• Mycobiology
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• v.31 no.4
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• pp.209-213
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• 2003

Aspergillus flavus K-03 isolated from poultry forming soil in Korea was studied for its ability to produce extracellular proteases on basal medium containing 2%(w/v) chicken feathers. The fungus was observed to be a potent producer of such enzymes. Keratinolytic enzyme secretion was the best at 15 days of incubation period at pH 9 and temperature $40^{\circ}C$. No relationship existed between the enzyme yield and increase of biomass. Enzyme production was suppressed by exogenous sugars in descending order arabinose>maltose>mannose>fructose. But glucose did not influence the enzyme activity. The keratinolytic enzyme released by the fungus demonstrated the ability to decompose keratin substrates as chicken feather when exogenous glucose was present. The keratinolytic activity was inhibited by $HgCl_2$ and serine-protease inhibitors such as phenymethylsulfonyl fluoride(100%), chymostain(88%), crystalline soybean trypsin inhibtor(80%), antipain(45%) and aprotinin(40%), and was not by cystein-protease and aspartyl-protease inhibitors. The enzyme activity is only partially inhibited by metallo-protease inhibitor. Thus, the enzyme secreted by A. flavus K-03 belongs to the alkaline serine-type protease.

• Plant Resources
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• v.8 no.2
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• pp.116-121
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• 2005

Compositae plants are known to contain biologically active substances that are allelopathic to agricultural crops as well as weed species. Aqueous or methanol extracts and plant residues from leaves of Xanthium occidentale were assayed against alfalfa (Medicago sativa) to determine its allelopathic effects, and the results showed that the extracts applied onto filter paper significantly inhibited seed germination as well as root growth of alfalfa. Aqueous leaf extracts of $40g\;L^{-1}$ were completely inhibitory on root growth of alfalfa, while root growths of barley (Hordeum vulgare L.) and soybean (Glycine max L.) were less sensitive. Leaf residue incorporation at $100g\;kg^{-1}$ into soil on seedling growth of barnyard grass (Echinochloa crus-galli Beauv. var. oryzicola Ohwi) inhibited both shoot and root fresh weights of barnyard grass by 94 and $96\%$, respectively. Methanol extracts from BuOn and EtOAc fractions of X. occidentale reduced alfalfa root growth more than did those from hexane and water fractions. The results based on bioassay of extracts and residues show that X. occidentale had potent an allelopathic activity against other plant species.

• Proceedings of the Korean Society of Life Science Conference
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• 2001.09a
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• pp.52-52
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• 2001

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.10a
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• pp.170-170
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• 2018

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.5
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• pp.674-683
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• 2010

The current study was performed to examine the agricultural suitability of the cultivated upland nearby abandoned mining areas in Korea using three different scientific risk assessment models of Korea, USA and UK. For this, three mining sites DM, MG and KS were selected among 687 abandoned mines through preliminary risk assessment. A wide range of parameters were obtained through analysis of both soil and crop samples from the selected areas for heavy metal concentration and questionnaires to the communities along with the selected mining sites. Heavy metal concentration in soil samples was lower than the values previously reported by the Ministry of Environment (ME, 2002). However, both As and Cd concentration in the soil samples exceeded the concern level for agricultural area of the Soil Environment Conservation Act. Judging from the contaminant criteria for the crops, only Zn level in pepper, soybean and corn from the mining area DM exceeded the criteria whereas As, Cd, $Cr^{6+}$, Cu, Hg, Ni, Pb did not exceed the criteria. It was demonstrated that there would be human health risk by Pb accumulated in crops from both mining areas MG and KS when estimated by the risk assessment models of Korea and USA. Against it, results of the risk assessment model of UK showed human health risk by Pb in the crops from all study areas.

• Korean Journal of Agricultural Science
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• v.14 no.1
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• pp.68-80
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• 1987

This study was carried out to investigate the evapotranspiration and variations of soil moisture contents for soybeans. The relationship between actual evapotranspiration obtained by the water balance equation and estimated evapotranspiration obtained by the soil moisture model was analyzed. The results obtained were summarized as follows; 1. The total amount of actual evapotranspiration of soybeans during growing season was 405.7mm. The total amount of reference crop evapotranspiration of soybeans that was estimated by Pan evaporation and Hargreaves method were 547.8 mm and 586.8 mm, respectively. Crop coefficient during growing season were shown on Table 1. 2. Measured actual evapotranspiration of soybean during growing season was 405.7 mm and estimated actual evapotranspiration by pan evaporation and Hargreaves method were 424.7 mm, and 426.1mm, r3 respectively. 3. The variations of soil moisture content for soybeans were high at 10cm layer, as compared with those at 30cm and 50cm layers. Because discrepancy between the variations of soil moisture content predicted by model and observed by soil moisture meter was still great, it is required to study the consumptive types of soil moisture at each root depth.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.127-134
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• 2012

Microwave remote sensing can help monitor the land surface water cycle, crop growth and soil moisture. A ground-based polarimetric scatterometer has an advantage for continuous crop using multi-polarization and multi-frequencies and various incident angles have been used extensively in a frequency range expanding from L-band to Ka-band. In this study, we analyzed the relationships between L-, C- and X-band signatures and soil moisture content over the whole soybean growth period. Polarimetric backscatter data at L-, C- and X-bands were acquired every 10 minutes. L-band backscattering coefficients were higher than those observed using C- or X-band over the period. Backscattering coefficients for all frequencies and polarizations increased until Day Of Year (DOY) 271 and then decreased until harvesting stage (DOY 294). Time serious of soil moisture content was not a corresponding with backscattering over the whole growth stage, although it increased relatively until early August (R2, DOY 224). We conducted the relationship between the backscattering coefficients of each band and soil moisture content. Backscattering coefficients for all frequencies were not correlated with soil moisture content when considered over the entire stage ($r{\leq}0.50$). However, we found that L-band HH polarization was correlated with soil moisture content (r=0.90) when Leaf Area Index (LAI)<2. Retrieval equations were developed for estimating soil moisture content using L-band HH polarization. Relation between L-HH and soil moisture shows exponential pattern and highly related with soil moisture content ($R^2=0.92$). Results from this study show that backscattering coefficients of radar scatterometer appear effective to estimate soil moisture content.

• Korean Journal of Soil Science and Fertilizer
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• v.27 no.2
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• pp.65-71
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• 1994

This study was conducted to investigate the influence of treatment of fly ash on heavy metal contents in the arable soils. Rice was cultivated on the two types of paddy field(clay loam and sandy loam soil) with 0, 4, 8, 12t/10a of anthracite fly ash and bituminous coal fly ash, respectively. And soybean was cultivated on the same types of upland field with those of 0, 3, 6, 9t/10a, respectively. At the harvest time, the heavy metal contents in surface and subsoil were investigated. The results were summarized as follows : 1. Anthracite fly ash. 1) In the paddy field of clay loam, the contents of Cu and Zn in the surface soil and Cd and Ni in the subsoil were increased with the increase of the amount of fly ash applied, but the others didn't show that tendency. 2) In the paddy field of sandy loam, only the content of Fe was increased in the surface and subsoils. 3) In the case of upland soil, the concentration of Ni and Cr in the surface soil and Cd in the subsoil were increased in the clay loam soil, and those of Cr in the surface soil and Pb in the subsoil were increased in the sandy loam soil. 2. Bituminous coal fly ash 1) In the paddy field of clay loam, the contents of Cu and Zn in the subsoil were increased with increase of the amount of fly ash applied, but in the case of sandy loam, those of Pb and Ni in the surface soil were increased. 2) In the upland soil of clay loam, the concentration of Ni in the surface soil and Pb in the subsoil were increased. 3) In case of upland soil of sandy loam, the contents of Cr and Fe were increased in the surface and subsoil, respectively, but those of Cu and Mn were increased in the both of the surface and subsoil.

• Korean Journal of Soil Science and Fertilizer
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• v.24 no.1
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• pp.61-68
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• 1991

Thirty Bradyrhizobium japonicum isolates (10 strains per each soil) from 1 uncultivated [Sangnam(Soil 1), Milyang]- and 2 cultivated [Dong(Soil 2)and Chinbuk(Soil 3), Changweon] upland soils in Yeongnam area were evaluated on their symbiotic effectiveness to soybean [Glycin max (L.)] cv. Korean Jangbaekkong and American Clark and examined on their serological diversity. The results obtained were summarized as follows : 1. On symbiotic effectiveness of B. japonicum with plant genotypes, isolates showed a relatively high value of nodule mass in Jangbaekkong cv. and of shoot dry weight and total nitrogen in Clark cv. demonstrating the order of Soil 1> Soil 2> Soil 3 isolates. 2. Among 30 B. japonicum isolates, YCK 141 showed the best effectiveness on mean nitrogen fixation of two cultivars. 3. Thirty indigenous B. japonicum showed 6 types of serological diversities in the immunoblot analysis which were present in various proportions at Soil 2(5) and Soil 3(5) except Soil 1 where all isolates fell into the YCK 117 serogroup. And their distribution order was serotype YCK 117( 12 strains) > USDA 1l0(5strains), USDA 123(5 strains) > YCK 150(4 strains) > YCK 141(3 strains) > YCK 226(1 strain). 4. Especially, 10 isolates from Soil 1, an uncultivated orchard, showed a very homologous pattern in not only effectiveness but serological distribution. It seemed to indicate that the isolates were typically affected by numerous physical and environmental factors of the soil.

• 한국균학회소식:학술대회논문집
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• 2014.05a
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• pp.25-25
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• 2014

Koji molds are comprised of yellow, black and white. Black and white koji molds were recently re-visited by this author and it is concluded that they consists of Aspergillus luchuesnsis, A. niger and A. tubingensis, and the most important species for alcoholic beverage production is A. luchuensis. In the case of yellow koji mold, it is comprised of Aspergillus oryzae, A. sojae and A. tamari. In the case of A. sojae, the species is scarcely isolated from nature and rarely used for industry in Korea. Aspergillus tamari is often isolated from traditional Korean Meju, a fermented soybean product, and the classification of the species is clear. However, in the case of A. oryzae, differentiation between A. oryzae and A. flavus is still in controversy. In this study, we collected 415 strains of Aspergillus flavus/oryzae complex from air, rice straw, soybean, corn, peanut, arable soil and Meju in Korea and we examined the aflatoxin producing capacity of the strains. The norB-cypA, omtA and aflR genes in the aflatoxin biosynthesis gene cluster were analyzed. We found that 367 strains (88.4%) belonged to non-aflatoxigenic group (Type I of norB-cypA, IB-L-B-, IC-AO, or IA-L-B- of omtA, and AO type of aflR), and only 48 strains (11.6%) belonged to aflatoxin-producible group (Type II of norB-cypA, IC-L-B+/B- or IC-L-B+ of omtA, and AF type of aflR). In the case of A. flavus/oryzae strains from Meju, almost strains (178/192, 92.7%) belonged to non-aflatoxigenic group and only 14 strains (7.3 %) belonged to aflatoxin-producible group. It is proposed in this study that non-aflatoxigenic strain from Meju is classified as A. oryzae, considering that Meju is food material.