• Korean Journal of Agricultural Science
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• v.11 no.1
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• pp.53-60
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• 1984

The followings are the results obtained from the investigation of isolation and cultural conditions of a fungus producing antibiotic from humus, spring soil, paddy, field and etc. 1. Among fungi which were capable of antimicrobial sub stance producing at the $45^{\circ}C$, Strain H-3 was selected as test st rain because of its efficiency in antimicrobial substance producing. 2. Strain H-3 was identified as a Aspergullus fumigatus by the mophological properties. 3. The compositions of optimun media for the production of antimicrobial substance were followed;glucose 20g, $NaNO_3$ 3g, $K_2HPO_4$ 1g, $CaCl_2$ 0.5g, $MgSo_4$ 0.5g, $FeSO_4$ 0.005g. 4. Optimum culture conditions were founded to be pH 5.0, $40^{\circ}C$ respectively. 5. The maximum antimicrobial substance producing obtained in this study was showed 14mm of clear zone after 72 hrs incubation under the optimum conditions. 6. AF-c substance was active against Gram-positive, Gram-negative bacteria, and yeast but not mold in its biological properties.

• Research in Plant Disease
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• v.12 no.3
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• pp.267-271
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• 2006

Botrytis cinerea Pers. was found to be highly virulent to the grapevine plant, especially in greenhouse condition. Pseudomonas species play key roles for the biocontrol of many plant diseases especially in soil. Of the 83 isolates of Pseudomonas spp., a bacterial strain P84, isolated from tomato rhizosphere, was shown to suppress a wide range of phytopathogenic fungi in vitro. The isolate was identified as Pseudomonas putida on the basis of its bacteriological and genetic characteristics. The P. putida P84 strain carry the phlD gene for 2,4-diacetylphloroglucinol biosynthesis and may produce the antibiotics as an antagonistic mechanism involved in biocontrol. The antagonistic activity of the bacterium has a promising implication for its use as a biocontrol agent to control grapevine gray mold.

• Journal of the Korean Geotechnical Society
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• v.21 no.9
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• pp.65-75
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• 2005

Maximum shear modulus of soil is a principal parameter for the design of earth structures under static and dynamic loads. In this study, the statistical data of maximum shear moduli of silty sands in Songdo area in the west coast of Korea evaluated by various field and laboratory tests - standard penetration test (SPT), cone penetration test (CPT), self-boring pressuremeter test (SBPT), downhole test (DH), seismic cone penetration test (SCPT) and resonant column test (RC) were analyzed. Based on the measurement of shear moduli using DH which is known as maximum value at very small strain, the new empirical correlations between shear moduli and SPT or CPT values were proposed. Predictions of maximum shear moduli using the proposed correlations were compared with the data obtained from DH. The good agreement confirmed that the proposed correlations reasonably predicted the maximum shear moduli of silty sands in the area.

• Journal of the Korean Geotechnical Society
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• v.21 no.9
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• pp.53-64
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• 2005

For geotechnical design in practice, soils are, in general, assumed to behave as a linear elastic or perfect plastic material. More realistic geotechnical design, however, should take into account various factors that affect soil behavior in the field, such as non-linearity of stress-strain response, stress history, and water content. In this study, a series of laboratory tests including triaxial and resonant column tests were peformed with sands of various silt contents, relative densities, stress states, OCR and water contents. This aims at investigating effects of various factors that affect strength and stiffness of sands. From the results in this study, it is found that the effect of OCR is significant for the intermediate stress-strain range from the initial to failure, while it may be ignored for the initial stiffness and peak strength. For the effect of water content, it is observed that the initial elastic modulus decreases with increasing water content at lower confining stress and relative density At higher confining stresses, the effect of water content Is found to become small.

• Journal of the Korean Geotechnical Society
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• v.19 no.4
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• pp.223-233
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• 2003

Sand compaction pile (SCP) is one of the ground improvement techniques which are being used for not only accelerating consolidation but also increasing bearing capacity of loose sands or soft clay grounds. In this study, laboratory model tests and 3-D finite element analyses were performed to investigate the interaction between sand compaction piles and surrounding soft soils. Based on the results obtained, as the area replacement ratio increases, the stress concentration ratio increases at the pile point, the settlement decreases, and the relative displacement between column and soil also decreases. It is also found that numerical study is illustrated by good comparison with model test results, and the numerical analysis revealed slip effects which could not be specifically identified in the model tests.

• Journal of the Korean Geotechnical Society
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• v.20 no.8
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• pp.5-14
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• 2004

The settlement of foundations under working load conditions is an important design consideration. Well-designed foundations induce stress-strain states in the soil that are neither in the linear elastic range nor in the range usually associated with perfect plasticity. Thus, in order to accurately predict working settlements, analyses that are more realistic than simple elastic analyses are required. The settlements of footings in sand are often estimated based on the results of in-situ tests, particularly the standard penetration test (SPT) and the cone penetration test (CPT). In this paper, we analyze the load-settlement response of vertically loaded footings placed in sands using both the finite element method with a non-linear stress-strain model and the conventional elastic approach. Based on these analyses, we propose a procedure for the estimation of footing settlement in sands based on CPT results.

• Journal of Korean Tunnelling and Underground Space Association
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• v.9 no.2
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• pp.143-155
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• 2007

The earth pressure acting on a circular shaft wall is smaller than that acting on the wall in plane strain condition due to the three dimensional axi-symmetric arching effect. Accurate estimation of the earth pressure is required for the design of the shaft wall. In this study, the stress model considering the decrease of earth pressure due to the horizontal and vertical arching effect and the influence of shape ratio (shaft height/radius) is proposed. In addition, model test on the sandy soil is conducted and a comparison is made between the stress model and the test results. The comparison shows that the proposed stress model is in agreement with test results; decrease of shape ratio (increase of radius) leads to stress state equal to the plane strain condition and approximate stress distribution is found between stress model and model test results.

• Journal of Microbiology and Biotechnology
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• v.9 no.5
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• pp.687-690
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• 1999

Bacillus stearothermophilus No. 236 isolated from the soil is a strong xylan degrader producing all the xylanolytic enzymes. However, the strain was discovered to be highly intractable to its transformation. In the present study, we have developed a reliable method for transformation of B. stearothermophilus No. 236 by a systematic examination of several factors which might have an influence on the efficiency of electrotransformation. Notably, we found that the most critical factor influencing the transformation efficiency (TE) was the incubation temperature after pulsing, with its optimum incubation of $37^{\circ}C.\; At\; 50^{\circ}C$, the optimum growth temperature of the B. stearothermophilus strain, the transformants could not be obtained at a recognizable level. The combination of field strength of 7.5 kV/cm along with pulse duration of 10 msec (resistance of $400{\Omega}\; and\; capacitance\; of\; 25{\mu}F$) was shown to be the best electrical parameters at the incubation temperature of $37^{\circ}$. A higher TE was obtained when the cells were harvested at an early-exponential phase. Twenty percent of PEG-8000 in a suspension buffer and an addition of 0.1% glycine in the growth medium resulted in about 4-fold and 3-fold increases in TE, respectively. We also found that the plasmid DNA which had been cycled through the host B. stearothermophilus cells enhanced TE by one order of magnitude higher. Under the presently described conditions, $2.5{\times}10^{5} transformants per ${\mu}g$ DNA was attained.

• Journal of Species Research
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• v.4 no.2
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• pp.109-126
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• 2015

As a subset study to discover indigenous prokaryotic species in Korea, a total of 39 bacterial strains assigned to the classes Betaproteobacteria and Gammaproteobacteria were isolated from diverse environmental samples collected from soil, tidal flat, freshwater, seawater, seaweed, wetland, plant roots, guts of insects, and fermented foods. From the high 16S rRNA gene sequence similarity (>99.1%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to each independent and predefined bacterial species. There is no official report that these 39 species have been described in Korea; therefore 4 species of 4 genera in the order Burkholderiales and 1 species in the order Neisseriales within the class Betaproteobacteria, and 10 species of 6 genera in the order Alteromonadales, 11 species of 3 genera in the order Pseudomonadales, 4 species of 4 genera in the order Enterobacteriales, 2 species of 2 genera in the order Vibrionales, 1 species in the order Aeromonadales, 3 species of 3 genera in the order Oceanospirillales, 2 species of 2 genera in the order Xanthomonadales, and 1 species in the order Chromatiales within the Gammaproteobacteia are reported for proteobacterial species found in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are also described in the species description section.

• Journal of Species Research
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• v.7 no.2
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• pp.161-180
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• 2018

Fourteen bacterial strains, low10-4-1, J11015, 17J27-22, 17G22-9, 17G9-4, 17Bio_15, 17gy_33, 17SD1_21, Strain8, 17Sr1_17, J21014T, H31021, 17J49-9, and 17J80-6 assigned to the phylum Actinobacteria, Bacteroidetes, Deinococcus-Thermus, and Firmicutes were isolated from soil samples. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strains low10-4-1, J11015, 17J27-22, 17G22-9, 17G9-4, 17Bio_15, 17gy_33, 17SD1_21, Strain8, 17Sr1_17, J21014T, H31021, 17J49-9, and 17J80-6 were most closely related to Marmoricola aurantiacus (98.9%), Calidifontibacter indicus (99.8%), Gordonia soli (98.8%), Rhodococcus globerulus (99.5%), Pseudarthrobacter siccitolerans (99.1%), Hymenobacter qilianensis (98.7%), Hymenobacter terrae (99.0%), Deinococcus yunweiensis (99.2%), Deinococcus proteolyticus (99.7%), Domibacillus indicus (99.2%), Exiguobacterium mexicanum (100.0%), Kurthia senegalensis (99.1%), Lysinibacillus composti (99.6%), and Bacillus loiseleuriae (99.3%). These fourteen species have never been reported in Korea, therefore we report them here for the first time.