• Journal of the Korean Society for Geothermal and Hydrothermal Energy
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• v.11 no.4
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• pp.7-13
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• 2015

The efficiency, reliability and performance of any heat pump unit can only be ascertained after it has been tested and rated. For this reason, specific facilities, equiped with testing plants are built. Heat pump calorimeter is the facilities used by most of these testing facilities in their rating and certification process. The ultimate function of calorimeters is to, control and maintain the constant standard test conditions (indoor/outdoor entering temperatures and flow rate etc) during testing period. In this study, the test standards of heat pump unit and the structure of the calorimeter are surveyed. In addition, this study analyzes the total energy consumption of a water to water heat pump calorimeter. Heat pump calorimeter consumed much energy to excute the heat pump tests. The energy consumption of the calorimeter was higher than the heat pump unit, and it was increased as the heat pump unit capacity decreased.

• ALGAE
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• v.23 no.4
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• pp.289-294
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• 2008

To estimate seaweed biomass or standing crop, a nondestructive sampling can be beneficial because of not much destroying living plants and saving time in field works. We suggest a methodological procedure to estimate seaweed biomass per unit area in marine benthic habitats by using species-specific regression equations. Percent cover data are required from the field samplings for most species to convert them to weight data. However, for tall macroalgae such as kelps we need density data and their size (e.g., size class for subtidal kelps) of individuals. We propose that the field sampling should be done with 5 replicates of 50 cm x 50 cm quadrat at three zones of intertidals (upper, middle, lower) and three depth points (1, 5, 10 m) in subtidals. To obtain a reliable regression equation for a species, a substantial number of replicate is necessary from destructive samplings. The regression equation of a species can be further specified by different locality and different season, especially for the species with variable morphology temporally and spatially. Example estimation carried out in Onpyung, Jeju Island, Korea is provided to compare estimated values with real weight data.

• Mycobiology
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• v.45 no.3
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• pp.139-149
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• 2017

The genus Hyaloperonospora (Peronosporaceae; Oomycota) is an obligate biotrophic group that causes downy mildew disease on the Brassicaceae and allied families of Brassicales, including many economically relevant crops, such as broccoli, cabbage, radish, rape, and wasabi. To investigate the diversity of Hyaloperonospora species in northeast Asia, we performed a morphological analysis for the dried herbarium specimens collected in Korea, along with molecular phylogenetic inferences based on internal transcribed spacer rDNA and cox2 mtDNA sequences. It was confirmed that 14 species of Hyaloperonospora exist in Korea. Of these, three species, previously classified under the genus Peronospora, were combined to Hyaloperonospora: H. arabidis-glabrae comb. nov. (ex Arabis glabra), H. nasturtii-montani comb. nov. (ex Rorippa indica), and H. nasturtii-palustris comb. nov. (ex Rorippa palustris). In addition, finding two potentially new species specific to northeast Asian plants is noteworthy in support of the view that the species abundance of Hyaloperonospora has been underestimated hitherto.

• Nuclear Engineering and Technology
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• v.46 no.5
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• pp.569-580
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• 2014

Seismic isolation is a viable strategy for protecting safety-related nuclear structures from the effects of moderate to severe earthquake shaking. Although seismic isolation has been deployed in nuclear structures in France and South Africa, it has not seen widespread use because of limited new build nuclear construction in the past 30 years and a lack of guidelines, codes and standards for the analysis, design and construction of isolation systems specific to nuclear structures. The funding by the United States Nuclear Regulatory Commission of a research project to the Lawrence Berkeley National Laboratory and MCEER/University at Buffalo facilitated the writing of a soon-to-be-published NUREG on seismic isolation. Funding of MCEER by the National Science Foundation led to research products that provide the technical basis for a new section in ASCE Standard 4 on the seismic isolation of safety-related nuclear facilities. The performance expectations identified in the NUREG and ASCE 4 for seismic isolation systems, and superstructures and substructures are described in the paper. Robust numerical models capable of capturing isolator behaviors under extreme loadings, which have been verified and validated following ASME protocols, and implemented in the open source code OpenSees, are introduced.

• Proceedings of the Botanical Society of Korea Conference
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• 1998.07a
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• pp.61-70
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• 1998

Genome and chromosome analyses in plants using fluorescence in situ hybridization (FISH) and immuno-staining (IMS) methods are reviewed by presenting the recent results obtained by the Chromosome Link, a group of chromosome and genome researchers. FISH is now effective to detect unique nucleotide sequences with 153 bp on the extended DNA fibers. Genomic in situ hybridization (GISH) also allows painting plant chromosomes of different genomes. GISH is quite effective to detect the genomic differentiation in the individual chromosomes within a nucleus. Three dimensional (3D) analyses are now available by confocal microscopy and a deconvolution system. These techniques are invaluable to visualize both the structural and functional dynamics within a nucleus. 3D-FISH revealed the spatial differentiation of different genomees within a nucleus. 3D-FISH also proved structural partition of centromeric and telomeric domains within a barely nucleus. The dynamic acetylation of histone H4 at the specific regions of a genome during a cell cycle is also analyzed using 3D-IMS. It is anticipated that these methods will provide us powerful tools to understand the structural and functional significance of plant chromosomes and genomes.

• Proceedings of the Botanical Society of Korea Conference
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• 1996.07a
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• pp.26-38
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• 1996

To investigate the regulation of plant histone H2A gene expression, we isolated two H2A genes (TH254 and TH274) from wheat, which encode different types of variants. Both genes had an intron in the coding region. In the promoters, some characteristics sequences, such as Oct and Nona motifs, which are conserved among plant histone genes were also found, and they were located in a short region (about 120 bp) upstream from the putative TATA box. Analyses of promoter activity with H2A-GUS fusion genes in the transient system using tobacco protoplasts revealed novel types of positive cis-acting sequences in the TH254 promoter: a direct repeat of a 13-bp sequence (AGTTACATTATTG) and a stretch composed of an AT-rich sequence (ATATAGAAAATTAAAA) and a G-box (CACGTG). A quantitative S1 assay of the mRNA amounts from the TH254/GUS and TH274/GUG chimeric genes in stably transformed and cell cycle-synchronized tobacco cell lines showed that the promoters of both genes contained at least one cis-acting element responsible for S phase-specific expression. Histochemical analysis of transgenic tobacco plants carrying the chimeric genes showed that the promoters of the two H2A genes were both active in developing seedlings and flower organs but regulated in different manner.

• Molecules and Cells
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• v.19 no.1
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• pp.81-87
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• 2005

Phytochelatins play an important role in heavy metal detoxification in plants as well as in other organisms. The Arabidopsis thaliana mutant cad1-3 does not produce detectable levels of phytochelatins in response to cadmium stress. The hypersensitivity of cad1-3 to cadmium stress is attributed to a mutation in the phytochelatin synthase 1 (AtPCS1) gene. However, A. thaliana also contains a functional phytochelatin synthase 2 (AtPCS2). In this study, we investigated why the cad1-3 mutant is hypersensitive to cadmium stress despite the presence of AtPCS2. Northern and Western blot analyses showed that expression of AtPCS2 is weak compared to AtPCS1 in both roots and shoots of transgenic Arabidopsis. The lower level of AtPCS2 expression was confirmed by RT-PCR analysis of wild type Arabidopsis. Moreover, no tissue-specific expression of AtPCS2 was observed. Even when AtPCS2 was under the control of the AtPCS1 promoter or of the cauliflower mosaic virus 35S promoter (CaMV 35S) it was not capable of fully complementing the cad1-3 mutant for cadmium resistance.

• Journal of Korean Society for Atmospheric Environment
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• v.17 no.E2
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• pp.61-70
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• 2001

In recent years there have been growing interests in the potential environmental effects of global climate change. Of specific interests is the role that climate change may play in altering natural volatile organic compound.(NVOC) emissions from trees and the subsequent impact of this perturbation on air quality and ozone formation. A novel vegetation enclosure chamber method was designed and constructed of Tedlar in order to estimate more accurate and precise NVOC emission rates of either small whole plants or the branches of large trees. The enclosure chamber was initially tested in the laboratory and also successfully evaluated in the field. Overall precision for this enclosure was estimated as RSD<10%(n=9). The overall errors associated with the enclosure method in a laboratory system might be relatively small (say<$\pm$15%); however, they might be rather large(say$\pm$40%) in a field-based system. Two consecutive samples were collected on each sampling day from the two pine species during the test period. Slash pine studies showed that the absolute percentage difference between the first and second samples varied from 0.33 to 29%. The percent differences between consecutive emission for loblolly pines varied from 0.74 to 24.2%.

• Journal of Applied Biological Chemistry
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• v.42 no.4
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• pp.169-174
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• 1999

Arabidopsis AHL gene contains 4 exons encoding a putative protein highly homologous to the yeast salt-sensitive enzyme HAL2, a 3'(2'),5'-bisphosphate nucleotidase involving in reductive sulfate assimilation. AHL cDNA complemented yeast met22 (hal2) mutant. AHL fusion protein expressed in E. coli exhibited $Mg^{2+}$-dependent, 3'-phosphoadenosine 5'-phosphate (PAP)-specific phosphatase activity. $Li^+,\;Na^+,\;K^+$ and $Ca^{2+}$ ions inhibit the enzyme activity by competing with $Mg^{2+}$ for the active site of the enzyme. The enzyme activity was also sensitive to ${\mu}M$ concentrations of toxic heavy metal ions such as $Cd^{2+},\;Cu^{2+}$ and $Zn^{2+}$, but was not recovered by addition of more $Mg^{2+}$ ions, suggesting that these ions inactivate the enzyme with a mechanism other than competition with $Mg^{2+}$ ions. Inhibition of the AHL enzyme activity may result in accumulation of PAP, which is highly toxic to the cell. Thus, the AHL enzyme could be one of the intial targets of heavy metal toxicity in plants.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2000.10a
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• pp.477-483
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• 2000

Fly ash is the unburned residue resulting from the combustion of coal in utility and industrial boilers such as thermal power plants. Annually about 5 million tons of fly ash is being produced in korea. Less than 25 percent of total volume of fly ash is currently being used effectively for some ways. In the future, the volume of fly ash discharge from thermal power stations will be increasing more and more, and the development of the utilization of high volume fly ash is required. Fly ash has a lower compacted density and specific gravity than coarse grained natural aggregates but equivalent strength properties indicating that the fly ash could be used as a structural fill materials. So, clay-fly ash mixtures can be used as a fill material in the construction of embankments. Laboratory tests have been carried out to determine the physical, chemical, and geotechnical characteristics of the clay and fly ash. The fly ash is mixed with the clay in different proportions and the geotechnical characteristics of the mixtures have been studied also. In this study describes the results of the experimental study. The implications of the use of clay and clay-fly ash mixtures on the stability of embankments are discussed.