• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.233-239
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• 2021

Cyanobacteriochromes (CBCRs) are phytochrome-related photoreceptor proteins in cyanobacteria and cover a wide spectral range from ultraviolet to far-red. A single GAF domain that they contain can bind bilin(s) autocatalytically via heterologous recombination and then fluoresce, with potential applications as biomarkers and biosensors. Here, we report that a novel red/green CBCR GAF domain, SPI1085g2 from Spirulina subsalsa, covalently binds both phycocyanobilin (PCB) and phycoerythrobilin (PEB). The PCB-binding GAF domain exhibited canonical red/green photoconversion with weak fluorescence emission. However, the PEB-binding GAF domain, SPI1085g2-PEB, exhibited an intense orange fluorescence (λabs.max = 520 nm, λfluor.max = 555 nm), with a fluorescence quantum yield close to 1.0. The fluorescence of SPI1085g2-PEB was selectively and instantaneously quenched by copper ions in a concentration-dependent manner and exhibited reversibility upon treatment with the metal chelator EDTA. This study identified a novel PEB-binding cyanobacteriochrome-based fluorescent protein with the highest quantum yield reported to date and suggests its potential as a biosensor for the rapid detection of copper ions.

• 한국지하수토양환경학회지:지하수토양환경
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• 제25권4호
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• pp.14-27
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• 2020

The drought has occurred from the past, and has caused a lot of damage. It is important to analyze the past droughts and predict them in the future. In this study, the temperature and precipitation of the past and the future from climate change RCP 4.5 and 8.5 scenarios were analyzed for Seosan and Boryeong in the western region of Chungnam Province, which is considered as a drought-prone area on the Korean Peninsula. Comparing Standardized Precipitation Index (SPI) and Effective Drought Index (EDI) based on the past droughts, EDI was verified to be more suitable for the drought assessment. According to RCP 4.5, the frequency and intensity of droughts in the early future (2021~2060) were expected to increase and to be stronger. Particularly, severe droughts were predicted for a long time from 2022 to 2026, and from 2032 to 2039. Droughts were expected to decrease in the late future (2061~2100). From RCP 8.5, drought occurrences were predicted to increase, but the intensity of the droughts were expected to decrease in the future. As a result of evaluation of the frequencies of droughts by seasons, the region would be most affected by fall drought in the early future and by spring drought in the late future according to RCP 4.5. In the case of RCP 8.5, the seasonal effects were not clearly distinguished. These results suggest that droughts in the future do not have any tendency, but continue to occurr as in the past. Therefore, the measures and efforts to secure water resources and reinforcement of water supply facilities should be prepared to cope with droughts.

• Preventive Nutrition and Food Science
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• 제17권3호
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• pp.223-227
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• 2012

This study examined the effects of corn gluten (CG), wheat gluten (WG), and soybean protein isolate (SPI), as well as their hydrolysates, on weight reduction in rats fed a high-fat diet. Eight-month-old male Sprague-Dawley rats (n=70) were fed a high-fat diet (40% of the calories were fat) for 4 weeks. Rats were then randomly divided into seven groups and were fed isocaloric diets with different protein sources for 8 weeks. The protein sources were casein (control group), intact CG (CG group), CG hydrolysate (CGH group), intact WG (WG group), WG hydrolysate (WGH group), intact SPI (SPI group), and SPI hydrolysate (SPIH group). Body weight gain, adipose tissue weights, lipid profiles in plasma and liver; and hepatic activities of carnitine palmitoyl transferase, fatty acid synthase (FAS), malic enzyme, and glucose-6-phosphate dehydrogenase were assessed. The CGH group showed significant weight reduction compared with the other groups. Epididymal fat pad and plasma triglycerides in the CGH group were the lowest and were significantly different than those in the control group. FAS activity in the CGH group was significantly lower than that in the other groups. In conclusion, the CGH diet of these experimental animals demonstrated a weight-reducing effect by lowering the adipose tissue weight and by affecting the activities of hepatic lipogenic enzymes.

• Journal of Microbiology and Biotechnology
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• 제27권11호
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• pp.1983-1993
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• 2017

Salmonella enterica subsp. enterica serovar Typhimurium, one of the most common foodborne pathogens, is transmitted mainly through contaminated food derived from infected animals. In this study, S. Typhimurium ST1120, an isolate from pig feces in Korea, was subjected to whole-genome analysis to understand its genomic features associated with virulence. The genome of ST1120 was found to have a circular chromosome of 4,855,001 bp (GC content 52.2%) and a plasmid of 6,863 bp (GC content 46.0%). This chromosome was predicted to have 4,558 open reading frames (ORFs), 17 pseudogenes, 22 rRNA genes, and 86 tRNA genes. Its plasmid was predicted to have three ORFs. Comparative genome analysis revealed that ST1120 was phylogenetically close to S. Typhimurium U288, a critical isolate in piggery farms and food chains in Europe. In silico functional analysis predicted that the ST1120 genome harbored multiple genes associated with virulence and stress resistance, including Salmonella pathogenicity islands (SPIs containing SPI-1 to SPI-5, SPI-13, and SPI-14), C63PI locus, ST104 prophage locus, and various antibiotic resistance genes. In accordance with these analysis results, ST1120 showed competence in invasion and survival abilities when it was added to host cells. It also exhibited robust resistance against antibiotics in comparison with other S. Typhimurium strains. This is the first report of the complete genome sequence of S. Typhimurium isolated from swine in Korea. Comparative genome analysis between ST1120 and other Salmonella strains would provide fruitful information toward understanding Salmonella host specificity and developing control measures against S. Typhimurium infection.

• 한국식품영양과학회지
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• 제32권1호
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• pp.75-81
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• 2003

전지활성 생대두미세분말(micronized full-fat soyflour, MFS)로부터 제조된 두유는 의가소성 의 비뉴턴성 유체로서, 고형분 증가와 응고제의 첨가 및 가열에 따라 점도가 증가하였다. MFS용액은 열처리에 따른 점도가 급격하게 증가되는 시점의 온도는 응고제의 첨가 및 단백질의 함량이 증가함에따라 낮아졌다 충진형 전두부제조에 적합한 응고제는 가열된 MFS용액에 응고제 첨가 후 MFS용액의 점도를 완만하게 변화시키는 GDL, MgSO$_4$가 적 합한 것으로 나타났으며 전두부용 응고제는 0.1% salt, 0.1% GDL, 0.2% MgSO$_4$ㆍ7$H_2O$로 조제되었다. 고형분과 물의 비율이 1 : 6.0인 MFS60용액을 121$^{\circ}C$에서 3분간 가열하여 응고제를 첨가하여 제조된 전두부는 견고성과 깨짐성이 양호한 조직감을 나타내었으며, 여기에 SPI를 첨가하여 총단백질이 7.1%인 MFSS60용액으로 제조된 전두부의 경도와 깨짐성(brittleness)은 각각 120 g, 176 g으로 조직감이 개선되었다.

• 한국식품영양과학회지
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• 제35권9호
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• pp.1251-1255
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• 2006

대두단백 필름을 코팅한 라이너지의 효과를 알아보기 위하여 대두단백 $2\sim8%$의 농도 범위에서 필름을 제조하였다. 필름 제조시 친수성 소재인 대두단백의 불용화를 위하여 모든 필름은 formaldehyde로 포화된 데시케이터에서 2시간 동안 흡착하여 사용하였다. 필름의 연신강도, 연신율, 투습도 및 수분용해도를 측정한 뒤 최적 농도를 5%로 판단하였다. 필름의 제조적성을 위해 첨가된 가소제 glycerol은 대두단백 대비 40% 농도에서 필름제조에 가장 적합하였다. 앞의 조건을 이용하여 제조된 용액을 라이너지에 코팅하여 그 물성과 수분저항성을 측정하였다. Formaldehyde 처리된 대두단백 코팅 라이너지는 미처리 라이너지에 비하여 연신강도는 15 에서 21 MPa로 증가하였고, 수분용해도와 투습계수는 1.17%와 $2.06ng{\cdot}m/m^2{\cdot}s{\cdot}Pa$로 감소하여 물리적 성질과 수분저항성 모두 증진된 것을 알 수 있었다.

• 한국물환경학회지
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• 제37권5호
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• pp.369-380
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• 2021

In this work, we analyzed the effects of drought on the water temperature (WT) of Nakdong river basin major river sections using Standardized Precipitation Index (SPI) and WT data. The analysis was carried out on a seasonal basis. After calculating the optimal time scale of the SPI through the correlation between the SPI and WT data, we used the copula theory to model the joint probability distribution between the WT and SPI on the optimal time scale. During spring and fall, the possibility of environmental drought caused by high WT increased in most of the river sections. Notably, in summer, the possibility of environmental drought caused by high WT increased in all river sections. On the other hand, in winter, the possibility of environmental drought caused by low WT increased in most river sections. From the risk map, which quantified the sensitivity of WT to the risk of environmental drought, the river sections Nakbon C, Namgang E, and Nakbon K showed increased stress in the water ecosystem due to high WT when drought occurred in summer. When drought occurred in winter, an increased water ecosystem stress caused by falling WT was observed in the river sections Gilan A, Yongjeon A, Nakbon F, Hwanggang B, Nakbon I, Nakbon J, Nakbon K, Nakbon L, and Nakbon M. The methodology developed in this study will be used in the future to quantify the effects of drought on water quality as well as WT.

• 한국정보통신학회논문지
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• 제24권10호
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• pp.1361-1368
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• 2020

인원을 계수하기 위한 시스템은 기존에 다양한 방법으로 이루어지고 있다. 기존 시스템의 계수방식으로는 적외선 센서, 레이저, 카메라 등이 있는데 이러한 기존 시스템의 경우에는 천장, 벽의 측면 등과 같이 공간에 대한 제약이 발생한다. 본 논문에서는 다수의 압전소자가 포함된 압전매트를 이용하여 보행자의 발걸음을 검출하고 압전매트에서 수집된 데이터를 활용하여 동시에 통과하는 보행 인원을 계수하는 방법을 제시한다. 보행자가 압전매트 위를 지나갈 경우, 수집한 센서 데이터를 SPI 통신을 이용하여 종합하고 TCP/IP 통신을 이용해 PC서버로 전송하는 방식의 통신시스템을 이용하였다. 성능분석 결과, 약 600개가량의 발걸음 데이터에서 99% 정도의 정확도로 인식하는 결과를 얻었다. 이를 통해 다른 인원 계수 시스템의 단점을 극복하고자 한다.

• 한국포장학회지
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• 제15권3호
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• pp.99-104
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• 2009

Soy protein isolate (SPI)-based nanocomposite films with three different types of nanoclays, such as Cloisite $Na^+$, Cloisite 20A, and Cloisite 30B, were prepared using a solution casting method, and their optical, tensile, and water vapor barrier properties were determined to investigate the effect of nano-clay type on film properties. Among the tested nanoclays, Cloisite $Na^+$, a hydrophilic montmorillonite (MMT), exhibited the highest transparency with least opaqueness, the highest tensile strength, and the highest water vapor barrier properties, indicating Cloisite $Na^+$ is the most compatible with SPI polymer matrix to form nanocomposite films. The film properties of SPI/Cloisite $Na^+$ nanocomposite films were strongly dependent on the concentration of the clay. Film properties such as optical, tensile, and water vapor barrier properties improved significantly (p<0.05) as the concentration of clay increased. However, the effectiveness of addition of the clay reduced above a certain level (i.e., 5wt%), indicating that there is an optimum amount of clay addition to exploit the full advantage of nanocmposite films.

• 한국습지학회지
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• 제21권spc호
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• pp.80-89
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• 2019

본 논문의 목적은 금강유역을 대상으로 Standardized Precipitation Index (SPI) 기상학적 가뭄지수, 농업용 저수지 가뭄지수(Reservoir Drought Index, RDI)간의 관계를 통해 기상학적 가뭄이 농업에 미치는 상관성을 분석하는데 있다. 2014년부터 2016년까지의 강수량, 농업용 저수율 자료를 수집하여 가뭄지수를 산정하였으며, 기상학적 가뭄과 농업가뭄간의 상관성을 평가하기 위해 Pearson 상관계수와 Receiver Operation Characteristic (ROC) 분석을 실시하였다. 상관분석결과 SPI-6와 RDI의 Pearson 및 ROC 적중룰이 각각 0.606, 0.722으로 가장 높게 분석되었고 가뭄의 공간적 발생패턴을 분석하기 위해 공간분포된 SPI-6와 RDI를 중첩한 결과 2015년 8월부터 2015년 10월의 심한 가뭄 발생 시 미호천 상류 유역과 논산천 유역에서 중복적으로 가뭄의 심도의 차이가 발생하는 것을 확인하였다. 저수지 가뭄이 발생한 지역에 대한 저수지의 제원을 이용하여 분석을 수행한 결과, RDI 가뭄이 크게 나타난 지역은 유역배율이 작은 저수지들이 많이 모여 있는 지역일수록 극심한 가뭄을 겪는 것으로 분석되어, 유역배율에 따라 저수지의 농업가뭄 대응능력에 차이가 있음을 확인하였다.