• The Korea Journal of Herbology
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• v.30 no.4
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• pp.71-79
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• 2015

Objectives : These present study was designed to investigate the relaxation effects of Alpiniae Oxyphyllae Fructus(AOF) on isolated corpus cavernosum smooth muscle.Methods : Rabbit corpus cavernous tissues were prepared in strip. Then relaxation responses of AOF at 0.01-3 ㎎/㎖ in contracted strips induced by phenylephrine(PE) were measured. To evaluate mechanisms, indomethacin(IM) tetraethylammonium chloride(TEA), Nω-nitro-L-arginine(_L-NNA), methylene blue(MB) were treated before AOF extract(0.1-3 ㎎/㎖) infused into precontracted strips induced by PE. And 1 mM Ca²⁺was infused into precontracted strips after pretreatment of AOF extract(3 ㎎/㎖) in Ca²⁺-free krebs-ringer solution. NO concentration was measured by Griess reagent system. Ratio of smooth muscles to collagen fibers and eNOS positive reaction were measured by histocheminal and immunohistochemical process.Results : The cavernous strips were significantly relaxed by AOF extract 0.1, 0.3, 1, 3 ㎎/㎖ and the pretreatment with IM 10 μM,_L-NNA 100 μM, MB 10 μM inhibited relaxation of AOF compared to non-pretreatment, but the pretreatment with TEA 100 μM didn't affect relaxation of AOF. In a Ca²⁺-free solution, pretreatment with AOF reduced increase on contraction of strips by Ca²⁺supply than non-pretreatment. On HUVEC, NO concentration was increased. On corpus cavernosum of penis in Spontaneous Hypertensive Rat, ratio of smooth muscles to collagen fibers and eNOS positive reaction in AOF group were increased compared to PE groupConclusions : Taken this results, we can suggest that AOF extract exerts a relaxation effects on rabbit corpus cavernosum smooth muscle in part by suppressing influx of extracellular Ca²⁺throughout prostacyclin, the NO-cGMP system.

• The Journal of Internal Korean Medicine
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• v.12 no.2
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• pp.74-88
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• 1991

In order to study the effects of Goosunsan known clinically for their effects of treatment for cough and asthmas the study was carried out to investigate the effect of Goosunsan extract on the contractile force of the isolated guinea pig's vaviovs kinds smooth muscles and elucidate its mechanism The result were obtained as follows: 1. The isolated trachea & ileum smooth muscles of guinea pig was suspended in the organ bath with oxygenated kreb's Henselsite bicarbonate buffer solution at $37^{\circ}C$, and the developed tension by the drug was recorded with Isometric transducer (nacro F-60). The resting tension was approximately 0.5g. 2. The isolated trachea & ileum smooth muscles of guinea pig was remakably relaxed by the administration of Goosunsan. 3. The contractile response of the trachea smooth muscle of the isolated guinea pig to histamine 10-4 M was remakably by Goosunsan extract. 4. The contractile response of the ileum smooth muscle of the isolated guinea pig to histamine 10-4 M was remakably by Goosunsan extract. 5. The contractile response of the trachea smooth muscle of the isolated guinea pig to acetylcholine 10-4 M was remakably by Goosunsan extract. 6. The contractile response of the ileum smooth muscle of the isolated guinea pig to acetylcholine 10-4 M was remakably by Goosunsan. 7. The contractile response of the trachea smooth muscle of the isolated guinea pig to 5-hydroxytryptamine 10-4 M was remakably by Goosunsan. 8. The contractile response of the ileum smooth muscle of the isolated guinea pig to 5-hydroxytryptamine 10-4 M was remakably by Goosunsan.

• The Journal of Internal Korean Medicine
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• v.14 no.1
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• pp.1-16
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• 1993

This study was carried out to investigate the effect of Oyootang and Shinchulsan extract on the contractile force of the isolated guinea pig trachea smooth muscle and elucidate its mechanism The result were obtained as follow: 1. The isolated trachea smooth muscle was suspended in the organ bath with oxygenated kerb's Henseleit bicarbonate buffer solution at $37^{\circ}C$, and the developed tension by the drug was recorded with isometric tranducer(Nacro F-60). The resting tension was approximately 0.5g 2. The contractile response of the trachea smooth muscle of the isolated guinea pig by histamine $10^{-4}M$ was significantly inhibited by Oyootang and Shinchulsan extract. 3. The contractile response of the trachea smooth muscle of the isolated guinea pig by acetylcholine $10^{-4}M$ was considerably inhibited by Oyootang and Shinchulsan extract. 4. The contractile response of the trachea smooth muscle of the isolated guinea pig by histamine $10^{-4}M$ was significantly inhibited by Oyootanghabshinchulsan extract. 5. The contractile response of the trachea smooth muscle of the isolated guinea pig by acetylcholine $10^{-4}M$ was significantly inhibited by Oyootanghabshinchulsan extract. 6. The contractile response of the trachea smooth muscle of the iso-lated guinea pig by histamine $10^{-4}M$ was significantly inhibited by Oyootang, Shinchulsan and its constituent herbs extract. 7. The contractile response of the trachea smooth muscle of the iso-lated guinea pig by acetylcholine $10^{-4}M$ was significantly inhibited by Oyootang, Shinchulsan and its constituent herbs extract.

• Journal of Physiology & Pathology in Korean Medicine
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• v.29 no.6
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• pp.485-491
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• 2015

This study was aimed to investigate the relaxation effects of Eucomiae Cortex (EC) extract in isolated rabbit corpus cavernosum smooth muscle and its mechanism. To evaluate the relaxation of EC extract in rabbit corpus cavernosum, EC extract was treated in corporal strips which were precontracted with phenylephrine(PE). To study its mechanism, Nω-nitro-L-arginine (L-NNA) was pretreated after infuse of EC extract and compared with non-treated. In calcium chloride (Ca²⁺) -free krebs solution, EC extract and Ca²⁺ 1 mM were infused by turns after Ca²⁺ 1 mM was treated into corporal strips contracted by PE. Cell ability, nitric oxide (NO) and epithelial nitric oxide synthase (eNOS) on human umbilical vein endothelial cell (HUVEC) were measured by MTT assay, Griess reagent system and histochemical, immunohistochemical methods. EC extract showed a significant relaxation effects on the corporal strips, this effects were inhibited by pretreatment of L-NNA. EC extract inhibited the increase of contraction by Ca²⁺ influx in Ca²⁺-free krebs solution, and eNOS positive reaction in corpus cavernosum, NO production in HUVEC increased by treatment of EC extract. These result suggest that the relaxation effects of EC extract in isolated corpus cavernosum smooth muscle are involved in increase of eNOS and NO production, blocking of extracellular Ca²⁺ influx.

• Journal of applied mathematics & informatics
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• v.4 no.2
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• pp.477-485
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• 1997

Let E be a smooth Banach space. Suppose T:$E \rightarrow E$ is a strongly accretive map. It is proved that each of the two well known fixed point iteration methods (the Mann and ishikawa iteration methods), under suitable conditions converges strongly to a solution of the equation $T_x=f$.

• Communications of the Korean Mathematical Society
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• v.13 no.4
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• pp.765-773
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• 1998

Let E be a smooth Banach space. Suppose T : E longrightarrow E is a strongly accretive map. It is proved that each of the two well known fixed point iteration methods (the Mann and Ishikawa iteration methods), under suitable conditions, converges strongly to a solution of the equation Tx = f.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.16 no.2
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• pp.21-27
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• 2008

An inverse method is introduced to construct the problem for the error analysis of the numerical solution of initial value problem. These problems constructed through this method have a known exact solution, even though analytical solutions are generally not obtainable. The process leading to the exact solution makes use of an initially available approximate numerical solution. A smooth interpolation of the approximate solution is forced to exactly satisfy the differential equation by analytically deriving a small forcing function to absorb all of the errors in the interpolated approximate solution. Using this special case exact solution, it is possible to investigate the relationship between global errors of a candidate numerical solution process and the associated tuning parameters for a given problem. Under the assumption that the original differential equation is well-posed with respect to the small perturbations, we thereby obtain valuable information about the optimal choice of the tuning parameters and the achievable accuracy of the numerical solution.

• Journal of applied mathematics & informatics
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• v.29 no.3_4
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• pp.969-980
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• 2011

In this paper, our main purpose is to consider the quasilinear elliptic equation $$div(|{\nabla}u|^{p-2}{\nabla}u)=(p-1)f(u)$$ on a bounded smooth domain ${\Omega}\;{\subset}\;R^N$, where p > 1, N > 1 and f is a smooth, increasing function in [0, ${\infty}$). We get some estimates of a solution u satisfying $u(x){\rightarrow}{\infty}$ as $d(x,\;{\partial}{\Omega}){\rightarrow}0$ under different conditions on f.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2002.10a
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• pp.58-63
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• 2002

This study has performed rotary bending fatigue testing that smooth specimen using SM45C materials and notched specimen whose radii were R6, R4 and R2, were processed in 3% NaCl aqueous solutions or in the air. The results are as followed; 1. In the air fatigue limit at 10$^{7}$ cycles remarkably reduced as notch radius goes small. 2. In 3% NaCl aqueous solution fatigue strength at 10$^{6}$ cycles also had large range of reduction as notch radius goes small. 3. Comparing fatigue strength in the air, fatigue strength at 10$^{6}$ cycles in 3% NaCl aqueous solution reduced by 46.2% at smooth specimen, 55.3% at R6 notch radius, 45.8% at R4 and 39.7% at R2 respectively. 4. The reason that fatigue strength reduced in the reduction of notch radius sire was because the surface of notch exposed in corrosion was small and consequently it was less corroded.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.1
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• pp.147-150
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• 2007

Electro-deposition of carbon film on silicon substrate in methanol solution was carried out with various current density, solution temperature and electrode spacing between anode and cathode. The carbon films with smooth surface morphology and high electrical resistance were formed when the distance between electrode was relatively wider. The electrical resistance of the carbon films were independent of both current density and solution temperature.