it in Brassica. Distribution data, photographs, and a description of B. tournefortii are presented herein. Moreover, potential changes in the distribution of B. tournefortii were predicted under different climate scenarios, but our analysis showed that the probability of the spreading of this species is low. Nevertheless, continuous monitoring is necessary for an accurate assessment. The results of the present study can be used to conduct an invasion risk assessment and can assist with the effective management of this invasive alien species.
Hydrogen energy is emerging as an important means of carbon neutrality in the various sectors including power, transportation, storage, and industrial processes. Fuel cell power plants are the fastest spreading in the hydrogen ecosystem and are one of the key power sources among means of implementing carbon neutrality in 2050. However, high volatility in system marginal price (SMP) and renewable energy certificate (REC) prices, which affect the profits of fuel cell power plants, delay the investment timing and deployment. This study applied the real option methodology to analyze how the dual uncertainties in both SMP and REC prices affect the investment trigger price level in the irreversible investment decision of fuel cell power plants. The analysis is summarized into the following three. First, under the current Renewable Portfolio Standard (RPS), dual price uncertainties passed on to plant owners has significantly increased the investment trigger price relative to one under the deterministic price case. Second, reducing the volatility of REC price by half of the current level caused a significant drop in investment trigger prices and its investment trigger price is similar to one caused by offering one additional REC multiplier. Third, investment trigger price based on gray hydrogen and green hydrogen were analyzed along with the existing byproduct hydrogen-based fuel cells, and in the case of gray hydrogen, economic feasibility were narrowed significantly with green hydrogen when carbon costs were applied. The results of this study suggest that the current RPS system works as an obstacle to the deployment of fuel cell power plants, and policy that provides more stable revenue to plants is needed to build a more cost-effective and stable hydrogen ecosystem.
Kim, Ye Eun;Kim, Jun Young;Noh, Hyeong Jin;Lee, Dong Hyeung;Kim, Su San;Kim, Seong Hwan
Korean Journal of Environmental Agriculture
/
v.38
no.4
/
pp.269-272
/
2019
BACKGROUND: Since 2015, fire blight disease caused by Erwinia amylovora has been devastating apple and pear orchards every year. To quickly block the disease spreading, infected apple and pear trees have been buried in soil. However, concern on the possibility of the pathogen survival urgently requires informative data on the buried host plants. Therefore, this study was conducted to investigate the survival of the pathogen from the buried host plants. METHODS AND RESULTS: Apple trees buried in 42 months ago in a Jecheon site and pear trees buried in 30 months ago in an Anseong site were excavated using an excavator. Plant samples were taken from stems and twigs of the excavated trees. The collected 120 samples were checked for rotting and used for bacterial isolation, using TSA, R2A, and E. amylovora selection media. The purely isolated bacteria were identified based on colony morphology and 16S rDNA sequences. Wood rotting and decay with off smells and discoloring were observed from the samples. A total of 17 genera and 48 species of bacteria were identified but E. amylovora was not detected. CONCLUSION: Our investigation suggests that the survival of E. amylovora doesn't seem possible in the infected hosts which have been buried in soil for at least 30 months. Therefore, the burial control can be considered as a safe method for fire blight disease.
Journal of the Korean Society of Environmental Restoration Technology
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v.14
no.1
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pp.89-104
/
2011
This study was carried out to the development of ecological planting model to make up of coastal windbreak forest on the Suncheon-bay in Sucheon-si, Korea. Make up of coastal windbreak forest in this site was needed for appropriate bioresource, biodiversity and ecological structure, and for conservation of the eco-tour resource and protection of human life and property by the unforeseen disaster from the coast. Based on the plant-social principle, the planting model of windbreak forest was developed to facilitate growth of trees, considering planting locations. The ecological planting model for the coastal windbreak was composed of warm temperate evergreen and windbreak forest which is spreading around the inland area in Korea. The horizontal forest style was composed of forest edge community and inner forest community, and the vertical forest style was composed of upper, middle, low and ground planting class. The target of the present model was quasi-natural forest, and the species of tree were selected based on the adaptability to surroundings depending on a goal to create a forest and forest style. To achieve both functions of wind break forest and visual effect in short period of time, small trees and seedlings were planted with high-density of 40,000/ha in an expectation of easy natural maintenance in the future. The significance of the present study is a suggestion for a guideline to create ecological coastal windbreak forest in the Suncheon-bay in which the harmony of human life and the ecological conservation is of great importance. Also, the ecological coastal windbreak forest model should be developed further through the long term monitoring after construction of forest.
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