• Journal of Microbiology and Biotechnology
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• 제15권4호
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• pp.772-779
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• 2005

Ammonia-oxidizing bacteria (AOB) were enriched by repeating fed-batch cultivations in an AOB-selective medium of activated sludges from a domestic wastewater treatment plant. Enriched culture showed strong capabilities of ammonia oxidation [0.810 mg $NH_4^+$-N/mg mixed liquor suspended solids (MLSS)$\cdot$day] as well as $NO_x^-$-N production (0.617 mg $NO_x^-$-N/ mg MLSS$\cdot$day). Degree of enrichment was examined through fluorescent in situ hybridization (FISH) analyses using an AOB-specific Cy3-labeled oligonucleotide probe (NSOl90) and terminal-restriction fragment length polymorphism (T-RFLP) analyses. FISH analyses confirmed that the fraction of AOB among 4',6-diamidino-2-phenylindole (DAPI)-stained cells increased from about less than $0.001\%$ to approximately $42\%$ after enrichment of AOB, and T-RFLP analyses showed that bacterial community became simpler as enrichment was continued. When the enriched culture of AOB was added (150 mg/l as dry suspended solid) to the normal activated sludge (3,000 mg/l as dry suspended solid), nitrification efficiencies were improved from 0.020 mg $NO_x^-$-N/mg MLSS$\cdot$day to 0.041 mg $NO_x^-$-N/mg MLSS$\cdot$day in a synthetic wastewater and also from 0.0007 mg $NO_x^-$-N/mg MLSS$\cdot$day to 0.0918 mg $NO_x^-$-N/mg MLSS$\cdot$day in a real domestic wastewater. Therefore, it is expected that this enrichment method could be used for improving efficiency of nitrification in wastewater treatment plants.

• 한국미생물·생명공학회지
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• 제44권4호
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• pp.493-496
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• 2016

Peach rosette mosaic virus (PRMV)는 1933년 복숭아에서 처음 보고되었으며, 복숭아, 자두, 블루베리, 민들레, 벚나무 등에 감염되는 식물바이러스이다. PRMV는 한국에서 보고된 적이 없으나, 식물검역에서 관리병(control viruses)으로 지정되어 있다. 이번 연구에서는 PRMV를 더욱 신속하고 특이적으로 진단하기 위하여 Loop-mediated isothermal amplification 분석법을 적용한 진단법을 개발하였다. LAMP 방법은 기존의 PCR 방법(RT-PCR 및 nested PCR)과 같은 검출 강도를 가지고 있다. 또한 LAMP 반응을 확인하기 위해 PRMV cDNA을 outer primer sets (Product size 264 bp)로 PCR 한 뒤, Pvu II (CAG/CTG) 제한효소를 처리하였다. 제한효소 처리 결과 2개의 digestion fragments (207 + 57 bp)가 확인되었다. PRMV의 LAMP 진단 방법은 관련 식물로부터 더욱 신속한 모니터링이 가능할 것으로 기대된다.

• 방사성폐기물학회지
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• 제9권3호
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• pp.149-159
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• 2011

원전 운영자는 계통 및 기기의 열화 등에 의해서 발생할 수 있는 비계획적 방사성물질의 방출로 인한 환경 및 주변 주민에의 영향을 합리적으로 달성 가능한 한 낮게 유지하기 위해서 비계획적 방출을 조기에 감지할 수 있는 부지에 적합한 지하수 감시 프로그램을 수립해야 하며, 이를 위해서는 해당 부지의 수문지질 특성의 파악을 통해 지하수 유동을 평가해야 한다. 본 논문에서는 고리 1호기에서 계획되고 있는 지하수 감시 프로그램에 필요한 자료를 제공하기 위해, 고리 1발전소 부지의 기존 수문지질 조사 및 관련 자료의 조사를 통해 부지 내 지하수 유동특성을 파악하고 이를 바탕으로 가상의 비계획적 방출에 의한 삼중수소의 오염운(汚染雲)의 거동을 모의하였다. 모의 결과 지하수의 주 유동 방향은 남서방향이었으며 지하수의 대부분이 남쪽 및 동쪽 바다로 유입되었다. 삼중수소 오염운 역시 바다로 향하였으나 지하집수조(dewatering sump)에 의해 그 속도가 지연되는 것을 확인할 수 있었다.

• 한국안전학회지
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• 제27권5호
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• pp.64-69
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• 2012

The concept of SIL is applied in the most of all standards relating to functional system safety. However there are problems for the people to apply SIL to their plants. as these standards don't include sufficient informations. In this regards, this paper will suggest the direction of SIL application and concept based on IEC 61508 and IEC 61511. A Safety Integrity Level(SIL) is the discrete level(one out of possible fours), corresponding to a range of the probability of an E/E/PE (Electric/Electrical/Programmable Electrical) safety-related system satisfactorily performing the specific safety functions under all the stated conditions within a stated period of time. SIL can be divided into the target SIL(or required SIL) and the result SIL. The target SIL is determined by the risk analysis at the analysis phase of safety lifecycle and the result SIL is calculated during SIL verification at the realization phase of safety lifecycle. The target SIL is determined by the risk analysis like LOPA(Layer Of Protection Analysis), Risk Graph, Risk Matrix and the result SIL is calculated by HFT(Hardware Fault Tolerance), SFF(Safe Failure Fraction) and PFDavg(average Probability of dangerous Failure on Demand). SIL is applied to various areas such as process safety, machinery(road vehicles, railway application, rotating equipment, etc), nuclear sector which functional safety is applied. The functional safety is the part of the overall safety relating to the EUC and the EUC control system that depends on the correct functioning of the E/E/PE safety-related systems and other risk reduction measures. SIL is applied only to the functional safety of SIS(Safety Instrumented System) in safety. EUC is the abbreviation of Equipment Under Control and is the equipment, machinery, apparatus or plant used for manufacturing, process, transportation, medical or other activities.

• 한국농림기상학회지
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• 제13권2호
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• pp.79-86
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• 2011

산간계곡에는 특히 야간에 찬 공기가 흘러 고이는 일이 흔한데, 이런 곳의 식생은 생장과 발육이 그렇지 않은 주변지역에 비해 달라질 수 있어 관심의 대상이 다. 이 연구는 작은 규모의 집수역에서 적외선 영상을 이용하여 냉기호와 온난대의 형상을 가시화 할 수 있는지 알기 위해 수행하였다. 경남 하동군 악양면 형제봉(해발 1,117m)에 적외선 영상 복사계를 설치하여 관측여건이 양호했던 2011년 5월 17일 새벽부터 일출시까지 악양계곡의 열영상을 $640{\times}480$ 해상도로 획득하였다. 열영상 분석결과 계곡의 바닥에 나타난 낮은 온도 분포에 의해 찬공기의 배수를 인지할 수 있었고, 계곡 양쪽 사면에서 바닥보다 $5^{\circ}C$ 높은 온난대의 실체를 확인할 수 있었다. 적외선 영상을 이용하면 기존 고해상도 디지털 기온분포도 '전자기후도'의 신뢰성 검증이 한층 수월해져서 전자기후도 실용성 확보에 도움이 될 것이다.

• 한국압력기기공학회 논문집
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• 제16권1호
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• pp.74-83
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• 2020

A number of so-called 'Type IV' cracking was reported to occur at the welded joints of the P91 steel or P92 steel reheat steam piping systems in Korean supercritical thermal power plants. The reheat steam piping systems are subjected to severe thermal and pressure loading conditions of coolant higher than 570℃ and 4MPa, respectively. In this study, piping analyses and design evaluations were conducted for the piping system of a specific thermal plant in Korea and suggestions were made how structural integrity could be improved so that type IV cracks at the welded joints could be prevented. Integrity evaluations were conducted as per ASME B31.1 code with implicit consideration of creep effects which was used in original design of the piping system and as per nuclear-grade RCC-MRx code with explicit consideration of creep effects. Comparisons were made between the evaluation results from the two design rules. Another approach with modification or reduction of the redundant supports in the piping systems was investigated as a tool to mitigate thermal stresses which should essentially contribute to prevention of Type IV cracking without major modification of the existing piping systems. In addition, a post weld heat treatment method and repair weld method which could improve integrity of the welded joint of P91 steel were investigated.

• Journal of Microbiology and Biotechnology
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• 제28권4호
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• pp.622-629
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• 2018

Expansins are cell wall proteins that mediate cell wall loosening and promote specific tissue and organ morphogenesis in plants and in some microorganisms. Unlike plant expansins, the biological functions of fungal expansin-like proteins have rarely been discussed. In the present study, an expansin-like protein-encoding fvexpl1 gene, was identified from Flammulina velutipes by using local BLAST. It consisted of five exons with a total length of 822 bp. The deduced protein FVEXPL1 contained 274 amino acids with a predicted molecular mass and isoelectric point of 28,589 Da and pH 4.93, respectively. The first 19 amino acids from the N terminal are the signal peptide. Phylogenetic analysis and multiple protein alignment indicated FVEXPL1 was an expansin-like protein. The expression level of fvexpl1 gene in the stipe was significantly higher than that in the mycelia, primordia, and cap. However, the expression level of fvexpl1 gene was significantly higher in the fast elongation region of the stipe as compared with the slow elongation region. Expression analysis indicated that fvexpl1 gene might have an auxiliary role in the stipe morphogenesis of F. velutipes.

• 한국조경학회지
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• 제26권2호
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• pp.194-206
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• 1998

In 1977, Local governments' planting regulations for the development projects were established in accordance with the revised building law in Korea. A landscaped area, planting densities of trees and shrubs, a percentage of evergreen plants, minimum tree size and species were prescribed in the planting regulations. But the clauses for an excessive planting density and a high ratio of evergreen trees that the regulations includes, have been gradually in the way to a creative planting design, and raise a problem of poor growth of trees an a disordered planting landscape. Therefore, in this study the present planting regulations of 124 local governments throughout the country were analyzed and compared with 13 foreign local governments' of 4 countries ; Japan, the United States of America, Canada and Singapore. And the linitations and characteristics of the regulations are drawn as follows ; 1. The regulations focus on controlling the green spaces and plantings by quantitative methods such as controlling the number of trees and the landscaped area, which are inadequate for estimating the crown coverage of mature trees, and which areinadequate for estimating the crown coverage of mature trees, and implementing the function of polantings and the use of green spaces. 2. Minimums of tree densities are higher than those of foreign countries, especially higher about 10 times to 100 times than those of the United States of America. 3.Excessive number of evergreen trees and fruit trees should be planted under the present planting regulations, that results in constricting the creativity in planting design. 4. An article for using specific tree size, 2.0ms over in height makes it difficult to use a wide variety of different sizes of trees. And there is no incentive measures when larger trees are planted. To enhance the quality of green spaces and plantings, it is needed that the function and locating of green spaces and plantings have to be emphasized, and the planting density should be concerned about the mature tree size. The incentive measure to use various sizes of trees is also needed, and the regulations to use excessive number of evergreen trees or fruit trees should be loosened.

• Journal of Microbiology and Biotechnology
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• 제22권9호
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• pp.1193-1201
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• 2012

The analysis and quantification of ammonia-oxidizing bacteria (AOB) is crucial, as they initiate the biological removal of ammonia-nitrogen from sewage. Previous methods for analyzing the microbial community structure, which involve the plating of samples or culture media over agar plates, have been inadequate because many microorganisms found in a sewage plant are unculturable. In this study, to exclusively detect AOB, the analysis was carried out via denaturing gradient gel electrophoresis using a primer specific to the amoA gene, which is one of the functional genes known as ammonia monooxygenase. An AOB consortium (S1 sample) that could oxidize an unprecedented 100% of ammonia in 24 h was obtained from sewage sludge. In addition, real-time PCR was used to quantify the AOB. Results of the microbial community analysis in terms of carbon utilization ability of samples showed that the aeration tank water sample (S2), influent water sample (S3), and effluent water sample (S4) used all the 31 substrates considered, whereas the AOB consortium (S1) used only Tween 80, D-galacturonic acid, itaconic acid, D-malic acid, and $_L$-serine after 192 h. The largest concentration of AOB was detected in S1 ($7.6{\times}10^6copies/{\mu}l$), followed by S2 ($3.2{\times}10^6copies/{\mu}l$), S4 ($2.8{\times}10^6copies/{\mu}l$), and S3 ($2.4{\times}10^6copies/{\mu}l$).

• 상하수도학회지
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• 제31권6호
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• pp.551-566
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• 2017

As the modern society is rapidly developing and people become affluent in materials, many new chemical compounds in different forms of products (e.g., antibiotics, pesticides, detergents, personal care products and plastic goods) are produced, used, and disposed of to the environments. Some of them are persistently having a harmful impact on the environment and mimicking endocrine properties; in general they are present in the environment at low concentrations, so they are called organic pollutants. These organic micropollutants flow to sewage treatment plants via different routes. In this study, the generation characteristics, exposure pathways, detection levels, and environmental impacts of organic micropollutants were critically reviewed. In addition, currently available risk assessment methods and management systems for the compounds were reviewed. The United States Environmental Protection Agency (US EPA), for example, has monitored organic micropollutants and set the monitoring and management of some of the compounds as a priority. To effectively manage organic micropollutants in sewer systems, therefore, we should first monitor organic micropollutants of potential concern and then make a watch list of specific substances systematically, as described in guidelines on listing water pollutants in industrial wastewater.