• Korean Journal of Soil Science and Fertilizer
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• v.43 no.4
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• pp.490-497
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• 2010

A chitinolytic bacterium having a strong antagonistic activity against various pathogens including Phytophtora capsici was isolated from rhizosphere soil, and identified as Lysobacter enzymogenes (named as LE429) based on 16S rRNA gene sequence analysis. This strain produced a number of substances such as chitinase, ${\beta}-1$, 3-glucanase, lipase, protease, gelatinase and an antibiotic compound. This antibiotic compound was purified by diaion HP-20, sephadex LH-20 column chromatography and HPLC. The purified compound was identified as phenylacetic acid by gas chromatography-electron ionization (GC-EI) and gas chromatography-chemical ionization (GC-CI) mass spectrometry. In field experiment, pepper plants were treated by the strain LE429 culture (CB), neem oil solution (NO), combination (CB+NO) or control (CON). Plant height and number of branches, flowers and pods of pepper plant in CB treatment were generally highest, and followed by CB+NO, CON and NO. The fungal pathogens were strongly inhibited, while several insect pests were discovered in CB treatment. Any insect pests were not found, while all fungal pathogens tested were not suppressed in NO treatment. However, in CB+NO treatment, non incidence of fungal pathogens and insect pests were found. The strain LE429 producing secondary metabolites with neem oil should be a potential agent to control fungal diseases and insect pests.

• Journal of the Korean Geotechnical Society
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• v.31 no.3
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• pp.63-72
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• 2015

This paper describes the correlation and relationship between elastic moduli measured by three stiffness measurement methods with different mechanical characteristics to evaluate the compaction characteristics of subgrade soils. The Soil Stiffness Gauge (SSG) with very small strain (${\approx}0.001%$) ranges, static Plate Loading Test (PLT) with mid-level strain (${\approx}0.01{\sim}0.1%$) ranges, and Dynamic Cone Penetrometer (DCP) using penetration resistance were implemented to measure the elastic modulus. To use the elastic modulus measured by different measurement methods with a wide range of strain in practice, it is required to identify the correlation and relationship of measured values in advance. The comparison results of the measured elastic moduli ($E_{SSG}$, $E_{PLT}$, $E_{DCP}$) using the three measurement methods for domestic and overseas subgrade soils under various conditions indicate that the evaluated elastic modulus relies on the types of soils and the level of stress condition. The correlation analysis of the measured elastic moduli except the data of cement treated soils indicates that the static elastic modulus ($E_{PLT}$) is evaluated as about 60 to 80% of the dynamic elastic modulus ($E_{SSG}$). Unusual soils such as cement treated soils are required to be corrected by the stress correction during the correlation analysis with typical soils, because these types of soils are sensitive to the stress condition when measuring the static elastic modulus ($E_{PLT}$) of soils. In addition, when considering the use of DCP data for the evaluation of the elastic modulus ($E_{DCP}$), the measured data of the elastic modulus less than 200 MPa show more reliable correlation.

• Journal of the Korean Geotechnical Society
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• v.28 no.8
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• pp.5-19
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• 2012

Vertical drains along with the preloading technique have been commonly used to enhance the consolidation rate of dredged placement formation. In practice, vertical drains are usually installed in the process of self-weight consolidation of a dredged soil deposit because this process takes considerable time to be completed, which makes conventional analytical or numerical models difficult to quantify the consolidation behavior. In this paper, we propose a governing partial differential equation and develop a numerical model for 2-D axisymmetric non-linear finite strain consolidation considering self-weight consolidation to predict the behavior of a vertical drain in the dredged placement foundation which is installed during the self-weight consolidation. In order to verify the developed model in this paper, results of the numerical analysis are compared with that of the lab-scaled self-weight consolidation test. In addition, the model verification has been carried out by comparing with the simplified method. The comparisons show that the developed model can properly simulate the consolidation of the dredged placement formation with the vertical drains installed during the self-weight consolidation. Finally, the effect of construction schedule of vertical drains and of pre-loading during the self-weight consolidation is examined by simulating an imaginary dredged material placement site with a thickness of 10 m and 20 m, respectively. This simulation infers the applicability of the proposed method in this research for designing a soil improvement in a soft dredged deposit when vertical drains and pre-loading are implemented before the self-weight consolidation ceases.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.2C
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• pp.71-79
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• 2009

This study is to develope the resilient modulus prediction model, which is the function of mean effective principal stress and axial strain, for three types of railroad trackbed materials such as crushed stone, weathered granite soil, and crushed-rock soil mixture. The model consists of the maximum Young's modulus and nonlinear values for higher strain, analogous to dynamic shear modulus. The maximum value is modeled by model parameters, $A_E$ and the power of mean effective principal stress, $n_E$. The nonlinear portion is represented by modified hyperbolic model, with the model parameters of reference strain, ${\varepsilon}_r$ and curvature coefficient, a. To assess the performance of the prediction models proposed herein, the elastic response of a test trackbed near PyeongTaek, Korea, was evaluated using a 3-D elastic multilayer computer program (GEOTRACK). The results were compared with measured elastic vertical displacement during the passages of freight and passenger trains at two locations, whose sub-ballasts were crushed stone and weathered granite soil, respectively. The calculated vertical displacements of the sub-ballasts are within the order of 0.6mm, and agree well with measured values. The prediction models are thus concluded to work properly in the preliminary investigation.

• Journal of the Korean Applied Science and Technology
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• v.41 no.2
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• pp.199-207
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• 2024

We conducted to investigate both plant growth-promoting and plant disease-controlling activities of bacterial strains isolated from soil. Among the 48 isolated strains, SH-23, SH-26, SH-29, and SH-33 were identified as excellent strains for the production of β-glucosidase, cellulase, amylase, and protease. These 4 strains exhibited antifungal activity against plant pathogenic fungi (Botrytis cinerea, Rhizoctonia solani, Fusarium oxysporum, Colletotrichum acutatum). Strain SH-26, which exhibited excellent organic matter decomposition and antifungal activity against plant pathogenic fungi, was selected as the final superior strain. Upon determining the 16S rRNA gene sequence of the selected SH-26 strain, it exhibited 100% similarity with Pseudomonas knackmussii HG322950 B13^T, Pseudomonas citronellolis BCZY01000096 NBRC 103043^T, and Pseudomonas delhiensis jgi.1118306 RLD-1^T. Furthermore, it was confirmed that the Pseudomonas sp. SH-26 exhibited siderophore production, nitrogen fixation ability, and the production of Indole-3-acetic acid.

• Geotechnical Engineering
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• v.12 no.4
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• pp.157-178
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• 1996

Current design methods for reinforced earth structures take no account of the magnitude of the strains induced in the tensile members as these are invariably manufactured from high modulus materials, such as steel, where straits are unlikely to be significant. With fabrics, however, large strains may frequently be induced and it is important to determine these to enable the stability of the structure to be assessed. In the present paper internal design method of analysis relating to the use of fabric reinforcements in reinforced earth structures for both stress and strain considerations is presented. For the internal stability analysis against rupture and pullout of the fabric reinforcements, a strain compatibility analysis procedure that considers the effects of reinforcement stiffness, relative movement between the soil and reinforcements, and compaction-induced stresses as studied by Ehrlich 8l Mitchell is used. I Bowever, the soil-reinforcement interaction is modeled by relating nonlinear elastic soil behavior to nonlinear response of the reinforcement. The soil constitutive model used is a modified vertsion of the hyperbolic soil model and compaction stress model proposed by Duncan et at., and iterative step-loading approach is used to take nonlinear soil behavior into consideration. The effects of seepage pressures are also dealt with in the proposed method of analy For purposes of assessing the strain behavior oi the fabric reinforcements, nonlinear model of hyperbolic form describing the load-extension relation of fabrics is employed. A procedure for specifying the strength characteristics of paraweb polyester fibre multicord, needle punched non-woven geotHxtile and knitted polyester geogrid is also described which may provide a more convenient procedure for incorporating the fablic properties into the prediction of fabric deformations. An attempt to define improvement in bond-linkage at the interconnecting nodes of the fabric reinforced earth stracture due to the confining stress is further made. The proposed method of analysis has been applied to estimate the maximum tensions, deformations and strains of the fabric reinforcements. The results are then compared with those of finite element analysis and experimental tests, and show in general good agreements indicating the effectiveness of the proposed method of analysis. Analytical parametric studies are also carried out to investigate the effects of relative soil-fabric reinforcement stiffness, locked-in stresses, compaction load and seepage pressures on the magnitude and variation of the fabric deformations.

• Korean Journal of Environmental Biology
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• v.29 no.1
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• pp.52-60
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• 2011

Today, the weather is changing continually, due to the progress of global warming. As the weather changes, the habitats of different organisms will change as well. It cannot be predicted whether or not the weather will change with each passing day. In particular, the biological distribution of the areas climate change affects constitutes a major factor in determining the natural state of indigenous plants; additionally, plants are constantly exposed to rhizospheric microorganisms, which are bound to be sensitive to these changes. Interest has grown in the relationship between plants and rhizopheric microorganisms. As a result of this interest we elected to research and experiment further. We researched the dominant changes that occur between plants and rhizospheric organisms due to global warming. First, we used temperature as a variable. We employed four different temperatures and four different sites: room temperature ($27^{\circ}C$), $+2^{\circ}C$, $+4^{\circ}C$, and $+6^{\circ}C$. The four different sites we used were populated by the following species: Pinus deniflora, Pinus koraiensis, Quercus acutissima, and Alnus japonica. We counted colonies of these plants and divided them. Then, using 16S rRNA analysis we identified the microorganisms. In conclusion, we identified the following genera, which were as follows: 10 species of Bacillus, 2 Enterobacter species, 4 Pseudomonas species, 1 Arthrobacter species, 1 Chryseobacterium species, and 1 Rhodococcus species. Among these genera, the dominant species in Pinus deniflora was discovered in the same genus, but a different species dominated at $33^{\circ}C$. Additionally, that of Pinus koraiensis changed in both genus and species which changed into the Chryseobacrterium genus from the Bacilus genus at $33^{\circ}C$.

• The Korean Journal of Pesticide Science
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• v.10 no.4
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• pp.313-319
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• 2006

Two hundred bacterial strains were isolated from the soil around healthy tomato plants in a polyvinyl house, where most of the other plants showed bacterial wilt symptoms. The strains were screened in vitro for their antibacterial activity. Among them, a strain, KPB3 showed strong bactericidal activity against bacterial wilt pathogen, Ralstonia solanacearum. The strain KPB3 was identified using physiological and biochemical tests, and 16S rRNA analyses. Based on these tests, the strain was found to be closer to genus Paenibacillus. To control the bacterial wilt caused by R. solanacearum, greenhouse experiments were conducted to determine the effectiveness of the Paenibacillus strain KPB3. Drench application of this strain ($4{\times}10^8$ CFU $mL^{-1}$) into the pots containing tomato plants, post-inoculated with the pathogen, R. solanacearum could drastically reduce the disease severity, compared to the non-treated plants. To evaluate effectiveness of this strain under field conditions, experiments were carried out in polyvinyl houses infested with R. solanacearum, during spring and autumn of the year 2006. It was observed that, during spring, bacterial wilt was more prevalent compared to the autumn. During spring, 50.9% disease incidences occurred in non-treated controls, while, Paenibacillus strain KPB3 treated plants showed 24.6% disease incidences. Similarly, during autumn, around 17.2% plants were infected with bacterial wilt in non- treated polyvinyl houses, compared to the Paenibacillus strain KPB3 treated plants, which showed 7.0% disease incidences. These results demonstrated that, Paenibacillus strain KPB3 is a potential biological control agent against bacterial wilt caused by R. solanacearum, effective under greenhouse as well as field conditions. This is the first report showing biocontrol of R. solanacearum using a Paenibacillus spp. under field conditions.

• Proceedings of the Korean Geotechical Society Conference
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• 2003.03a
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• pp.365-368
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• 2003

Despite of the simple equipment and operation, DMT has been widely used to obtain various soil parameters and those parameters have been successfully applied to geotechnical design practice. Among them, the estimation of horizontal coefficient of consolidation is so useful that many researchs recently have been carried out. However, simulation of the penetration of the DMT blade is complex due to the inherent difficulty on analyzing a plane strain deformation of the soil around blade. Therefore, empirical and semi-empirical methods that use the theoretical solution developed fur piezocone with some assumptions have been used to estimate the coefficient of consolidation from Dilatometer dissipation test. In this paper, coefficients of consolidation c$\_$h/ which were obtained using equivalent radius that is same area with the DMT blade and optimization technique are compared with those obtained from Oedometer test and other interpretation methods. It was found that a new method used in this study can give more precise horizontal coefficient of consolidation than other methods do.

• Journal of Industrial Technology
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• v.20 no.A
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• pp.93-100
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• 2000

This research is to investigate the self-weight consolidation settlement and desiccation shrinkage settlement of soft marine dredging clay by performing numerical and experimental works. Large column test were carried out investigate the consolidation settlement considering effect of the self-weight and desiccation shrinkage, and centrifuge model test was also carried out investigate self-weight consolidation settlement. Results of centrifuge model and large column experiments about changes of settlement with time were analyzed by using the numerical technique of explicit finite difference method considering effect of the self-weight and desiccation based on the finite strain consolidation theory. Centrifuge model test results were in relatively good agreements with analyzed results in terms of self-weight consolidation settlement with time. Large column test results showed quite different values from the numerically estimated one, carried by experimental conditions.