• JSTS:Journal of Semiconductor Technology and Science
• /
• v.2 no.1
• /
• pp.19-29
• /
• 2002

A simple and analytical design approach for input power matched CMOS RF LNA circuits and their scaling for lower power consumption, is introduced. In spite of the simplicity of our expressions, it gives excellent agreement with numerical simulation results using commercial CAD tools for several circuit examples performed at 2.4GHz using $0.18\mu\textrm{m}$ CMOS technology. These simple and analytical results are extremely useful in that they can provide enough insights not only for designing any CMOS LNA circuits, but also for characterizing and diagnosing them whether being prototyped or manufactured.

• Journal of Korean Neurosurgical Society
• /
• v.53 no.4
• /
• pp.207-212
• /
• 2013

Objective : Recent studies have shown encouraging progress toward the use of autogenic and allogenic mesenchymal stem cells (MSCs) to arrest, or even lead to partial regeneration in, intervertebral disc (IVD) degeneration. However, this technology is still in its infancy, and further development is required. The aim of this study was to analyze whether rat adipose-derived mesenchymal stem cells (ADMSC) can differentiate towards IVD-like cells after treatment with transforming growth factor ${\beta}3$ (TGF-${\beta}3$) in vitro. We also performed quantitative analysis of gene expression for ADMSC only, ADMSCs treated with TGF-${\beta}3$, and co-cultured ADMSCs treated with TGF-${\beta}3$. Methods : ADMSCs were sub-cultured to homogeneity and used in fluorocytometry assays for CD11, CD45, and CD90/Thy1. ADMSCs were differentiated in spheroid culture towards the chondrogenic lineage by the presence of TGF-${\beta}3$, dexamethasone, and ascorbate. We also co-cultured pure ADMSCs and nucleus pulposus cells in 24-well plates, and performed immunohistochemical staining, western blotting, and RT-PCR for quantitative analysis of gene expression. Results : Results of fluorocytometry were positive for CD90/Thy1 and negative for CD11 and CD45. TGF-${\beta}3$-mediated induction of ADMSCs led to the expression of the differentiation markers of intervertebral disc-like cells, such as aggrecan, collagen II, and sox-9. Co-cultured ADMSCs treated with TGF-${\beta}3$ showed higher expression of differentiation markers and greater extracellular matrix production compared with ADMSCs treated with TGF-${\beta}3$ alone. Conclusion : ADMSC treated with TGF-${\beta}3$ may be an attractive source for regeneration therapy in degenerative IVD. These findings may also help elucidate the pathologic mechanism of MSC therapy in the degeneration of IVD in vivo.

• BMB Reports
• /
• v.53 no.2
• /
• pp.65-73
• /
• 2020

Life expectancy has dramatically increased around the world over the last few decades, and staying healthier longer, without chronic disease, has become an important issue. Although understanding aging is a grand challenge, our understanding of the mechanisms underlying the degeneration of cell and tissue functions with age and its contribution to chronic disease has greatly advanced during the past decade. As our immune system alters with aging, abnormal activation of immune cells leads to imbalance of innate and adaptive immunity and develops a persistent and mild systemic inflammation, inflammaging. With their unique therapeutic properties, such as immunomodulation and tissue regeneration, mesenchymal stem cells (MSCs) have been considered to be a promising source for treating autoimmune disease or as anti-aging therapy. Although direct evidence of the role of MSCs in inflammaging has not been thoroughly studied, features reported in senescent MSCs or the aging process of MSCs are associated with inflammaging; MSC niche-driven skewing of hematopoiesis toward the myeloid lineage or oncogenesis, production of pro-inflammatory cytokines, and weakening their modulative property on macrophage polarization, which plays a central role on inflammaging development. This review explores the role of senescent MSCs as an important regulator for onset and progression of inflammaging and as an effective target for anti-aging strategies.

• Journal of Biomedical Engineering Research
• /
• v.38 no.5
• /
• pp.256-263
• /
• 2017

By using the buildup characteristics of the radiophotoluminescence glass dosimeter(RPLGD), it is aimed to help the measurement of the accurate dose by measuring the radiation dose according to the time of the glass element. Five glass elements were arranged on the table and the source to image receptor distance(SID) was set to 100 cm for the build-up radiation dose measurement of the fluorescent glass dosimeter glass element(GD-352M). Radiation doses and saturation rates were measured over time according to irradiation time, with the tube voltage (30, 60, 90 kVp) and tube current (50, 100 mAs) Repeatability test was repeated ten times to measure the coefficient of variation. The radiation dose increased from 0.182 mGy to 12.902 mGy and the saturation rate increased from 58.3% with increasing exposure condition and time. The coefficient of variation of the glass elements of the fluorescent glass dosimeter was ranged from 0.2 to 0.77 according to the X - ray exposure conditions. X - ray exposure showed that the radiation dose and saturation rate were increased with buildup characteristics, and degeneration of glass elements was not observed. The reproducibility of the variation coefficient of the radiation generator was included within the error range and the reproducibility of the radiation dose was excellent.

• BMB Reports
• /
• v.47 no.3
• /
• pp.135-140
• /
• 2014

The mesenchymal stem cells (MSCs), which are derived from the mesoderm, are considered as a readily available source for tissue engineering. They have multipotent differentiation capacity and can be differentiated into various cell types. Many studies have demonstrated that the MSCs identified from amniotic membrane (AM-MSCs) and amniotic fluid (AF-MSCs) are shows advantages for many reasons, including the possibility of noninvasive isolation, multipotency, self-renewal, low immunogenicity, anti-inflammatory and nontumorigenicity properties, and minimal ethical problem. The AF-MSCs and AM-MSCs may be appropriate sources of mesenchymal stem cells for regenerative medicine, as an alternative to embryonic stem cells (ESCs). Recently, regenerative treatments such as tissue engineering and cell transplantation have shown potential in clinical applications for degenerative diseases. Therefore, amnion and MSCs derived from amnion can be applied to cell therapy in neuro-degeneration diseases. In this review, we will describe the potential of AM-MSCs and AF-MSCs, with particular focus on cures for neuronal degenerative diseases.

• Journal of the Korean Society for Industrial and Applied Mathematics
• /
• v.26 no.2
• /
• pp.108-120
• /
• 2022

We considered qualitative behaviour of the generalization of Einstein's model of Brownian motion when the key parameter of the time interval of free jump degenerates. Fluids will be characterised by number of particles per unit volume (density of fluid) at point of observation. Degeneration of the phenomenon manifests in two scenarios: a) flow of the fluid, which is highly dispersing like a non-dense gas and b) flow of fluid far away from the source of flow, when the velocity of the flow is incomparably smaller than the gradient of the density. First, we will show that both types of flows can be modeled using the Einstein paradigm. We will investigate the question: What features will particle flow exhibit if the time interval of the free jump is inverse proportional to the density and its gradient ? We will show that in this scenario, the flow exhibits localization property, namely: if at some moment of time t₀ in the region, the gradient of the density or density itself is equal to zero, then for some T during time interval [t₀, t₀ + T] there is no flow in the region. This directly links to Barenblatt's finite speed of propagation property for the degenerate equation. The method of the proof is very different from Barenblatt's method and based on the application of Ladyzhenskaya - De Giorgi iterative scheme and Vespri - Tedeev technique. From PDE point of view it assumed that solution exists in appropriate Sobolev type of space.

• The Korean Journal of Mycology
• /
• v.32 no.1
• /
• pp.39-44
• /
• 2004

Gastrodia elata has been cultivated using mycorrizal fungi including Armillaria mellea as an energy source (myco-hetrotrophy) because it is aphyllous and achlorophyllous archid. But the yields of G. elata have been recently decreased owing to the degeneration of spawn tuber arised from successive asexual reproduction. Therefore, this study was carried out to solve this degeneration by seed germination, namely sexual reproduction. The seed germination of G. elata was excellent on the fallen leaves medium of oak tree. The fruetification rate of the capsule of G. elata by artificial pollination was 94.1% and better than natural pollination. The living weight of capsules of G. elata suitable for seeds germination was above 31 mg. The middle-matured seeds and matured seeds of capules were largely germinated, while the immatured seeds was small germinated. The storage temperature of the cropped capsules suitable for a favorable seed germination was $0{\sim}5^{\circ}C$ and the storage period of it was one month.

• Journal of the Institute of Electronics Engineers of Korea SD
• /
• v.49 no.3
• /
• pp.15-20
• /
• 2012

This paper presents a 5-Gb/s optical receiver circuit realized in a $0.13-{\mu}m$ CMOS technologies for the applications of high-speed digital interface. Exploiting modified RGC input stage at the front-end transimpedance amplifier, interleaving active feedback and source degeneration techniques at the limiting amplifier, the proposed optical receiver chip demonstrates the measured results of $72-dB{\Omega}$ transimpedance gain, 4.7-GHz bandwidth, and $400-mV_{pp}$differential output voltage swings up to the data rate of 5-Gb/s. Also, the chip dissipates 66mW in total from a single 1.2-V supply, and occupies the area of $1.6{\times}0.8mm^2$.

• Applied Microscopy
• /
• v.22 no.2
• /
• pp.97-117
• /
• 1992

In order to study process of spermiogenesis of the Korean greater horseshoe bat, Rhinolophus ferrumequinum korai, the cycle of seminiferous epithelium was examined by the light and electron microscope and the following results were obtained based on the epithelial cell differentiation. 1. Spermiogenesis occurred from early July to mid-Octber, and spermatogenic activity was vigorous from mid-August to late September. Spermatocytes including spermatogonia were found to be degenerated in only July. It is deduced that the degeneration serves as the mechanism to regulate effective use of energy to prepare for mating and hibernating periods, and regulation of breeding cycle. 2. Spermiogenesis of the Korean greater horseshoe bat was divided according to differentiation of the cell structure, into Golgi, cap, acrosome, maturation and spermiation phases; Golgi, cap and spermiation phases were further divided into two steps of early and late phase respectively, and acrosome phase into three steps of early, mid and late phases, and maturation phase has only one step. Hence, the spermiogenesis consists of ten phases. The first research was done in this article on the changes of chromatin with nucleus, the time of appearance and disappearance of chromatin granules, in case of Korean greater horseshoe bat (Rhinolophus ferrumequinum korai). Chromatin granule began to be condensed in late Golgi and the condensation proceeded to form an irregular mass of a electron-dense chromatin in a form of circular cylinder in the center of nucleus at the phase of maturation. Finally, the chromatin condensation proceeded and perfect nucleus of sperm with homogeneous density was formed when the sperm was separated from Sertoli cell. Therefore, appearance and disappearance of chromatin granules occurred in the period of time between late Golgi and maturation phase, The tail of sperm began to develop in early cap phase, Numerous lipid droplets were obseved in the cytoplasm of spermatids during the maturation phase, which seemed to be used as energy source necessary to make mature sperm during spermiogenesis.

• The Korean Journal of Physiology and Pharmacology
• /
• v.13 no.5
• /
• pp.373-378
• /
• 2009

Cerebellar Purkinje cells (PCs) play a crucial role in motor functions and their progressive degeneration is closely associated with spinocerebellar ataxias. Although immunohistochemical (IHC) analysis can provide a valuable tool for understanding the pathophysiology of PC disorders, the method validation of IHC analysis with cerebellar tissue specimens is unclear. Here we present an optimized and validated IHC method using antibodies to calbindin D28k, a specific PC marker in the cerebellum. To achieve the desired sensitivity, specificity, and reproducibility, we modified IHC analysis procedures for cerebellar tissues. We found that the sensitivity of staining varies depending on the commercial source of primary antibody. In addition, we showed that a biotin-free signal amplification method using a horseradish peroxidase polymer-conjugated secondary antibody increases both the sensitivity and specificity of ICH analysis. Furthermore, we demonstrated that dye filtration using a $0.22\;{\mu}m$ filter eliminates or minimizes nonspecific staining while preserving the analytical sensitivity. These results suggest that our protocol can be adapted for future investigations aiming to understand the pathophysiology of cerebellar PC disorders and to evaluate the efficacy of therapeutic strategies for treating' these diseases.