• Korean Journal of Microbiology
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• v.54 no.4
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• pp.428-435
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• 2018

In this study, we isolated and identified bacteria from freshwater and soil collected from Osang reservoir, to screen antimicrobial bacteria against various pathogenic bacteria. 38 strains were isolated and assigned to the class Proteobacteria (22 strains), Actinobacteria (7 strains), Bacteroidets (6 strains), and Firmicutes (3 strains) based on 16S rRNA gene sequence analysis. Among them, strain OS17 showed a good growth inhibition against 5 methicillin-resistant Staphylococcus aureus subsp. aureus strains and Bacillus cereus, Bacillus subtilis, Filobasidium neoformans. As a result of the 16S rRNA gene sequence analysis, strain OS17 show the high similarity with Burkholderia ambifaria $AMMD^T$, B. diffusa $AM747629^T$, B. tettitorii $LK023503^T$ 99.8%, 99.7%, 99.6%, respectively. We investigated cell growth and antimicrobial activity according to commercial culture medium, temperature, pH for culture optimization of strain OS17. Optimal conditions for growth and antimicrobial activity in strain OS17 were found to be: YPD medium, $35^{\circ}C$ and pH 6.5. When the strain was cultured in LB, NB, TSB, R2A media at $20^{\circ}C$ and $25^{\circ}C$, the antimicrobial activity did not show. Culture filtrate of strain OS17 showed antimicrobial activity against 5 MRSA strains, Bacillus cereus, Bacillus subtilis, and Filobasidium neoformans with inhibition zones from 2 to 8 mm. Optimal reaction time was 48 h in YPD medium, 100 rpm and 0.3 vvm in 2 L-scale fed-batch fermentation process for antimicrobial activity. Culture optimization of strain OS17 can be improved on antimicrobial activity. Therefore, the antimicrobial activity of Burkholderia sp. OS17 had potential as antibiotics for pathogens including MRSA.

• Journal of the Korean Geotechnical Society
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• v.24 no.6
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• pp.57-67
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• 2008

Two test methods, nonrepetitive plate loading test (NPLT) and repetitive plate loading test (RPLT) are being used to control the quality of compaction through the evaluation of the stiffness of subgrade soils in the Korea railway industry. Subgrade reaction modulus ($k_{30}$) from the NPLT and strain modulus ($E_v$) from the RPLT are the index values to check them. The methods have similar aspects, but they differ in the modulus evaluation method, the numbers of loading stage, termination procedures, etc. This paper analyses the differences of the two test methods and evaluates the relationship between subgrade reaction modulus and strain modulus. In order to develop the relationship, total 22 tests were performed including the NPLT and the RPLT at the 6 original grounds, and 5 upper or lower subgrades in Kyungbu High Speed Railway II stage construction sites. According to the soil conditions, the relationship between subgrade reaction modulus and strain modulus was proposed with corrections by considering strain states, mean confining pressures, and Poisson's ratio.

• Journal of the Korean Geosynthetics Society
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• v.15 no.3
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• pp.1-12
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• 2016

In this study, the impact of sand and weathered granite soil is analyzed by changing the internal temperature from $20^{\circ}C{\sim}70^{\circ}C$C by installing a heating coil inside the triaxial cell. To check the effect on weathered granite soil due to increase of temperature and number of heating-coiling cycles are analyzed by measuring the temperature by using thermometer installed inside the triaxial cell and due to that deviator stress also occurred during the consolidated undrained test. To analyze the effect of weathered granite soil with change of temperature during undrained testing. The deviator stress and pore pressure is measured. As a result, pore pressure increases and the deviator stress decreases with rise of temperature.

• Journal of the Korean GEO-environmental Society
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• v.11 no.4
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• pp.25-31
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• 2010

The amount of dredging clay will be greatly increased by the eco-rive project and port development in Korea. Geotechnical engineers have thrown their efforts into the new ways for effective re-uses of the dredging clay such as the material for reclamation, and so on. However, very high initial water content and low strength causes unexpected difficulties in the aspect of trafficablility or time for consolidation. Therefore, the injection of cement stabilizer is used as one of ways to improve reclaimed ground. However, it also makes an argument by heavy metal from cement stabilizer. In this paper constant rate of strain consolidation test and normal consolidation test were performed to investigate behavior characteristics of the consolidation about soil-cement include lean mixed cement to reduce the environmental loads by the cement. The experimental results of consolidation characteristics about soil-cement include lean mixed cement influenced by mixing ratio. Especially it was observed that mixing ratio of 4%~6% leads not only the reduction of consolidation settlement, but time for consolidation.

• Journal of Soil and Groundwater Environment
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• v.11 no.1
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• pp.1-6
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• 2006

The analysis of functional population and its dynamics on the environment is essential for understanding bioremediation in environment. Here, we report a method for oligonucleotide microarray for the monitoring of aliphatic and aromatic degradative genes. This microarray contained 15 unique and group-specific probes which were based on 100 known genes involved pathways in biodegradation. Hybridization specificity tests with pure cultures, strain Pseudomonas aeruginosa KCTC 1636 indicated that the designed probes on the arrays appeared to be specific to their corresponding target genes. It was found that the presence of 8 genes encoding alkane, naphthalene, biphenyl, pyrene (PAH ring-hydroxylating) degradation pathway could be detected in oil contaminated soil sample. Therefore, the findings of this study strongly suggest that oligonucleotide microarray is an effective diagnostic tool for evaluating biodegradation capability in oil contaminated subsurface environment.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.17 no.1
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• pp.41-46
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• 2005

The seismic response characteristics of a port structure were investigated by the earthquake analyses of the structure subjected to high-, low-frequency component, and Uljin earthquakes. In the Fluid-Structure-Soil Interaction(FSSI) analysis, the fluid is modeled by the 4-node quadrilateral element which is a modification of a structural plane element, and the port structure and foundation is modelled by the plane strain element. Since the present method directly models the fluid-structure-soil interaction system using finite element method, it can be easily applied to the dynamic analysis of a 2-D fluid-port-soil system with complex geometry. The results of the seismic coefficient. added mass, and FSSI methods are compared. The results showed that the earthquake with high frequency components more affects the seismic response of the structure than that of low frequency components.

• Journal of the Korean Geosynthetics Society
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• v.15 no.1
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• pp.11-19
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• 2016

This paper presents the results of a scale model test to the effect of heat exchanger drainage method in retaining wall of weathered granite soil. Purpose to rise in the temperature of the heat wires inside the weathered granite soil is preventing the collapse of the retaining wall and drainage smoothly moved to the drainage layer. Especially using a spray gun to simulate the rainfall since the rainfall drainage work is important for the rainfall effect on soil, find the difference about displacement of the retaining wall, change of volume water content, drainage, earth pressure and change in the strain of the geosynthetic was effected to heat exchanger within the soil. The result from applying the heat exchanger method decreased the earth pressure and displacement of the wall and increased drainage of water.

• Journal of the Korean Geotechnical Society
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• v.19 no.1
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• pp.21-29
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• 2003

In this study, practical guidelines to check the possibility of some lateral movement of piled abutment were investigated. In these tests, both the depth of soft clay and the rate of embankment construction are chosen to examine the effect on lateral soil movements. The depth of soft clay layer varies from 5.2 m to 11.6 m, and the rate of embankment construction has two types : staged construction(1m/30days, 1m/15days) and instant construction. Various measuring instruments such as LVDTs, strain gauges, pressure cells, and pore pressure transducers are installed in designed positions in ordo. to clarify the soil - pile interaction and the short and long term behavior f3. piled bridge abutments adjacent to surcharge loads. The validity of the proposed guidelines by centrifuge test was compared with the observed performance by lateral movement index, F(Japan Highway Public Corporation) and modified I index(Korea Highway Corporation). Based on the results obtained, the critical values off and modified I, as a practical guidelines, are proposed as 0.03 and 2.0, respectively.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.5 no.3
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• pp.23-30
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• 2002

In recently, using of steel reinforcements by reinforcing materials of the reinforced earth, micro-pile and root-pile etc,. is wide-spreading in the stabilizing control of cutting and embankment slopes, but the failure mechanism of reinforced earth as well as the effect of insert angles or types of reinforcement and others are not defined clearly. In this study, therefore heavy-duty direct shear tests were exercised on the reinforced soil and the non-reinforced soil, which was executed for research on the interaction of soil-reinforcement and theirs behavior. The hardness and softness and the standard sands were used for modeling of reinforced soil, the material constants for the computer simulation were estimated from the results of CD-Test. The effects of reinforcing and of friction increasing on the softness, area ratio of reinforcements is equal, were the better than them of the hardness, as well the reinforcing effects of shear strength without regard to the area ratio is much the same at $10^{\circ}$, insert angle of reinforced bar, differ from them of the existing study. Then, the results of numerical analysis showed that the behavior of reinforcements displayed bending resistance and shear resistance at $15^{\circ}$ and $30^{\circ}$, respectively. Also, the state of strain transfer was observed and the behavior of resistance mechanism on reinforcements presented almost the same them of landslides stabilizing pile.

• Geotechnical Engineering
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• v.11 no.1
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• pp.41-50
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• 1995

In situ clay soils with Ko condition have anisotropic characteristics, varying the response according to the principal stress direction upon loading. But because of their practicality and simplicity, consolidated isotropic undrained compression tests are commonly used in practice to determine the behavior of cohesive soils. In this study to investigate the anisotropic characteristics and the effects of consolidation stress states on the response of normally consolidated clay soils during shearing, triaxial compression and extension tests after consolidating the undisturbed clay soil samples, which are obtained as a block sample to normalized consolidation states under isotropic or Ko state, were carried out. As a result of tests, the anisotropy of the undrained strength was confirmed. Comparing the soil responses between isotropic and Ko consolidation, the undrained strength by isotropic consolidation is overestimated because of its higher mean consolidation pressure. And isotropic consolidation reduces the anisotropy of soil response and influences on the stress-strain behavior and pore pressure response because the animotropic soil structure is partially collapsed during isotropic consolidation process. Also, OCR in overconsolidated soils is decreased by isotropic consolidatiorL Friction angle in eztension is higher than that in compression, but regression analysis shows that friction angle with cohesion in extension is almost the same as that without cohesion in compresslon.