• Journal of Microbiology
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• v.34 no.4
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• pp.370-373
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• 1996

Three strains (KW-1, KW-14, KW-15) of Bacillus thuringiensis were isolated from soil in Wonju area and characterized. The three strains produced parasporal inclusion bodies (crystals) and spores in their cells. The KW-1 strain produces spherical crystals. The crystals of strain KW-14 are bipyramidal crystal. The KW-15 strain harbors irregular crystals. Only minor biochemical characteristics of the three isolates were different and distinctive, however general characteristics were similar to the known serotypes of B. thuringiensis. Three strains were resistant to penicilin G, oxacillin and cephalothin. Three strains were highly toxic to Bombyx mori larvae, but not to the Culex pipiens larvae.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.395-398
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• 2002

Bioremediation has been showing promise as an alternative to conventional environmental cleanup technologies. The objective of this study is to maximize the degradability of jet fuel in the soil system. The cells isolated from petroleum contaminated site was used for the degradation of jet fuel. When this strain was cultured in the MSM(minimal salt media) containing jet fuel for ten days, the degradability of jet fuel was almost 100%. The concentration of jet fuel did not affect the degradability much and the increased inoculution of strain and addition of nitrogen source decreased the time for complete degradation of jet fuel in the liquid culture. Inoculation of this strain increased the jet fuel degradability in the soil column by 15% and the aeration(50ml/min) and the addition of nutrients($NaNO_3$, $KH_2PO_4$) enhanced the jet fuel degradability(about 90%).

• Microbiology and Biotechnology Letters
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• v.41 no.1
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• pp.79-87
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• 2013

An unstable yet efficient phenanthrene-degrading bacterium strain Ph-3 was isolated from a petroleum-contaminated site at the Mathura Oil Refinery, India. The strain was identified as Pseudomonas sp. using a polyphasic approach. An analysis of the intermediates and assays of the degradative enzymes from a crude extract of phenanthrene-grown cells showed a novel and previously unreported pattern of 1, 2-dihydroxy naphthalene and salicylic acid production. While strain Ph-3 lost its phenanthrene- degrading potential during successive transfers on a rich medium, it maintained this trait in oligotrophic soil conditions under the stress of the pollutant and degraded phenanthrene efficiently in soil microcosms. Although the maintenance and in vitro study of unstable phenotypes are difficult and such strains are often missed during isolation, purification, and screening, these bacteria constitute a substantial fraction of the microbial community at contaminated sites and play an important role in pollutant degradation during biostimulation or monitored natural attenuation.

• Geomechanics and Engineering
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• v.20 no.2
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• pp.121-129
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• 2020

In the design of geotechnical structures, engineers choose either peak or critical state friction angles. Unfortunately, this selection is based on engineer's preference for economy or safety and lacks the assessment of the expected level of deformation. To fill this gap in the design process, this study proposes a strain based empirical method. Proposed method is founded on the experimentally supported assumption that higher dilatancy angles result in more brittle soil response. Using numerous triaxial test data on ten different soils, an empirical design chart is developed that allows the estimation of shear strain at failure based on soil's peak dilatancy angle and mean grain diameter. Developed empirical chart is verified by conducting a small scale retaining wall physical model test. Finally, a design methodology is proposed that makes the selection of design friction angle in structured way possible based on the serviceability limits of the proposed structure.

• YAKHAK HOEJI
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• v.33 no.3
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• pp.175-182
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• 1989

To find ${\alpha}-amylase$ inhibitors produced by microorganisms from soil, a strain which had a strong inhibitory activity against bacterial ${\alpha}-amylase$ was isolated from the soil sample collected in Korea. The morphological and physiological characteristics of this strain on several media and its utilization of carbon sources showed that it was one of Streptomyces species according to the International Streptomyces Project method. The amylase inhibitor of this strain was purified by active carbon adsorption, silicagel column chromatography, SP-Sephadex C-25 column chromatography, adsorption on Amberlite XAD-2. The inhibitor was oligosaccharide which was composed of glucose. The inhibitor had inhibitory activity against other amylase such as salivary ${\alpha}-amylase$, pancreatic ${\alpha}-amylase$, fungal ${\alpha}-amylase$ and gluco-amylase.

• Archives of Pharmacal Research
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• v.10 no.2
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• pp.103-109
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• 1987

To find microorganisms producing esterase inhibitors, microbes were isolated from soil samples that were collected at different locations in Korea and screened for inhibitory activities. One of the inhibitor-producing strains was named strain DMC-498. This strain was found to be a new species of the genus Streptomyces by comparison with the characteristics of morphology and metabolisms of the other species of the genus.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 1999.10a
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• pp.91-98
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• 1999

In this study a modified ground strain model is developed for an equivalent earthquake load and is applied to the seismic analysis of buried pipelines, The ground strain can be obtained using the ratio of a maximum ground velocity to a wave propagation velocity. To reflect soil conditions and seismic characteristics the wave propagation velocity is evaluated by a proposed dispersion curve based on wave energy distribution. In order to verify the procedures the observed earthquake data and the results of this study are compared. For the application of an equivalent earthquake load to the seismic analysis the buried pipelines are modeled using the beam theory. the results of the analyses are compared with those of a dynamic analysis code and those obtained from the response displacement method. Finally various parametric studies considering different soil conditions and seismic loads are examined.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2003.09a
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• pp.107-114
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• 2003

There are many problems in the prediction of dynamic behaviors of saturated soils because undrained excess pore water pressure builds up and then the strain softening behavior is occurred simultaneously. A few analytical constitutive models based on the effective stress concept have been proposed but most models hardly predict the excess pore water pressure and strain softening behaviors correctly In this study, the disturbed state concept (DSC) model proposed by Dr, Desai was modified to predict the saturated soil behaviors under the dynamic loads. Also, back-prediction program was developed for verification of modified DSC model. Cyclic triaxial tests were carried out to determine DSC parameters and test result was compared with the result of back-prediction. Through this research, it is proved that the proposed model based on the modified disturbed state concept can predict the realistic soil dynamic characteristics such as stress degradation and strain softening behavior according to dynamic process of excess pore water pressure.

• The Korean Journal of Mycology
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• v.49 no.2
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• pp.175-181
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• 2021

A fungal strain was isolated from a soil sample collected in Korea and designated as YW23-8. Based on a sequence analysis of the internal transcribed spacer (ITS) regions, the isolate was assigned to the genus Sarcopodium. Moreover, a phylogenetic analysis based on the concatenated nucleotide sequences of the ITS regions and the large subunit of the nuclear ribosomal RNA (LSU) gene showed that the strain YW23-8 occupies a distinct phylogenetic position within Sarcopodium. The isolate had significant differences from its closest neighbors, S. circinosetiferum, S. circinatum, S. macalpinei, and S. vanillae. Morphological features such as different conidial structures, the absence of septation in conidia, and the presence of milky white watery droplets along with the results of the phylogenetic analysis clearly distinguish YW23-8 from the closest Sarcopodium species. We therefore conclude that strain YW23-8 represents a novel species of the genus Sarcopodium for which we propose the name Sarcopodium terrigenum.

• Journal of the Korean Geotechnical Society
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• v.18 no.6
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• pp.83-101
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• 2002

Elastic waves provide an important information about the soil mass in the near-surface. Soil properties in relation to elastic wave parameters are clarified to facilitate the application of geophysical technique to soil characterization. As an example, experiments are performed to gain further insight into the behavior of unsaturated particulate materials using bender elements. The small strain stiffness is continuously measured on specimens subjected to drying, and changes in stiffness are related to changes in interparticle forces such as capillarity, bonding due to ion sharing, buttress effect due to fine migration, and cementation due to salt precipitation. The rate of menisci regeneration is studied after a perturbation as well. Finally, several phenomena associated with the evolution of capillary forces during drying are identified.