Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Role of Unstable Phenanthrene-Degrading Pseudomonas species in Natural Attenuation of Phenanthrene-Contaminated Site

  • Prakash, Om (Microbial Culture Collection, National Centre for Cell Science) ;
  • Lal, Rup (Department of Zoology, University of Delhi)
  • Received : 2012.07.17
  • Accepted : 2012.10.25
  • Published : 2013.03.28
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Abstract

An unstable yet efficient phenanthrene-degrading bacterium strain Ph-3 was isolated from a petroleum-contaminated site at the Mathura Oil Refinery, India. The strain was identified as Pseudomonas sp. using a polyphasic approach. An analysis of the intermediates and assays of the degradative enzymes from a crude extract of phenanthrene-grown cells showed a novel and previously unreported pattern of 1, 2-dihydroxy naphthalene and salicylic acid production. While strain Ph-3 lost its phenanthrene- degrading potential during successive transfers on a rich medium, it maintained this trait in oligotrophic soil conditions under the stress of the pollutant and degraded phenanthrene efficiently in soil microcosms. Although the maintenance and in vitro study of unstable phenotypes are difficult and such strains are often missed during isolation, purification, and screening, these bacteria constitute a substantial fraction of the microbial community at contaminated sites and play an important role in pollutant degradation during biostimulation or monitored natural attenuation.

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References

  1. Balashova, N. V., A. Stolz, H. J. Knackmuss, I. A. Kosheleva, A. V. Naumov, and A. M. Boronin. 2001. Purification and characterization of a salicylate hydroxylase involved in 1-hydroxy- 2-naphthoic acid hydroxylation from the naphthalene and phenanthrene- degrading bacterial strain Pseudomonas putida BS202- P1. Biodegradation 12: 179-188. https://doi.org/10.1023/A:1013126723719
  2. Fiest, C. F. and D. Hegeman. 1969. Phenol and benzoate metabolism by Pseudomonas putida: regulation of tangential pathways. J. Bacteriol. 100: 868-877.
  3. Gentry, T., C. Rensing, and I. Pepper. 2004. New Approaches for Bioaugmentation as a Remediation Technology. Crit. Rev. Environ. Sci. Technol. 48: 447-494.
  4. Hegman, G. D. 1966. Synthesis of the enzyme of the mandelate pathway by Pseudomonas putida. J. Bacteriol. 91: 1140- 1154.
  5. Iwabuchi, T. and S. Harayama. 1998. Biochemical and molecular characterization of 1- hydroxy-2-naphthoate dioxygenase from Nocordioides sp. KP7. J. Biol. Chem. 273: 8332-8336. https://doi.org/10.1074/jbc.273.14.8332
  6. Iwabuchi, T. and S. Haryana. 1997. Biochemical and genetic characterization of 2- carboxylbenzaldehyde-dehydrogenase an enzyme involved in phenanthrene degradation by Nocardioides sp. strain KP7. J. Bacteriol. 179: 6488-6494.
  7. Jermy, A. 2010. Bioremediation: Seek and destroy. Nat. Rev. Microbiol. 8: 465. https://doi.org/10.1038/nrmicro2401
  8. Kiyohara, H., K. Nagao, K. Kouno, and Y. Yano. 1982. Phenanthrene degrading phenotype of Alcaligenes faecalis AFK2. Appl. Environ. Microbiol. 43: 458-461.
  9. Kostka, J. E., O. Prakash, W. A. Overholt, S. J. Green, G. Freyer, A. Canion, J. Delgardio, N. Norton, T. C. Hazen, and M. Huettel. 2011. Hydrocarbon-degrading bacteria and the bacterial community response in Gulf of Mexico beach sands impacted by the deepwater horizon oil spill. Appl. Environ. Microbiol. 77: 7962-7974. https://doi.org/10.1128/AEM.05402-11
  10. Li, J., T. Wang, B. Shao, J. Shen, S. Wang, and Y. Wu. 2012. Plasmid-mediated quinolone resistance genes and antibiotic residues in wastewater and soil adjacent to swine feedlots: Potential transfer to agricultural lands. Environ. Health Perspect 120: 1144-1149. https://doi.org/10.1289/ehp.1104776
  11. Liu, Y., J. Zhang, and Z. Zhang. 2004. Isolation and characterization of polycyclic aromatic hydrocarbons-degrading Sphingomonas sp. strain ZL5. Biodegradation 15: 205-212. https://doi.org/10.1023/B:BIOD.0000026579.38741.e1
  12. Mona, L., T. Omori, and T. Kodama. 1993. Microbial degradation of dibenzofuran, fluorine and dibenzo-p-dioxin by Staphylococcus auriculans DBF63. Appl. Environ. Microbiol. 59: 285-289.
  13. Patel, V., S. Cheturvedula, and D. Madamwar. 2012. Phenanthrene degradation by Pseudoxanthomonas sp. DMVP2 isolated from hydrocarbon contaminated sediment of Amlakhadi canal, Gujarat, India. J. Hazard. Mater 201: 43-51.
  14. Patnaik, P. 1992. Hydrocarbon, aromatic. In: A Comprehensive guide to the hazardous properties of chemical substances, (Van Nostrand Reinhold ed.), New York. pp. 429-445.
  15. Pertsova, R. N., B. P. Baskunov, and L. A. Golovleva. 1982. Oxidation characteristic of aromatic acids formed in DDT breakdown by a Pseudomonas aeruginosa culture. Mikrobiologiia 51: 275-280.
  16. Prabhu, Y. and P. S. Phale. 2003. Biodegradation of phenanthrene by Pseudomonas sp. strain PP2: noval metabolic pathway, role of biosurfactant and cell surface hydrophobicity in hydrocarbon assimilation. Appl. Microbiol. Biotechnol. 6: 1 342-351.
  17. Prakash, O. and R. Lal. 2006. Phenanthrene-degrading bacterium from fly ash dumping site, Sphingobium fuliginis sp. nov. and reclassification of Sphingomonas cloacae as Sphingobium cloacae comb. nov. Int. J. Syst. Evol. Microbiol. 56: 2147-2152. https://doi.org/10.1099/ijs.0.64080-0
  18. Prakash, O., K. Kumari, and R. Lal. 2007. Pseudomonas delhiensis sp. nov., from a fly ash dumping site of a thermal power plant. Int. J. Syst. Evol. Microbiol. 57: 527-531. https://doi.org/10.1099/ijs.0.64456-0
  19. Prakash, O., T. M. Gihring, D. D. Dalton, K. J. Chin, S. J. Green, D. M. Akob, G. Wanger, and J. E. Kostka. 2010. Geobacter daltonii sp. nov., an Fe (III)- and uranium(VI)-reducing bacterium isolated from a shallow subsurface exposed to mixed heavy metal and hydrocarbon contamination. Int. J. Syst. Evol. Microbiol. 60: 546-553. https://doi.org/10.1099/ijs.0.010843-0
  20. Rodrigues, A. C., S. Wuertz, A. G. Brito, and L. F. Melo. 2005. Fluorene and phenanthrene uptake by Pseudomonas putida ATCC 17514: kinetics and physiological aspects. Biotechnol. Bioeng. 90: 281-289. https://doi.org/10.1002/bit.20377
  21. Samanta, S. K., A. K. Chakraborti, and R. K. Jain. 1999. Degradation of Phenanthrene by different bacteria: evidence for novel transformation sequences involving the formation of 1- naphthol. Appl. Microbiol. Biotechnol. 53: 98-107. https://doi.org/10.1007/s002530051621
  22. Sasser, M. 1990. Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. Newark, DE: MIDI Inc.
  23. Smith, M. A. and M. J. Bidochka. 1998. Bacterial fitness and plasmid loss: The importance of culture conditions and plasmid size. Can. J. Microbiol. 44: 351-355. https://doi.org/10.1139/w98-020
  24. Tyagi, M., Manuela M., da Fonseca, R. and de Carvalho, C C. C. R. 2011. Bioaugmentation and biostimulation strategies to improve the effectiveness of bioremediation processes. Biodegradation 22: 231-241. https://doi.org/10.1007/s10532-010-9394-4
  25. Van Veen, J. A., L. S. van Overbeek, and J. D. van Elsas. 1997. Fate and activity of microorganism introduced into soil. Microbiol. Mol. Biol. Rev. 62: 121-135.
  26. Wang, S., N. Nomura, T. H. Nakajima, and H. Uchiyama. 2012. Case study of the relationship between fungi and bacteria associated with high-molecular-weight polycyclic aromatic hydrocarbon degradation. J. Biosci. Bioeng. 113: 624-630. https://doi.org/10.1016/j.jbiosc.2012.01.005
  27. Watve, M. M., N. Dahanukar, and M. G. Watve. 2010. Sociobiological control of plasmid copy number in bacteria. PLoS ONE 5: e9328. https://doi.org/10.1371/journal.pone.0009328
  28. William, F. G. and E. J. Galen. 1988. Two-stage mineralization of phenanthrene by estuarine enrichment culture. Appl. Environ. Microbiol. 54: 929-936.
  29. Wong, J. W. C., K. M. Lai, C. K. Wan, K. K. Ma, and M. Fang. 2002. Isolation and optimization of PAH degradative bacteria from contaminated soil for PAH bioremediation. Water Air Soil Pollut. 139: 1-13. https://doi.org/10.1023/A:1015883924901
  30. Yamamoto, S., M. Katagiri, H. Meno, and O. Hayaishi. 1965. Salicylate hydroxylase, a monooxygenase requiring flavin adenine dinucleotide. J. Biol. Chem. 240: 3408-3413.
  31. Zhang, T., X.-X. Zhang, and L. Ye. 2011. Plasmid metagenome reveals high levels of antibiotic resistance genes and mobile genetic elements in activated sludge. PLoS ONE 6: e26041. https://doi.org/10.1371/journal.pone.0026041
  32. Zhao, H. P., S. H. Liang, and X. Yang. 2011. Isolation and characterization of catechol 2, 3-dioxygenase genes from phenanthrene degraders Sphingomonas, sp. ZP1 and Pseudomonas sp. ZP2. Environ. Technol. 33: 1895-901.
  33. Zhou, L., X. Sheng, S. Zhang, and J. Liu. 2005. Screening of phenanthrene-degrading bacterium and its degradation conditions. Ying Young Sheng Tai Xue Bao (in Chinease) 16: 2399- 2402.