• Title/Summary/Keyword: solid medium

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Output Characteristics of the Longitudinally Pumped 946 nm Nd:YAG Laser with Laser Diode (반도체 레이저로 종펌핑하를 946 nm Nd:YAG 레이저의 출력 특성)

  • Park, Cha-Gon;Choo, Han-Tae;Kim, Gyu-Ug
    • Korean Journal of Optics and Photonics
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    • v.18 no.4
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    • pp.270-273
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    • 2007
  • We have investigated the output characteristics of the 946 nm Nd:YAG laser which is longitudinally pumped by a fiber coupled laser diode. The temperature of a Nd:YAG crystal mount was kept constant by a controller with thermoelectric cooler. As a result, we measured more intense output at a low temperature, and then the maximum output power was measured to be 870 mW when the pumping power and the temperature were 9.95 W and $5^{\circ}C$, respectively. It appeared that output was decreased above 10 W pump power because of the thermal effects in gain medium.

Selection of Active Grow Hairy Root Lines in Ginseng (고생장 인삼 모상근의 선발)

  • 양덕춘;김용해;양덕조;민병훈;신성련;최광태
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.6
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    • pp.525-530
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    • 1998
  • These studies were carried out to select the active grow hairy root lines induced from various ginseng(Panax ginseng C. A. Meyer) parts. Hairy roots were induced in root explants, stem and petiole in vitro by A. rhizogenes R1000 or A. rhizogenes $A_4$. These hairy roots could be grown on the phytohormone free medium, and PCR analysis of rol C and vir C gene fragments confirmed that hairy roots were transgenic tissues. We have selected 11 hairy root lines with active growing characters among 300 hairy root lines selected based on growth and morphological characteristics on 1/2MS solid media with 250 mg/L carbenicillin. Morphological characteristics of selected 11 hairy root lines were thickness and thiness of main roots, and many projection for lateral roots, active grow of lateral roots. Among selected 11 hair root lines prominent characteristics of hairy roots with active growing characters were thiness of main roots and active grow of lateral roots. But characteristics of low growing hairy roots were thickness of main roots and low grow of lateral roots. Finally we have selected actively growing hairy roots, KGHR-1, KGHR-5, KGHR-8 among 11 hairy root lines.

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Effect of Cytokinins on Nodal Cultures of Citrus Species (감귤류의 마디배양에서 싸이토키닌류가 기내 대량증식에 미치는 영향)

  • Kyung chul HAN;Youn Hwa HAN
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.3
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    • pp.145-150
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    • 1994
  • This study was conducted to determine the effects of 3 cytokinins (BA,2iP and kinetin) and their concentrations (0, 0.1, 0.5, 1.0, and 2.0 mg/L) on multiple shoot production of Citrus spp. 'Sambokam' and 'Byungkyool' by nodal culture. Nodal explants were obtained from in vitro germinated seedlings of both cultivars. 'Sambokam' produced more multiple shoots than did 'Byungkyool' by nodal culture. Among the 3 cytokinins tested in this study BA supplemented in semi-solid MS basal medium was the most effective stimulator for multiple shoot production, and an optimal concentration was determined to be 1.0 mg/L. Shoot elongation and root formation were inhibited by increasing cytokinin concentration, regardless of cytokinin types. BA at 1.0 mg/L produced the most multiple shoots and the highest number of leaves in 'Sambokam', whereas any cytokinin and concentration studied in this experiment did not affect any scored variables such as shoot and leaf numbers, etc. in 'Byungkyool'.

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Plant Regeneration and Somatic Embryo Formation from Root-Derived Callus of Rice (벼 뿌리조직 유래의 캘러스로부터 체세포배 형성과 식물체 재분화)

  • 손재근;김경민;김종수
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.3
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    • pp.143-148
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    • 1995
  • The competence of callus formation and plant regeneration from root derived callus was higher in japonica cultivars than those of Tongil-type cultivars of rice. A japonica type cultivars Yeongdeogbyeo, showed the highest capacity (13%) for plant regeneration from root calli of 6 cultivars tested. The callus induced from seed and root tissues maintained higher capacity for plant regeneration during 7 passages of subculture on N$_{6}$ solid media at 2-week intervals. The maximum frequency (2 x 10$^{5}$ mL) of round cells and their cell colonies showed about 24 days after suspension culture of root-derived callus in N$_{6}$ medium with lmg/L 2,4-D, 300mg/L casein hydrolysate, 10mM L-proline, 20g/L sucrose and 30g/L sorbitol. The frequency of somatic embryo formation in suspension cultures of root-derived callus increased with prolonged advance of subculture time from 30 to 90 days, but their regenerative capacities decreased.

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COLLOIDAL PROPERTIES OF HOLLOW LATICES AND THEIR ROLES IN CONTROLLING COLORIMETRIC PARAMETERS OF COATED PAPER SURFACE

  • Hitomi HAMADA;Yoko SAITO
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 1999.04b
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    • pp.309-314
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    • 1999
  • With a view to seek the influence of hollow sphere pigments of latex upon the printed color on coated paper surface, the hollow sphere pigments were compared with filled ones in a variety of experimental approaches. Colloidal properties of latices were determined by measuring zeta potential and particle size distribution. For the amphoteric filled sphere pigment of latex, the polarity was reversed from the negative side to the positive side with decreasing pH. An extraordinarily high peak in the particle size distribution of the amphoteric filled evidenced aggregation between latex particles near the isoelectric point, depending on the electrolyte concentration and pH of the suspending medium. Coated papers containing the hollow sphere pigment in their coating improved optical properties like gloss and brightness. Optical parameters solely of the coating could account for this finding. An equation derived from the Kubelka-Munk equation calculated them fro twice measurements of reflectance of a coated paper over two substrates of different reflectances. This method permitted to predict brightness of coated paper of which coat weight would be different fro the actual one. The colorimetric parameters of solid-printed surfaces of the coated papers closely related to optical and structural properties of the coated papers. The color of the printed surfaces was dominated by the brightness and the smoothness of the coated papers. The hollow sphere pigments were proved to improve optical properties of coated paper and to control minutely colorimetric parameters of printed surfaces.

Microfiltration of Chlorella sp.: Influence of material and membrane pore size

  • Ahmad, A.L.;Yasin, N.H. Mat;Derek, C.J.C.;Lim, J.K.
    • Membrane and Water Treatment
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    • v.4 no.2
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    • pp.143-155
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    • 2013
  • Four membranes were used to separate Chlorella sp. from their culture medium in cross-flow microfiltration (MF) experiments: cellulose acetate (CA), cellulose nitrate (CN), polypropylene (PP) and polyvinylidenefluoride (PVDF). It was found that the hydrophilic CA and CN membranes with a pore size of 1.2 ${\mu}m$ exhibited the best performances among all the membranes in terms of permeation flux. The hydrophobicity of each membrane material was determined by measuring the angle between the water (liquid) and membrane (solid). Contact angle measurements showed that deionized (DI) water had almost adsorbed onto the surfaces of the CA and CN membranes, which gave $0.00^{\circ}$ contact angle values. The PP and PVDF membranes were more hydrophobic, giving contact angle values of $95.97^{\circ}$ and $126.63^{\circ}$, respectively. Although the pure water flux increased with increasing pore diameter (0.8 < 1.2 < 3.0 ${\mu}m$) in hydrophilic CA and CN membranes, the best performance in term of filtration rate for filtering a microalgae suspension was attained by membranes with a pore size of 1.2 ${\mu}m$. The fouled membrane pore sizes and pore blocking were inspected using a scanning electron microscope (SEM). MF with large pore diameters was more sensitive to fouling that contributed to intermediate blocking, where the size of the membrane pores is almost equivalent to that of cells.

Outer Shape Design of Rotating Medium Antenna Installed on Ship Mast (함정 마스트 장착 레이더용 회전 중형 안테나의 외부 형상 설계)

  • Cho, Ki-Dae;Kim, Ki-Wan;Kwon, Min-Sang;Hong, Seong-Il
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.36 no.8
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    • pp.843-850
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    • 2012
  • To satisfy the operational requirements of surveillance radar and the operational concept of a ship combat system, an active-array-type antenna was designed using solid-state transmitters and receivers. The arrangement of the constituent electronic components of the antenna system, considerably large in size, and therefore, the antenna needs to be housed in a box. The rotational antenna system installed on a ship mast is installed in a sealed housing to protect the interior equipment from environmental conditions. The outer shape of the housing is that the antenna should be capable of rotating normally despite strong winds. Furthermore, in recent times, designs with low radar cross section (RCS) have attracted attention from the viewpoint of improving the stealth of the ship. In this light, four types of models are proposed for the outer shape design of the antenna system, and they are investigated from the viewpoint of low RCS design as well as wind load.

Construction of the Phosphate-Limitation Inducible Expression Vector Containing the phoA Promoter of Enterobacter aerogenes (Enterobacter aerogenes 의 phoA 유전자 Promoter를 이용한 인 제한환경에서 발현하는 벡터 구축)

  • 장화형;고병훈;박신영;이성호;김성진;임유정;한갑진;김영호;이영근
    • Korean Journal of Microbiology
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    • v.38 no.4
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    • pp.318-321
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    • 2002
  • To induce recombinant protein under phosphate restricted conditions such as soil, we have constructed the expression vector (pEAAP) with phoA gene promoter of Enterobacter aerogenes. To construct the pEAAP, deletion of the T7 promoter and lac operator from pET-22b(+) by BglII-XhoI digestion and addition of the phoA gene promoter (containing the pho box) were performed. To test pEAAP as an expression vector controled by phosphate limitation, pEAPHY1 was constructed with the phytate gene (Bsa-phy1) of Bacillus subtillis var. amyloliquefaciens (KCTC 8913P). Under the phosphate-limitation condition, CK-PHY1 ( Escherichia coli JM109 was transformed with pEAPHY1) expressed the 41 kD Bsa-Phy1 . Also CK-PHY1 formed the clear zone in solid medium containing phytate as a sole phosphate source.

Enzymatic Decolorization of Various Dyes by Trametes versicolor KCTC 16781 (Trametes versicolor KCTC 16781에 의한 다양한 염료의 색도제거 특성)

  • 박철환;이유리;김탁현;이명구;이병환;이진원;김상용
    • KSBB Journal
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    • v.18 no.5
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    • pp.398-403
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    • 2003
  • Due to the low biodegradability of dyes, conventional biological wastewater treatment systems are inefficient in treating dye wastewater. Various white-rot fungi were investigated for the decolorization of six industrial dyes (reactive blue 5, reactive blue 16, reactive black 5, acid black 52, reactive orange 16, and acid violet 43). Among ten fungi, T. versicolor KCTC 16781 was selected as a testing strain because this had the best performance of decolorization for six dyes from the results of the solid culture experiments. In liquid culture medium, T. versicolor KCTC 16781 decolorized over 96% of six dyes for 48 hrs. Laccase started to produce in the early stage of the culture, and showed the highest peak value of 2.3 U/mL in 24 hrs. Enzyme activity remained constant until the end of culture. Fungal decolorization is a promising alternative to replace or supplement present treatment process.

Synthesis of Nano Size $BaCeO_3$ as an Effective Flux Pining Center for YBCO Superconductor (YBCO 초전도체의 효과적인 플럭스 피닝 센터로서의 나노 크기 $BaCeO_3$ 합성)

  • Youn, J.S.;No, K.S.;Kim, Y.H.;Jun, B.H.;Lee, J.P.;Jung, S.Y.;Kim, C.J.
    • Progress in Superconductivity
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    • v.10 no.1
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    • pp.12-16
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    • 2008
  • In this work, nano size $BaCeO_3$, which is a possible flux pinning medium of melt processed $YBa_{2}Cu_{3}O_x$ superconductor, was synthesized by the conventional solid state reaction method using powders. $BaCeO_3$ and $CeO_2$ were mixed thoroughly using a ball milling for 24 hours and calcined at $1200^{\circ}C$ for 5 hours for the formation $BaCeO_3$ powder. The obtained $BaCeO_3$ powder was attrition milled at various milling times of 60 min, 120 min and 240 min. The $BaCeO_3$ powders of various milling times were mixed with $YBa_{2}Cu_{3}O_x$ powder. Seed melt processed $YBa_{2}Cu_{3}O_x$-$BaCeO_3$ (15wt.%) superconductors were prepared and the superconducting properties were investigated. It was found that $T_c$ of $Y_{1.5}Ba_{2}Cu_{3}O_x$ samples was not significantly affected by $BaCeO_3$ addition, but $J_c$ of samples was increased by $BaCeO_3$ addition. The $J_c$ improvement by fine $BaCeO_3$ powder (120 min attrition-milled) was effective at low magnetic fields less than 2 T.

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