The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Phylogeny and Morphology of Sarcopodium terrigenum sp. nov., a Novel Fungal Species Isolated from Soil in Korea

  • Elderiny, Nabil Salah (School of Applied Biosciences, Kyungpook National University) ;
  • Das, Kallol (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, Seung-Yeol (School of Applied Biosciences, Kyungpook National University) ;
  • Jung, Hee-Young (School of Applied Biosciences, Kyungpook National University)
  • Received : 2021.03.29
  • Accepted : 2021.05.28
  • Published : 2021.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

A fungal strain was isolated from a soil sample collected in Korea and designated as YW23-8. Based on a sequence analysis of the internal transcribed spacer (ITS) regions, the isolate was assigned to the genus Sarcopodium. Moreover, a phylogenetic analysis based on the concatenated nucleotide sequences of the ITS regions and the large subunit of the nuclear ribosomal RNA (LSU) gene showed that the strain YW23-8 occupies a distinct phylogenetic position within Sarcopodium. The isolate had significant differences from its closest neighbors, S. circinosetiferum, S. circinatum, S. macalpinei, and S. vanillae. Morphological features such as different conidial structures, the absence of septation in conidia, and the presence of milky white watery droplets along with the results of the phylogenetic analysis clearly distinguish YW23-8 from the closest Sarcopodium species. We therefore conclude that strain YW23-8 represents a novel species of the genus Sarcopodium for which we propose the name Sarcopodium terrigenum.

Keywords

Acknowledgement

This research was supported by Kyungpook National University Development Project Research Fund, 2018

References

  1. Lombard L, Van der Merwe NA, Groenewald JZ, Crous PW. Generic concepts in Nectriaceae. Stud Mycol 2015;80:189-245. https://doi.org/10.1016/j.simyco.2014.12.002
  2. Rossman AY, Allen WC, Braun U, Castlebury LA, Chaverri P, Crous PW, Hawksworth DL, Hyde KD, Johnston P, Lombard L, et al. Overlooked competing asexual and sexually typified generic names of Ascomycota with recommendations for their use or protection. IMA Fungus 2016;7:289-308. https://doi.org/10.5598/imafungus.2016.07.02.09
  3. Sutton BC. Sarcopodium and its synonyms. Trans Br Mycol Soc 1981;76:97-102. https://doi.org/10.1016/S0007-1536(81)80012-5
  4. Liu DY, Paterson RRM. Sarcopodium. Molecular detection of human fungal pathogens. 1st ed. London: Taylor and Francis Group; 2011. p. 485-8.
  5. Guarro J, Hofling-Lima AL, Gene J, De Freitas D, Godoy P, Zorat-Yu ML, Zaror L, Fischman O. Corneal ulcer caused by the new fungal species Sarcopodium oculorum. J Clin Microbiol 2002;40:3071-5. https://doi.org/10.1128/JCM.40.8.3071-3075.2002
  6. Raj T, George K. A new species of Sarcopodium on coffee from India. Curr Sci 1960;29:192-3.
  7. Matsuo H, Nonaka K, Nagano Y, Yabuki A, Fujikura K, Takahashi Y, Omura S, Nakashima T. New metabolites, sarcopodinols A and B, isolated from deep-sea derived fungal strain Sarcopodium sp. FKJ-0025. Biosci Biotechnol Biochem 2018;27:1-4.
  8. Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 1993;2:113-8. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  9. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: A guide to methods and applications. In Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. San Diego: Academic Press; 1990. p. 315-22.
  10. De Hoog GS, van den Ende AHGG. Molecular diagnostics of clinical strains of filamentous basidiomycetes. Mycoses 1998;41:183-9. https://doi.org/10.1111/j.1439-0507.1998.tb00321.x
  11. Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 1990;172:4238-46. https://doi.org/10.1128/jb.172.8.4238-4246.1990
  12. Hall TA. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999;41:95-8.
  13. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The Clustal X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;24:4876-82.
  14. Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870-4. https://doi.org/10.1093/molbev/msw054
  15. Felsenstein J. Confidence limit on phylogenies: An approach using the bootstrap. Evolution 1985;39:783-91. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x