• Title/Summary/Keyword: species delimitation

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Community Structures of Macrobenthos in Chonsu Bay, Korea

  • Lee, Jae-Hac;Park, Heung-Sik
    • Journal of the korean society of oceanography
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    • v.33 no.1-2
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    • pp.18-27
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    • 1998
  • Based on 53 quantitative samples collected in April (23 stations) and August (30 stations) of 1993, the species composition, distribution and abundance of macrobenthic invertebrates and bottom characteristics in Chonsu Bay were conducted. Bottom sediments consisted of mud at the close to the dyke, sandymud in the central bay, and coarse sand in the mouth of the bay. A total of 273 species (177 species in April and 200 species in August) were identified. Mean density per sampling station was 480 ind./m$^2$ in April and 1126 ind./m$^2$ in August, respectively. Number of species and densities decreased in the northern part of the bay. The macrobenthos showed a temporal difference in species composition, range of distribution, and community delimitation caused by larval settling. Although during the settling period in summer, large numbers of juveniles added in most of regions, unrelated to environmental conditions, but, successful recruitment may has been a rare event locally Theora fragilis (bivalve), Lumbrineris longifolia(polychaete), Neptys oligobranchia (polychaete) were numerically dominant, but their densities varied seasonally. Community structures of macrobenthos in Chonsu Bay were affected by several bottom environmental conditions, related to tidal current speed, regionally.

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Wood anatomy of Korean Symplocos Jacq. (Symplocaceae)

  • GHIMIRE, Balkrishna;PARK, Beom Kyun;OH, Seung-Hwan;LEE, Jaedong;SON, Dong Chan
    • Korean Journal of Plant Taxonomy
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    • v.50 no.3
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    • pp.333-342
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    • 2020
  • Despite poorly documented species delimitation and unresolved taxonomic nomenclature, four species of Symplocos (S. coreana, S purnifolia, S sawafutagi, and S. tanakana) have been described in Korea. In this study, we carried a comparative wood anatomy analysis of all four species of Korean Symplocos to understand the wood anatomical variations among them. The results of this study indicated that Korean Symplocos are comparatively indistinguishable in terms of their qualitative wood features, except for exclusively uniseriate rays present in S. purnifolia instead of uniseriate to multiseriate in other three species. Nevertheless, differences are noticed in quantitative wood variables such as the vessel density, vessel size, and ray density. The vessel density of S. purnifolia is more than twice as high as those of S. sawafutagi and S. tanakana. In contrast, the vessel circumference and diameter on both plants of S. sawafutagi and S. tanakana is nearly twice as large as those of S. purnifolia. Symplocos coreana has characteristic intermediacy between these two groups in terms of vessel features and is closer to S. purnifolia in terms of its ray density level. A cluster analysis based on a paired group (unweighted pair-group method with the arithmetic mean, UPGMA) algorithm using the Euclidean similarity index clearly differentiates S. purnifolia from the remaining species, representing the first branch of the phenogram.

Kordyana commelinae Associated with White Smut-like Disease on Commelina communis and C. minor in Korea

  • Park, Ji-Hyun;Jung, Bok-Nam;Choi, In-Young;Shin, Hyeon-Dong
    • Mycobiology
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    • v.49 no.3
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    • pp.275-279
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    • 2021
  • A fungus of the genus Kordyana, found on leaves of Commelina communis and C. minor exhibiting white smut-like symptoms, was identified as Kordyana commelinae based on morphological characteristics and two rDNA sequence analyses. We report the novel occurrence of the genus Kordyana in Korea and the association of K. commelinae with the host plant species. As well, we provide the necessary mycological information to resolve species delimitation and taxonomic problems of Kordyana.

DNA Barcoding of Antarctic Freshwater Copepod Boeckella poppei (Crustacea: Copepoda: Calanoida: Centropagidae) Inhabiting King George Island, South Shetland Islands, Antarctica

  • Kang, Seunghyun;Jo, Euna
    • Animal Systematics, Evolution and Diversity
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    • v.36 no.4
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    • pp.396-399
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    • 2020
  • The Antarctic freshwater copepod, Boeckella poppei (Mrazek, 1901), has the widest range of distribution extending from southern South America to Antarctic continent, among all Boeckella species. Boeckella poppei is the only freshwater copepod known to be inhabiting the Antarctic continent. In present study, we analyzed the DNA barcodes of the mitochondrial cytochrome c oxidase subunit I (COI) gene of B. poppei from King George Island, Antarctica. The intraspecific genetic distances varied from 0% to 13% and interspecific genetic distances ranged from 11% to 14%. The overlap of DNA barcode gap suggests careful threshold-based delimitation of species boundaries.

Accurate Delimitation of Phanerochaete chrysosporium and Phanerochaete sordida by Specific PCR Primers and Cultural Approach

  • Lim, Young-Woon;Baik, Keun-Sik;Chun, Jong-Sik;Lee, Kang-Hyun;Jung, Won-Jin;Bae, Kyung-Sook
    • Journal of Microbiology and Biotechnology
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    • v.17 no.3
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    • pp.468-473
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    • 2007
  • White rot fungi, Phanerochaete chrysosporium and Phanerochaete sordida, have been mostly studied in a variety of industrial processes like biopulping and pulp bleaching as well as in bioremediation. Whereas P. sordida is widely distributed in the North Temperate Zone, P. chrysosporium is reported in the restricted area and hundreds of reports have been described from a few strains of P. chrysosporium, which are deposited at various fungal collections in the world. The isolates of two species are not easily discriminated because of their morphological and molecular similarity. Through the ITS sequence analyses, a region containing substantial genetic variation between the two species was identified. PCR amplification using two specific primers was successfully used to differentiate P. chrysosporium from P. sordida. These results were supported by cultural studies. The growth rates at $37^{\circ}C$ on PDA, MEA, and Cza and the microscopic features of conidia on PDA and YMA were also very useful to differentiate those two species.

Development of microsatellite markers for Hosta capitata (Asparagaceae) and amplification in related taxa

  • CHOI, Mi-Jung;LEE, Jung-Hyun;CHO, Won-Bum;HAN, Eun-Kyeong;CHOI, Hyeok-Jae
    • Korean Journal of Plant Taxonomy
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    • v.50 no.3
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    • pp.327-332
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    • 2020
  • Microsatellite markers were developed as a tool for phylogeographic studies of Hosta capitata. We also assessed cross-amplification in species closely related to Hosta capitata. We produced 28 polymorphic microsatellite markers by mapping 300 bp paired-end reads obtained from Illumina MiSeq data of H. capitata. In H. capitata, the number of alleles per locus ranged from 1 to 13. Observed and expected heterozygosity ranged from 0.000 to 0.844 and 0.000 to 0.832, respectively. Additionally, 13 loci were successfully transferable to the related species of H. minor and H. venusta. These markers will provide a powerful genetic tool not only for elucidating the phylogeographic patterns of H. capitata populations but also for studying the genetic delimitation of H. capitata from its related species.

A Morphological Study of Symplocaceae in Korea (한국산 노린재나무과의 형태학적 연구)

  • Park, Sang-Hong;Lee, Joongku;Kim, Joo-Hwan
    • Korean Journal of Plant Taxonomy
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    • v.37 no.3
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    • pp.255-273
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    • 2007
  • The morphological characters for 4 taxa of Korean Symplocaceae were examined. Based on morphological examination, numerical analysis was performed to clarify the taxonomic relationships among the taxa. It was found that epidermal characters of leaves, cellular deposition of petals, cellular boundary of petioles, growth of stigmas and pollen grains and their surfaces were useful diagonostic characters. From the results of PCA analysis, four taxa were grouped as species clusters including each populations. Four species of Symplocos were clustered as species groups with clear delimitation. Morphological, numerical, and palynological analyses supported the previous morphological studies on this family.

A Systematic Study on the Cimicifuga foefida L Complex and Related Species (Ranunculaceae) (Cimicifuga foetida L. Complex 및 근연종(미나리아재비과)에 대한 분류학적 연구)

  • 박종욱
    • Journal of Plant Biology
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    • v.37 no.1
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    • pp.111-124
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    • 1994
  • The delimitation and systematic relationships of the C. foetida complex and related species were evaluated by numerical methods, including principal components analysis and cluster analysis of major morphological characters. Results of these analyses strongly support recognition of three varieties in C. foetida; these include C. foetida var. foetida, C. foetida var. micrantha, and C. foetida var. velutita. The results also indicated that C. brachycarpa, C. frigida, C. heracleifolia, and C. yunnanensis are distinct species, and C. heracleifolia comprises C. heracleifolia var. heracleifolia and C heracleifolia var. bifida. Cimicifuga mairei and C. foetida var. foliolosa, however, appeared to be morphologically indistinguishable from C. foetida var. velutina. In addition, C. foetida var. longibracteata and C. foetida var. bifula are conspecific with C. frigida.rigida.

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Polyphasic delimitation of a filamentous marine genus, Capillus gen. nov. (Cyanobacteria, Oscillatoriaceae) with the description of two Brazilian species

  • Caires, Taiara A.;Lyra, Goia de M.;Hentschke, Guilherme S.;da Silva, Aaron Matheus S.;de Araujo, Valter L.;Sant'Anna, Celia L.;Nunes, Jose Marcos de C.
    • ALGAE
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    • v.33 no.4
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    • pp.291-304
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    • 2018
  • Lyngbya C. Agardh ex Gomont is a nonheterocytous cyanobacterial genus whose evolutionary history is still poorly known. The traditionally defined Lyngbya has been demonstrated to be polyphyletic, including at least five distinct clades, some of which have been proposed as new genera. Intraspecific diversity is also clearly underestimated in Lyngbya due to the lack of unique morphological characters to differentiate species. In this study, we describe the new genus Capillus T. A. Caires, C. L. Sant'Anna et J. M. C. Nunes from benthic marine environments, including two new Brazilian species (here described as C. salinus T. A. Caires, C. L. Sant'Anna et J. M. C. Nunes, and C. tropicalis T. A. Caires, C. L. Sant'Anna et J. M. C. Nunes), and two species yet to be described, one of them from India (Capillus sp. 2.1), and the other from United States of America, based on strain PCC 7419. Capillus species presented cross-wise diagonal fragmentation, assisted or not by necridic cells, which has not been previously mentioned for Lyngbya. Ultrastructural analyses showed that C. salinus and C. tropicalis have numerous gas vesicles, which are rarely described for benthic marine species. The new genus formed a well-supported clade, and the D1-D1' and Box B secondary structures of internal transcribed spacer also supported the proposal of its new species. These findings help to clarify the diversity of species in the Lyngbya complex and the taxonomy of the group, and highlight the need of further floristic surveys in tropical coastal environments, which remain poorly studied.

Amazonocrinis thailandica sp. nov. (Nostocales, Cyanobacteria), a novel species of the previously monotypic Amazonocrinis genus from Thailand

  • Tawong, Wittaya;Pongcharoen, Pongsanat;Pongpadung, Piyawat;Ponza, Supat;Saijuntha, Weerachai
    • ALGAE
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    • v.37 no.1
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    • pp.1-14
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    • 2022
  • Cyanobacteria are distributed worldwide, and many new cyanobacterial species are discovered in tropical region. The Nostoc-like genus Amazonocrinis has been separated from the genus Nostoc based on polyphasic methods. However, species diversity within this genus remains poorly understood systematically because only one species (Amazonocrinis nigriterrae) has been described. In this study, two novel strains (NUACC02 and NUACC03) were isolated from moist rice field soil in Thailand. These two strains were characterized using a polyphasic approach, based on morphology, 16S rRNA phylogenetic analysis, internal transcribed spacer secondary structure and ecology. Phylogenetic analyses based on 16S rRNA gene sequences confirmed that the two novel strains formed a monophyletic clade related to the genus Amazonocrinis and were distant from the type species A. nigriterrae. The 16S rRNA gene sequence similarity (<98.1%) between novel strains and all other closely related taxa including the Amazonocrinis members exceeded the cutoff for species delimitation in bacteriology, reinforcing the presence of a new Amazonocrinis species. Furthermore, the novel strains possessed unique phenotypic characteristics such as the presence of the sheath, necridia-like cells, larger cell dimension and akinete cell arrangement in long-chains and the singularity of D1-D1', Box-B, V2, and V3 secondary structures that distinguished them from other Amazonocrinis members. Considering all the results, we described our two strains as Amazonocrinis thailandica sp. nov. in accordance with the International Code of Nomenclature for Algae, Fungi and Plants.