Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Rapid Micropropagation of Hovenia dulcis Thunb. Through in vitro Stem Nodal Cultures

  • Park, Dong-Jin (Division of Environmental Forest Science, Gyeongsang National University) ;
  • Kang, Young-Min (Department of Forestry, Mississippi State University) ;
  • Jung, Ha-Na (Division of Environmental Forest Science, Gyeongsang National University) ;
  • Min, Ji-Yun (Division of Environmental Forest Science, Gyeongsang National University) ;
  • Kim, Yong-Duck (Division of Environmental Forest Science, Gyeongsang National University) ;
  • Karigar, Chandrakant S. (Division Biochemistry, Department of Chemistry, Central Collage Campus, Bangalore University) ;
  • Choi, Myung-Suk (Division of Environmental Forest Science, Gyeongsang National University)
  • Received : 2005.09.14
  • Accepted : 2005.11.17
  • Published : 2006.06.30
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Abstract

An efficient method for in vitro propagation of the medicinal plant Hovenia duleis, was established. Plantlets for micropropagation of H. dulcis were obtained from in vitro germinated seeds. The effectiveness of various levels of cytokinins (BAP, Kinetin and TDZ) on multiple shoot formation from stem nodes was tested. BAP (1.0 mg/L) treatment induced highest number of multiple shoots. The growth pattern of plantlet on various culture media was undertaken. The shoot elongation was optimal on 2MS basal medium without growth regulators. The in vitro rooting ability of H. dulcis shoots was examined with two-auxins IAA and IBA. The IAA (1.0 mg/L) treatments induced earliest rooting with maximum number of roots and root growth. Rooted shoots were transferred directly to small pots with artificial soil and such established plant exhibited a normal growth pattern similar to wild plantlet.

Keywords

References

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