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Vitis amurensis Ruprecht root inhibited ${\alpha}$-melanocyte stimulating hormone-induced melanogenesis in B16F10 cells

  • Jin, Kyong-Suk;Oh, You Na;Hyun, Sook Kyung;Kwon, Hyun Ju;Kim, Byung Woo
    • Nutrition Research and Practice
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    • v.8 no.5
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    • pp.509-515
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    • 2014
  • BACKGROUND/OBJECTIVES: The root of Vitis amurensis Ruprecht, a sort of wild-growing grape, has been used in oriental medicine for treatment of skin ailments; however, its dermatological activity is not sufficiently understood. The aim of this study was to investigate tyrosinase inhibitory and anti-melanogenic activities of V. amurensis Ruprecht root methanol extract (VARM) in B16F10 mouse melanoma cells and to attempt to isolate and identify the active compound issued from VARM. MATERIALS/METHODS: Anti-melanogenic activity of VARM was analyzed in ${\alpha}$-melanocyte stimulating hormone (MSH)-stimulated B16F10 cells through evaluation of antioxidative activity as well as inhibited tyrosinase activity and melanin contents compared with those of kojic acid and arbutin. After anti-melanogenic analysis of VARM, serial fractionation, nuclear magnetic resonance (NMR), and thin layer chromatorgraphy (TLC) were applied for identification of active compounds contained in VARM. RESULTS: VARM significantly inhibited oxidative stress and tyrosinase activity and attenuated ${\alpha}$-MSH-induced melanin production in B16F10 cells. For isolation of active compounds, VARM was fractionated using a series of organic solvents, including dichloromethane ($CH_2Cl_2$), ethyl acetate (EtOAc), and n-butanol (n-BuOH). Among fractions showing anti-melanogenic activity, the CH2Cl2 fraction induced the most potent attenuation of melanogenesis without cytotoxicity and the major compound in the $CH_2Cl_2$ fraction was identified as betulinic acid. Betulinic acid isolated from the $CH_2Cl_2$ fraction of VARM significantly attenuated ${\alpha}$-MSH-induced melanogenesis in a dose dependent manner, which was stronger than that of arbutin used as a positive control. CONCLUSIONS: These results indicate that VARM inhibits oxidative stress, tyrosinase activity, and ${\alpha}$-MSH-induced melanogenesis in B16F10 cells, due primarily to the active compound, betulinic acid, in the $CH_2Cl_2$ fraction.

A Qualitative Analysis on the Characteristics of "Best Practice" in Mathematics (수학과 좋은 수업 사례에 대한 질적 분석)

  • Lee, Dae-Hyun;Choe, Seung-Hyun
    • Journal of the Korean School Mathematics Society
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    • v.9 no.3
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    • pp.249-263
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    • 2006
  • The purpose of this study is to investigate the characteristics of 'best practire' in mathematics and suggest some solutions to several problems emerging in mathematics classes of secondary schools. The study was carried out by using qualitative research methods such as class observations and in-depth interviews with six teachers. Based on the collected data, we could sort out the major patterns which characterize 'the good mathematics teaching' at schools in Korea. The common characteristics of best practice in mathematics are drawn out from the six cases. The common characteristics include revising the curriculum and text books, realistic mathematics education, using ICT and meta-cognition, introduction with motivation and interest, performance assessment and managing differentiated small group. Results implied that six teachers used a variety of instructional methods and strategies which is related with the common characteristics of good mathematics teaching. Also these teachers not only improved their own classroom practices but also participated in various professional community of mathematics education and shared their practical knowledge. In conclusion assorted efforts from the government and the school principals as well as the teachers are prerequisite for practicing and spreading good mathematics teaching across the classrooms.

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A Study on Health Administration Status and Medicare Insurance Program in Universities and Colleges in Korea (우리나라 대학(大學)에 있어서의 학생보건관리(學生保健管理) 및 의료보험제도(醫療保險制度)에 관(關)한 실태(實態) 조사(調査) 연구(硏究))

  • Choi, Sam-Sup;Kang, Ji-Yong;Koo, Youn-Choul
    • Journal of Preventive Medicine and Public Health
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    • v.5 no.1
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    • pp.125-132
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    • 1972
  • The status of health administration and medicare insurance program of 58 universities and colleges of 4-year course was studyed in 1971 and the following results were obtained; 1. The average number of students of 30 universities was $4,800{\pm}2,600$ and that of 36 colleges was $780{\pm}620$. 2. The types of health service facilities for the students varied widely according to the institutions, from an elaborate one, university health center, to a poor one, first aid room. 3. Thirty-six out of 58 institutions had some sort of health service facilities, either health center or health service room. And 14 out of 36 institutions had elaborate health service facilities such as university health center or student health center 4. The number of full time staff of the health center and that of the health service room were 2 to more than 10 and 0 to 3 respectively. 5. The range of student health service fee varied widely according to the institutions from \50 to \550 per student, per semester. The average cost of student health service fee at the institutions with health centers was $\300{\pm}150$ and that with health service rooms was $\200{\pm}150$ per student, per semester. 6. Utility rate of the student health service facilities at the institutions with health centers and with health service rooms were 1,200 to 1,400 and 3,900 to 4,100 per 1,000 students per year. 7. There was an obvious increasing tendency of tuberculosis prevalence rate in the students. 8. The institutions which had appointed hospitals for student medicare were 24; where the reduction rate of medical expenses for students varied from 10 to 50 per cent. 9. Students medicare insurance program was adopted by six universities which accomodated more than 2,000 students. 10. The range of student medicare insurance fee varied widely according to the institutions from \140 to \800 per student per year. Each of the six universities which had adopted the insurance program had each own's special regulations to apply for pay claims.

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A study on factors related to satisfaction level with dental services (치과 의료서비스 만족도 관련요인 연구)

  • Go, Eun-Jeong;Lee, Yong-Hwan;Heo, Seung-Ju
    • Journal of Korean society of Dental Hygiene
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    • v.10 no.2
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    • pp.393-411
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    • 2010
  • Objectives : The purpose of this study was to examine factors related to the satisfaction level of patients with dental services. Methods : The subjects in this study were 200 patients at three different dental clinics in South Gyeongsang Province, on whom a survey was conducted from June 1 to July 31, 2009. The collected data were analyzed, and the findings of the study were as follows. Results : In regard to the general characteristics of the patients investigated, the women(61.5%) out-numbered the men. By age, those who were at the age of 30 and down made up the largest age group(47.0%). By academic background, the greatest group received junior-college education(54.0%). By occupation, the company employees constituted the largest group (50.5%). By income, the greatest group earned an income of 2 to 2.99 million won(75.0%). Second, as to connections between the characteristics of dental treatment and the reason of choosing the dental clinics, the largest group(70.4%) chose the dental clinics by word of mouth or the recommendation of others. Concerning the reason of dissatisfaction, the biggest group(72.7%) was unsatisfied with medical costs. As for the degree of explicit complaint, the greatest group(49.7%) sort of complained about what made them dissatisfied. Regarding the type of treatment, the biggest group(49.0%) received prosthodontic treatment. In relation to fear for dental treatment, the largest group(34.0%) feared receiving the treatment, and the biggest group(42.6%) did that due to the sound of machines. Third, satisfaction with services(0.762) had the closest correlation to overall satisfaction level, followed by satisfaction with employees(0.735), satisfaction with dentists(0.644) and satisfaction with convenient facilities (0.579). Fourth, the factors that affected overall satisfaction level were gender, the reason of choosing the dental clinic, satisfaction with dentists, satisfaction with employees, satisfaction with services and satisfaction with convenient facilities. The patients who were better satisfied with services(p<0.001), who were more contented with dentists(p<0.001), who chose the dental clinics by the recommendation of others(p<0.01), who were male(p<0.05), who were more gratified with employees(p<0.05) and who were more contented with convenient facilities expressed better overall satisfaction. Conclusions : The above-mentioned findings suggested that dental institutions should keep track of the expectations of patients to prepare differentiated strategies for marketing and human-resources development in consideration of their own circumstances to boost the satisfaction level of patients. Specifically, it's required to heighten the satisfaction level of patients with dentists.

Characteristics of the Decontamination by the Melting of Aluminum Waste (용융에 의한 알루미늄 폐기물의 제염 특성)

  • Song Pyung-Seob;Choi Wang-Kyu;Min Byung-Youn;Kim Hak-I;Jung Chong-Hun;Oh Won-Zin
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.3 no.2
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    • pp.95-104
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    • 2005
  • Effects of the aluminum melting temperature, melting time and a kind of flux agents on the distribution of surrogate nuclide were investigated in the electric furnace at the aluminum melting including surrogate radionuclides(Co, Cs, Sr) in order to establish the fundamental research of the melting technology for the metallic wastes from the decommissioning of the TRIGA research reactor. It was verified that the fluidity of aluminum melt was increased by adding flux agent but it was slightly varied according to the sort of flux agents. The results of the XRD analysis showed that the surrogate nuclides move into the slag phase and then they were combined with aluminum oxide to form more stable compound. The weight of the slag generated from aluminum melting test increased with increasing melting temperature and melting time and the increase rate of the slag depended on the kind of flux agents added in the aluminum waste. The concentration of the cobalt in the ingot phase decreased with increasing reaction temperature but it increased in the slag phase up to 90$\%$according to the experimental conditions. The volatile nuclides such as Cs and Sr considerably transferred from the ingot phase to the slag and dust phase.

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A study on the application of redundancy in information design (정보디자인의 잉여성적용 연구)

  • Oh, Byung-Keun;Hong, Suk-Il
    • Archives of design research
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    • v.18 no.1 s.59
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    • pp.49-58
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    • 2005
  • Focusing on the information sender, message, channel, and receiver, the existing information theory deals with the noise, information contents, and probability of choice, which involve in the process of information transmission. In the current digital environment, besides simply conveying information itself through media, the important issue is how to efficiently convey information. Therefore, we need to analyze the theory in different perspective, and to research the Information redundancy for the methodology of information design. The redundancy causes the receiver to have attraction to the information and to reduce its contents due to its supplement and repetition. So it can play a role of efficient communication method. The concept of redundancy is applied to the communication of art such as literature, architecture, painting, and design to accomplish efficient communication. In order to get persuasive information design for the receiver's perspective we need to make use of this concept. The redundancy can be applied with the technical aspect of multimedia and Interaction, which add supplemental expression, or sort of event for the receiver's experience and memory. In the process of constructing information it can be applied with the structure of gaming redundancy, entropy, the accumulating communication code using entertaining feature, and storytelling methodology. The noise and entropy could be used for the means of making redundancy, not the obstacle to information. The redundancy gives the receiver attraction on the information and makes them have strong will of interpreting it so that the purpose of conveying information will be accomplished efficiently.

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A Study on the Art Make-Up Based on Surrealism -Focus on the creation of body painting works- (초현실주의 Art Make-up에 관한 연구 -Body Painting 작품제작을 중심으로-)

  • 김순구
    • Archives of design research
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    • v.15 no.4
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    • pp.47-56
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    • 2002
  • The purpose of this study is to clarify how modern formative art reflects surrealism and propose a Art Make-up expressed based on the trend. The study also aims at demonstrating the part that the Make-up based on surrealism is also an artistic being keeping pace with the trend of art. For the purpose, the study reviewed related theoretical backgrounds and works as manufactured. Based on these theoretical backgrounds, the author anufactured works of his own, realizing the combination between theoretical and practical aspects. Those works were motivated from existing surrealistic paintings or otherwise made surrealistically through creation by the author for himself. The works included , (Work 2-MAGRITTE's Expression>, , , and . Results of the study, which were obtained through manufacturing the works, can be described as follows. First, senses of modern formation and line were emerged by applying the materials and images frequently shown in surrealistic paintings to human bodies. Second, characteristics of the Art Make-up for artistic image were revealed, not limited to colors, expressions, subjects, techniques and materials. Third, an unlimited, interesting expression of the Make-up using a variety of materials became possible by applying extremely subjective, surrealistic paintings and images in various ways. This paper proposed that the Make-up should be a artistic image itself, not as a secondary means for an obvious expression of personal beauty which has been maintained since the ancient times. The researcher tried to put the Art Make-up into our daily life by making creative works which viewed and psychologically enjoyed by people and by proposing the works as sort of image theme to the spectators.

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Exploratory Study on the Possibilities of Convergence with Music in Writing Classes (글쓰기 수업에서 음악과의 융합 가능성에 대한 탐색적 연구)

  • Lee, Ran
    • The Journal of the Korea Contents Association
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    • v.20 no.8
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    • pp.88-100
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    • 2020
  • This is an exploratory study based on the literature reviews which examined the possibilities and necessities of multimodal writing curriculum for liberal education. The purpose of this study is to analyze the existing research results which utilized the teaching methods associating music and writing, and to find the educational implications, and finally in terms of writing education, to suggest the possibilities of writing classes' convergent forms with music extracted from the results of the existing studies. Those studies were categorized to four patterns: WAC, effects of therapy, materials for writing, and new literacy. Based on Meyrowitz's perspective, firstly music can be utilized as a circumstance, which means a teacher can indirectly take the emotional, reminding, and healing effects of background musics. Secondly, music can play an important role of materials in thinking and writing, which is the most generally utilized pattern today. The effects are found in all of affective, cognitive, and strategic domains by utilizing music as a sort of reading materials. Thirdly, the convergent writing of music and narrative is suggested. Music is an independent language that can interact with narrative and construct text meanings in this kind of writing classes. These three dimensions of convergence have different perspectives, but sometimes occur at a same time or as a connected pattern. This study proposes that writing teachers need to improve their competence in music as well and to have professional concerns and efforts to develop their convergent writing teaching skills with music for these classes. Finally, this study stresses that team teaching can be an alternative for them.

Investigating Secondary Mathematics Teachers' Capacity to Select and Pose Cognitively Demanding Tasks (중등 수학교사의 과제 이해 및 변형 능력 : 인지적 노력 수준 중심으로)

  • Kim, Jung Eun;Lee, Soo Jin;Kim, Ji Soo
    • School Mathematics
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    • v.17 no.4
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    • pp.633-652
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    • 2015
  • The purpose of the present study is twofold: one is to understand secondary mathematics teachers' capacity to sort out given tasks based on Stein & Smith(1998)'s Cognitive Demands of Mathematical Task Framework; the second is to examine how the teachers assess the levels of cognitive demand indicated in students' reponses and how they modify the tasks to elicit the students' higher levels of cognitive activity. The analysis of 45 teachers' responses to the survey indicates that the teachers, in general, could select appropriate tasks for the given goal of the lessons but some made the decision merely by their appearances. Even though the teachers chose a particular level with different reasons amongst each other, most teachers could correctly evaluate the levels of cognitive demand of the students' responses. Finally, teachers could pose cognitively demanding tasks using various methods, but a number of them felt challenged in creating word problems that were realistic and aligned with curriculum.

Effects of Superimposition Cue and Selective Attention on the Formation of Subjective Contours (중첩단서 및 선택적 주의가 주관적 윤곽의 발생에 미치는 효과)

  • 정재훈;정찬섭
    • Korean Journal of Cognitive Science
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    • v.2 no.2
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    • pp.261-278
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    • 1990
  • Two experiments were performed to test whether the perception of subjective contours occurs not only through bottom-up processes but also throught top-down processes by manipilating such cognitive factors as superimposition cues and selective attention.In the first experiment,the phenomenon of neon color spreading effect was used to investigate the effect of superimpoed Kanizsa triangles.To identify the effect of superimposition, two different colors were used,one for each overlapped Kanizsa triangle,and the subjects were asked which neon-like color they saw for the overlapped portion.Result indicates that the only neon-like color of the superimposing triangle can be perceived,which implies that super impostition cues may act upon the formation process of subjective contour as a a sort of top-down information.In the second experiment,two partially ovwelapped Kanizsa squares of which direction of superimposition were ambiguous and thus reversable depending on the observer's mental set were used to inverstigate the effect of selective attention.Subjects were instructed to pay attention only to one of the squares and report the neon- like color of the overlapped portion of them.Result shows that the subjects perceive only the neon-like color of superimposing square,which implies that such cognitive factors as selective attention may strongly influence the formation of subjective contours. In conclusion,the results of this study show that the subective contour does not depend only on bottom-up processes but also on such cognitive factors as super-imposition cues and selective attention.