• Parasites, Hosts and Diseases
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• 제48권3호
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• pp.195-201
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• 2010

We studied on the proteomic characteristics of Toxoplasma gondii KI-1 tachyzoites which were originally isolated from a Korean patient, and compared with those of the well-known virulent RH strain using 2-dimensional electrophoresis (2-DE), mass spectrometry, and quantitative real-time PCR. Two-dimensional separation of the total proteins isolated from KI-1 tachyzoites revealed up to 150 spots, of which 121 were consistent with those of RH tachyzoites. Of the remaining 29 spots, 14 showed greater than 5-fold difference in density between the KI-1 and RH tachyzoites at a pH of 5.0-8.0. Among the 14 spots, 5 from the KI-1 isolate and 7 from the RH strain were identified using MALDI-TOF mass spectrometry and database searches. The spots from the KI-1 tachyzoties were dense granule proteins (GRA 2,3,6, and 7), hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGRPTase), and uracil phosphoribosyltransferase (UPRTase). The spots from the RH strain were surface antigen 1 (SAG 1), L-lactate dehydrogenase (LDH), actin, chorismate synthase, peroximal catalase, hexokinase, bifunctional dihydrofolate reductase-thymidylate synthase (DHTR-TS), and nucleosidetriphosphatases (NTPases). Quantitative real-time PCR supported our mass spectrometric results by showing the elevated expression of the genes encoding GRA 2,3, and 6 and UPRTase in the KI-1 tachyzoites and those encoding GRA 7, SAG 1, NTPase, and chorismate synthase in the RH tachyzoites. These observations demonstrate that the protein compositions of KI-1 and RH tachyzoites are similar but differential protein expression is involved in virulence.

• 생약학회지
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• 제15권2호
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• pp.85-90
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• 1984

The whole plant of Duchesnea indica (Andr.) Focke (Rosaceae) which has been used in folklore in treating amenorrhea, inflammation, fever and traumatic injuries, in detoxifying and breaking up clots, was studied. The pharmacological test showed that the water extract had estrogenic and histaminergic actions, but no antiserotonin action. As a result of systematic separation in order to detect the active compounds revealing the estrogenic effect, the active compounds were found in the ether fraction. TLC of the ether fraction revealed 8 spots. Among them three major spots (Rf=0.54, 037, 0.31) were separated by preparative TLC. Some chemical properties of those major spots suggested that they were phenolic compounds, but that they were neither linoleic acid nor ${\beta}-sitosterol$ previously reported.

• Coupled systems mechanics
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• 제1권2호
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• pp.165-182
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• 2012

Macroscopic hot spots formed due to the large thermal gradients at the surface of the disc brake rotor, make the rotor to fail or wear out early. Thermo-elastic deformation results in contact concentration, leading to the non uniform distribution of temperature making the disc susceptible to hot spot formation. The formation of one hot spot event will predispose the system to future hot spotting at the same location. This leads to the complete thermo-elastic instability in the disc brakes; multitude parameters are responsible for the thermo elastic instability. The predominant factor is the sliding velocity and above a certain sliding velocity the instability of the brake system occurs and hot spots is formed in the surface of the disc brake. Commercial finite element package ABAQUS(R) is used to find the temperature distribution and the result is validated using Rowson's analytical model. A coupled analysis methodology is evolved for the automotive disc brake from the transient thermo-elastic contact analysis. Temperature variation is studied under different sliding speeds within the operation range.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2015년도 추계 학술논문 발표대회
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• pp.185-186
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• 2015

The Standard penetration test determines the type of soil according to soil bearing capacity, and this classifies the subsoil into many layers. Construction project managers are willing to know the depth of the present types of subsoil on site in order to make plans on earthwork stage during excavation. However the standard penetration test may not provide accurate information on subsoil type due to incorrect spacing. To solve this problem, this study propose a conceptual algorithm for determining the spacing of standard penetration test spots to essentially tests relevant locations on which to be applied the standard penetration test. This provides the acquirement of the accurate layered model volume of earthwork revised into geological columnar section. This algorithm will determine the appropriate standard penetration test spots spacing on a given size of site to optimize the accuracy of the earthwork volume, time and cost.

• 한국항만학회지
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• 제14권1호
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• pp.87-95
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• 2000

This stability of a plate structure is very crucial problem which results in wrinkle and buckling. In this study, the effect of the pattern of spot-welding points of the two rectangular plates on the compressive and shear buckling load is studied with respect to the thickness, aspect ratio of plates and number of welding spots. Buckling coefficient of the plate not welded was compared with that of two plates with various thickness to extract the effect of thickness. The effect of number of welding spots are studied in two directions, longitudinal and transverse directions. The conclusions obtained were that the reinforcement effect was maximized when the aspect ratio was close to 1.75 at compressive load condition and that the effect of number of welding spots in transverse direction was larger than that in longitudinal direction at shearing load condition.

• 한국산학기술학회:학술대회논문집
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• 한국산학기술학회 2009년도 추계학술발표논문집
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• pp.854-859
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• 2009

Multiple people using places are being managed to prevent disasters by the special law for safety supervising of multiple people using spots. The KESCO is managing the places through intensification of inspection. Perfect safety is not insured by frequencyinspections because disaster symptoms in electrical facilities include invisible faults. Especially, entertainment spots, such as entertainment bars and karaoke bars, are different from other multiple people using places, and active reaction to prevent electrical fires is impossible. Therefore, special protection plans to prevent electrical disasters are needed. In this paper, equipments of entertainment spots were researched, and their electrical characteristics were analyzed. The function for detecting aloose terminal, which is one of the main causes of electrical fires, was developed.

• 소음진동
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• 제11권2호
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• pp.315-322
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• 2001

This paper is focused on the frictionally induced thermoelastic instability (TEI) in automotive disk brakes. This instability leads to the formation of localized high temperature contact regions known as hot spots. This article investigates the themoelastic instability in automotive disk brake systems consisting of a finite thickness layer (disk) and two half-planes (pads) using a perturbation method. The antisymmetric mode involves hot spots located alternately on two sides of the disk. As a result the circumferentially periodic hot spots produce rotor surface distortion and Induce low frequency vibration. Also the effects of system parameters on the critical speed for TEI are investigated.

• 한국응용과학기술학회지
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• 제14권1호
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• pp.103-107
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• 1997

This study was carried out to separate and identify the antioxidative substances in persimmon leaves. The antioxidative substances in persimmon leaves were extracted by methanol. The extract was fractionnated by SEP-PAK cartridge colum. From these results five fractions(F-I${\sim}$V) were obtained. Antioxidative activity of each fractions was examined by the DPPH methord. The F-II, III and IV showed antioxidative activity and among them F-II and F-III showed the strongest. Five frations were separated by TLC using ethylacetate : chloroform : formic acid : $H_2O$(8 : 1 : 1 : 1 v /v) as the solvent. From these results were obtained spots of Rf 0.71, 0.35 and 0.25. This spots were scraped from the plate and extracted by methanol. The extracts thuse obtained were used for examination of identify by TLC, UV /VIS-spectrophotometer and HPLC. Among them spot of Rf 0.71 were demonstrated to catechin and the spots of Rf 0.35 and 0.25 was suggested to polyphenol substances.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 추계학술대회
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• pp.1080-1085
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• 2003

The flow of electrical current through a microscopic actual contact spot between two conductors is influenced by the flow through adjacent contact spots. A smoothed version of this interaction effect is developed and used to predict the contact resistance when the statistical size and spatial distribution of contact spots is known. To illustrate the use of the method, an idealized fractal rough surface is defined using the random midpoint displacement algorithm and the size distribution of contact spots is assumed to be given by the intersection of this surface with a constant height plane. With these assumptions, it is shown that including finer scale detail in the fractal surface, equivalent to reducing the sampling length in the measurement of the surface, causes the predicted resistance to approach the perfect contact limit.

• Journal of Crop Science and Biotechnology
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• 제11권4호
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• pp.227-232
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• 2008

Drought stress is one of the major abiotic stresses in agriculture worldwide. We report here a proteomic approach to investigate the impact of post-fertilization drought on grain quality in rice seed endosperm (Oryza sativa cv. IR-64). Plants were stressed for 4 days at 3 days before heading. Total proteins of endosperm were extracted and separated by two-dimensional gel electrophoresis. Not many protein spots showed differential accumulation in drought-stressed samples. More than 400 protein spots were reproducibly detected, including three that were up-regulated and five down-regulated. Mass spectrometry analysis and database searching helped us to identify six spots representing different proteins. Functionally, the identified proteins were related to protein synthesis and carbohydrate metabolism, such as Granule-Bound Starch Synthase (GBSS, Wx protein), which is thought to play a very important role in starch biosynthesis and quality, a very crucial factor in determining rice grain quality.