• Journal of Environmental Science International
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• v.21 no.2
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• pp.253-261
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• 2012

We isolated and characterized novel duck feather-degrading bacteria producing keratinase. Twelve strains were isolated from soil and faces at poultry farm, and decayed feathers. They were identified as Bacillus methylotrophicus, Pseudomonas geniculata, Pseudomonas hibiscicola, Exiquobacterium profundum, Bacillus pumilus, Bacillus amyloliquefaciens, Chryseobacterium indologenes, Bacillus thuringiensis, Thermomonas koreensis, respectively, by phenotypic characters and 16S rRNA gene analysis. Generally, the level of keratinase production was not proportional to feather degradation rate. The highest keratinolytic activity was observed in the culture inoculated with Chryseobacterium indologenes D27. Although all strains did not degrade human hair, strains tested effectively degraded chicken feather(53.8-91.4%), wool(40.4-93.0%) and human nail (51.0-82.9%). These results suggest that strains isolated could be not only used to improve the nutritional value of recalcitrant feather waste but also is a potential candidate for biotechnological processes of keratin hydrolysis.

• Journal of Korean Society of Forest Science
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• v.95 no.2
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• pp.155-159
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• 2006

An efficient method for in vitro propagation of the medicinal plant Hovenia duleis, was established. Plantlets for micropropagation of H. dulcis were obtained from in vitro germinated seeds. The effectiveness of various levels of cytokinins (BAP, Kinetin and TDZ) on multiple shoot formation from stem nodes was tested. BAP (1.0 mg/L) treatment induced highest number of multiple shoots. The growth pattern of plantlet on various culture media was undertaken. The shoot elongation was optimal on 2MS basal medium without growth regulators. The in vitro rooting ability of H. dulcis shoots was examined with two-auxins IAA and IBA. The IAA (1.0 mg/L) treatments induced earliest rooting with maximum number of roots and root growth. Rooted shoots were transferred directly to small pots with artificial soil and such established plant exhibited a normal growth pattern similar to wild plantlet.

• Proceedings of the Korean Geotechical Society Conference
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• 1987.06a
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• pp.51-80
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• 1987

In this studys a numerical analysis on the defomation of foundation layer was carried out by indroducing joint element. The method using the joust element between adj assent different materials has been originally developed for rock behavior(Goodman, et al. 1968) . The application of this method to the interface between the footing and soil layer proved satisfactory(Ghaboussi p et at. 1973). Authors tried to obtain the deformation of rrcompound foundation layerg", which vertically or horizontally or both consists of the natural(or intact) soft clay layer and the layer improved artificially in order to get high stiff-fness with replacement or chemical treatment to reduce the excessively detrimental settlemellt or lateral displacement in case of banking or building the civil structure on the soft layer. The joint conditions were classified into three categories : contacts sliding and separation. By coupling "JOINT" as a subroutine into multi-purpose code for the finite element method of the foundatlion daveloped by authors on the assumption that shearing and normal displacement can not be coupledl which terms pinon-dilatant" and by selecting modified Cam-clay modeIP the deformation analysis was performmed. The results using joint element were compared with those secured without introduction of joint element Nain results analized are as follows : 1. For the prediction of settlement and lateral desplacement, the result due to joint element was evaluated larger, which was regarded safe. 2. For the determination of ultimate bearing capacetyi the value using joint element appeared smaller by 20%, which was also safe.

• Journal of the Korean Geotechnical Society
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• v.19 no.6
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• pp.107-114
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• 2003

Among soft ground treatment methods with granular soil used in domestic, the sand compaction pile method has been utilized greatly, but, as a result of exhaustion of sand and increase of unit cost, the necessity of an alternative method is suggested. In this study, the static load tests for crushed-stone compaction piles which were constructed on test field were performed. Based on test results, stress concentration ratios between the crushed-stone compaction pile and the soft ground were investigated and estimated. At loading pressure, settlement showed decreasing tendency as replacement rate increases. At replacement rate of 20%, yield pressure was smaller but, at replacement rates of 30% and 40%, settlement and yield pressure were similar. The stress concentration ratio was within the range of 1.7 to 3.0 and it was higher as replacement rate increased.

• Proceedings of the Korean Geotechical Society Conference
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• 2005.03a
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• pp.705-712
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• 2005

Among soft ground treatment methods with granular soil used in domestic, the sand compaction pile method has been utilized greatly, but, as a result of exhaustion of sand and increase of unit cost, a necessity of an alternative method is suggested. In this study, the static load tests for group crushed-stone compaction piles which were constructed at in-situ site were performed. Pile diameter was 700mm and area of loading plates were changed. The static load tests of single and group piles were performed for area replacement ratio of 20, 30 and 40%. Based on test results, bearing capacity of group crushed-stone compaction pile were estimated. The more both single pile and group pile increase, the more yield bearing capacity tended to increase. Also, the yield bearing capacity of a group pile is about 50% less than the yield bearing capacity of a single pile. If the ground reinforced with the crushed-stone compaction pile is replacement ratio of $20{\sim}40%$, RIYB of both single pile and group pile increases qualitative tendency of linear more than original ground

• Proceedings of the Korean Geotechical Society Conference
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• 2004.03b
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• pp.684-691
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• 2004

Various researches have been undertaken to develope a method of enhancing consolidation. This study investigated the effect of ultrasound on consolidation through a series of laboratory experiments. The tests were conducted using a specially designed and fabricated equipments which can apply ultrasonic energy on a soil sample at consolidation test. Specimens were prepared using a centrifuge facility, and test conditions included ultrasonic power and treatment time. The results of the study show that the effect of ultrasonic energy on consolidation is significant. The degree of significance varies with the test conditions. It could be concluded that the study showed potential application of ultrasound to enhance consolidation.

• Journal of the Korean Geotechnical Society
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• v.27 no.9
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• pp.55-66
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• 2011

The compulsory replacement method for soft ground treatment is simple but excellent in economic feasibility. However, the accurate replacement depth is not easy to properly predicted since an theoretical algorithm has not presently been established so far. In this research a prediction equation is proposed in a new form based on the liquid limit and natural moisture content rather than on the bearing capacity of the soft soil layer. The equation is based on the monitoring as well as the confirmatory boring at the site. In addition, the equation has been derived from the data obtained from the analysis of the characteristics of silt/clay of Sihwa region. The final prediction equation has been drawn by applying the regression analysis method.

• The Plant Pathology Journal
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• v.24 no.1
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• pp.63-66
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• 2008

The fermentation broth (FB) of Streptomyces griseofuscus 200401 isolated from non-farming soil showed antagonistic activity against powdery mildew fungus both in melon leaf/seedling assay and in field trials. The FB of S. griseofuscus 200401 was tested at different concentrations. In primary test, the control value of 2-fold diluted FB of S. griseofuscus 200401, compared to control, reached to 82.8%. The protective activity recorded 80.5% in 2-fold dilution of FB. The effect was reduced to 28.9% in high dilution (100-fold) treatment. The curative effect was relatively lower than protective activity. In field test, the antifungal activities of S. griseofuscus 200401 remained low in blocks sprayed with 100-fold diluted FB and the control values were 15.2 and 15.9% in 2005 and 2006, respectively. However, the activities were as high as 65% and 67.3% in the blocks treated with 2-fold dilution of FB during the same period.

• The Plant Pathology Journal
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• v.34 no.3
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• pp.218-235
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• 2018

Plant growth promoting rhizobacteria and endophytic bacteria were isolated from different varieties of turmeric (Curcuma longa L.) from South India. Totally 50 strains representing, 30 PGPR and 20 endophytic bacteria were identified based on biochemical assays and 16S rDNA sequence analysis. The isolates were screened for antagonistic activity against Pythium aphanidermatum (Edson) Fitzp., and Rhizoctonia solani Kuhn., causing rhizome rot and leaf blight diseases in turmeric, by dual culture and liquid culture assays. Results revealed that only five isolates of PGPR and four endophytic bacteria showed more than 70% suppression of test pathogens in both assays. The SEM studies of interaction zone showed significant ultrastructural changes of the hyphae like shriveling, breakage and desication of the pathogens by PGPR B. cereus (RBacDOB-S24) and endophyte P. aeruginosa (BacDOB-E19). Selected isolates showed multiple Plant growth promoting traits. The rhizome bacterization followed by soil application of B. cereus (RBacDOB-S24) showed lowest Percent Disease Incidence (PDI) of rhizome rot and leaf blight, 16.4% and 15.5% respectively. Similarly, P. aeruginosa (BacDOB-E19) recorded PDI of rhizome rot (17.5%) and leaf blight (17.7%). The treatment of these promising isolates exhibited significant increase in plant height and fresh rhizome yield/plant in comparison with untreated control under greenhouse condition. Thereby, these isolates can be exploited as a potential biocontrol agent for suppressing rhizome rot and leaf blight diseases in turmeric.

• Plant Resources
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• v.4 no.2
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• pp.65-74
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• 2001

Coleus forskohlii Briq, of the family Lamiaceae yields a valuable secondary metabolity known as forskolin which is a labdane diterpenoid.. Coleus forskohlii is the only known source of this compound. Forskolin is used in medicine for the treatment of glaucoma, congestive cardiomyopathy and asthma. Morphogenic callus was induced from young leaves on MS medium augmented with NAA and BA. These calli, when subcultured on MS with KN alone gave rise to shoots. The regenerated shoot developed good root system on MS medium fortified with NAA. The fully grown plantlets were transferred to soil for acclimatization. Coleus plant is mainly infected by a fungi Lasiodiplodia theobromae which causes root rot disease. The fungal culture filterate (ECE) of Lasiodiplodia theobromae, has been used in regeneration media to find the MIC and further to select resistant plants to the pathogen. In the present study 40% ECF in the medium showed maximum inhibition and is there fore considered as the MIC level of Coleus forshohlii. This data could prove to be useful for the future for selecting a resistant C.forskohlii plant against the root disease caused by L. theobromae.