• 한국직업건강간호학회지
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• 제4권호
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• pp.48-57
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• 1995

The purpose of this study was to assess the performance of the role and function of some industrial nurses and to characterize the factors affecting the performance of their activities. Thus the results could be used to suggest the direction in the performance of industrials nurses' activities effectively. During a period from January 10 to March 31, 1994, the data were collected from 87 industrial nurses, who were working as health managers in the plants, in Ulsan city and the vicinity in Kyungnam province, using a structured questionnaire. The results were as follows : 1. The general characteristics of industrial nurses in this study were 82.8% being 30 years old or less, 60.9%, being not married, and 93.1% having eduction levels above junior college. 2. With respect to general work conditions, 94.3% were working in a separate room provided for health care division, 40.2% working under the safety and health department, and 98.9% working as common-level staffs. And 60.9% were working less than 44 hours a week, 70.1% had work experiences less than 5 years, and 50.6% had annual incomes ranging 10 to 14 million wons. 3. As work conditions related to health care activities, 49.4% performed the activities not related to health care as always or occasionally, and 87.4% answered that occupational physicians were appointed in their plant and among them, however, only 6.9% worked on full-time basis and 52.8% perform little activities as occupational physicians. For a decision related to health care activity, 69.0% discussed the problems with the supervisors, and 19.5% made decisions by themselves. 4. As for attitude and perception to their activities as health managers, 66.7% moderately recognized the importance of health manager in the workplace, with 63.2% being satisfied their wages and treatment from the company, 57.5% being satisfied with their job positions and 51.7% having positive attitudes as being health managers. 5. The degree of performance at least in one of health related activities were very high in activities such as general medical care(100%), general health examination(98.0%) and specific health examination(100%), and relatively high in health education(72%), new employee health examination(60.9%), document handling(79.3%) and activity for work environment(70.1%). However, the performance rate was very low in preparing protective equipment (20.8%). 6. The levels of activities related to health care were significantly high when making decisions by themselves, when occupational physicians not being full-time, and when satisfying their job positions, and, on the other hand, significantly decreased as work hours increased. 7. In addition to some kinds of periodic education asked by all of the nurses, 89.7% wanted a specialized licensing system for industrial nurse, and 97.4% wanted to apply for the license test. As a conclusion, it is suggested that industrial nurses should be given more authority and placed in more self-controlled system to perform health care and other activities more efficiently, and the role and function of the occupational physician should be clearly distinguished from that of the industrial nurse as a health manager to avoid an unnecessary overlapping.

• Journal of Plant Biology
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• 제38권1호
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• pp.63-72
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• 1995

온대지방의 수 종 목본식물에서 생육초기 동일 줄기로보터 동시 개엽하는 잎들의 생장과 크기 및 형태의 차이를 조사하고 이를 나타내는 방법을 시도하였다. 조사된 12종에서 조사기간 중에 동일 줄기로부터개엽한 총엽수는 3~7개이며, 이중 동시 개엽한 엽수는 2~6개이었다. 동시 개엽한 잎의 엽면적 생장은 조사시기의 마지막인 6월 10일에 거의 완료되었지만 잎의 무게는 계속 증가하였다. 그리고 동시 개엽한 잎들의 비엽면적의 최대치는 유사한 시기에 나타났다. 굴참나무를 제외한 11종에서 엽순에 따라 엽신의 길이와 폭의 차이에 유의성이 있었고, 진달래를 제외한 10종에서 전후엽 사이에서 0.1% 혹은 5% 수준에서 유의적인 차가 존재하였다. 엽순에 따른 잎의 이형성지수는 엽신의 길이와 폭으로부터 산출한 값인 엽신의 $길이{\times}폭$과 길이/폭에서 각각 평균 편차를 이용하여 구하였다. 이 결과 잎의 이형성 지수는 12종 중 개서나무, 쪽동백나무, 느릅나무, 짝짜래나무 및 노린재나무 등이 큰 것으로, 팥배나무와 피나무가 작은 것으로 나타났다. 이 결과는 실제 잎의 형태적 차이를 유사하게 표현하였다. 그리고 잎의 이형성 지수는 그 종의 생장형태나 잎의 다른 특징과 관계가 없었고, 환경에 대한 적응의 한 형태로 사료되었다.

• Molecules and Cells
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• 제25권3호
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• pp.407-416
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• 2008

Thirteen near-isogenic lines (NILs) of japonica rice were developed via a backcross method using the recurrent parent Chucheong, which is of good eating quality but is susceptible to Magnaporthe grisea, and three blast resistant japonica donors, Seolak, Daeseong and Bongkwang. The agro-morphological traits of these NILs, such as heading date, culm length, and panicle length, were similar to those of Chucheong. In a genome-wide scan using 158 SSR markers, chromosome segments of Chucheong were identified in most polymorphic regions of the 13 NIL plants, and only a few chromosome segments were found to have been substituted by donor alleles. The genetic similarities of the 13 NILs to the recurrent parent Chucheong averaged 0.961, with a range of 0.932-0.984. Analysis of 13 major blast resistance (R) genes in these lines using specific DNA markers showed that each NIL appeared to contain some combination of the four R genes, Pib, Pii, Pik-m and Pita-2, with the first three genes being present in each line. Screening of nine M. grisea isolates revealed that one NIL M7 was resistant to all nine isolates; the remaining NILs were each resistant to between three and seven isolates, except for NIL M106, which was resistant to only two isolates. In a blast nursery experiment, all the NILs proved to be more resistant than Chucheong. These newly developed NILs have potential as commercial rice varieties because of their increased resistance to M. grisea combined with the desirable agronomic traits of Chucheong. They also provide material for studying the genetic basis of blast resistance.

• Applied Biological Chemistry
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• 제29권1호
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• pp.73-77
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• 1986

Auxin과 cytokinin 그리고 $GA_3$가 대두(Glycine max) 종자의 단백질 합성과 축적에 미치는 영향을 보기 위하여, 2,4-D와 BA 그리고 $GA_3$을 각각 $10^{-6},\;10^{-5},\;10^{-4}M$의 수용액으로 만들어 개화기에 식물체 전체에 살포 처리하고 개화 후 33일의 미숙종자와 77일의 완숙종자에 대한 단백질 전기영동 양상을 조사하였다. $GA_3$는 단백질 합성과 축적에 영향을 주지 않았으나 2,4-D와 BA는 일정농도에서 특정 단백질들의 축적에 영향을 주었다. 7S의 ${\alpha}$와 ${\alpha}'$ subunit, 11S의 acidic subunit 들이 2,4-D 혹은 BA처리에 의하여 나타나지 않았으며, 이 subunit들이 성숙종자에 없으나 미숙종자에는 있는 점으로 보아, 2,4-D나 BA는 이들의 함성을 저해하는 것이 아니고 축적과정의 어느 단계에서 이 subunit들의 분해 혹은 전환을 촉진하는 것으로 생각되었다. 2,4-D와 BA에 의해 위의 단백질이 성숙종자에 축적되지 않는 것은 어떤 단백질분해효소(metalloendopeptidase?)의 작용과 관련이 있는 것으로 보이며, 2,4-D나 BA는 성숙후기에 이 효소의 gene expression을 촉진하거나 활성을 증진시키는 것으로 생각되었다.

• 한국보건간호학회지
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• 제2권2호
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• pp.62-80
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• 1988

The main purpose of this study was to find out the actual status of safety and heal th education activities in the manufacturing industries through survey of 136 plants in Seoul City and Gyunggi- Province Area which employ nurses being charged in the safety and health care services to the employees. A questionaire was mailed to the employees on the Mar. 2, 1987. Total 634 responds from 87 industries were collected by Apr. 20, 1987. Among the total, 618 responds from 80 industries were included in the analysis. The major findings obtainded from this study are summarized as follows; 1. Safety and health education activities in each industry: 1) The $67.6\%$ of safety directors surveyed were performing the education to the employees. And in case of medical directors, it was $18.8\%$ of them. 2) Periodically, annual safety' and health education programs were being drawn up in the $65.0\%$ of the industries (52 companies). And the $60.6\%$ of the planners were safety directors of safety staffs in charge. 3) It was only $27.5\%$ of the companies surveyed in which the safety and health education were performed more than an hour every month. In the $22.5\%$ of the companies, neither safety programs nor health education activities were performed. 4) In the $47.5\%$ of them, safety and health educations were performed in cooperation with related agencies such as health center. 2. The rate of employees participated in safety and health educations; 1) The received rates by subjects of the educations when labors were newly employed to their companies were as follows; education regarding danger and profer handling method of machinery and appliances: $64.2\%$, education regarding noxiousness and handling method of raw materials: $42.2\%$, etc. 2) The $63.6\%$ of the labors received educations on safety and health when they changed their work places. 3) The $74.8\%$ of the labors received specific safety and health educations. 4) The general safety and health educations were received by the $47.2\%$ of management and clerical personnel and $50.0\%$ of labors pre and post physical examination. 3. The main reasons of inactive performance of the educations were as follows; lack of knowledge and inexperience of the occupational safety and health staffs, lack of cooperations between themselves and low need of workers for safety and health education, etc. 4. The preferable subjects of educations for workers; (1) pre and post education of physical examination, (2) education regarding the prevention of accidents. (3) general health care, sex education and family planning, etc. As a result of this study, we can conclude that the safety and health education work in industries as the subject of this study is on the incipient stage. Appropriate measures are to be taken for the activation of safety and health education work such as; continuous public relations, financial and technical supports of the government, training of professional/occupational safety and health staffs, efforts of workers to receive the education and collaborations of the employers.

• Journal of Ginseng Research
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• 제41권4호
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• pp.469-476
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• 2017

Background: Panax ginseng Meyer (Asian ginseng) has a large nuclear genome size of > 3.5 Gbp in haploid genome equivalent of 24 chromosomes. Tandem repeats (TRs) occupy significant portions of the genome in many plants and are often found in specific genomic loci, making them a valuable molecular cytogenetic tool in discriminating chromosomes. In an effort to understand the P. ginseng genome structure, we characterized an ultrahigh copy 167-bp TR (Pg167TR) and explored its chromosomal distribution as well as its utility for chromosome identification. Methods: Polymerase chain reaction amplicons of Pg167TR were labeled, along with 5S and 45S rDNA amplicons, using a direct nick-translation method. Direct fluorescence in situ hybridization (FISH) was used to analyze the chromosomal distribution of Pg167TR. Results: Recently, we reported a method of karyotyping the 24 chromosome pairs of P. ginseng using rDNA and DAPI (4',6-diamidino-2-phenylindole) bands. Here, a unique distribution of Pg167TR in all 24 P. ginseng chromosomes was observed, allowing easy identification of individual homologous chromosomes. Additionally, direct labeling of 5S and 45S rDNA probes allowed the identification of two additional 5S rDNA loci not previously reported, enabling the refinement of the P. ginseng karyotype. Conclusion: Identification of individual P. ginseng chromosomes was achieved using Pg167TR-FISH. Chromosome identification is important in understanding the P. ginseng genome structure, and our method will be useful for future integration of genetic linkage maps and genome scaffold anchoring. Additionally, it is a good tool for comparative studies with related species in efforts to understand the evolution of P. ginseng.

• 식물분류학회지
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• 제41권4호
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• pp.299-314
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• 2011

분자 마커의 선택은 집단유전학의 연구방법을 결정하는 중요한 고려사항으로, 현재까지 동식물의 집단유전학 연구에는 알로자임, RAPD, RFLP, AFLP, microsatellite, SNP, ISSR 등이 개발되어 주로 사용되고 있다. 이 중 microsatellite는 핵뿐만 아니라 엽록체, 미토콘드리아와 같은 세포소기관의 게놈상에 매우 풍부하게 존재하며, 핵에서 유래된 microsatellite는 높은 다형성을 보이는 공우성 마커로 집단 구조 및 유전적 다양성 연구에서 최근 선호된다. Microsatellite는 보통 1~6 bp의 짧은 서열이 반복된 것으로 각각의 유전자좌에 특화된 프라이머를 사용하여 증폭한다. Microsatellite는 PCR 반응으로 쉽게 유전자형을 분석할 수 있는 장점이 있으나, 종 특이적으로 개발되고 계통적으로 매우 가까운 근연종에게만 적용될 수 있는 단점이 있다. 따라서, 야생식물의 경우 microsatellite 개발에 필요한 게놈 정보가 부족하고 신규 개발비용이 많이 소요되어 적용이 쉽지 않았으나, 점차 개발비용이 낮아지고 있어, 야생식물을 대상으로 한 microsatellite 연구들이 증가하고 있는 추세이다. 따라서, 본 논문에서는 야생식물의 microsatellite를 이용한 분석 기초를 마련하고자 microsatellite 마커의 다양한 개발 및 분석 방법, 진화 모델 및 적용 분야에 대해 소개하고, 유전자형 결정시 잘못된 결론을 도출할 가능성이 높은 부분에 대한 사항들을 지적하여 야생식물의 microsatellite를 이용한 집단유전학적 분석에 도움을 주고자 하였다.

• 한국응용곤충학회지
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• 제14권4호
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• pp.214-214
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• 1975

This study was conducted to evaluated the nature of the variety Tongil(Suweon 213-1) in resistance to the striped rice borer, Chilo suppressalis Walker, comparing with those of Rexoro(susceptible check) and TKM-6(resistant check) selected at IRRI. 1. The striped rice borer moths much more preferred the variety Tongil for oviposition than the varieties Rexoro and TKM-6. The variety Tongil and more egg masses and number of eggs than the varieties Rexoro and TKM-6, while TKM-6 having more egg masses and more number of eggs than Rexoro. This reaction was consistent throughout the test regardless of the number of tillers per hill. 2. In laboratory, preference of larvae for feeding was studied with 5cm of stem pieces of the varieties. The results showed, in contrast to the case of ovipositional preference, that the striped rice borer larvae least preferred the stems of Tongil among the test varieties, while larvae much more preferred the stems of Rexoro than those of TKM-6. 3. The larval weights at 20 days later infested on the 40 day-old plants were the lowest on Tongil among the test varieties. On the variety Rexoro the larvae had heavier body weights(43.0mg), higher pupation(64.9%) and higher adult emergence(83.3%) than those on Tongil(larval weights 30.3mg, pupation 60%, adult emergence 60.7%) and TKM-6(larval weights 35.7mg, pupation 56.3%, adult emergence 51.9%). The pupal weights, however, were not consistent among the test varieties and/or sexes in comparison with the larval weights, pupation and adult emergence above mentioned. 4. Field experiments indicated that the incidence of dead hearts at 70 days after transplanting was relatively higher on the variety Tongil(11.1%) than those on Rexoro(8.9%) and TKM-6(8.4%), and the incidence of white heads at harvest was, in contrast to the dead hearts, lower on Tongil(9.8%) than those on Rexoro(27.4%) and TKM-6(13.9%). At harvest lower larval survival observed on Tongil (49 larvae/40 hills) than those on the susceptible variety Rexoro(104 larvae/40 hills) and on the resistant variety TKM-6(70 larvae/40 hills). The average larval weights collected from three test varieties at harvest were 80.5mg from Tongil, 83.7mg from TKM-6 and 99.6mg from Rexoro. 5. Increased nitrogen fertilizer application to the variety Tongil, the striped rice borer damage was increased. Also, preference of larvae for feeding significantly increased with the increase of nitrogen fertilizer application. 6. Any specific association between the plant characters and striped rice borer resistance could not be found. The variety Tongil even having large number of tillers, short plant height, large stem, broad leaf, etc, had still high preference of moths for oviposition, low preference of larvae for feeding, low damage, and relatively high antibiosis. 7. Resistance of the variety Tongil to the striped rice borers seemed to be associated with the low feeding preference and the relatively high antibiosis, not associated directly with the ovipositional preference.

• 대한기계학회논문집A
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• 제39권11호
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• pp.1169-1174
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• 2015

위험도 평가 기술은 주로 플랜트의 많은 운영설비 중 대형사고나 피해를 유발할 수 있는 위험설비를 선별하는 목적으로 개발되었다. 현재 발전소에 설치되어 있는 설비관리시스템에는 초기 버전의 위험도 평가 모듈이 설치되어 있으나 문진 위주로 구성되어 통계적 기법에 기반을 둔 고장 확률 평가보다는 평가자의 주관적인 판단에 따라 평가 결과가 좌우되었다. 또한 발전회사 서버에 기반하였기 때문에 평가자가 공간 및 시간의 제약을 받을 수 밖에 없고 현장 활용에 한계가 있었다. 본 논문에서는 문진 대신 해석적 분석을 이용한 기본 고장확률을 계산하고 현장 검사 결과 및 검사 효용도를 분석할 수 있는 독립형 위험도 평가 소프트웨어를 개발하였다. 독립형으로 현장 운용이 가능하며 최적 정비주기 예측 및 현장검사 결과에 대한 입출력 기능이 있기 때문에 정비에 직접 참여하는 현장 사용자뿐만 아니라 발전 설비의 수명연장 및 교체를 결정해야 하는 엔지니어에게 유용한 정보를 제공할 것이다.

• 한국환경생태학회지
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• 제23권4호
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• pp.333-345
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• 2009

우리나라 온대지방 산림의 극상수종으로 알려진 서어나무는 도시화가 심각한 서울의 경우 3개의 군집이 분포하고 있는 것으로 알려져 있다. 본 연구는 희귀하게 분포하는 서울지역 서어나무군집의 특성을 도출하고, 이를 바탕으로 서어나무군집의 관리대책을 마련하기 위한 기초자료 제공을 목적으로 수행되었다. 연구 대상지는 삼육대 지역, 도봉산 지역, 진관동 지역의 3개소이다. 서울 지역 서어나무군집에 대한군집구조 분석 및 토양특성, 수목 생장량 등을 조사 분석한 결과, 현재 남아있는 서어나무군집의 경우 토양 산성화 등으로 인해 생장 속도가 둔화되고 있는 것으로 분석되었다. 또한, 산림내 외래종의 이입에 따른 식생교란도 진행되고 있는 것으로 나타났다. 서울 지역에서 가장 넓은 서어나무군집이 분포하는 삼육대 지역의 경우 주변 참나무림에 참나무시들음병 피해가 발생하여 산림내부에 나지가 발생하고 있고 외래종의 이입속도가 더욱 빨라질 것으로 예상되고 있다. 또한 서어나무 자체도 참나무시들음병 매개곤충인 광릉긴나무좀의 피해를 받고 있어, 특별한 대책이 수립되지 않는 한, 향후 서어나무군집의 존립 자체가 불투명한 실정이다. 따라서 현재 남아있는 서어나무군집에 대한 정밀조사와 연차적인 모니터링 등과 같은 대책을 마련하여 서어나무군집 보전을 통한 서울지역 생물다양성 확보 방안의 추진이 필요하다.