• Clinical and Experimental Pediatrics
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• v.63 no.3
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• pp.88-95
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• 2020

Accurate localization of the seizure onset zone is important for better seizure outcomes and preventing deficits following epilepsy surgery. Recent advances in neuroimaging techniques have increased our understanding of the underlying etiology and improved our ability to noninvasively identify the seizure onset zone. Using epilepsy-specific magnetic resonance imaging (MRI) protocols, structural MRI allows better detection of the seizure onset zone, particularly when it is interpreted by experienced neuroradiologists. Ultra-high-field imaging and postprocessing analysis with automated machine learning algorithms can detect subtle structural abnormalities in MRI-negative patients. Tractography derived from diffusion tensor imaging can delineate white matter connections associated with epilepsy or eloquent function, thus, preventing deficits after epilepsy surgery. Arterial spin-labeling perfusion MRI, simultaneous electroencephalography (EEG)-functional MRI (fMRI), and magnetoencephalography (MEG) are noinvasive imaging modalities that can be used to localize the epileptogenic foci and assist in planning epilepsy surgery with positron emission tomography, ictal single-photon emission computed tomography, and intracranial EEG monitoring. MEG and fMRI can localize and lateralize the area of the cortex that is essential for language, motor, and memory function and identify its relationship with planned surgical resection sites to reduce the risk of neurological impairments. These advanced structural and functional imaging modalities can be combined with postprocessing methods to better understand the epileptic network and obtain valuable clinical information for predicting long-term outcomes in pediatric epilepsy.

• Journal of muscle and joint health
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• v.13 no.1
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• pp.53-66
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• 2006

Aims. In order to analyze the concept of stigma, so to develop a valid instrument to measure stigma. Methods. First, a concept analysis was conducted with the aim of clarifying the state of the science of discipline-specific conceptualizations of stigma. The criterion-based method of concept analysis as described by Morse and colleagues was used (Morse et al., 1996; Morse, 2000). This analytic process enabled the assessment of the scientific maturity of the concept of stigma. The interdisciplinary concept of stigma was found to be immature. Based on this level of maturity it was determined that in order to advance the concept of stigma toward gloater maturity. techniques of concept development using the literature as data were applied. In this process, questions were 'asked of the data' (in this case, the selected disciplinary literatures) to identify the conceptual components of stigma. Results. The inquiry into the concept of stigma led to the development of an expanded interdisciplinary conceptual definition by merging the most coherent commonalties from each discipline. And the conceptual components of stigma were identified. The antecedent factors of stigma were "apart from social identity". The attributes of stigma were "devaluing, labeling, negative stereotypes, discrimination". The consequences of stigma were "social rejection, social isolation, deficiency of social support, low social status".

• Proceedings of the Korean Society of Organic Agriculture Conference
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• 2009.07a
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• pp.185-197
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• 2009

Eco-friendly agricultural products(EFAPs) are on an increasing trend of supply, but not much enhancing the rate of consumption due to higher price premiums and safety concerns among core consumers. Recently, the overall domestic market is rapidly changing from supplier's into a consumer-oriented one with increasing preferences for well-known branded produce, large retailers, and e-commerce. To cope with marketing shifts and develop eco-friendly farming, marketing strategies should be properly prepared on the basis of consumption trends, purchase patterns, and the specific needs which is interlinked sectors of productions, distributions, and general process of consumption. The key factors for stimulating the demand for EFAPs are outlined as follows : classification of buyers(segmentation of markets), provision of more information and safety assurance, labeling of brands and reference prices, shopping convenience for consumers and more appropriate pricing. In order to motivate consumers to buy more EFAPs, the products' safety should be verified and they should be made to sell at specialty store such as Chorokmaul, It is necessary that government, producers, consumers, and distributors play their respective roles in developing feasible programs for marketing EFAPs. Consequently, the specialty store will greatly contribute to the development of domestic environmentally-friendly farming and to raising their standings and prospects as well.

• Journal of the Korean Society of Industry Convergence
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• v.23 no.3
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• pp.481-489
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• 2020

Safety and security during the handling of hazardous materials is a great concern for anyone in the field. One driving point in the security field is the ability to detect the source of the danger and take action against it as quickly as possible. Via the usage of a fully convolutional network, it is possible to create the label map of an input image, indicating what object is occupying the specific area of the image. This research employs the usage of U-net, which was constructed in biomedical field segmentation to segment cells, instead of the original FCN. One of the challenges that this research faces is the availability of ground truth with precise labeling for the dataset. Testing the network after training resulted in some images where the network pronounces even better detail than the expected label map. With better detailed label map, the network might be able to produce better segmentation is something to be studied in further research.

• Journal of Microbiology and Biotechnology
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• v.19 no.9
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• pp.904-910
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• 2009

The development of effective cellular imaging requires a specific labeling method for targeting, tracking, and monitoring cellular/molecular events in the living organism. For this purpose, we studied the cellular uptake of isocyanide-functionalized silver and gold nanoparticles by surface-enhanced Raman scattering (SERS). Inside a single mammalian cell, we could monitor the intracellular behavior of such nanoparticles by measuring the SERS spectra. The NC stretching band appeared clearly at ${\sim}2,100cm^{-1}$ in the well-isolated spectral region from many organic constituents between 300 and 1,700 or 2,800 and $3,600cm^{-1}$. The SERS marker band at ${\sim}2,100cm^{-1}$ could be used to judge the location of the isocyanide-functionalized nanoparticles inside the cell without much spectral interference from other cellular constituents. Our results demonstrate that isocyanide-modified silver or gold nanoparticle-based SERS may have high potential for monitoring and imaging the biological processes at the single cell level.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.2
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• pp.831-836
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• 2015

Tumor fluid accumulation occurs in both human cancer and experimental tumor models. Solid tumors show a tendency to tumor fluid accumulation because of their anatomical and physiological features and this may be influenced by molecular factors. Fluid accumulation in the peri-tumor area also occurs in the Dunning model of rat prostate cancer as the tumor grows. In this study, the effects of tumor fluids that were obtained from Dunning prostate tumor-bearing Copenhagen rats on the strongly metastatic MAT-LyLu cell line were investigatedby examining the cell's migration and tumor fluid's toxicity and the kinetic parameters such as cell proliferation, mitotic index, and labelling index. In this research, tumor fluids were obtained from rats injected with $2{\times}10^5$ MAT-LyLu cells and treated with saline solution, and 200 nM tetrodotoxin (TTX), highly specific sodium channel blocker was used. Sterilized tumor fluids were added to medium of MAT-LyLu cells with the proportion of 20% in vitro. Consequently, it was demonstrated that Dunning rat prostate tumor fluid significantly inhibited proliferation (up to 50%), mitotic index, and labeling index of MAT-LyLu cells (up to 75%) (p<0.05) but stimulated the motility of the cells in vitro.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.2 no.2
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• pp.113-117
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• 2016

As a neurotransmitter, serotonin plays important roles in brain. It relates various neuropsychiatric disorders such as anxiety, depression, schizophrenia. [$^{11}C$]DASB is a well-known PET tracer for serotonin transporter imaging. In this study, we synthesized [$^{11}C$]DASB in HPLC loop for simple and rapid production. Total synthesis time was about 40 minutes and the radiochemical purities were over 99%. The specific activity was $51.4GBq/{\mu}mole$ (n=16). [$^{11}C$]DASB showed highest uptake in mid-brain that serotonergic nerves are abundant and lowest uptake in cerebellum. In conclusion, we used HPLC loop method for [$^{11}C$]DASB labeling and this method is useful for production of $^{11}C$ labeled PET tracers.

• Journal of Institute of Control, Robotics and Systems
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• v.18 no.12
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• pp.1122-1131
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• 2012

This paper presents obstacle avoidance method for scout robot or industrial robot in unknown environment by using IR sensor and vision system. In the proposed method, robots share the information where the obstacles are located in real-time, thus the robots can choose the best path for obstacle avoidance. Using IR sensor and vision system, multiple robots efficiently evade the obstacles by the proposed cooperation method. No landmark is used at wall or floor in experiment environment. The obstacles don't have specific color or shape. To get the information of the obstacle, vision system extracts the obstacle coordinate by using an image labeling method. The information obtained by IR sensor is about the obstacle range and the locomotion direction to decide the optimal path for avoiding obstacle. The experiment was conducted in $7m{\times}7m$ indoor environment with two-wheeled mobile robots. It is shown that multiple robots efficiently move along the optimal path in cooperation with each other in the space where obstacles are located.

• Korean Journal of Veterinary Pathology
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• v.1 no.2
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• pp.119-124
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• 1997

The objective of this study was to develop digoxigenin (DIG)-labeled in situ hybridization (ISH) test for diagnosis of Aujeszky's Disease(AD) in infected organs. Specific DNA with well conserved gene sequences encoding gp50 antigen in AD virus (ADV) was obtained by Polymerase Chain Reaction (PCR) method. A pair of oligonucleotide primers used in PCR allowed amplification of a 217 bp sequence from the gp50 ADV gene. The DNA was then labeled with DIG by primer labeling method for use as probe in ISH test to detect ADV nucleic acids in various tissue. Positive hybridization was demonstrated by dark pigmentation in nuclei and cytoplasm of ADV infected cells particularly in brain tonsillar crypt epithelium and pulmonary alveolar cells. This result suggests that ISH is a valuable sensitive and rapid diagnostic test for AD.

• Journal of the Institute of Convergence Signal Processing
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• v.1 no.1
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• pp.89-96
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• 2000

In this paper, we propose a vision algorithm for the inspection of very small RF-chip inductor which is used in mobile-communication terminal. The proposed method divides coil part from the inductor body by local adaptive thresholding and integral projection method. After dividing work, the coil components are extracted by thinning and labelling techniques. The test items are the number of turns, the intervals in coil, and the measure of uniformity between the extracted lines. If the values of these are more than the specific value a tested product is decided bad one. In the simulation, the proposed method has a good performance.