• Proceedings of the Korean Society of Applied Pharmacology
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• 1998.11a
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• pp.135-135
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• 1998

To find an antagonist of Pythium ultimum, the causal agent of damping-off, numerous actinomycete strains were screened for in vitro inhibiting mycelial growth of the target fungus and producing bioactive metabolites. A strain identified as Streptomyces sp. G60655 was isolated and used for further antagonistic efficacy. The degree of antagonism between the fungus and G60655 was affected by the medium used. Furthermore, the preinoculation of the antagonist was found to be necessary to exhibit the maximum efficacy of antagonsim against the fungus. From the culture broth, a bioactive metabolite was detected and purified by solvent extraction, silica gel chromatography and preparative HPLC. The FAB-MS spectrum of the active compound showed a molecular ion peak at m/z 1101 (M + H)$\^$+/, suggesting the molecular weight of 1100. The UV absorptions at 242 and 323 nm indicated the presence of aromatic functions. The structure of this compound was identified as echinomycin, a depsipeptide antibiotic by spectroscopic studies including various NMR measurements. Echinomycin was inactive against several soil born fungi, but inhibited the mycelial growth of P. ultimum and its related oomycetous fungi.

• Korean Journal of Microbiology
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• v.40 no.3
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• pp.211-216
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• 2004

A bacterial strain JE-ll found to produce active extracellular phospholipase D (PLD) was selected from the soil isolates. It was identified as Streptomyces somaliensis on the basis of 16S rDNA sequence analysis, morphological and physiological characteristics. The gene (sspld) encoding S. somaliensis PLD was isolated and characterized. The open reading frame was suggested to encode 538 amino acids with a signal peptide of 33 amino acids. The deduced amino acid sequence of the sspld shared a sequence similarity of 70-88％ with PLDs of other Streptomyces sp. so far reported. The PLD converted phosphatidylcholine to phosphatidylglycerol or phosphatidylserine with the yield of 96 to 99％ (㏖/㏖), but did not act on inositol or ethanolamine as a transphosphatidylation donor.

• Korean Journal of Microbiology
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• v.28 no.4
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• pp.345-350
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• 1990

In order to ferment D-xylose directly to ethanol, Yeasts capable of utilizing D-xylose as a sole carbon source and energy source were isolated from soil, sawdust and rotten woods. Among them, the yeast strain, which showed the best ability to produce ethanol, was identified as Candida sp. L-16 isolated from rotten woods. The optimal conditions for production of ethanol were 60rpm of agitation speed, 28j.deg.C of temperature, 4.5 of initial pH and 5% of D-xylose concentration. Ethanol production was reached to maximum state for 4 days culture. Under these optimal conditions, the maximum ethanol concentration and theoretical ethanol yield were 2.4%(v/v) and 74.4% of theoretical value, respectively.

• Proceedings of the Korean Geotechical Society Conference
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• 2009.03a
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• pp.195-198
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• 2009

Rammed Aggregate Pier method is intermediate foundation of deep and shallow foundation, it has been built on world wide. But the investigation and research in domestic is not accomplished. In this paper, examined details of different spacing of piles, bearing capacities, respectively, conclude with recommendations on how RAP can be used in future needs. This documentation further provides comparisons of the laboratory test results which were obtained from differenciate the spacing of piles, namely installed rammed aggregate pier. Strain control test was conducted to determine the bearing capacities of the piers; 20mm, 30mm and 40mm diameter drilling equipment to drill holes were installed in sand at initial relative densities of 40%. By comparing different spacing of piles, in this experiment, piles are spaced structually span, form a ring shape, narrowing the distance of each other, to the center. the result shows that as diameter of pier is bigger in diameter, bearing capacity also dramatically increased due to raised stiffness. Also, the space between each piers narrowed, settlement rate of soil was decreased significantly. From the test results, as the space between each piles were getting closer, allows greater chances to have resistance to deformation, shows improved stability of structures.

• Journal of the Korean Geosynthetics Society
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• v.9 no.3
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• pp.67-75
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• 2010

The tensile strength, permeability and discharge capacity of biodegradable plastic drain boards made with poly lactic acid (PLA) have been tested and verified prior to their usage at field. Based on test results, the tensile strength of biodegradable plastic drain board made with PLA has relatively lower tensile strain and tensile strength than those of plastic drain board. Performance of PLA filter having good permeability and low opening size is proper for the filter of vertical drain board. In case of improving stiffness of PLA filter, biodegradable plastic drain board also satisfies required discharge capacity as use of vertical drain board too.

• Journal of the Korean Geotechnical Society
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• v.20 no.2
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• pp.125-130
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• 2004

In this paper, the method of parameter estimation of a mathematical constitutive model blown as the smooth elasto-plastic cap model is studied. To predict the response of the real soil using this model, the eight parameters describing the constitutive equations have to be determined. First, experimental data are obtained from simple laboratory experiments such as one dimensional confined compression test in a consolidometer and drained triaxial compression test with the Ottawa sand f3r the reference value. Then, the numerical experiments are performed in the cap model with initial guessed parameters. The optimization method is utilized to fit the model response to experimental data by minimizing the error between the laboratory and numerical responses. Special attention is given to the parameter estimation procedure of numerical triaxial test due to the difficulty of the lateral strain measurements.

• Journal of the Korean Geotechnical Society
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• v.16 no.6
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• pp.35-41
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• 2000

Residual stress is defined as a minimum stress with a large displacement of specimens and the residual stress after peak shear stress appears with displacement volume but there is no provision to select the residual stress. In the previous study, residual stress was recorded when the change of shear load is small in the condition of the strain more than 15%. But, in this study, hyperbolic function((No Abstract.see full/text), b=experimental constant) of soil test is adapted to joint of rock and the propriety is investigated. In a landslide and landsliding of artificial slope, wedge failure of tunnel with a large displacement, tests are simulated from peak stress to residual stress for safety analysis. But now. direct shear stress and triaxial compressive tests are usually performed to find out characteristics of shear stress about joint. Although these tests get a small displacement, that data of peak stress and residual stress are used for safety analysis. In this study, we tried to determine failure criteria for joints of rock using ring shear test machine. The residual stress following shear behavior was determined by the result of ring shear test and direct shear test. In conclusion, after comparing the results of the two test, we found that cohesion(c) and internal friction angle(ø) of ring shear test are 30% and 22% respectively of those of the direct shear test.

• Geomechanics and Engineering
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• v.10 no.2
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• pp.225-245
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• 2016

Settlement of foundations in permafrost regions primarily results from three physical and mechanical processes such as thaw consolidation of permafrost layer, creep of warm frozen soils and the additional deformation of seasonal active layer induced by freeze-thaw cycling. This paper firstly establishes theoretical models for the three sources of settlement including a statistical damage model for soils which experience cyclic freeze-thaw, a large strain thaw consolidation theory incorporating a modified Richards' equation and a Drucker-Prager yield criterion, as well as a simple rheological element based creep model for frozen soils. A novel numerical method was proposed for live computation of thaw consolidation, creep and freeze-thaw cycling in corresponding domains which vary with heat budget in frozen ground. It was then numerically implemented in the FISH language on the FLAC platform and verified by freeze-thaw tests on sandy clay. Results indicate that the calculated results agree well with the measured data. Finally a model test carried out on a half embankment in laboratory was modeled.

• Mycobiology
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• v.39 no.3
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• pp.182-186
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• 2011

Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.

• Journal of the Society of Disaster Information
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• v.8 no.3
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• pp.204-212
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• 2012

Lightweight soils are very economical and environment friendly materials that are valuable in field without wasting construction materials, dredged soils and clay/ silty soils during construction. Recently, the research of lightweight soils mixed with recycled material (recycled tire powder, rice husks) have been investigated. In this study the mix design factors (i.e., weight of soil, water content, foaming agent and added water) were analyzed and optimized mix design was suggested using cement content for revealing strength. For the analysis the stress-strain behavior, strength with respect to time, and experimental strength for the component of recycled material were analyzed. Finally, target strength was determined to calculate reasonable and economical mix ratio and the optimized cement content was suggested.