• 한국추진공학회:학술대회논문집
• /
• 한국추진공학회 2008년도 제31회 추계학술대회논문집
• /
• pp.349-352
• /
• 2008

수축-확대 초음속 노즐 내부에 분출된 이차제트에 의한 추력편향 제어에 관한 실험적, 수치적 연구가 진행되었다. 특정위치(노즐 목으로부터 12mm 떨어진 곳)에서 분출되는 이차제트 유동전압이 변화할 때 나타나는 제트유동의 추력편향 특성이 관찰되었다. 수치해석 결과는 동일한 경계조건에서 수행된 과거 연구결과 및 본 연구에서 수행된 쉴러린 유동가시화 결과와 비교되었으며, 정성적으로 좋은 일치를 나타냈다. 추력편향의 특성은 노즐 내부의 경사충격파의 반사구조, 즉, 이차제트 압력비 SPR의 크기에 관계되어 있음이 관찰되었다.

• 한국의학물리학회:학술대회논문집
• /
• 한국의학물리학회 1999년도 Japanese Journal of Medical Physics
• /
• pp.278-281
• /
• 1999

The purpose of this study is to investigate the effect of the scattered x-rays on the subject contrast and image sharpness for various tube voltages. For the purpose, we measured the scatter-to-primary ratio(SPR) for the tube voltages f 50 to 100kV and obtained the tube voltage dependence of the subject contrast of an aluminum plate in a polymethyl methacrylate(PMMA) phantom. Furthermore, the overall modulation transfer functions(MTFs), which consist of MTFs of a screen-film system and scatter FTMs, were obtained for tube voltages of 50 to 100 kV. The subject contrast decreased with the tube voltage due to that the SPR increased with the tube voltage and that the difference in effective linear attenuation coefficients between the object and its surroundings decreased with the tube voltage. The maximum frequency of the overall MTF decreased from about 2 mm$\^$-1/ to 1 mm$\^$-1/ with the tube voltage increasing from 50 to 100 kV.

• 한국분말재료학회지
• /
• 제21권6호
• /
• pp.441-446
• /
• 2014

In this study, we synthesize silica-core gold-satellite nanoparticles (SGNPs) for the surface-enhanced Raman scattering (SERS) based sensing applications. They consist of gold satellite nanoparticles (AuNPs) fixed on the silica core nanoparticles, which sizes of AuNPs can be tunned by varying the amount of reactants (growth solution and reducing agent). Their surface plasmon resonance (SPR) properties were characterized by using UV-vis spectroscopy, showing that the growth of AuNPs on silica cores leads to the light absorption in the longer wavelength region. Furthermore, the size increase of AuNPs exhibited the dramatic change in SERS activity due to the formation of hot spots. The optimized SGNPs showing enhancement factor ${\sim}3.8{\times}10^6$ exhibited a detection limit of rhodamine 6G (R6G) as low as $10^{-8}M$. These findings suggest the importance of size control of SGNPs and their SPR properties to develop highly efficient SERS sensors.

• 센서학회지
• /
• 제24권6호
• /
• pp.423-428
• /
• 2015

A bimetallic chip made of gold and silver was investigated in intensity interrogation mode to confirm enhancement of the SPR sensor resolution. Both reflectance curves of the bimetallic chip and the conventional gold chip was acquired and compared. The line width of the reflectance curve of the bimetallic chip was narrower than that of the conventional Au chip, resulting in steeper tangential slope. The reflectance was monitored at the angle related to the steepest tangential slope. The change in reflectance of the bimetallic chip was larger than that of the Au chip. The critical angle was analyzed by differentiating the reflectance with respect to incident angle twice. Acquiring the critical angle regarding to the sample informs the refractive index of the sample. Using various concentration of Bovine Serum Albumin, we confirmed that refractive index was linearly related to variation of reflectance of the bimetallic chip.

• Animal Bioscience
• /
• 제34권4호
• /
• pp.724-733
• /
• 2021

Objective: The objectives of this study were to investigate the direct antioxidative effect of 90 Kda heat shock protein (Hsp90) obtained from duck muscle. Methods: The interaction of Hsp90 with phospholipids and oxidized phospholipids was studied with surface plasmon resonance (SPR), and their further oxidation in the presence of Hsp90 was evaluated with thiobarbituric acid reactive substances (TBARS) assay. The scavenging effect on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid (ABTS) was measured, and the electron paramagnetic resonance (EPR) spectroscopy in combination with 5-tert-Butoxycarbonyl-5-methyl-1-pyrroline-N-oxide and 2-phenyl-4,4,5,5,-tetramethylimidazoline-1-oxyl-3-oxide (PTIO) was utilized to determine the abilities of Hsp90 in scavenging hydroxyl and PTIO radicals. Results: SPR showed Hsp90 could bind with both phospholipids and oxidized phospholipids, and prevent their further oxidation by the TBARS assay. The DPPH and ABTS scavenging activity increased with Hsp90 concentration, and could reach 27% and 20% respectively at the protein concentration of 50 μM. The EPR spectra demonstrated Hsp90 could directly scavenge ·OH and PTIO· radicals. Conclusion: This suggests that Hsp90, a natural antioxidant in meat, may play an important role in cellular defense against oxidative stress, and may have potential use in meat products.

• 대한전기학회논문지
• /
• 제36권9호
• /
• pp.670-677
• /
• 1987

The robustness and stability properties for a model reference adaptive control system with plant uncertainty are considered in this paper, using input-output stability theory. An error model for a typical adaptive control structure is extended to unmodelled dynamics in the plant model and then, the strictly positive real condition for global stability is examined. In general, since this condition can be easily violated due to unmodelled dynamics, a modified compensator which can be guaranteed Hev e SPR is introduced in the plant model and the effectiveness for the given structure is also given.

• 한국화재소방학회논문지
• /
• 제30권5호
• /
• pp.93-99
• /
• 2016

이 연구에서는 4종의 암모늄염으로 도포된 베니어 시험편의 연소 특성을 시험하였다. 20 wt%의 암모늄염 수용액으로 각각 베니어 시험편에 3회 붓으로 도포하여 실온에서 건조시킨 후, 콘칼로리미터(ISO 5660-1, 2)를 사용하여 연소특성을 조사하였다. 그 결과, 모노암모늄 포스페이트(MAPP)와 디암모늄 포스페이트(DAPP)로 처리한 시험편은 순수한 베니어 시험편에 비해 평균열방출율($HRR_{mean}$)이 각각 6.7%, 10.0% 낮았다. 반면에 MAPP는 최대 일산화탄소발생속도($CO_{peak}$ production rate)가 순수한 베니어 시험편에 비해 15.7% 높았고 DAPP는 8.2% 낮았다. 황산암모늄(AMSF)으로 도포된 베니어 시험편의 최대 연기발생속도($SPR_{peak}$)는 순수한 베니어 시험편에 비해 9.6% 낮았고 DAPP로 처리된 시험편은 33.3% 낮았다. 또한 DAPP는 최대 연기비감쇠면적($SEA_{peak}$)에 다다르는 시간이 순수한 베니어 시험편에 비해 38.4% 지연되었다. 그러므로 DAPP는 베니어의 연소성질을 억제시키고 연기발생을 감소시키는 경향이 나타났다.

• 한국건축시공학회:학술대회논문집
• /
• 한국건축시공학회 2013년도 춘계 학술논문 발표대회
• /
• pp.65-66
• /
• 2013

As a result of executing Cone Calorimeter experiment on 12 samples among combustibles of domestic residential facilities, flooring materials showed higher HRR and THR than wall papers, and in case of toxicity and SPR, wall papers having adhesive components in one side by considering use conveniences were measured high.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제10권4호
• /
• pp.309-314
• /
• 2005

We performed a basic experiment for the rapid, on-line, real-time measurement of hepatitis B surface antigen using a surface plasmon resonance biosensor. We immobilized anti­HBsAg (hepatitis B surface antigen) polyclonal antibody, as a ligand, to the dextran layer on a CM5 chip surface that had previously been activated by N-hydroxysuccinimide. A sample solution containing HBsAg was fed through a microfluidic channel, and the reflecting angle change due to the mass increase from the binding was detected. The binding characteristics between HBsAg and its polyclonal antibody followed the typical monolayer adsorption isotherm. When the entire immobilized antibody had interacted, no additional, non-specific binding occurred, suggesting the immunoreaction was very specific. The bound antigen per unit mass of the antibody was independent of the immobilized ligand density. No significant steric hindrance was observed at an immobilization density of approximately $17.6 ng/mm^2$. The relationship between the HBsAg concentration in the sample solution and the antigen bound to the ligand was linear up to ca. $40{\mu}g$/mL. This linearity was much higher than that of the ELISA method. It appeared the anti­gen-antibody binding increased as the immobilized ligand density increased. In summary, this study showed the potential of this SPR biosensor-based method as a rapid, simple and multi­sample on-line assay. Once properly validated, it may serve as a more efficient method for HBsAg quantification for replacing the ELISA.

• Journal of Microbiology and Biotechnology
• /
• 제18권1호
• /
• pp.145-152
• /
• 2008

A cell-based in vitro exposure system was developed to determine whether oxidative stress plays a role in the cytotoxic effects of volatile organic compounds (VOCs) such as benzene, toluene, xylene, and chlorobenzene, using human epithelial HeLa cells. Thin films based on cysteine-terminated synthetic oligopeptides were fabricated for immobilization of the HeLa cells on a gold (Au) substrate. In addition, an immobilized cell-based sensor was applied to the electrochemical detection of the VOCs. Layer formation and immobilization of the cells were investigated with surface plasmon resonance (SPR), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The adhered living cells were exposed to VOCs; this caused a change in the SPR angle and the VOC-specific electrochemical signal. In addition, VOC toxicity was found to correlate with the degree of nitric oxide (NO) generation and EIS. The primary reason for the marked increase in impedance was the change of aqueous electrolyte composition as a result of cell responses. The p53 and NF-${\kappa}B $ downregulation were closely related to the magnitude of growth inhibition associated with increasing concentrations of each VOC. Therefore, the proposed cell immobilization method, using a self-assembly technique and VOC-specific electrochemical signals, can be applied to construct a cell microarray for onsite VOC monitoring.