• Journal of Soil and Groundwater Environment
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• v.14 no.5
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• pp.10-17
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• 2009

Bioremediation has been applied as a proven technology in remediation of TPH contaminated soil. However, the efficiency of biodegradation is dependent on temperature as microbial activity is depressed at lower temperature ranges ($30^{\circ}C{\sim}80^{\circ}C$). The objective of this study was to develop microbes with enhanced activities at the stated temperature conditions and to evaluate the remediation effectiveness of these microbes in TPH contaminated soil. Experiments were conducted to isolate hydrocarbon degradable microbial consortia cultured under different temperature conditions. It was found that there were 5 strains of mesophilic ($30^{\circ}C$) and 3 strains of psychrophilic ($80^{\circ}C$) microbes. The TPH concentration was reduced from 4,044 mg/kg to 1,084 mg/kg, (73.2%) in 10 days by using mesophilic microbial consortia and from 5,427 mg/kg to 1,756 (67.6%) in 50 days with psychrophilic microbial consortia in laboratory cultures under controlled conditions. This rate determination excluded physical degradation such as venting and dilution. A field study was then performed to examine the feasibility of applying these microbes in the land-farming process. In this case, 87.1% of the 2,560 mg/kg TPH contaminated soil was degraded in 56 days. The biodegradation rate coefficient (k) was $0.0374\;day^{-1}$. Findings of this study provide viable options for applying microbes for bioremediation of TPH in lower temperature conditions.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.3
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• pp.458-464
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• 2006

Bioremediation has been recognized as one of the best tools for hydrocarbon contaminated soil and nearby groundwater which had been heavily polluted in industrial areas. Degradation of PAHs in PAH-polluted loam soil were investigated under polyurethane foam environment with adsorbed bacteria Pseudomonas sp. (KCCM 40055) in order to acquire vital data for the environmentally-friendly process and material. macroporous commercial polyurethane foam that is widely used for microbial attachment in such as sewage treatment was selected for experiments. We also examined the microbial adherence upon the media. SR9-35C/G among the PU samples showed the highest degree of attachment and bioconversion. The conversion efficiency increased with moisture content of soil.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.5
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• pp.327-330
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• 2002

Two polyacrylamide degrading bacterial strains, No. 2 and No. 11, were isolated from soil, and identified as Bacillus sphaericus No.2 and Acinetobacter sp. No. 11, respectively. Both strains grew on medium containing polyacrylamide as sole carbon and nitrogen sources. B. sphaericus No. 2 and A. sp. No. 11 reduced by 16% and 19%of the initial polyacrylamide concentration, respectively. Optimal pH and temperature in growth of Acinetobacter sp. No. 11 were 8.0 and $37^{\circ}C$, respectively. After 14-day cultivation of A. sp. No. 11, the average molecular weight of polyacrylamide has been shifted from $2.3{\times}10^6\;to\;0.5{\time}106$.

• Journal of Applied Biological Chemistry
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• v.44 no.4
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• pp.194-198
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• 2001

Contaminated sites are often abandoned for an extended period of time, thus organopollutants becoming sequestered. The information on the initial concentrations of the contaminants would be helpful for the economic bioremediation of the chemicals. The present study estimated the initial concentrations of atrazine and phenanthrene through multiple regression analyses using soil properties and the amount of chemicals available in situ. Percentage mineralized or extracted was best correlated with organic C or logarithm of organic C, and the $R^2$ values were 0.548 and 0.894 for atrazine and phenanthrene, respectively. Estimation of the initial concentration of the chemicals was then calculated from both the percentage mineralized or extracted and the amount of chemicals extracted or mineralized. Results showed that the estimation of the initial concentration of the chemical at the time of contamination is feasible.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.04a
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• pp.260-265
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• 2004

Acid mine drainage(AMD) is one of the most serious environmental concerns associated with the mining industry around the world. The objective of this study is to assess the potential of sewage sludge as a carbon source for sulfate reducing bacteria and waste lime and steel slag as a neutralize agent for acid mine drainage bioremediation for use in permeable reactive materials. The study was performed using synthetic AMD in six column experiments. The effluent solution was systematically analysed throughout the experiments. The results of the study indicated that sewage sludge, waste lime and steel slag were the most effective for the AMD treatment as a permeable reactive materials.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2002.09a
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• pp.129-133
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• 2002

본 고에서는 지하 대수층 생태계 및 그에 대한 외국의 연구사례를 개괄하여 소개하고, 지속가능한 지하수 개발에 있어서 대수층 생물이 갖는 의미에 대하여 고찰하였다. 아울러 국내에서 발견된 대수층 생물을 소개하고 향후 연구방향을 설정하였다. 지하 대수층은 다양한 생물로 구성된 독특한 생태계를 가지고 있다. 생물학적 평형상태를 유지하는 경우 이 생태계는 대수층이 장기간 양호한 수질을 유지할 수 있는 기능을 가진다. Parabathynellidae, Parastenocaris 및 선충류의 발견을 통해서 고령군 다산면 대수층이 지하수 생태계 연구를 위한 적합한 대상임이 확인되었다. 향후 연구방향으로서 1) 지하수 동물상 조사, 2) 생태계 구성원에 대한 Autecology 및 Synecology적 접근, 3) Bioremediation 및 Bioindicator 관련 연구 등이 제시되었다.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.395-398
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• 2002

Bioremediation has been showing promise as an alternative to conventional environmental cleanup technologies. The objective of this study is to maximize the degradability of jet fuel in the soil system. The cells isolated from petroleum contaminated site was used for the degradation of jet fuel. When this strain was cultured in the MSM(minimal salt media) containing jet fuel for ten days, the degradability of jet fuel was almost 100%. The concentration of jet fuel did not affect the degradability much and the increased inoculution of strain and addition of nitrogen source decreased the time for complete degradation of jet fuel in the liquid culture. Inoculation of this strain increased the jet fuel degradability in the soil column by 15% and the aeration(50ml/min) and the addition of nutrients($NaNO_3$, $KH_2PO_4$) enhanced the jet fuel degradability(about 90%).

• Microbiology and Biotechnology Letters
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• v.41 no.1
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• pp.79-87
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• 2013

An unstable yet efficient phenanthrene-degrading bacterium strain Ph-3 was isolated from a petroleum-contaminated site at the Mathura Oil Refinery, India. The strain was identified as Pseudomonas sp. using a polyphasic approach. An analysis of the intermediates and assays of the degradative enzymes from a crude extract of phenanthrene-grown cells showed a novel and previously unreported pattern of 1, 2-dihydroxy naphthalene and salicylic acid production. While strain Ph-3 lost its phenanthrene- degrading potential during successive transfers on a rich medium, it maintained this trait in oligotrophic soil conditions under the stress of the pollutant and degraded phenanthrene efficiently in soil microcosms. Although the maintenance and in vitro study of unstable phenotypes are difficult and such strains are often missed during isolation, purification, and screening, these bacteria constitute a substantial fraction of the microbial community at contaminated sites and play an important role in pollutant degradation during biostimulation or monitored natural attenuation.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 1996.11a
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• pp.118-121
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• 1996

환경이 나이어린 학문 정도로 생각하는 것은 환경분야의 눈부신 발전과 연구를 모르는 데서 비롯된 오해이다. 산업화에 따른 산업재앙의 위기에. 처한 환경 선진국들은 이미 막대한 재정지원을 통해서 이미 오래전부터 최신 기법들을 이용한 실험들을 성공리에 마치고 있고 또 실제 생활에 활용하고 있다. 특히 환경오염에 대한 처리방법에 있어서 미국을 비롯한 선진국들은 유전공학을 이용한 최신기법들을 연구개발하고 있으며 이것은 매우 놀랍고 고무적인 일이다. 그런 의미에서, 이 글은 환경오염의 생물학적 처리복구기술에 있어서 DNA probe(DNA 탐침자)의 역할과 이 연구를 통한 미래환경발전의 전망에 대해 살펴보고자 한다.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2005.04a
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• pp.393-396
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• 2005

The reactive transport model on the biologically mediated sequential nitrate transformation and its subsequent transport was developed and tested. This model was coded as a reaction module within the RT3D framework (Clement, 1997). Transports of the reasonable six mobile solutes (dissolved organic carbon, $O_2,\;{NO_3}^-,\;{NO_2}^-,\;N_2O,\;N_2$) and two immobile microbes were simulated. The simulation results gave a reasonable match with supposed transport pattern. For the next step, the developed model will be validated against experimental data.